The activity of the lithium—sodium countertransport system in erythrocytes in depression and mania

The activity of the lithium-sodium countertransport system in erythrocytes was estimated in 16 control subjects and 28 patients suffering from affective disorders (11 bipolar, 17 unipolar) during depression, mania and remission. In all the patients studied, mean estimates of countertransport were significantly lower during an episode than after remission, with low values occuring more frequently in mania than in depression in bipolar patients. The mean values in patients with affective disorders but who were in remission remained lower than those of control subjects. Lowest values occured more frequently in patients with bipolar than in those with unipolar illness. The possible physiological significance of reductions in the activity of the lithium-sodium countertransport mechanism in red cells was discussed.     

Simultaneous occurrence of 5q− and 21q− in refractory anemia with thrombocytosis

A 40-yr-old female with refractory anemia and thrombocytosis was shown to possess 5q? and 21q? chromosomal anomalies in the hematopoietic cells. The former anomaly was demonstrated to be a del(5)(q14q32) and the latter to be due to a t(11;21)(q25;q21). A similar translocation was shown to exist in the cells of another patient with essential thrombocytosis. Thus, we tentatively identify the 21q? as being due to a t(11;21).     

Characterization of the 33-kilodalton major allergen of Penicillium citrinum by using MoAbs and N-terminal amino acid sequencing

Background The 33 kD component has been identified as a major allergen ofPenicillium citrinum, the most prevalent Penicillium species in the Taipei area of Taiwan. Objective This study analyses the isoforms, antigenic cross-reactivity and the N-terminal amino acid sequence of the 33 kD allergen of P. citrinum. Methods The composition of isoforms and antigenic cross-reactivity was analysed by SDS-PAGE and 2D-immunobIotting using MoAbs generated. The N-terminal sequence was analysed by using an automatic gas/liquid phase sequencer. Results Two MoAbs (55A and 34H) against the 33 kD allergen were generated in the present study. In addition to the 33 kD component. MoAb 34H also showed immunoblot reactivity to other components in the crude extract of P. citrinum. Analysed by 2D-immunoblotting. at least six different isoforms of the 33 kD component with pl values ranging from 6.75 to greater than 7.0 were shown to be reactive to both MoAbs and IgE antibodies in serum of an asthmatic patient. Different immunoblot patterns were observed when both MoAbs were reacted with four different strains of P. citrinum used in the present study. Among another six different Penicillium and four different Aspergillus species tested, only an immunoblot reactivity of MoAb 55 A to the 33 kD component of P. brevicompactum was observed. In 2D-immunoblotting. components of P. brevicompactum with an MW of about 33 kD and pi values similar to those of the 33 kD component of P. citrinum reacted with MoAb 55A and IgE antibodies in serum of the asthmatic patient. The N-terminal amino acid sequence of the 33 kD component of P. citrinum was determined to be ANVVQSNVP which was identical to the first 9 N-terminal amino acids of a heat-labile alkaline serine proteinase from P. citrinum. Conclusion Results obtained in the present study suggest that the 33 kD major allergen of P. citrinum may be an alkaline serine proteinase.     

Automated polymerase chain reaction for papillomavirus screening of cervicovaginal lavages: comparison with dot-blot hybridization in a sexually transmitted diseases clinic population

