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101.
生长因子抑制剂—通尿灵治疗前列腺增生症 总被引:2,自引:0,他引:2
目的 为了评价生长因子抑制剂非洲臀果木(Pygeumafricaum)提取物-通尿灵(Tadenan)治疗良性前列腺增生症的疗效。方法40例良性前列腺增生症患者2/d共服用通尿灵100mg,共8周。结果通尿灵可明显改善国际前列腺症状评分(IPSS)、最大尿流率(MFR)和残余尿量(P<0.05~0.00l),无明显副作用。结论通尿灵治疗前列腺增生症疗效肯定。 相似文献
102.
Endothelial cell transplantation and growth behavior of the human corneal endothelium 总被引:1,自引:0,他引:1
Katrin Engelmann Jürgen Bednarz Matthias Böhnke 《Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft》1999,96(9):555-562
Background: The human corneal endothelium has a limited proliferative capacity in vivo. Until now it has only been possible to replace
damaged endothelium by transplantation of a donor cornea. After establishing methods for the isolation and in vitro cultivation
of human corneal endothelial cells, transplantation of these cells my be an alternative therapeutic option.
Materials and methods: In this review methods for the in vitro cultivation of human corneal endothelial cells and their transplantation on the Descemet
membrane of donor corneas are described.
Results: In vitro proliferation of human adult corneal endothelial cells was achieved by the development of defined cell culture conditions,
including supplementation of culture medium with specified growth factors and substances. Dependent on the culture conditions,
as well as independent of them, in vitro cultured endothelial cells showed phenotypic changes and different proliferative
behavior. Thus, molecular biological examinations revealed a different expression pattern of growth factor receptors in fibroblast-like
endothelial cells (dedifferentiated) compared to typical endothelial cells (differentiated). Moreover, the proliferative capacity
of the cells differed, dependent on their corneal location. Cells isolated from the peripheral part of donor corneas have
a higher proliferative capacity than cells obtained from the central part. The propagation of corneal endothelial cells in
vitro offered the possibility of their transplantation on donor corneas in an in vitro model. After transplantation, these
cells formed a monolayer whose morphology and cell density depended on the differentiation of the cells. DNA synthesis was
predominantly detectable in cells of the corneal periphery.
Conclusions: Our findings are the basis of the following hypothesis: the periphery of the cornea represents a regenerative zone of the
corneal endothelium. The fact that early after transplantation corneal endothelial cells form a monolayer on the natural extracellular
matrix (ECM), which shows contact inhibition, suggests that inhibitory factors are released by the Descemet membrane that
influence the proliferation of the cells. Further studies on the regulation of the proliferation and differentiation of human
corneal endothelial cells in vitro and after transplantation might offer the possibility to establish a selective procedure
for the treatment of corneal endothelial cell loss in the near future.
相似文献
103.
V. De Sanctis A. Pinamonti A. Di Palma M. Sprocati G. Atti M. R. Gamberini C. Vullo 《European journal of pediatrics》1996,155(5):368-372
Nine transfusion-dependent -thalassaemia major patients (seven males and two females), aged 4–15 years, with growth retardation and severe rickets-like radiological lesions due to continuous subcutaneous chelation therapy with desferrioxamine (45–75 mg/kg body weight, 6–7 times/week), were seen in our centre during the last 8 years. Serum ferritin levels ranged from 976 to 4115 g/l. There was a progressive decline in growth velocity in these patients 2–3 years before the appearance of rickets-like radiological lesions. All patients underwent surgery to correct genu valgum and/or slipped capital epiphyses. The final height was below the 3rd percentile in six patients (SDS: from –2.9 to –5.2). The short stature was mainly due to a disproportion between upper and lower segments. Six of the patients had an associated sensorineural hearing loss.Conclusion Our data emphasize the importance of an accurate surveillance of the toxic effects of desferrioxamine treatment and warn of the risk of overtreating patients with low iron overload and also suggest a possible individual idiosyncrasy to the adverse effects of chelation therapy. 相似文献
104.
In this prospective study, birth weight of 304 babies born at Kamla Nehru Hospital Pune during study period was recorded.
From these 304 babies, babies with birth weight above 2000 grams were selected (260 babies) to prepare growth velocity curves.
Daily weight of these 260 babies was recorded for 30 days. The mean birth weight of study population was 2742.5 grams. Among
the daily weight recorded babies, all the babies lost weight ranging from 92 to 218 grams (mean 121 grams) after birth. The
weight loss continued upto 5 days. Days required to gain weight equal to birth weight ranged from 5 to 13 days. Total weight
gain observed in 30 days was 734.7 grams. Predictive value of these curves was tested in 49 infants. Deviation upto 50 grams
of predicted birth weight from actual birth weight was observed in 90% of babies on day-2, 79% on day-4, 65% on day-8 and
39% on day-30. 相似文献
105.
