胸腺肽肠溶片联合复方硫酸软骨素治疗类风湿性关节炎的临床研究

目的探讨胸腺肽肠溶片联合复方硫酸软骨素治疗类风湿性关节炎的临床疗效。方法选取2015年5月—2016年5月南阳市骨科医院收治的类风湿性关节炎患者90例,随机分为对照组和治疗组,每组各45例。对照组口服复方硫酸软骨素片,4片/次,3次/d。治疗组在对照组的基础上口服胸腺肽肠溶片,2片/次,3次/d。两组患者治疗时间均为3个月。评价两组患者临床疗效,同时比较治疗前后两组患者临床症状、关节炎相关指标和免疫球蛋白水平。结果治疗后,对照组和治疗组总有效率分别为80.00%和93.33%,两组比较差异具有统计学意义(P0.05)。治疗后,两组肿胀关节数、压痛关节数和晨僵时间均出现明显下降,握力则明显升高,同组比较差异具有统计学意义(P0.05)。且治疗组这些临床症状则明显优于对照组,两组比较差异具有统计学意义(P0.05)。治疗后,两组患者类风湿因子(RF)、血沉(ESR)和C反应蛋白(CRP)水平均显著降低(P0.05)。且治疗组上述关节炎相关指标均明显低于对照组(P0.05)。治疗后,两组患者IgG、IgA及IgM均明显降低(P0.05);且治疗组上述指标含量明显低于对照组(P0.05)。结论胸腺肽肠溶片联合复方硫酸软骨素治疗类风湿性关节炎临床疗效确切,具有一定的临床推广应用价值。     

High susceptibility of human articular cartilage glycosaminoglycan synthesis to changes in inorganic sulfate availability

The effect of sulfate concentration in the medium on glycosaminoglycan synthesis in articular cartilage of five different species was examined in relation to the physiological serum sulfate concentration in these species. Only the rate of sulfated glycosaminoglycan synthesis in human articular cartilage was sensitive to small deviations from the physiological sulfate concentration. A reduction in the sulfate concentration from 0.3 mM (physiological) to 0.2 mM resulted in a 33% reduction in glycosaminoglycan synthesis. In addition, we studied the effect of arthritic and "osteoarthritic" alterations in murine cartilage on the dependence of glycosaminoglycan synthesis on low sulfate concentrations. Arthritic and "osteoarthritic" cartilage had a similar dependence on the sulfate concentration in the medium as normal cartilage. Glycosaminoglycan synthesis in human articular cartilage appears to be very sensitive to the potential sulfate-depleting effects of drugs used in the treatment of rheumatoid arthritis and osteoarthritis.     

间接配合滴定法测定食盐中硫酸根

目的建立食盐中硫酸根的间接络合滴定检验法。方法样品经前处理后加钡镁混合液,在加热条件下,溶液中硫酸根与钡离子反应生成难溶于水的硫酸钡,过量的钡离子在镁离子的置换作用下,以铬黑T为指示剂,以乙二胺四乙酸二钠(EDTA-Na2)滴定,根据钡离子浓度变化计算硫酸根含量。结果加标前后,本方法回收率(96.2%~100.1%)及精密度(0.6%~2.2%)较国家标准方法回收率(85.1%~88.5%)及精密度(1.6%~6.7%)明显提高。结论相比于国家标准方法,本方法以经济实用的氯化镁代替原方法中乙二胺四乙酸二钠镁,节约成本;合并氯化钡与氯化镁为钡-镁混合液,简化操作步骤;添加60℃水浴加热,提升生成硫酸钡的反应效率,提高检测回收率。试剂成本低,操作简便,精密度高,准确性好,更利于推广普及。     

Disease modifying effect of statins in dextran sulfate sodium model of mouse colitis

Objective: We evaluated the disease modifying effect of simvastatin and atorvastatin in Dextran Sulfate Sodium (DSS) model of colitis. Materials and methods: Thirty, 8-week old female Swiss-Webster mice were separated into 5 groups (n = 6/group). Colitis was induced by feeding 4 % DSS solution for 7 days. Following discontinuation of DSS, over the next 7 days, the groups orally received simvastatin (20 mg/kg/day), atorvastatin (60 mg/kg/day), vehicle only (0.75 % methylcellulose), subcutaneous 30 μg injections of anti-TNFα monoclonal antibody or intraperitoneal anti-mouse apolipoprotein A-I antibody respectively. Disease activity Index (DAI) was determined daily by a blinded investigator. Results: The mean reduction in DAI scores from day 7 to day 14 for anti-TNFα group, simvastatin and atorvastatin group were 74 %, 76 % and 64 %, respectively as compared to 41 % reduction in vehicle and anti-apolipoprotein A-I antibodytreated groups. Conclusions: This finding suggests that statins may have the ability to modify the disease activity in the DSS model of colitis and the disease modifying effect is comparable to anti-mouse TNFα treatment in this model. Received 23 October 2006; returned for revision 27 February 2007; accepted by I. Ahnfelt-R?nne 16 August 2007     