The aim of the present study was to compare the recently developed polymerase chain reaction (PCR) technique with conventional dot-blot DNA hybridization for human papillomavirus (HPV) detection. Cells were collected by cervicovaginal lavage from a study group of 109 women attending a sexually transmitted diseases clinic. Using a machine that we developed for alternation of temperature cycles, HPV was detected in 51% of patients by PCR. By dot-blot hybridization, 44% of the patients were positive. Concordance of combined positive and negative results between PCR and dot blot was 69%. The greater sensitivity of PCR may have accounted for 19% of specimens that were PCR positive but dot-blot negative. Unexpectedly, however, 12% of specimens were dot-blot positive but negative by PCR, and several specimens were discordant for type of HPV. Both HPV DNA tests agreed with cytology in 41% of women, and in 33% cytology was negative in the face of positive PCR and dot blot. Concordance of cytology with just PCR was 59%, and only with dot blot was 56%. Cervicography agreed with both HPV DNA tests in 41% of patients, with PCR alone in 55%, and with dot blot alone in 58%. Biopsy results did not reveal a strong correlation between histopathological criteria of HPV infection and detection of HPV DNA by either PCR or dot-blot hybridization. Thus the present study has shown that PCR is a slightly more sensitive indicator of HPV infection than dot-blot hybridization. Agreement of HPV DNA results with conventional screening tests was not strong, an observation consistent with many comparative studies by others. In conclusion, PCR is slightly more sensitive than DNA hybridization for detection of HPV, it can be used in conjunction with specimen collection by gentle lavage of the cervicovaginal epithelium, and the possibility remains that it may prove suitable as a screening test.     

非酒精性脂肪肝患者血清miR-122的表达

目的探讨非酒精性脂肪肝(NAFLD)患者血清miR-122的表达水平,探索新的诊断NAFLD的生物学标记。方法选择临床确诊的NAFLD患者48例,健康对照组48例。留取血清,用实时荧光定量PCR方法检测血清miR-122的表达。统计学分析miR-122在两组间表达水平的差异。结果NAFLD患者血清miR-122的表达水平较对照组明显升高(P0.01)。结论miR-122可能参与了NAFLD的发病机制,有可能成为NAFLD新的诊断标记物。     

白细胞介素-33和血管内皮生长因子C在胃癌中的表达及临床意义

目的 探讨白细胞介素-33(IL-33)和血管内皮生长因子C(VEGF-C)在胃癌患者组织标本及血清中表达,以及二者与胃癌淋巴结转移的关系.方法 分别应用免疫组织化学SP法和酶联免疫双抗夹心法(ELISA)检测98例胃癌患者和36名健康体检者胃黏膜组织标本及血清中IL-33和VEGF-C水平.结果 胃癌组织中IL-33和VEGF-C的阳性表达率分别为67.35％和74.49％,显著高于正常胃组织(47.22％和61.11％),比较差异有统计学意义(P<0.01).IL-33和VEGF-C表达在不同的肿瘤分化程度、组织浸润、淋巴结转移、远处转移及临床分期间比较差异有统计学意义(P<0.05).胃癌淋巴结转移患者的IL-33和VEGF-C的表达阳性率高于未发生淋巴结转移患者(P<0.05).胃癌患者血清IL-33和VEGF-C水平为(50.24±13.08)pg/mL和(210.73±58.35)pg/mL,高于健康体检者(P<0.05);淋巴结转移患者血清IL-33和VEGF-C水平高于未发生淋巴结转移患者,比较差异有统计学意义(P<0.05).结论 胃癌患者血清中高水平IL-33可能诱导VEGF-C分泌,促进胃癌淋巴结转移,可作为评估胃癌预后的重要指标.     

