NaFeEDTA预防大鼠铅中毒的研究及其对锌、铜的影响

为探讨乙二胺四乙酸铁钠 (NaFeEDTA)在预防大鼠铅中毒及减低铅毒性中的作用 ,同时研究其对锌、铜二元素的影响 ,采用随机分组的动物实验设计方法 ,将 60只Wistar大鼠随机分为空白对照组、模型对照组、阳性药物组和高、中、低NaFeEDTA组 ,共 6组 ,除空白对照组外 ,对其余各组大鼠进行持续性染铅 ,而且除模型对照组外的 4个染铅组分别同时给予二巯基丁二酸 (DMSA)、高、中、低浓度的NaFeEDTA溶液。 5周后实验结束 ,对相关指标进行测定。结果显示 ,三个NaFeEDTA组的血红蛋白含量均显著高于其它各组 ,同时其血铅、脑铅、肝脏铅、肾脏铅及胫骨铅含量显著低于模型对照组 ,而且它们的肝脏铅、肾脏铅和胫骨铅含量与每日每只大鼠铁补充量之间存在显著负相关 ,相关系数分别为 - 0 4432、- 0 61 34和 - 0 3878;除中、高浓度NaFeEDTA组的脑锌水平低于模型对照组以及低浓度NaFeEDTA组的肾脏铜含量高于模型对照组外 ,其余各NaFeEDTA组的脏器锌、铜含量与模型对照组相比均无显著性差异。提示NaFeEDTA可有效提高大鼠的血红蛋白含量 ,能够预防大鼠铅中毒 ,减低铅对大鼠的毒性作用 ;同时对体内锌、铜含量无太大影响     

阿魏酸在血瘀证兔体内的药代动力学

为验证及研究症治药动学假说（ｓｙｎｄｒｏｍｅａｎｄＴｒｅａｔｍｅｎｔＰｈａｒｍａｃｏｋｉｎｅｔｉｃｓ，Ｓ＆ＴＰＫ）的客观性和基本规律，本文以ＲＰ－ＨＰＬＣ法同时测定了活血祛瘀药物阿魏酸在正常及高分子右旋糖酐所制血瘀症（微循环障碍）兔体内经时浓度，以ＭＣＰＫＰ药动学程序在ＣＯＭＰＡＱ８０３８６机上自动拟合了药动学参数。统计结果表明，与正常组相比，阿魏酸在血瘀症兔体内的分布容积（Ｖ＿１和Ｖ＿Ｂ）及消除速率（ＣＬＢ）显著减小（Ｐ＜０．０５），半衰期（ｔ＿（１／２β）及相同时间段曲线下面积（ＡＵＱ）非常显著增加（Ｐ＜０．０１）。结论：实验结果基本符合Ｓ＆ＴＰＫ的观点：即同一药物在不同证兔体内的药代动力学参数经统计学处理有显著差异。     

复合膳食纤维对大鼠体内脂质过氧化作用的影响

制备复合膳食纤维 (dietaryfiber,DF) ,并分别探讨复合、混合及三种单一的DF对高脂血症大鼠体内脂质过氧化作用的影响。选健康、断乳Wistar大鼠 6 4只 ,按体重随机分为 8组 ,用高脂饲料诱发高脂血症的同时 ,分别添加 10 %的DF :纤维素 (B组 )、果胶 (C组 )、海藻酸钠 (D组 )、纤维素 -果胶复合物 (E组 )、纤维素 -海藻酸钠复合物 (F组 )、纤维素 -果胶混合物 (G组 )、纤维素 -海藻酸钠混合物 (H组 ) ,以单纯的高脂饲料组为对照组 (A组 ) ,观察各种DF对大鼠的生长发育及脂质过氧化作用的影响。结果显示 :1、添加 10 %的各种DF不影响大鼠的生长发育。 2、各种DF皆可显著升高血清超氧化物歧化酶 (SOD) (P <0 0 1)、谷胱甘肽过氧化物酶 (GSH Px)活性 (P <0 0 1) ,降低丙二醛 (MDA)水平 (P <0 0 5 ,B、D组除外 ) ,提高红细胞膜的流动性 (P <0 0 5 ) ,以复合物、混合物效果为明显。 3、各种DF可不同程度地增加粪重和粪脂排出量 (P <0 0 5 ,D组除外 ) ,以复合物、混合物为明显。提示各种DF皆可不同程度地降低大鼠体内脂质过氧化作用 ,提高红细胞膜的流动性 ,其中可溶性DF效果优于不可溶性DF ,而复合、混合DF的效果又优于单一的DF ,且以复合物效果为佳     

Perceptions of the at-risk nonsmoker: are potential intervention topics being overlooked?

This research is supported in part by Arizona Disease Control and Research Commission (ADCRC), Grant #9904 (M. Burgoon, PI). This study assesses how adolescent nonsmokers, at-risk smokers, and smokers differ in perceptions of smoking harms, benefits, and safety. While small differences exist when comparing attitudes and knowledge concerning short- and long-term benefits of smoking, large differences arise when attitudes and knowledge concerning the short-term benefits and the safety of casual smoking are compared.     

