川崎病患儿急性期血浆IL-2、IL-4、IL-6、IL-10、IFN—γ、TNF-α水平的变化及意义

【目的】探讨（白细胞介素-2）IL-2、IL-4、IL-6、IL-10、（干扰素-7）IFN-7、（肿瘤坏死因子-a）TNF-“在川崎病（KD）急性期发病中的作用及其与冠状动脉损害（CAL）之间的关系。【方法】采用前瞻性试验方法挑选符合本研究的KD患儿50例、健康儿童65例。采用ELISA法检测两组血清IL-2、IL-4、IL-6、IL-10、IFN-γ、TNF-α的浓度,并将KD组分为CAL组和非CAL组进行对比。【结果】①KD组IL-4、IL-6浓度较健康对照组明显升高,差异有统计学意义（P〈0．05）;但IL-2、IL-10、IFN-7、TNF-α浓度与健康对照组浓度相近,差异无统计学意义（P〉0．05）。②CAL纽IL-6浓度较非CAL组IL-6浓度明显升高,差异有统计学意义（P〈0．05）;两组间IL-2、IL-4、IL-10、IFN-γ、TNF-α浓度相近,差异无统计学意义（P〉0．05）。【结论】IL-4、IL-6水平的升高与KD血管炎症的发生有关,KD急性期监测IL-4、IL-6水平的变化,对于判断KD病情变化及转归有帮助。     

不同材料PFM对患者牙周组织、龈沟液中炎症因子水平的影响

[目的]探讨不同材料的烤瓷熔附金属全冠(PFM)修复后对患者牙周组织、龈沟液中炎症因子水平的影响.[方法]选取本院2013年1月至2015年6月接受牙修复的患者60例,根据修复材料分为:镍铬合金烤瓷冠修复(A组)、镍铬合金镀金烤瓷冠修复(B组)、金沉积烤瓷冠修复(C组)各20例,测定三组患者修复前、修复后6个月的龈沟液量(GCF)、龈沟探诊深度(GCD)、牙龈指数(GI)、龈沟液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)的水平.[结果]修复前,A组、B组和C组患者的GCF、GCD、GI指数差异均无统计学意义(P＞0.05);修复6个月后复查,A组患者的GCF、GCD、GI指数较修复前显著升高(P＜0.05),A组患者的GCF、GCD、GI指数显著高于B组和C组患者(P＜0.05);修复前,A组、B组和C组患者龈沟液中TNF-α、IL-1β、IL-6、IL-8的水平差异均无统计学意义(P＞0.05);修复6个月后复查,A组患者的TNF-α、IL-1β、IL-8的水平较修复前显著升高(P＜0.05),A组患者的TNF-α、IL-1β、IL-8水平显著高于B组和C组患者(P＜0.05).[结论]镍铬合金烤瓷冠对患者的牙周组织影响更为明显,患者牙龈沟液中炎症反应因子水平更高.     

老年慢性气管炎患者血清及痰液白细胞介素水平的检测及其意义

目的 :探讨老年慢性支气管炎患者血清及痰液白细胞介素水平变化及其意义。方法 :以放射免疫法测定 42例老年慢性支气管炎急性发作患者 ,3 7例老年慢性支气管炎缓解期患者以及 3 1例健康对照者血清及痰液中IL 2、IL 4、IL 6、IL 8水平 ,并对以上三组上述细胞因子水平进行统计学分析。结果 :急性组与缓解组血清与痰液中IL 4、IL 6、IL 8水平均高于正常对照组 (P <0 0 5 ) ,其中 ,急性组血清与痰液中上述细胞因子水平较对照组更高(P <0 0 5 )。但是 ,急性组与缓解组IL 2水平低于正常对照组 (P <0 0 5 ) ,其中 ,急性组较缓解组更低 (P <0 0 5 )。同时 ,直接相关分析表明 ,急性组血清IL 6与IL 8、IL 6与IL 4呈正相关关系 (P <0 0 5 ) ;痰液中IL 4、IL 6、IL 8相互间呈正相关关系 (P <0 0 5 )。结论 :IL 2、IL 4、IL 6、IL 8均参与老年慢性支气管炎的发病机制 ,在慢性气道炎症病理过程及急性发作中起着重要作用。这些指标的测定对于老年慢性支气管炎的诊断及炎症反应评估有一定的临床价值。     

肝硬化患者血清IL-6、TNF和尿液IL-6、IL-8检测的临床意义

目的 探讨细胞因子在肝硬化发病中的作用。方法 采用双抗体夹心Elisa法对54例肝硬化患和35例正常人血清白细胞介素－6（IL-6)、肿瘤坏死因子（TNF）和尿液IL－6、IL－8进行检测。结果 肝硬化患血清中IL－6、TNF和尿液IL－6、IL－8含量较对照组明显升高（P＜0．01）,血IL－6、TNF含量GN与尿液量白蛋白呈高度正相关：尿液IL－6、IL－8呈显正相关（r=0．5728,P＜0．05）。结论 肝硬化患病程中TNF、IL－6、IL－8均处于高活性状态,IL－6与体液免疫反应亢进所致的免疫病理损伤有关,IL－6、IL－8、TNF参与肾脏的免疫损伤、可作为判定患预后和转归指标。     

