视网膜母细胞瘤Rb50细胞株VEGFR-3表达的研究

目的:研究视网膜母细胞瘤细胞株VEGFR-3的表达,探讨视网膜母细胞瘤自身增殖的机制.方法:选择Rb50细胞株,通过S-P免疫组化法、RT-PCR法及PCR产物琼脂糖凝胶电泳等方法,研究Rb50细胞株VEGFR-3的表达情况.结果:S-P免疫组化法呈阳性结果,表明Rb50细胞株有VEGFR-3的表达,RT-PCR产物琼脂糖凝胶电泳表明Rb50细胞株有较高水平VEGFR-3 mRNA的存在.结论:视网膜母细胞瘤细胞株有VEGFR-3的表达,表明VEGFR信息通路对视网膜母细胞瘤生长、浸润的影响,除了与促进肿瘤血管及淋巴管新生,改善肿瘤组织微环境相关外,还与促进肿瘤细胞自身增殖有关.     

多媒体教学对大学生视、听功能影响的调查分析

目的从医学生理角度探讨多媒体教学对大学生视、听功能的影响。方法依据眼疲劳及耳疲劳的主要症状自行设计调查表,对2004级、2005级临床医学专业应用多媒体教学的班级进行调查分析。结果2004级、2005级均有部分学生报告有眼疲劳及耳疲劳症状。2004级2个班眼疲劳及耳疲劳发生情况对比各项指标差异无显著性;2005级2个班眼疲劳及耳疲劳发生情况对比各项指标差异也无显著性;2004级与2005级眼疲劳及耳疲劳发生情况对比,5项指标差异具有显著性,5项指标差异不具有显著性。结论多媒体教学对大学生视、听功能的影响不容忽视,要采取积极的预防措施。     

稳定敲低MYH10基因细胞株的建立

目的 构建稳定敲低MYH10基因的COS-7细胞株。方法 在293TX细胞中进行病毒的包装,用获得的高效价慢病毒感染COS-7细胞,通过免疫印迹和荧光显微镜,检测病毒感染后COS-7细胞MYH10蛋白表达的变化。结果 在构建的两种重组质粒中,MYH10-lentiviral shRNA-2在转染后表现出明显的干扰作用,感染后能明显下调COS-7细胞的MYH10蛋白表达。结论 病毒感染的COS-7细胞可明显抑制COS-7细胞内源MYH10的表达,说明了稳定敲低MYH10基因的COS-7细胞株的建立,为进一步研究MYH10在相关疾病中的功能奠定了基础。     

浅谈抗菌药物管理信息系统建设

以卫生部颁布规范为基础进行我院抗菌药物管理信息系统设计和实施,并在此基础上结合医院信息系统和应用环境,实现了相应的信息系统功能及配套具备消息机制平台和抗菌药物控制细化扩展功能。经过近1年使用,建立了整合医院信息资源的平台,并利用其中的扩展功能为抗菌药物进一步深入管控做出了有益的探索。     

Research on Dynamic Strength and Inertia Effect of Concrete Materials Based on Large-Diameter Split Hopkinson Pressure Bar Test

The Split Hopkinson Pressure Bar (SHPB) test device is an important tool to study the dynamic characteristics of concrete materials. Inertial effect is one of the main factors that cause inaccurate results in SHPB tests of concrete materials. To solve this problem, Large-diameter SHPB tests on concrete and mortar were performed. A dynamic increase factor (DIF) model considering strain rate effect and inertia effect was established. This model provides a scientific reference for studying the dynamic mechanical properties of concrete materials. The experimental results indicate that the strain rate effect of concrete is more sensitive than that of mortar, but the inertia effect of mortar is more sensitive than that of concrete. Under the same strain rate, the energy utilization rate, average fragment size, and impact potentiality of mortar are higher than concrete.     

NOTES ON THE SUPPRESSON OF BUSHFIRES FROM AIRCRAFT

The paper presents a review of pole utilization in Australia. Commercial pressure treatment of poles commenced in 1987.

The service life of poles based on their natural durability is discussed, together with a brief consideration of the advantages of wooden poles compared with those of other materials. Attention is drawn to the change in specifications resulting from the rising demand for pole timber suitable for preservation, and to the importance of knots in Pinus species, in relation to impregnation and strength.