The Decline in Serum Albumin after Conversion to High Flux, High Efficiency Dialysis

Abstract: Hypoalbuminemia among chronic hemodialysis patients is recognized as a poor prognostic sign. We observed that many of our chronic patients had a progressive decrease in their plasma albumin concentrations after they were converted to high flux, high efficiency dialysis from conventional dialysis mode. This change occurred in the absence of changes in the KTIV and protein catabolic rate (per) normalized to body mass. When nitrogen losses were measured, we found no difference in the dialysate concentrations of urea, alpha amino nitrogen, uric acid, or total nitrogen when high flux polysulfone was compared with high efficiency Cuprophan. While urea was the predominant nitrogen solute in all dialysate samples, there were some with a large gap between total and urea nitrogen. Alpha amino nitrogen losses, expressed as leucine equivalents, were substantial, ranging from 8. 4 to 9. 8 g/3. 5 h dialysis treatment. We believe that the increased losses of nitrogen experienced by patients after their conversion to a more efficient method of dialysis and not compensated for by a spontaneous increased intake of protein led to the observed fall in plasma albumin. Both urea and amino acid nitrogen losses need to be accounted for when achievement of higher KTIV dialysis is pursued.     

hNa_v1.8mRNA在三叉神经痛患者痛支神经中的表达

目的：观察河脉毒素不敏感型钠通道Navl．8 mRNA在三叉神经痛(trigeminal neuralgia,TN)患者痛支神经中的表达。方法：利用RT—PCR技术，以β—actin为内参照，检测5例原发性TN保守治疗无效患者的痛支神经、2例行舌颌颈联合根治术患者正常耳大神经和肌肉组织中hNavl．8 mRNA的表达。结泉：hNavl．8 mRNA在TN患者痛支神经中有明显表达，而在正常耳大神经及肌肉组织中均无表达。结论：hNavl．8 mRNA在三叉神经痛支中的异常表达可能与TN的发病机制有关。     

移植肾破裂的处理

目的　提高移植肾破裂的防治水平。方法　 6例移植肾破裂 ,手术前 2例 ,手术后 4例。 2例术前供肾破裂 ,采用切开移植肾破裂处包膜 +裂口内明胶海绵填塞 +肠线修补 +肠线编织肾袋收缩保护移植肾。 1例术后移植肾破裂早期 ,出血少 ,针对顽固性高血压采用“硝普钠”降压 ,配合常规抗排斥药物。 3例术后移植肾破裂出血量估计超过 10 0 0ml者 ,采用手术延长移植肾破裂处包膜 +裂口内明胶海绵填塞 +肠线修补 +肠线编织肾袋收缩保护移植肾。结果　 ( 1)手术前 2例手术后 4例 ,采用切开或者延长移植肾破裂处包膜 +裂口内明胶海绵填塞 +肠线修补 +肠线编织肾袋收缩保护移植肾并配合“硝普钠”降压的方法处理 ,均未再破裂出血 ,移植肾功能恢复良好。 ( 2 ) 1例术后移植肾破裂早期的患者 ,针对顽固性高血压采用“硝普钠”降压 ,配合常规抗排斥药物 ,非手术治疗成功。结论　 ( 1)采用手术切开或延长移植肾破裂处包膜 +裂口内明胶海绵填塞 +肠线修补 +肠线编织肾袋收缩保护移植肾可以有效治疗移植肾破裂。 ( 2 )移植肾破裂出血少的情况下 ,可以在密切观察下非手术治疗     

透明质酸钠预防粘连性肠梗阻术后再粘连临床观察

Objective To investigate the prevention of intestines adhesion about sodium hyaluronic acid in postoperative intestines adhesion.Methods Eighteen cases of adhesive intestine obstruction were done intestinal release or bowel resection.2～4mL sodium hyaluronic acid was put to the wound,anastigmatic and rough surface of peritoneal.Gastrointestinal decompression,anti-infective and infusion were taken after oper-ations.Followed up 8～24 months.Results Obstructive symptoms haven't happened,the effective rate is 100%.Only 2 cases have intermittent abdominal pain without obstruction,the incidence was 11%.Conclu-sion Sodium hylauronic acid is effective to prevent the adhesion of postoperative intestines adhesion,simp-ler use,fewer side-effects and great value to appliance.     

脊髓前动脉损伤致脊髓缺血性损伤模型的MRI评价

目的：研究经脊髓前动脉损伤，致脊髓缺血性损伤模型的高场强MRI信号表现特征。方法：选用12只家犬经颈前手术入路，切断脊髓前动脉，经高场强MRI扫描，对照病理分析脊髓前动脉损伤后脊髓的MRI信号变化特征。结果：脊髓前动脉损伤后脊髓MRI表现出异常T2WI高信号，且出现在脊髓前动脉损伤节段的前2／3，多表现为一例受累及或偏向一例。结论：MRI能确定脊髓缺血性损伤的部位及信号特征。