丝素固定化凝血酶的工艺研究

目的:考察家蚕丝素对凝血酶的固定化作用。方法:以家蚕丝素为载体,戊二醛为交联剂制备固定化凝血酶。以固定化凝血酶的活力回收率（AR）为考察指标,采用正交试验确定丝素固定化凝血酶的最佳工艺条件。结果:丝素固定化凝血酶的最佳工艺条件为:固定时间为6 h,加酶量为2 400 NIH·g^-1载体,温度为25℃,p H为7.6。固定化凝血酶的回收率（AR）达67.22%。结论:丝素对凝血酶有良好的固定化作用。     

Intermittent active motion versus immobilization in the treatment of Staphylococcus aureus-induced arthritis in a rabbit model

Purpose  This study has evaluated the effects of immobilization versus intermittent active motion on cartilage and on antibiotic efficacy in a rabbit septic arthritis model. Methods  Rabbits were infected and assigned to one of four groups: group 1, no treatment without immobilization (allowing intermittent active motion); group 2, cast; group 3, oxacillin without immobilization; group 4, oxacillin and cast. Animals were sacrificed 21 days later. Bacterial counts and lateral radiograms were performed. A radiological score was calculated. Results  Immobilization had no effect on oxacillin efficacy and a deleterious effect on the radiological score. Conclusion  Intermittent active motion has allowed a better cartilage healing during the treatment of septic arthritis.     

活动性并指固定治疗手部掌指关节缺损

目的 报道利用伤指邻近的正常手指作为生理性外固定支架与伤指并指固定,应用带血供的掌骨近端骨块修复２￣５掌指关节缺损的效果。方法 以掌背动脉为轴心,设计逆行掌骨近端关节面修复掌指关节缺损,用１或２枚克氏针将伤指与相邻正常手提横行贯穿固定,保持各关节的自由活动,术后早期功能锻炼,７周左右拆除克氏针。 结果 治疗２￣５掌指关节缺损１１例获得成功,修复后的掌指关节对位对线好,关节间隙存在,术后１￣３个月关     

Synthesis and characterization of both ionically and enzymatically cross-linkable alginate

Alginate with phenol moieties in the polymer side chains was synthesized through the conjugation reaction of alginate and tyramine. Immersing an aqueous solution of the alginate containing horseradish peroxidase into a solution containing H(2)O(2) caused the solution to gel via peroxidase-catalyzed oxidative coupling of the phenols. In addition, alginate prepared under appropriate reaction conditions retained the attractive properties associated with unmodified alginate; spherical gel beads were formed by dropping an aqueous alginate solution (1.0wt.%) into a solution containing calcium ions. The oxidative coupling of the phenols was effective for suppressing the destabilization of the alginate gel resulting from a loss of bonding between the divalent cations and alginate. The mechanical properties of the resultant gels were influenced by the preparation conditions of the alginate and the type of cross-linking.     

固相基质表面von Willebrand Factor抗体的固定化研究

以丝素蛋白膜和聚乳酸膜为基质,采用辉光放电等离子体处理技术和共价交联的方法对vonWillebrand factor(vWf)抗体进行了固定化研究。利用抗体过剩法和酶联免疫实验对固定化效率进行了评价,固定化抗体的活性采用体外凝血时间(APTT,TT和PT)测定进行检测。结果显示固定化效率最高可以达到23.88%,酶联免疫实验均显示阳性,APTT、TT项有明显延长,有的还超出了仪器的测量范围,由此说明,通过这种方法可以有效地对vWf抗体进行共价固定化。本研究拓宽了抗体固定化技术的应用范围,为血管性假血友病的早期快速诊断仪器或试剂盒的研制打下技术基础,同时为其它凝血因子抗体相关的生物材料研究与开发提供一种新思路。     

自酸蚀粘接技术在外伤牙固定中的应用

为了提高外伤牙的治疗有效率, 作者采用自酸蚀粘接剂-扁钢丝-树脂共同构成粘接性夹板,用于固定外伤后松动或脱位的患牙,取得了良好的效果.     

Insufficient Evidence for Routine Use of Thromboprophylaxis in Ambulatory Patients with an Isolated Lower Leg Injury Requiring Immobilization: Results of a Meta-Analysis

Abstract Background:   There are no generally accepted guidelines for the prevention of venous thromboembolism (VTE) in ambulatory patients requiring immobilization after an isolated lower leg injury. Our objective was to evaluate the effectiveness and safety of pharmacological interventions for preventing VTE in these patients. Study Design:   Meta-analysis of randomized controlled trials. Materials and Methods:   We searched PubMed/Medline, EMBASE and the Cochrane Central Register of Controlled Trials for trials with random allocation of thromboprophylaxis, notably low molecular weight heparin (LMWH) versus no prophylaxis or placebo, in ambulatory patients with below-knee or lower leg (including the knee joint) immobilization. Outcome was analyzed using MIX to calculate the pooled risk ratio/relative risk (RR) for each outcome, along with its 95% confidence interval (CI). Results:   The RR of asymptomatic deep vein thrombosis (DVT) was 0.66 (95% CI 0.44; 1.02) for below-knee immobilization and 0.51 (95% CI 0.37; 0.70) for lower leg immobilization. Low molecular weight heparin versus no prophylaxis or placebo was evaluated. The incidence of symptomatic DVT and PE was too low to show any statistically significant difference between thromboprophylaxis and controls in both groups. Although only one adverse bleeding event was considered to bemajor, the RR for any adverse bleeding event was 1.94 (95% CI 1.03; 3.67). Conclusion:   There is insufficient evidence to warrant routine use of thromboprophylaxis in ambulatory patients with below-knee or lower leg immobilization after an isolated lower leg injury. The incidence of symptomatic VTE is too low to show a relevant clinical benefit from thromboprophylaxis.