全文获取类型
收费全文 | 34474篇 |
免费 | 2484篇 |
国内免费 | 1419篇 |
专业分类
耳鼻咽喉 | 255篇 |
儿科学 | 371篇 |
妇产科学 | 829篇 |
基础医学 | 7710篇 |
口腔科学 | 533篇 |
临床医学 | 2240篇 |
内科学 | 5289篇 |
皮肤病学 | 546篇 |
神经病学 | 1185篇 |
特种医学 | 1426篇 |
外国民族医学 | 4篇 |
外科学 | 1582篇 |
综合类 | 4297篇 |
现状与发展 | 5篇 |
预防医学 | 2596篇 |
眼科学 | 247篇 |
药学 | 3479篇 |
3篇 | |
中国医学 | 779篇 |
肿瘤学 | 5001篇 |
出版年
2024年 | 47篇 |
2023年 | 408篇 |
2022年 | 780篇 |
2021年 | 972篇 |
2020年 | 884篇 |
2019年 | 844篇 |
2018年 | 754篇 |
2017年 | 927篇 |
2016年 | 1066篇 |
2015年 | 1246篇 |
2014年 | 1740篇 |
2013年 | 2307篇 |
2012年 | 1601篇 |
2011年 | 1942篇 |
2010年 | 1581篇 |
2009年 | 1698篇 |
2008年 | 1672篇 |
2007年 | 1730篇 |
2006年 | 1685篇 |
2005年 | 1597篇 |
2004年 | 1455篇 |
2003年 | 1283篇 |
2002年 | 1150篇 |
2001年 | 1033篇 |
2000年 | 917篇 |
1999年 | 747篇 |
1998年 | 721篇 |
1997年 | 644篇 |
1996年 | 595篇 |
1995年 | 568篇 |
1994年 | 596篇 |
1993年 | 418篇 |
1992年 | 435篇 |
1991年 | 327篇 |
1990年 | 308篇 |
1989年 | 252篇 |
1988年 | 224篇 |
1987年 | 142篇 |
1986年 | 156篇 |
1985年 | 200篇 |
1984年 | 164篇 |
1983年 | 66篇 |
1982年 | 85篇 |
1981年 | 94篇 |
1980年 | 91篇 |
1979年 | 66篇 |
1978年 | 56篇 |
1977年 | 34篇 |
1976年 | 35篇 |
1975年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 23 毫秒
151.
S. Doğru-Abbasoğlu G. Aykaç-Toker H. A. Hanagasi H. Gürvit M. Emre M. Uysal 《Neurological sciences》2007,28(1):31-34
Abstract Alzheimer's disease (AD) is defined pathologically by the presence of β-amyloid plaques, neurofibrillary tangles and extensive
neuronal loss. Evidence indicates that increased DNA damage may contribute to neuronal loss in AD. Recently, it has been shown
that in AD neurons have a reduced capacity for some types of DNA repair. Polymorphisms in DNA repair genes may be associated
with differences in repair efficiency of DNA damage. Variants of several DNA repair genes, including the base excision repair
gene XRCC1, have been described previously. We hypothesised that Arg194Trp polymorphism of XRCC1 gene may contribute to genetic susceptibility for AD. In order to test this hypothesis, we investigated
Arg194Trp polymorphism at the XRCC1 gene in the DNA samples of 98 patients with AD and 95 healthy subjects. The frequency of the Trp allele was more pronounced among cases (11.2%) compared with controls (5.8%). On combining the homozygous and heterozygous
variants of each codon, the variants seemed to be at twofold risk of AD, although the risk estimates were not statistically
significant (OR=1.95, 95% CI 0.88–4.34, p=0.09). In addition, the 194Trp allele revealed a borderline significance (OR=2.05, 95% CI 0.96–4.37, p=0.056). According to our results, it may be speculated that the polymorphic variants of XRCC1 codon 194 have a role in the
development of AD. 相似文献
152.
流行性乙型脑炎病毒结构蛋白编码基因的重组构建 总被引:1,自引:1,他引:0
目的:建立流行性乙型脑炎病毒(JEV)C、prME,E等结构蛋白编码基因克隆.方法:病毒感染C6/36细胞,RT-PCR法依次获取该病毒结构蛋白基因并进行DNA测序分析,PCR克隆法将PCR产物分别构建于真核表达载体pcDNA 3.1的BamHI与EcoRI酶切位点并依次命名为pJC、pJME与pJE,通过DNA测序、电泳以及限制性内切酶分析加以鉴定.结果:JEV C、prME与E基因片段分别为380、2001以及1 500bp,各基因测序结果与发表的JEV JaGAr-01株相对应序列相一致,从重组质粒释出的插入子分别与各基因大小相符合.结论:pJC、pJME与pJE重组质粒分别含有C、prME与E蛋白编码基因. 相似文献
153.