角膜塑形镜联合0.01%硫酸阿托品眼用凝胶对小儿近视的疗效及影响因素分析

目的:探讨角膜塑形镜联合0.01%硫酸阿托品眼用凝胶对小儿近视恢复情况的影响及影响患儿疗效的因素。方法:采用前瞻性研究方法,选取2017-03/2019-03就诊于本院的近视儿童200例,根据儿童和监护人要求选择观察组(角膜塑形镜联合0.01%硫酸阿托品眼用凝胶)和对照组(仅配戴角膜塑形镜)。观察组108例纳入研究,6mo后8例舍弃,最终纳入100例完成随访。对照组92例纳入研究,6mo后5例舍弃,最终87例完成随访。收集患者治疗前及治疗后6mo最佳矫正远视力、最佳矫正近视力、裸眼视力、眼压、瞳孔直径和调节幅度及不适症状。结果:观察组患儿治疗后裸眼视力为0.12±0.05,对照组为0.19±0.07,较治疗前均显著改善(P<0.05),且观察组显著优于对照组(P<0.05)。两组治疗后眼压均较治疗前无明显变化(P>0.05);观察组患儿治疗后瞳孔直径为7.01±0.66mm,相比治疗前的6.09±0.69mm显著升高(P<0.05);调节幅度为14.06±4.03D,相比治疗前的15.31±4.40D显著降低(P<0.05),对照组治疗后的瞳孔直径和调节幅度与治疗前比较均无明显变化(P>0.05)。Pearson相关性分析结果表明:患儿近视控制效果与年龄、治疗前裸眼视力和散光度数呈负相关(P<0.05),多因素Logistic回归分析结果显示年龄、治疗前裸眼视力是影响近视控制效果的因素(P<0.05)。结论:角膜塑形镜联合0.01%硫酸阿托品眼用凝胶有助于小儿近视患者视力控制,治疗效果受到患儿年龄、治疗前裸眼视力因素影响。     

Dexmedetomidine and Magnesium Sulfate: A Good Combination Treatment for Acute Lung Injury?

Objectives: In this study, we aimed to investigate the therapeutic effects of magnesium sulfate (MgSO₄) and dexmedetomidine (dex) in a model of acute lung injury (ALI). We determined whether concomitant administration decreased the inflammatory effects of hydrochloric acid (HCl)-induced ALI in a synergistic manner. Materials and Methods: In this study, 42 Sprague–Dawley rats were randomized into six groups: Group S (saline), Group SV (saline + mechanical ventilation), Group HCl (HCl), Group Dex (Dex), Group Mag (MgSO₄), and Group DM (Dex + MgSO₄). All groups except Group S were mechanically ventilated prior to HCl-induced ALI. Saline or HCl was administered via tracheostomy. Prior to treatment, HCl was administered to Group HCl, Group Dex, Group Mag, and Group DM to induce ALI. Dex and MgSO₄ were administered intraperitoneally. The rats were monitored for 4 h after treatment to measure oxidative stress parameters in blood, and prolidase enzyme activity. Lung tissue damage were determined via histopathology. Results: A significant increase in heart rate and rapid desaturation was observed in HCl-administered groups. Treatment administration decreased the pulse values. Increased saturation values and decreased oxidative stress indices were observed in groups that were subsequently administered? Dex and MgSO₄. Serum prolidase activity increased significantly in Group HCl. Severe pathological findings were detected following HCl-induced ALI. Group Mag showed greater improvement in the pathology of HCl-induced ALI than did Group Dex. Administration of both Dex and MgSO₄ did not improve the pathological scores. Conclusions: The antioxidant and anti-inflammatory effects of Dex and MgSO₄ ameliorated the detrimental effects of HCI-induced ALI. However, adverse effects on hemodynamics and lung damage were observed when the two drugs were administered together.     

