佛甲草对小鼠实验性肝损伤的保护作用

目的：观察佛甲草对小鼠实验性肝损伤的保护作用。方法：分别采用四氯化碳（CCl4）、α-萘异硫氰酸酯（ANIT）一次性灌胃中毒致小鼠急性肝损伤及黄疸模型，测定血清谷丙转氨酶（ALT）、总胆红素（TBiL）含量及血清、肝组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、一氧化氮（NO）水平和观察肝组织病理变化，分析佛甲草对上述指标的影响。结果：佛甲草能明显降低CCl4中毒性小鼠血清ALT、血清及肝组织中MDA含量（P〈0.01），使SOD，NO水平显著增高（P〈0．01，P〈0．001）；能明显降低ANIT中毒性小鼠血清TBil含量（P〈0．001）；肝脏病理组织学检查表明佛甲草能明显减轻肝细胞的变性和坏死。结论：佛甲草对CCl4和ANIT所致的急性肝损伤有保护作用，其机理可能与抗脂质过氧化及改善肝脏微循环，恢复肝脏功能的作用有关。     

Effect of chronic alcohol consumption on plasma lipid, vitamins A, and E in Korean alcoholics

This human study was conducted to evaluate the effect of chronic alcohol consumption on plasma concentrations of lipid and the antioxidative system in 44 Korean alcoholics and 45 age-, sex-, and nationality-matched nonalcoholic subjects. Plasma triacylglycerols and atherogenic index were higher in alcoholics than in control subjects. Plasma total cholesterol was not different among groups, but plasma high-density lipoprotein cholesterol was lower in alcoholics. There were positive correlations between ethanol consumption and plasma lipid peroxide and atherogenic index in all subjects; there were negative correlations between ethanol consumption and plasma high-density lipoprotein cholesterol in all subjects. There were no significant differences between alcoholics and control subjects in plasma concentrations of α-tocopherol, although plasma α-tocopherol/lipid tended to be lower in alcoholics. Plasma retinol was lower in alcoholics. These results suggest that chronic ethanol consumption can contribute to increased risk for vascular diseases in Korean alcoholics.     

人正常宫颈鳞状上皮基因表达模式的研究

目的　研究人正常宫颈鳞状上皮基因表达模式。　方法　应用基因表达系统性分析方法 (serial analysis of gene expression,SAGE)建立正常宫颈鳞状上皮基因表达的 SAGE文库。　结果　经 SAGE分析获得 2 0 880个特异标签 ,从中得到 2 935个特异基因信息 ,93.6 %基因呈低表达 ,其余 6 .4 %基为呈中、高表达。　结论　宫颈的鳞状上皮细胞中有众多的基因表达 ,其中绝大多数基因呈低表达 ,只有为数很少的基因呈高表达 ,对今后宫颈癌发病机制的研究具有重要意义。     

Expression Profile of Metastasis-associated Genes in Esophageal Squamous Cell Carcinoma

Esophageal cancer (EC) is one of the mostcommon fatal cancers in the worldwide , and ischaracterized by its high metastasis ,local lymphnode and blood metastasis at early stage , whichmay be the key factors of its recurrence and poorprognosis[1]. Thus ,better elucidation of molecularmechanism during metastasis is expected to i m-prove prevention and treat ment of EC. Many stud-ies have contributed to advance inits molecular as-pects . This li mited information based on singlegene ,however ,i…     

Difference of Gene Expression Profiles between Barrett’s Esophagus and Cardia Intestinal Metaplasia by Gene Chip

Theincidenceofadenocarcinomaintheesoph agusandgastroesophagealjunction(GEJ)isin creasingforthetwodecadesinNorthAmericaandEurope[1].Barrett'sesophagus(BE)isthoughttobeapremalignantconditionforesophagealadeno carcinomaandmostofadenocarcinomasatGEJ[2].Recentlythepresenceofcardiaintestinalmetapla sia(CIM)insomenormalappearingGEJhasbeendescribed[3].TherelationofthisconditiontoBEhasnotyetbeeninvestigated.Inthisexperiment,wehadperformedoura nalysisonthree4096chipsinordertoacquirethedifference…     