Systemic treatment with epidermal growth factor (EGF) induces growth of all wall layers in the urinary tract of pigs and rats. The present study was initiated to describe morphological and biochemical changes in the bladder smooth muscle from rats treated with EGF for 4 weeks. Eight-week-old female Wistar rats were treated with subcutaneous injections of vehicle (n=16) or EGF (n=8, 150 g/kg per day) for 4 weeks. After EGF treatment the bladders were increased in weight [74.4±0.4 vs 122.1±0.5 mg, P<0.001 (mean ± SEM)]. Sodium dedecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) analyses of six bladders from each group revealed that the total amounts of actin, myosin and desmin were statistically significantly increased by 62%, 61% and 154%, respectively. The relative amounts of actin and myosin were unchanged whereas the desmin to actin ratio was significantly increased — as previously described in rat bladder smooth muscle hypertrophy. Light and electron microscopy of two bladders from each group revealed increased wall thickness involving all wall layers. The smooth muscle fibres at a midventral bladder location seemed only slightly hypertrophic — some degree of hyperplasia was therefore suspected. In conclusion, EGF treatment for 4 weeks induced a net synthesis of contractile and cytoskeletal proteins in the urinary bladder smooth muscle. 相似文献
106.
Mark R. Benfield Angie Vail F. Bryson Waldo R. Pat Bucy Edward C. Kohaut 《Pediatric nephrology (Berlin, Germany)》1996,10(3):280-282
Recombinant human growth hormone (rhGH) improves growth in children after renal transplantation, but may be associated with augmented immune responses. We previously demonstrated that rhGH augments proliferative and cytotoxic responses and interferon-gamma (IFN-) mRNA expression during a mixed leukocyte culture (MLC). In this study, we evaluated 12 pediatric patients after receiving a renal allograft from one of their parents. Peripheral blood mononuclear cells (PBMC) were isolated from patients and cultured with either donor or unrelated third-party PBMC in an MLC. Patients developed significant donor-specific hyporesponsiveness (DSH), however, no correlation was seen between the amount of DSH and graft function. Of the 12 patients, 2 developed augmented responses in the presence of rhGH. rhGH augments proliferation, cytotoxicity, and IFN- expression during an MLC. Some patients develop increased responses to donorspecific alloantigens after renal transplantation. Further study is needed to better determine the significance of this finding. 相似文献
107.
108.
M. Caruso-Nicoletti M. Mancuso G. Spadaro S. P. Dibenedetto A. DiCataldo G. Schiliró 《European journal of pediatrics》1993,152(9):730-733
Growth impairment and growth hormone (GH) deficiency have been reported in children treated for acute lymphoblastic leukaemia (ALL). We have studied growth and GH secretion in a group of 50 patients, affected by ALL, during a 2- to 5-year period after diagnosis, and in 12 long-term-survivors. We observed a significant decrease in growth velocity during the 1st year (in particular during the first 6 months) of therapy and a catch-up growth after the end of therapy. Longterm survivors did not exhibit a significant reduction of height standard deviation score (SDS), as compared to height SDS at diagnosis. None of the patients showed GH deficiency. Our data indicate that chemotherapy significantly affects growth of patients treated for ALL, whereas radiotherapy-at the doses used in this study-does not induce GH deficiency, at least not within 9 years after diagnosis. 相似文献
109.
Septo-optic dysplasia and growth hormone deficiency: accelerated pubertal maturation during GH therapy 总被引:1,自引:0,他引:1
Susanna Freude H Frisch Daniela Wimberger Edith Schober Gabriele Häusler F Waldhauser F Aichner 《Acta paediatrica (Oslo, Norway : 1992)》1992,81(8):641-645
We report four patients (three male, one female) with septo-optic dysplasia and growth hormone deficiency. All had GH therapy for a period of four to eight years until reaching final height. In all four cases bone maturation during puberty was accelerated (1.4 to 1.9 "years"/year), resulting in a final height which was clearly below the predicted height. The progress of pubertal stages was very short in all patients. In three patients TSH and prolactin release after TRH stimulation were increased. These data support a hypothalamic original of the endocrine disorder. Insufficient GH release, even after repeated GHRH stimulation, is in contrast to this assumption. In one case there was a late manifestation of neurohormonal diabetes insipidus, which indicates the possibility of later disease progression. MR imaging of the brain demonstrated variable malformation of the septum pellucidum, chiasma and nervus opticus or the pituitary gland, respectively. 相似文献
110.
Carlo Orzincolo M.D. Pier Nuccio Scutellari M.D. Giuseppe Castaldi M.D. 《Skeletal radiology》1992,21(1):39-44
In 12 patients affected by thalassemia major who received an intensive transfusion regimen combined with continuous iron chelation therapy (desferrioxamine 50–80 mg/kg daily), radiologic abnormalities of the long bones were observed similar to those observed in rickets and scurvy. These abnormalities were associated with a growth retardation. The pathogenesis of these lesions is uncertain, but probably the toxic effect of desferrioxamine plays an important role in their development. A relative deficiency of vitamins D and/or C cannot be entirely excluded. 相似文献