An outline is given of present pole preservation treatment in Australia and current problems are briefly discussed—splitting of certain species, slow drying and the possibility of developing artificial seasoning, low retention of preservatives and variations in absorption between poles in a charge in hardwoods and fungal attack, strength, and knots in softwoods.

The paper is designed to provide Australian foresters with an outline cf aspects of pole utilization which require to be taken into consideration for forest management.     

NEW SOIL CONSERVATION ACT IN VICTORIA

The breeding programme at Traralgon aims to improve Eucalyptus regnans F. v. M. as a forest tree by increasing the rate of growth, improving wood quality, and increasing resistance to damage by fire. Improvement may be accomplished in three ways:—(1) provenance trials (to determine the best place to collect seed for afforestation in a given location), (2) selection of individual trees within the chosen provenance, and (3) hybrids with species with greater resistance to fire and the ability to grow on poor sites. The major part of the work has been the provenance trials which show continuous genetic variation with altitude on the south eastern side of Mount Erica, Victoria.

Seed for the trials was collected in October 1959 from 68 trees on Mount Erica. with an average of 10 trees at each of seven altitudes from 1200 feet to 3250 feet. Studies were made of capsule size, germination, cotyledon area, and growth of seedlings in the nursery. Sixty-four open pollinated progenies were planted in a cubic lattice design in the Strzelecki Ranges at 1650 feet, and some smaller trials were planted at other places. Field measurements at two years of age and again at three years confirmed the trend shown in the nursery that seedlings from higher altitudes grew more slowly. Leaf shape also varied with altitude but survival of seedlings did not. A study of the relation of nursery measurements to field measurements showed promise of a technique for culling the slowest growing progenies in the nursery without the need for field planting all the progenies.

An examination of growth rates of progenies from a given altitude showed that large dominant trees rarely produced the fastest growing seedlings, consequently trees selected for breeding should not be simply the largest individuals but rather the parents which give the best progeny in nursery and field trials.

Experiments were commenced on the value for forestry of hybrids of E. regnans and some observations were made on seed production.     

Mumps Hoshino and Torii vaccine strains were distinguished from circulating wild strains

Aseptic meningitis and acute parotitis have been observed after mumps vaccination. Mumps outbreaks have been reported in Japan because of low vaccine coverage, and molecular differentiation is required to determine whether these cases are vaccine associated. RT-nested PCR was performed in the small hydrophobic gene region, and viruses were differentiated by restriction fragment length polymorphism assay. A total of 584 nucleotides were amplified. The PCR product of the Hoshino strain was cut into two fragments (313 and 271 nucleotides) by MfeI; that of the Torii strain was digested with EcoT22I, resulting in 332- and 252-nucleotide fragments. Both strains were genotype B and had an XbaI site, resulting in two fragments: 299 and 285 nucleotides. Current circulating wild types were cut only by XbaI or MfeI. However, the MfeI site of the wild types was different from that of the Hoshino strain, resulting in 451- and 133-nucleotide fragments. Using three restriction enzymes, two mumps vaccine strains were distinguished from wild types, and this separation was applied to the identification of vaccine-related adverse events.     

三组中药对耐药菌株的联合作用研究

 目的探讨中药对耐药菌株的联合抑菌作用。方法采用药基法测定了黄芩、大黄、蒲公英、大青叶、鸡屎藤、柳叶白前乙醇提取物对耐药菌株的抑制作用,用棋盘试验法评价了黄芩与大黄、蒲公英与大青叶、鸡屎藤与柳叶白前对耐药菌株的联合作用,用沙门氏菌耐药株人工感染小鼠,观察了鸡屎藤与柳叶白前配伍的体内抗感染效果。结果黄芩、大黄对金黄色葡萄球菌标准株与耐药株的MIC相同,对其他几株耐药菌有较好的抑制作用,蒲公英、大青叶、鸡屎藤、柳叶白前作用较弱,黄芩与大黄存在部分协同或相加作用,蒲公英与大青叶为拮抗作用,鸡屎藤与柳叶白前为部分协同作用并具有较好的体内抗感染作用。结论黄芩与大黄配伍、鸡屎藤与柳叶白前配伍对耐药菌株有较好的抑制作用。