The toxicity of lead to Desulfovibrio desulfuricans G20 in the presence of goethite and quartz

An aqueous mixture of goethite, quartz, and lead chloride (PbCl₂) was treated with the sulfate‐reducing bacterium, Desulfovibrio desulfuricans G20 (D. desulfuricans G20), in a medium specifically designed to assess metal toxicity. In the presence of 26 μM of soluble Pb, together with the goethite and quartz, D. desulfuricans G20 grew after a lag time of 5 days compared to 2 days in Pb‐, goethite‐, and quartz‐free treatments. In the absence of goethite and quartz, however, with 26 μM soluble Pb, no measurable growth was observed. Results showed that D. desulfuricans G20 first removed Pb from solutions then growth began resulting in black precipitates of Pb and iron sulfides. Transmission electron microscopic analyses of thin sections of D. desulfuricans G20 treated with 10 μM PbCl₂ in goethite‐ and quartz‐free treatment showed the presence of a dense deposit of lead sulfide precipitates both in the periplasm and cytoplasm. However, thin sections of D. desulfuricans G20 treated with goethite, quartz, and PbCl₂ (26 μM soluble Pb) showed the presence of a dense deposit of iron sulfide precipitates both in the periplasm and cytoplasm. Energy‐dispersive X‐ray spectroscopy, selected area electron diffraction patterns, or X‐ray diffraction analyses confirmed the structure of precipitated Pb inside the cell as galena (PbS) in goethite‐ and quartz‐free treatments, and iron sulfides in treatments with goethite, quartz, and PbCl₂. Overall results suggest that even at the same soluble Pb concentration (26 μM), in the presence of goethite and quartz, apparent Pb toxicity to D. desulfuricans G20 decreased significantly. Further, accumulation of lead/iron sulfides inside D. desulfuricans G20 cells depended on the presence of goethite and quartz. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

镁对缺血再灌注心脏内皮细胞功能和血小板活化的影响

为探讨心肌缺血再灌注时内皮细胞功能和血小板活化状态及硫酸镁对它们的影响,将新西兰大耳白兔32只分为4组.对照组在左冠状动脉前降支中上2/3处穿线;缺血组于同一位置持续结扎血管;再灌注组及治疗组结扎0.5 h后再灌注.治疗组于结扎后静滴硫酸镁溶液,其余各组静滴等量生理盐水.于结扎前、缺血0.5 h、再灌注1 h及4 h采血,用火焰原子吸收法测定血浆Mg2+浓度,硝酸还原酶法测定血浆一氧化氮浓度,放射免疫法测定血小板表面α颗粒膜蛋白140.发现①对照组手术前后血浆Mg2+、一氧化氮浓度和血小板表面α颗粒膜蛋白140分子数差别无统计学意义(P>0.05);②缺血组、再灌注组心肌缺血0.5 h,血浆Mg2+、一氧化氮浓度较术前及对照组相应时间显著降低(P<0.05),并随时间延长呈进行性下降,而血小板表面α颗粒膜蛋白140分子数显著升高(P<0.05),并随时间延长进一步上升;③治疗组应用硫酸镁后,血浆Mg2+、一氧化氮浓度较缺血组、再灌注组相应时间明显升高,而血小板表面α颗粒膜蛋白140分子数明显降低,但仍高于对照组(P<0.05).提示缺血再灌注过程中存在内皮细胞功能损伤及血小板活化,硫酸镁可能有保护内皮细胞和抑制血小板活化的作用.     

离子色谱法测定注射用硫代硫酸钠中的亚硫酸盐和硫酸盐

目的 用离子色谱法测定注射用硫代硫酸钠中亚硫酸根和硫酸根的含量.方法 采用IonpacAS11-HC型分离柱(250 mm ×4 mm),淋洗液为20 mmoL·L-氢氧化钾溶液,电导检测法.结果 0.1322～6.6116 μg亚硫酸根、0.0904～4.5174μg硫酸根与色谱峰的峰面积有良好的线性关系,平均回收率分别为98.8％、99.0％.结论 所用方法简便、准确、快速,适用于注射用硫代硫酸钠中亚硫酸根和硫酸根的含量测定.     

Éclampsie

Eclampsia, the major neurological complication of preeclampsia, is defined as a convulsive episode, or any other sign of an altered consciousness, arising in a setting of preeclampsia, and which cannot be attributed to any other preexisting neurological condition.