目的:探讨PCR检测大鼠外周血及腹水中细菌DNA对空肠-空肠、回肠-回肠吻合口瘘的早期诊断价值。方法:健康Wistar雌性大鼠50只,随机分成5组,每组10只:A组为假手术组;B组为空肠-空肠吻合组;C组为空肠吻合口瘘组;D组为回肠-回肠吻合组;E组为回肠吻合口瘘组。采集手术前后外周血及术后腹水,抽提DNA, 比较lacZ基因和16SrRNA基因的PCR阳性率,并观察各组的病理学情况。结果:(1)C,E组术后外周血lacZ基因PCR阳性率与B,D组无显著性差异(P>0.05);C,E组术后外周血16SrRNA基因PCR阳性率显著高于B,D组(P<0.05)。(2)C,E组腹水lacZ基因和16SrRNA基因PCR阳性率均显著高于B,D组(P<0.05)。(3)C,E组腹水lacZ基因阳性率显著高于外周血(P<0.05);C,E组腹水16SrRNA基因阳性率与外周血无显著性差异(P>0.05)。结论:(1)PCR检测术后外周血16SrRNA基因对空、回肠吻合口瘘的早期诊断有一定意义;(2)检测术后腹水lacZ基因和16SrRNA基因对空肠-空肠、回肠-回肠吻合口瘘的早期诊断也有一定意义。 相似文献
154.
In the evolution of humans, many kinds of mutations in the human genome have been accumulated, providing credible genetic evidence for the study of human origins and migrations. The "out-of-Africa" hypothesis of modern human evolution and the genetic origin of the Japanese has come about by studying mitochondrial DNA.l,2 Recently, researchers have recognized the power of Y-chromosome markers in resolving migratory patterns of modern humans as more and more Y-chromosome single nucleotide polymorphism markers have been found. The markers on the nonrecombinant part of the Y-chromosome allows for the reconstruction of intact haplotypes which are probably the best genetic tools to study human migrations. We can analyze the paternal history of some people in different areas by Y-chromosome haplotypes. 相似文献
155.
156.
157.
Observations on two members of the Swedish family with congenital dyserythropoietic anaemia,type III
S. N. Wickramasinghe A. Wahlin D. Anstee S. F. Parsons G. Stopps I. Bergstrom M. Eriksson H. Sandstrom S. Shiels 《European journal of haematology》1993,50(4):213-221
Abstract: Two affected individuals of the Swedish family with CDA, type III, in which the disease is transmitted as an autosomal dominant character, were studied. Both cases displayed features hitherto undescribed in this family but described in patients with CDA, type III, in whom the inheritance may have been as an autosomal recessive character. Such features were: (a) haemosiderinuria, (b) grossly disorganised erythroblast nuclei, (c) differences in the ultrastructural appearances of individual nuclei within the same multinucleate erythroblast and (d) intraerythroblastic inclusions resembling precipitated globin chains. In both cases the giant mononucleate erythroblasts and the multinucleate erythroblasts had total DNA contents up to 28c (1c = haploid DNA content) and 48c respectively, and some DNA synthesising bi- and multinucleate erythroblasts contained one or more nuclei which were unlabelled with 3H-thymidine. These findings are similar to those in patients with the autosomal recessive type of disease. Thus no major phenotypic differences are yet apparent between cases of CDA, type III, with different patterns of inheritance. Analysis of the surface erythrocyte proteins of the 2 Swedish CDA, type III, patients with monoclonal antibodies recognising Band 3, glycophorins A, B, C and D, Rh, CD44, CD47, CD55, CD58, CD59, Lutheran, Kell, LW and acetylcholinesterase did not reveal any gross abnormality of expression of these proteins. A slightly altered expression of blood group antigens A and H was revealed by the lectins Dolichos biflorus and Ulex europaeus and the Mr of Band 3 as judged by SDS polyacrylamide gel electrophoresis was also slightly reduced, suggesting that there may be minor alterations in the degree of N-glycosylation of some red cell membrane constituents. 相似文献
158.
为探讨流式细胞术对涎腺腺样囊性癌的诊断和预后估计的价值,对41例腺样囊性癌行流式细胞术分析。结果,平均DNA指数为1.179,S期细胞比例为29.49%,细胞增殖指数为42.67%,均明显高于正常组织。异倍体检出率为70.73%。随着肿瘤的增大和恶性程度的增加,其异倍体率、DNA指数和S期细胞比例值也相应增加。流式细胞术为涎腺腺样囊性癌的病理诊断和确定恶性程度提供了有效的辅助诊断措施,但尚不能判断预后。 相似文献
159.
160.
荧光定量聚合酶链反应在尖锐湿疣诊断中的应用 总被引:3,自引:0,他引:3
目的 探讨荧光定量聚合酶链反应 (FQ - PCR)对尖锐湿疣 (CA )的诊断价值。方法 FQ- PCR检测病理确诊的 CA患者 36份标本和健康人 84份标本 ;FQ- PCR及病理诊断同期检测临床送检病例 2 73份标本 ,并做诊断性试验评价。结果 36例病理确诊的 CA患者 HPV6和 HPV11的 DNA FQ- PCR全部阳性 ,平均拷贝数为 1.0× 10 7± 1.0× 10 2 / m l;84例健康人 FQ- PCR全部阴性 ,平均拷贝数为 3× 10± 2× 10 / ml。 FQ- PCR与病理诊断符合率为 10 0 % ,CA患者与健康人拷贝数的差异有显著性 (P<0 .0 5 )。FQ- PCR与病理诊断对比得出 :灵敏度为 10 0 % ,特异度为 92 % ,误诊率为 0 .0 8,漏诊率为 0 ,准确度为 98.9% ,阳性预测值为 98.8% ,阴性预测值为 10 0 % ,阳性似然比为 12 .5 ,阴性似然比为 0。结论 FQ - PCR能准确定量 ,灵敏度高 ,特异性强 ,快速 ,简便 ,可作为尖锐湿疣早期诊断的指标 相似文献