脂宁胶囊对高脂大鼠血脂水平的影响

目的探讨脂宁胶囊对高脂饲料饲养大鼠血脂水平的影响。方法将大鼠按基础血清总胆固醇水平将动物随机分为4组:高脂对照组、低、中、高剂量实验组。实验期间,所有大鼠均饲以高脂饲料,低、中、高剂量组分别灌胃给予0.0835、0.1670、0.5010 g/kg.bw剂量的脂宁胶囊,对照组以蒸馏水灌胃,灌胃量1 ml/100 g.bw,连续30 d后,测定血清总胆固醇(TC)、甘油三酯(TG)和高密度脂蛋白胆固醇(HDL-C)。结果0.16700、.5010 g/kg.bw剂量可显著降低大鼠血清TC水平(P<0.05),0.0835、0.1670 g/kg.bw剂量可显著降低大鼠血清TG水平(P<0.05),0.1670、0.5010g/kg.bw剂量升高HDL-C水平达4 mg/dl以上。结论脂宁胶囊具有辅助调节血脂作用。     

志贺菌质粒图谱及耐药谱分析

目的了解深圳市龙岗区志贺菌耐药及质粒谱分布情况。方法对分离来自全区各镇医院的70株志贺菌流行株应用纸片扩散法测定细菌对14种抗生素的敏感性,并采用Kado法进行质粒抽提。结果龙岗区志贺菌耐药情况严重,多重耐药普遍(57.1%),耐药谱型分散,计有36种耐药谱型;共检出8种不同相对分子质量的质粒,120和140 Md单质粒检出率较高,分别为15.7%和54.28%;质粒谱型有12种,但以120和140 Md单质粒谱型为主,合计占69.98%。结论将耐药谱型与质粒谱型进行比较,具有相同质粒谱型的菌株,其耐药谱型可相同,亦可不同,两者关系有待进一步探讨。结合文献报道,估计120 Md是志贺菌毒力质粒,并推测140 Md是否为该地区的R质粒。     

药物载体脂肪乳的研究与应用

综述作为药物载体的脂肪乳的分类、制备方法和工艺、存在的问题和解决方法、应用及发展前景。以甘油三酯和磷脂为主要成分的脂肪乳除可用作肠外营养外,近年来其用作药物载体的研究备受关注。     

针刺对烧伤创面组织LPO、SOD的影响

目的:探讨针刺促进烧伤创面愈合的机理.方法:建立兔深Ⅱ度烧伤动物模型,电针双侧"足三里"穴,同时取正常组织对照.动态观察烧伤后早期创面组织中脂质过氧化物(LPO)和超氧化物歧化酶(SOD)的变化以及针刺"足三里"穴后对创面组织中LPO、SOD的影响.结果:烧伤创面组织早期SOD的活性下降,LPO升高.针刺后可提高烧伤后早期组织抗过氧化能力.结论:针刺可促进烧伤创面愈合,其机理可能是针刺可提高烧伤组织抗过氧化能力所致.     

黄芩苷对局灶性脑缺血大鼠脑组织基因表达谱的影响

目的 :研究黄芩苷对局灶性脑缺血大鼠脑组织基因表达谱的影响 ,探索黄芩苷治疗脑缺血的药理作用机制。方法 :分别从假手术组、局灶性脑缺血组、黄芩苷治疗组SD大鼠的脑组织中抽提总RNA ,Cy3 ,Cy5荧光标记 ,反转录分别合成cDNA探针后 ,与含有 4096条大鼠基因的BioStar基因表达谱芯片杂交 ,AxonGenepix 40 0 0B扫描仪扫描 ,GenePixPro 3 .0软件分析表达信号。结果 :大鼠局灶性脑缺血组差异表达的基因有 211条 ,其中 12条基因上调 ,199条基因下调。黄芩苷治疗后差异表达的基因有 177条 ,其中有 89条基因上调 ,而 88条基因下调。进一步分析发现 ,1个在模型组下调的基因经黄芩苷治疗后上调 ,3个在模型组上调的基因经黄芩苷治疗后下调 ,3个在模型组上调的基因经黄芩苷治疗后表达进一步增强。结论 :在基因组水平上黄芩苷可能通过多基因 ,多途径调节大鼠脑缺血基因表达谱而发挥药理作用。