miR-133b调控GSTP1表达降低结肠肿瘤细胞在低营养环境下的生存能力

目的 探究GSTP1在不同阶段结肠癌组织中的表达水平,并查找可能对GSTP1表达产生调控作用的微小分子RNA（miR）及其对结肠肿瘤细胞的影响。方法 利用实时定量PCR和蛋白印迹分析检测GSTP1在结肠癌组织中的表达水平;构建载体pcDNA3.1GSTP1并转染到培养的人类结肠癌细胞系HCT116中,检测细胞的凋亡率与活性水平;利用TargetScan 和MicroCosm Targets程序分析GSTP1的3′UTR序列并查找能够结合GSTP1的miR,构建载体pGL3GSTP13′UTR,共转染该miR与pGL3GSTP13′UTR到人类肾脏上皮细胞系HEK293中,荧光素酶报告分析验证该miR对GSTP1表达的靶向调控作用;转染该miR到HCT116中,检测GSTP1表达水平与细胞的活性氧（ROS）水平;共转染该miR与pcDNA3.1GSTP1到HCT116中,检测细胞的ROS水平与活性水平。结果 GSTP1在不同阶段结肠癌样本中的表达水平均上升,在低营养环境下,高表达GSTP1的HCT116细胞凋亡率下降,细胞活性上升。而miR133b在GSTP1的3′UTR序列区域有特异性结合位点,在转录后水平下调GSTP1的表达。在低营养环境下,过表达miR133b的HCT116细胞活性水平显著下降,ROS水平显著上升;在正常营养环境下,高表达miR133b无显著影响。此外,在低营养环境下,过表达miR133b的HCT116细胞同时过表达GSTP1,则细胞活性水平下降程度和ROS水平上升程度均减小。结论 低营养环境下miR133b能够通过对GSTP1的抑制作用削弱结肠肿瘤细胞的生存能力。     

岐黄针疗法治疗膝骨性关节炎的临床疗效观察

【目的】观察岐黄针疗法治疗膝骨性关节炎的临床疗效。【方法】将60例膝骨性关节炎患者随机分为治疗组和对照组各30例。治疗组采用岐黄针疗法治疗(主要选取膝阳关、曲泉、委中等穴位),对照组采用常规针刺疗法治疗。经过10 d治疗后,以简化麦吉尔疼痛问卷(MPQ)和Lysholm膝关节评分标准为观察指标,观察2组临床疗效。【结果】(1)治疗10 d后,2组患者膝关节疼痛、肿胀、僵硬、屈伸不利等症状均有好转,MPQ量表中的疼痛分级指数(PRI)、视觉模拟评分(VAS)和现时疼痛强度评分(PPI)均有显著性降低,而Lysholm膝关节评分则均有显著性提高(P0.01),且治疗组在降低MPQ各项评分及提高Lysholm膝关节评分方面均优于对照组(P0.05)。(2)治疗组总有效率为86.67%,对照组为66.67%,治疗组疗效优于对照组,差异有统计学意义(P0.05)。【结论】岐黄针疗法治疗膝骨性关节炎疗效确切,具有疗程短、疗效佳、操作简便等优点。     

哮喘急性发作患者血清中IL-18、IL-33表达意义及其与肺功能的关系

目的 探讨IL-33、IL-18表达水平与支气管哮喘急性发作之间的相关性。方法 选取上海普陀中心医院2014年5月至2014年11月收治的80例哮喘急性发作患者为哮喘观察组研究对象,同时选取同期来我院常规健康体检的80例健康体检者对照组研究对象。采用酶联免疫吸附法(ELISA)测定所有研究对象血清中IL-33和IL-18水平并做比较。78例哮喘患者康复出院后2周复查肺功能测定,比较患者住院治疗时肺功能并分析其与IL-18、IL-33相关性。结果哮喘组患者治疗前IL-18水平为(310.2±152.6)pg/ml,IL-33水平为(215.4±87.5)pg/ml,较健康组(204.2±95.4)pg/ml、(138.7±48.3)pg/ml偏高,差异具有统计学意义(P<0.05);哮喘组患者治疗后血清中IL-18水平为(215.5±98.2)pg/ml,IL-33水平为(146.6±54.9)pg/ml,较治疗前降低,差异具有统计学意义(P<0.05);健康组肺功能检查: FEV₁、FEV₁% pred和FEF50%值优于哮喘组,治疗后哮喘组FEV₁、FEV₁% pred和FEF50%等值优于治疗前,且经线性回归分析,IL-18和IL-33与FEV₁% pred等肺功能指标呈负性相关关系。结论哮喘急性发作患者血清中IL-18与IL-33表达水平较正常人高,治疗后两者水平均明显下降。两者与肺功能呈负相关。