层粘连蛋白、DNA含量及细胞核面积对肺癌患者预后的影响

 采用免疫组化染色，流式细胞术及体视学测量技术对38例肺癌标本进行LN表达、DNA含量及核面积均值测定，用多因素生存分析法分析这三种指标对肺癌患者生存期的影响。结果提示：多因素生存分析表明DI及核面积均值可作为肺癌患者的预后判断指标。     

IL-21 promotes allergic airway inflammation by driving apoptosis of FoxP3+ regulatory T cells

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Further chemical characterization of guinea pig Ia molecules derived from the three major classes of immunocompetent cells.

The guinea pig system is unique in that Ia molecules are readily demonstrable on the three major classes of immunocompetent cells. We were, therefore, able to characterize and compare the structures of Ia molecules from B and T lymphocytes and macrophages. T lymphocyte Ia molecules which bound to Lens culinaris (lentil) lectin co-electrophoresed on SDS-PAGE with lymph node cell Ia molecules (predominantly B lymphocyte Ia molecules) which also bound to lentil lectin. T lymphocyte and macrophage Ia molecules which did not bind to lentil lectin had a slower mobility on SDS-PAGE than lymph node cell Ia molecules which did bind to lentil lectin. One dimensional isoelectric focussing of Ia molecules derived from lymph nodes, T lymphocytes, or macrophages revealed discrete banding patterns for all Ia molecules examined. This result indicates that the Ia molecules which can be chemically isolated from B cells, T cells and macrophages do not have variable regions and probably do not possess antigen recognition capability analogous to immunoglobulin.     

两种细胞外基质分子在慢性肾衰中的变化

目的 :检测慢性肾衰 (CRF)患者血清透明质酸 (HA)及层粘蛋白 (LN)的含量 (x±s,μg L) ,并探讨它们在CRF病理过程中的作用。方法 :用放射免疫法检测 31例CRF患者及 4 0例健康人血清HA及LN浓度 ,统计分析用t检验。结果 :CRF血清HA和LN均比正常对照组显著升高 (P <0 0 1)。其中HA在少尿或无尿期CRF中高于正常对照 5～ 10倍。 11例CRF患者经血透析治疗后血清HA与LN均明显下降 (P <0 0 1)。结论 :HA和LN是构成ECM的重要成分 ,其含量异常增多是致ECM分子过度沉积 ,进而导致肾纤维化及肾功衰竭的因素之一。血液透析治疗可清除或减少ECM的过度蓄积并有助于改善肾功能 ,提示可通过抗ECM或刺激基质分解等途径 ,给CRF患者的治疗带来新的希望     

健肾片对IgA肾病模型大鼠肾组织LNFN局部沉积的影响

目的 :探讨健肾片治疗IgA肾病的机理。方法 :采用口服牛血清白蛋白和葡萄球菌肠毒素B感染的复合方法 ,复制大鼠IgA肾病模型 ,并分别给予健肾片和雷公藤多甙片治疗 ,根据染色程度半定量分析各组肾组织内LN、FN的含量。结果 :健肾片对IgA肾病模型肾组织中LN、FN的沉积具有明显的抑制作用。结论 :健肾片可明显抑制IgA模型肾组织中LN、FN的沉积可能是其治疗IgA肾病的机理之一     

Effect of Annealing Temperature on the Microstructure and Mechanical Properties of High-Pressure Torsion-Produced 316LN Stainless Steel

316LN stainless steel is a prospective structural material for the nuclear and medical instruments industries. Severe plastic deformation (SPD) combined with annealing possesses have been used to create materials with excellent mechanical properties. In the present work, a series of ultrafine-grained (UFG) 316LN steels were produced by high-pressure torsion (HPT) and a subsequent annealing process. The effects of annealing temperature on grain recrystallization and precipitation were investigated. Recrystallized UFG 316LN steels can be achieved after annealing at high temperature. The σ phase generates, at grain boundaries, at an annealing temperature range of 750–850 °C. The dislocations induced by recrystallized grain boundaries and strain-induced nanotwins are beneficial for enhancing ductility. Moreover, microcracks are easy to nucleate at the σ phase and the γ-austenite interface, causing unexpected rapid fractures.     

MNU诱导大鼠膀胱癌基底膜病变与LN的相关性

目的观察N-甲基亚硝基脲(MNU)在诱导SD雌性大鼠膀胱癌的过程中,膀胱基底膜的病变情况与层粘连蛋白(Laminin,LN)的相关性。方法MNU 2 mg/次进行大鼠膀胱灌注,间隔2周,共4次;然后继续正常喂养6周,14周为诱癌终点。分别在MNU灌注后1周及诱癌终点14周用病理学检测观察膀胱粘膜变化情况;免疫组织化学检测基底膜中LN的表达情况,分析其相关性。结果病理结果显示:MNU灌注后膀胱粘膜经过了炎症性改变、异型增生、原位癌、浸润性癌等过程;免疫组化显示基底膜连续、完整的结构,出现断裂、缺损,最后视野下为散在碎片,无基底膜形态;LN在MNU诱导大鼠膀胱癌形成过程中随着基底膜的破坏逐渐减少,缺失;LN在基底膜中的表达在正常膀胱组织与膀胱癌之间存在差异性统计意义(χ2=25.37;P<0.01)。结论膀胱癌的发生、发展与基底膜的破坏密切相关,且LN的缺失是基底膜破坏导致膀胱癌的因素之一。     

Efficacy of the intralesional treatment with Chenopodium ambrosioides in the murine infection by Leishmania amazonensis

AIM OF THE STUDY: Leishmaniasis, caused by protozoan from Leishmania genus, is an endemic disease in the tropical and subtropical regions of the world. The chemotherapy to this disease is not always effective and can cause several side effects. Chenopodium ambrosioides L. (Chenopodiaceae) is used by the native people in the treatment of cutaneous ulcers caused by different species of Leishmania. The aim of this study was to investigate the effect of the treatment with a hydroalcoholic crude extract (HCE) from the leaves of Chenopodium ambrosioides on the murine infection with Leishmania amazonensis. MATERIAL AND METHODS: The mice were treated for 4-6 weeks post-infection (p.i.) with HCE (5 mg/kg) or meglumine antimoniate (Sb(v)) (28 mg/kg) either by the oral route, once a day, for 15 days or by five intralesional (IL) injections at intervals of 4 days. The thickness of the infected paws was determined weekly and the parasite load evaluated in the draining lymph nodes (LN), the spleen and in the footpad after 7 weeks of infection. The nitric oxide (NO) production was evaluated in cultures with cells from peritoneum or LN. RESULTS: The IL treatment increased the NO production in the LN and peritoneum cultures and reduced the parasite load from the footpad, spleen and LN. On the other hand, the oral treatment decreased did alter neither the NO production nor the parasite load. CONCLUSIONS: IL HCE treatment was more efficient than the oral HCE treatment since the former was able to control the dissemination of infection. This effect can be due to either a direct leishmanicidal effect of HCE or the improvement in the NO production by HCE-stimulated macrophages. The results could justify the topical use of the Chenopodium ambrosioides' leaves in the treatment of the ulcers caused by Leishmania.