Tumor-associated lymphocytes (TAL) are competent to produce higher levels of cytokines in neoplastic pleural and peritoneal effusions than those found in sera and are able to release into culture higher levels of IL-2 and IL-6 than those released by PBMC

This work was designed to study the proliferative response of tumor-associated lymphocytes (TAL) from neoplastic effusions against autologous tumor cells and the immunophenotype pattern of TAL from neoplastic effusions and that of PBMC of the same patients. We also compared the serum levels of the cytokines interleukin (IL) 1, 2 and 6, tumor necrosis factor- (TNF) and soluble IL-2 receptor (sIL-2R) with those present in neoplastic effusions of the same patients. Moreover, we examined the ability of TAL and peripheral blood mononuclear cells (PBMC) to produce and release the cytokines and sIL-2R and to express membrane CD25 following their stimulation with phytohemagglutinin (PHA) in vitro. Finally, we compared the cytokines/sIL-2R production and membrane CD25 expression by PHA-stimulated PBMC of the patients with neoplastic effusions with a series of 90 cancer patients without neoplastic effusions and 20 normal healthy subjects. Thirteen neoplastic pleural and eight peritoneal effusions were collected from 11 patients with primary lung cancer, 7 with primary epithelial ovarian cancer, 1 with breast cancer, 1 with pleural mesothelioma, and 1 with pancreatic cancer. The proliferative response of TAL from neoplastic effusions against autologous tumor cells was lower than the response to PHA, IL-2, and anti-CD3, but significant. The percentage distribution of CD3⁺ and CD8⁺ lymphocyte subpopulations was higher in peritoneal than in pleural effusions, while the CD16⁺ subset was higher in pleural than in peritoneal effusions. The percentage distribution of CD16⁺ was significantly lower in pleural effusions than in PBMC of patients with pleural effusions. The CD39 antigen was higher on TAL from peritoneal effusions than on PBMC of the same patients. The levels of IL-1 and sIL-2R in peritoneal effusions did not differ from those measured in the sera of the same patients, while the levels of IL-2, IL-6, and TNF were higher in the peritoneal effusions. The levels of IL-2, IL-6, TNF, and sIL-2R, but not IL-1, in pleural effusions were significantly higher than those found in the sera of the same patients. The amounts of IL-2 and IL-6 produced by TAL were generally higher than those released by PBMC. The secretion of cytokines IL-1, IL-2, and sIL2R by PHA-stimulated PBMC was lower, but IL-1 and IL-6 secretion was higher in cancer patients with neoplastic effusions than in either cancer patients without neoplastic effusions or normal subjects. The CD25 expression on PHA-stimulated PBMC derived from cancer patients with neoplastic effusions was in the same range as that of cancer patients without neoplastic effusions and normal subjects. These findings suggest that TAL may be able to produce cytokines and may be amenable to immune manipulation.Abbreviations FITC Fluorescein-isothiocyanate - IL Interleukin - mAb Monoclonal antibody - MHC Major histocompatibility complex - NK Natural killer - PBMC Peripheral blood mononuclear cells - PHA Phytohemagglutinin - TAL Tumor-associated lymphocytes - TIL Tumor-infiltrating lymphocytes - TNF Tumor necrosis factor- - sIL-2R Soluble interleukin-2 receptor     

加味玉屏风散对慢性阻塞性肺疾病急性加重期患者肺功能和细胞因子的影响

目的观察加味玉屏风散对慢性阻塞性肺疾病(COPD)急性加重期患者肺功能和血清中白细胞介素(IL)-17和IL-8的影响,为加味玉屏风散干预治疗COPD提供理论依据。方法将62例急性COPD患者随机分为加味玉屏风散治疗组(31例)和病例对照组(31例)。对照组给予常规治疗;治疗组在常规治疗基础上加用加味玉屏风散,8周后观察2组临床疗效以及治疗前后的肺功能第1秒用力呼气量占预计值百分比(FEV1%)、总气道阻力(R5)和共振频率变化;采用酶联免疫吸附试验法(ELISA)测定血清IL-8I、L-17的水平。结果①加味玉屏风散治疗组的总有效率(87%)较对照组总有效率(68%)升高,且差异有统计学意义(P<0.05)。②2组的FEV1%经过治疗后,均较治疗前升高,差异有统计学意义(P<0.05);治疗组较对照组FEV1%升高,且差异有统计学意义(P<0.05);R5、共振频率2组治疗后较治疗前明显下降,且治疗组较病例对照组下降,差异有统计学意义(P<0.05)。③2组血清IL-17I、L-8水平治疗后均较治疗前明显下降(P<0.05);治疗后治疗组较对照组有明显下降,差异有统计学意义(P<0.05)。结论中药玉屏风散对COPD患者可下调IL-17I、L-8水平,并改善患者肺功能。     

