蝉花菌丝对TGF—β1作用下的肾小管上皮细胞α—SMA等蛋白及mRNA表达的影响

目的：探讨蝉花茵丝对TGF—βl作用下肾小管上皮细胞d—SMA、FN、LN、ColⅢ蛋白及mRNA表达的影响。方法：以TGF—β1,刺激下的肾小管上皮细胞为研究对象,采用血清药理学研究方法和免疫组化、RT—PCR检测技术,观察空白对照组、TGF－β1,对照组、冬虫夏草组、蝉花高剂量组、蝉花中剂量组和蝉花低荆量组的a—SMA、FiN、LN、ColⅢ蛋白及mR－NA表达,采用SPSS11．5统计软件统计分析。结果：TGF-β1,刺激下的肾小管上皮细胞a—SMA、FN、LN、colⅢ蛋白及mRNA的表达与正常组比较明显增加（P〈0．01）;蝉花大、中、小剂量组均能下调肾小管上皮细胞对α－SMA、LN、FN、ColⅢ蛋白及mRNA的表达,与TGF—β1,对照组比较差异有统计学意义（P〈0．05—0．01）,并且大剂量的蝉花茵丝组优于虫草菌丝组（P〈0．05）;不同剂量的蝉花茵丝组存在量效依赖关系。结论：本研究表明蝉花茵丝通过下调TGF－β1,刺激下的肾小管上皮细胞对仪一SMA、LN、FN、ColIⅢ蛋白及mRNA的异常表达而发挥抗肾间质纤维化的药理作用。     

The impact of an N1 lymph node examination in patients with early-stage non-small cell lung cancer: a retrospective cohort study

BackgroundThe examination of lymph nodes (LNs) is critical for accurate node staging in patients with non-small cell lung cancer (NSCLC), but a consensus on the examinations of hilar and intrapulmonary (N1 station) LNs has not been reached. This study aimed to evaluate the role of LN dissection and pathological examination of N1 LN stations and their effects on survival in patients with stage IA-IIA NSCLC.MethodsData from patients pathologically staged as IA-IIA who underwent radical surgery and confirmed as lacking LN metastases from January 2008 to March 2018 were retrospectively reviewed. The Kaplan-Meier method was used to determine the overall survival (OS) and disease-free survival (DFS). After propensity score matching (PSM), a Cox model was used to determine the prognostic factors.ResultsOf the 1,935 patients investigated, the median number of N1 stations examined was 3. Patients with at least 2 N1 stations examined had apparently better OS (P=0.002) and DFS (P=0.001). All patients were divided into patients with 0–1 N1 station examined and patients with 2–5 N1 stations examined. After PSM, the number of N1 stations examined was an independent prognostic factor for DFS (P=0.004). Patients with 2–5 N1 stations examined experienced prolonged DFS (P=0.010). Patients in group 12 experienced prolonged OS (P=0.021) and DFS (P=0.026). Patients in group 13 or 14 experienced prolonged OS (P=0.028).ConclusionsA larger extent of N1 station examination was associated with prolonged DFS in patients with stage IA-IIA NSCLC after lobectomy. The dissection and examination of at least 2 N1 stations included LNs from the lobar and segmental drainage fields.     

LN和MMP-9表达与喉癌的相关性研究

目的 研究层粘连蛋白(laminin,LN)和基质金属蛋白酶-9(MMP-9)在喉癌组织中的表达与其生物学行为关系以及它们之间的相关性.方法 采用免疫组织化学SP法检测46例喉癌及30例正常喉咽黏膜中LN和MMP-9的表达,分析两者在有无淋巴结转移的癌组织中表达的差异及其相关性.结果 LN和MMP-9在喉癌组织的阳性表达率(分别为87.2%和91.5%)明显高于正常喉组织(分别为9.1%和18.2%);LN和MMP-9在喉癌组织的阳性表达与有无淋巴结转移情况有关;LN和MMP-9的表达呈正相关性.结论 LN和MMP-9在喉癌组织有阳性表达,表达的强度与喉癌的不同分化程度、临床分期、有无淋巴结转移情况有关,LN和MMP-9在喉癌中的表达有明显的相关性,表明两者可能在喉的发生发展及淋巴结转移过程中起协同作用.     

