IL-6、IL-12、IL-33在丙型肝炎患者中的表达及其临床意义

目的探讨丙型肝炎患者血清中IL-6、IL-12及IL-33含量的变化及其临床意义。方法ELISA法检测30例丙型肝炎患者、30例丙型肝炎病毒携带者及30例健康对照者血清中IL-6、IL-12及IL-33的表达水平。结果相比丙型肝炎病毒携带者及健康对照者,丙型肝炎患者血清中IL-6、IL-12及IL-33的表达水平显著升高（P〈0．01）,且丙型肝炎患者血清中IL-6、IL-12及IL-33的含量显著高于丙型肝炎病毒携带者（P〈0．01）;经治疗后丙型肝炎患者血清中IL-6、IL-12及IL-33的含量显著降低（P〈0．01）。结论IL-6、IL-12及IL-33介导的免疫应答参与了丙型肝炎的发病,在丙型肝炎病毒感染的致病机制中有-定的临床价值。     

Hybrid Melody Valve Implantation in Mitral Position in a Child: Usefulness of a 3-Dimensional Printed Model for Preprocedural Planning

We present the case of a 4-month-old child with atrioventricular canal associated with severe left atrioventricular valve dysfunction who previously underwent 3 surgical valve reconstructions without significant improvement. A Hybrid Melody valve (Medtronic, Minneapolis, MN) insertion was planned. Because of the low weight, the risk of left ventricular outflow tract obstruction was significant and therefore evaluated preprocedurally using a cardiac computed tomography-derived 3-dimensional printed model. In vitro tests showed good anchorage of the valve without subaortic obstruction and the procedure was then achieved with an excellent clinical result.     

3D 打印导板辅助空心螺钉内固定治疗不稳定性骨盆骨折

目的评估 3D 打印导板辅助空心螺钉内固定术治疗不稳定性骨盆骨折的疗效。方法回顾分析 2015 年 5 月—2016 年 6 月采用 3D 打印导板辅助空心螺钉内固定术治疗的 10 例不稳定性骨盆骨折患者临床资料。男 7 例，女 3 例；年龄 20～58 岁，平均 37.5 岁。致伤原因：高处坠落伤 5 例，重物压伤 1 例，交通事故伤 4 例。伤后至入院时间 1～5 h，平均 3.1 h。骶骨骨折 6 例，右侧骶髂关节脱位 1 例，髂骨骨折 3 例；10 例均有耻骨上下支骨折，左侧 3 例（其中 2 例耻骨上支骨折邻近耻骨联合），右侧 2 例，双侧 5 例。骨折 Tile 分型：B2 型 4 例，B3 型 1 例，C1 型 4 例，C2 型 1 例。术后根据 Matta 评分标准进行影像学评估，根据 CT 三维重建评估骶髂螺钉和耻骨上支螺钉位置，按照 Majeed 功能评分进行功能评估。结果患者平均每枚螺钉植入时间为 30 min，平均每枚螺钉切口失血量为 50 mL。每枚骶髂螺钉植入透视时间为 24～96 s，平均 62 s；每枚耻骨上支螺钉植入透视时间为 42～80 s，平均 63.2 s。住院时间 17～90 d，平均 43.7 d。术后患者切口均Ⅰ期愈合。患者均获随访，随访时间 12～22 个月，平均 15.6 个月。术后 1 d 根据 Matta 评分标准评定获优 8 例，良 2 例；CT 三维重建示 9 枚骶髂螺钉位置均为Ⅰ类，13 枚耻骨上支螺钉位置均为 0 级。随访期间均未出现血管神经损伤，螺钉退出、断裂，继发性骨折移位等并发症。术后 6 个月 X 线片示骨折均已愈合。术后 1 年根据 Majeed 功能评分评价功能，获优 9 例，良 1 例。末次随访时，合并左侧 L₅ 神经根损伤的 Tile C2 型患者 L₅ 神经根功能恢复正常。 结论3D 打印导板辅助空心螺钉内固定术治疗不稳定性骨盆骨折有助于精确植入螺钉、降低血管神经损伤风险，是治疗不稳定性骨盆骨折的一种较好选择。     

Isolation and characterization of Entamoeba histolytica mutants resistant to emetine

Isolation of emetine-resistant mutants of Entamoeba histolytica is described. Spontaneous rate of mutation, obtained from a Luria-Delbrück fluctuation test was 2.5 X 10(-7). The mutagenesis frequency obtained from the number of colonies grown in the presence of emetine, divided by the number of viable trophozoites inoculated in semisolid agar was less than or equal to 10(-7) and it was increased from 10 to 100 fold with ethyl methanesulfonate. Two types of clones were isolated: one was cross-resistant to colchicine and the other was not, indicating that mutation took place at different loci. Protein synthesis in the presence of emetine remained unaltered for colchicine-sensitive mutants, suggesting that the molecular lesion lay in the protein synthesis machinery. Colchicine-resistant mutants showed a lower level of [35S]methionine incorporation, probably due to alterations in the molecular transport of some substances across the membrane. This mutant clone was also deficient in red blood cell adhesion, suggesting membrane alterations.     

表面活性剂对GD_3合成酶活性的影响及增溶作用

应用不同类型的表面活性剂系统研究其对大鼠肝癌GD_3合成酶(CMP-唾液酸-GM_3唾液酸转移酶,ST2)活性的影响及增溶作用。研究结果表明Triton系列的表面活性剂增溶作用较好,在0.1mol/L KCl存在下Tritoa CF-54和Triton X-100(1:1V/V)两者混溶时,其增溶效果比其单独使用更佳,非离子型表面活性剂NP-40和Triton CF-54具有最大的激活作用。当离子型表面活性剂SDS的胆酸钠浓度在0.1％～0.2％时对ST2略有激活作用,面大于0.4％时有抑制作用。不同的表面活性剂对SF2活性产生不同的影响,提示SF2与脂结合的结构区域,与表面活性剂或脂结合时对ST2活性具有调节作用。