Systemic liberation of interleukin-1β and interleukin-1 receptor antagonist in the perioperative phase of liver transplantation

We measured systemic serum levels of interleukin-1 receptor antagonist (IL-1ra), interleukin-1 (IL-1), tumor necrosis factor (TNF-), and interleukin-6 (IL-6) during the preoperative, anhepatic, and postreperfusional phases up to the 7th postoperative day in 60 patients undergoing orthotopic liver transplantation (LTx). In contrast to IL-1, IL-1ra, TNF-, and IL-6 showed a significant elevation in relation to the early phase after reperfusion, while TNF- displayed a high grade of scatter. In addition, IL-1ra levels were significantly elevated during the anhepatic phase. Maximum serum levels were found at 15 min after reperfusion, 120 min after reperfusion, and on the 1st postoperative day, respectively. Serum levels decreased considerably at 24 h and 7 days after reperfusion. The comparative monitoring of systemic cytokine and cytokine antagonist levels, in particular the liberation of IL-1ra and IL-6 may provide useful parameters for the development of new liver preservation theories for LTx.     

两种麻醉方法对胃癌手术患者血清T淋巴细胞亚群及炎性因子水平的影响

目的比较全身麻醉与全身麻醉复合硬膜外腔阻滞麻醉对胃癌手术患者血清T淋巴细胞亚群及白细胞介素-6（IL-6）、白细胞介素-10（IL-10）、肿瘤坏死因子-α（TNF-α）水平的影响。方法选择60例胃癌手术患者,按数字表法随机分为I组和Ⅱ组各30例,I组采用全身麻醉方法,Ⅱ组采用全身麻醉复合硬膜外腔阻滞麻醉方法,分别于麻醉前、麻醉后2h、术后1d和术后6d检测两组血清CD3＋、CD4＋、CD4＋／CD8＋及IL-6、IL-10、TNF-α的水平,并进行比较。结果两组麻醉后2h、术后1d血清CD3＋、CD4＋、CD4＋／CD8＋水平均较麻醉前明显下降（均P〈0．05）,且I组下降水平大于Ⅱ组（P〈0.05）;两组麻醉后2h血清IL-6水平均明显升高（均P〈0．05）,两组IL-10、TNF-α水平无明显变化（均P〉0．05）。术后6d两组各项观察指标均恢复至术前水平。结论全身麻醉复合硬膜外腔阻滞麻醉对胃癌手术患者术后免疫功能有一过性抑制作用,且抑制程度轻于全麻身麻醉方法,而对患者的炎性反应影响较小。     

Pustulosis palmoplantar: características epidemiológicas,clínicas y genéticas de una serie de 41 casos

Introduction

Palmoplantar pustulosis (PPP) is characterised by the presence of sterile association with other diseases differ among published studies. IL36RN gene mutation has recently been identified in patients with mainly generalised pustular forms, but also in some patients with PPP. The objective of this study is to describe clinical and epidemiological characteristics, the incidence of inflammatory diseases in PPP, and to study the frequency of IL36RN gene mutations.

异丙酚和异氟醚对食管癌手术患者围术期细胞因子平衡的影响

目的研究异丙酚和异氟醚对开胸食管癌手术患者围术期炎性与抗炎性细胞因子平衡的影响.方法择期食管癌手术患者25例,随机分为两组.异氟醚组平均呼末浓度为0.6%,异丙酚组微泵输入剂量为6mg@kg-1@h-1.监测麻醉前、手术开始切皮时、进胸后2h、鼓肺后60min、术后1h、4h、24h血清IL-6、IL-8、IL-10的浓度.观察围术期血液动力学变化及术后临床征象.结果两组进胸后各时点IL-6、IL-8、IL-10的浓度均较术前显著增加(P＜0.01),炎性与抗炎性细胞因子分别于术后1h、4h达到高峰.IL-6组问比较无显著差异,异氟醚组膨肺后60min至术后4h各时点IL-8增加的幅度明显高于异丙酚组(P＜0.05),但术后24h两组无差别.异丙酚组术后1h、4h、24h IL-10的产生均显著高于异氟醚组(P＜0.05或P＜0.01).结论临床剂量的异丙酚促进IL-10的产生,而异氟醚促进了IL-8的分泌,但麻醉选择对于细胞因子平衡的干预尚未影响到机体的免疫自稳.