血清肝纤维化指标在慢性肝病中的临床分析

目的：探讨联合测定血清透明质酸（HA）、层黏蛋白（LN）、Ⅲ型前胶原（PCⅢ）及Ⅳ型胶原（Ⅳ-C）对慢性肝炎肝纤维化的诊断意义。方法：采用放射免疫分析法检测143例肝病患者的HA、LN、PCⅢ、Ⅳ-C,并与30例健康体检者对比。结果：各组肝病四项纤维化指标较对照组有明显升高。结论：联合测定HA、LN、PCⅢ及Ⅳ-C对肝纤维化的诊断有较高的临床价值,并适合于动态观察肝病的进展,对肝病的预后有一定的指导意义。     

人黑色素瘤细胞系基因突变的研究

目的　揭示金属蛋白酶类 (MMPs) ,层粘连蛋白受体 (LN R)与人黑色素瘤细胞侵袭转移的关系 ,并探讨MMPs ,LN R用以判断肿瘤细胞侵袭转移的可能性。方法　通过流式细胞术 (FCM )定量研究和蛋白酶活性分析 (zy mography) ,对具有不同潜在转移能力的人黑色素瘤细胞进行研究。 结果　早期WM 3 5不产生MMPs ;WM 13 4 1B仅产生MMP 2不产生MMP 9;进展期WM 983A和远处转移瘤株WM 45 1既产生MMP 2又产生MMP 9。瘤细胞表面67KDLN R的荧光阳性率和全部细胞的平均荧光强度大小顺序为WM 45 1>WM 983A >WM 13 4 1B >WM 3 5。结论　MMPs和LN R与人黑色素瘤细胞系的侵袭转移能力获得之间存在着密切关系 ,并可作为一种较特异的肿瘤侵袭转移标记物被应用于肿瘤研究与治疗中。     

The Mayo Clinic Histiocytosis Working Group Consensus Statement for the Diagnosis and Evaluation of Adult Patients With Histiocytic Neoplasms: Erdheim-Chester Disease,Langerhans Cell Histiocytosis,and Rosai-Dorfman Disease

Histiocytic neoplasms, a rare and heterogeneous group of disorders, primarily include Erdheim-Chester disease, Langerhans cell histiocytosis, and Rosai-Dorfman disease. Due to their diverse clinical manifestations, the greatest challenge posed by these neoplasms is the establishment of a diagnosis, which often leads to a delay in institution of appropriate therapy. Recent insights into their genomic architecture demonstrating mitogen-activated protein kinase/extracellular signal–regulated kinase pathway mutations have now enabled potential treatment with targeted therapies in most patients. This consensus statement represents a joint document from a multidisciplinary group of physicians at Mayo Clinic who specialize in the management of adult histiocytic neoplasms. It consists of evidence- and consensus-based recommendations on when to suspect these neoplasms and what tests to order for the diagnosis and initial evaluation. In addition, it also describes the histopathologic and individual organ manifestations of these neoplasms to help the clinicians in identifying their key features. With uniform guidelines that aid in identifying these neoplasms, we hope to improve the awareness that may lead to their timely and correct diagnosis.     

A new benzothiadiazine derivative,LN 5330: effects on pancreatic hormones

Summary LN 5330 is a new benzothiadiazine which is a structural analogue of diazoxide. Its effects in vivo were studied on blood glucose levels and insulin, glucagon and somatostatin secretion in normal dogs, and in vitro on glucagon and insulin secretion from the isolated perfused rat pancreas. The results were compared with those obtained with diazoxide at equimolar dose or concentration. In the normal anaesthetized dog having a T-shaped catheter inserted in the pancreaticoduodenal vein, the infusion of LN 5330 (87.8 mol/kg for 20 min) induced (1) a progressive increase in blood glucose levels, (2) a rapid decrease in insulin and somatostatin output rate, (3) an immediate increase in pancreatic glucagon secretion, and (4) a delayed decrease of arterial blood pressure. The equimolar dose of diazoxide provoked the same effects on blood glucose levels, insulin and somatostatin output, but a marked decrease in glucagon output and in arterial blood pressure. In the isolated rat pancreas perfused with 8.3 mmol/l glucose, the infusion of LN 5330 (440 mol/l for 30 min) induced a drastic fall in insulin and a rapid and persistent increase in glucagon output. This stimulatory effect on glucagon secretion was not found with diazoxide at equimolar concentration. These findings show that LN 5330 is a substance which is distinct from diazoxide and interesting because of its double action: inhibition of insulin secretion and stimulation of glucagon secretion.