全文获取类型
收费全文 | 38373篇 |
免费 | 2937篇 |
国内免费 | 1837篇 |
专业分类
耳鼻咽喉 | 461篇 |
儿科学 | 1085篇 |
妇产科学 | 623篇 |
基础医学 | 8549篇 |
口腔科学 | 503篇 |
临床医学 | 2941篇 |
内科学 | 6873篇 |
皮肤病学 | 707篇 |
神经病学 | 2248篇 |
特种医学 | 657篇 |
外国民族医学 | 6篇 |
外科学 | 3261篇 |
综合类 | 5423篇 |
现状与发展 | 6篇 |
预防医学 | 1772篇 |
眼科学 | 661篇 |
药学 | 2247篇 |
3篇 | |
中国医学 | 340篇 |
肿瘤学 | 4781篇 |
出版年
2024年 | 60篇 |
2023年 | 342篇 |
2022年 | 844篇 |
2021年 | 1017篇 |
2020年 | 962篇 |
2019年 | 863篇 |
2018年 | 861篇 |
2017年 | 993篇 |
2016年 | 1084篇 |
2015年 | 1207篇 |
2014年 | 1893篇 |
2013年 | 2180篇 |
2012年 | 2055篇 |
2011年 | 2374篇 |
2010年 | 2025篇 |
2009年 | 2134篇 |
2008年 | 2425篇 |
2007年 | 2514篇 |
2006年 | 2527篇 |
2005年 | 2426篇 |
2004年 | 2001篇 |
2003年 | 1873篇 |
2002年 | 1512篇 |
2001年 | 1364篇 |
2000年 | 1109篇 |
1999年 | 1005篇 |
1998年 | 809篇 |
1997年 | 693篇 |
1996年 | 433篇 |
1995年 | 418篇 |
1994年 | 284篇 |
1993年 | 198篇 |
1992年 | 119篇 |
1991年 | 114篇 |
1990年 | 78篇 |
1989年 | 67篇 |
1988年 | 62篇 |
1987年 | 32篇 |
1986年 | 31篇 |
1985年 | 33篇 |
1984年 | 19篇 |
1983年 | 11篇 |
1982年 | 24篇 |
1981年 | 19篇 |
1980年 | 18篇 |
1979年 | 8篇 |
1978年 | 3篇 |
1977年 | 7篇 |
1976年 | 3篇 |
1970年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 0 毫秒
101.
Stefan Somlo 《Clinical and experimental nephrology》1998,2(3):211-217
Conclusion The past decade has seen extraordinary progress in the study of autosomal-dominant polycystic kidney disease. The 2 major
genes for this disorder have been identified. Animal models of ADPKD have been produced. The molecular basis of the disease
has been characterized. ADPKD is a “second-hit” disease, much like many cancer predisposition syndromes. This has profound
implications for our understanding. The progression of ADPKD in individual patients is likely related more to their individual
rate of acquisition of second hits at thePKD1 orPKD2 locus than to the inherited germ line mutation itself. Therapeutic approaches will perhaps now be considered, which will
include interventions that may limit the rate at which somatic mutations occur in the kidney. The major focus of research
at present is to elucidate the normal functions ofPKD1 andPKD2. Protein binding partners are being sought for both proteins. The possible calcium channel function ofPKD2 is being investigated. The downstream effects of cellular deficiency of either protein are likely to yield many clues. Modifying
genetic factors that may independently affect disease progression are likely to be identified using the several mouse models.
Perhaps the next decade will bring great strides in understanding and in potential therapy for this common disease.
This paper was presented at the 2nd International Forum “The Frontiers of Nephrology,” Tokyo, May 10, 1998. 相似文献
102.
胃癌c-erbB-2过度表达与预后的关系 总被引:1,自引:0,他引:1
探讨c-erbB-2过度表达与胃癌预后的关系。方法:用免疫组化ABC法对103例胃癌手术标本及151个转移淋巴结进行c-erbB-2表达检测。结果:21.4%胃癌手术标本出现阳性表达.其中进展期胃癌、乳头状腺癌、高中分化胃癌及伴淋巴与肝转移的胃癌阳性率显著增高(P<0.05与<0.01);转移淋巴结表达阳性率高于胃癌原发灶(X2=3.7.P>0.05)。高中分化胃癌伴c-erbB-2过度表达者5年生存率显著低于阴性者(P<0.01)。结论:c-erbB-2过度表达可作为胃癌预后估计指标之一。 相似文献
103.
Globoid cell leukodystrophy (GLD, Krabbe disease) is a severe demyelinating disease caused by a genetic defect of beta-galactocerebrosidase (GALC). To date treatment to GLD is limited to hematopoietic stem cell transplantation. Experimental approaches by means of gene therapy in twitcher mouse, an authentic murine model of human GLD, showed significant but only marginal improvements of the disease. To clarify whether the introduction of GALC could provide beneficial effects on the oligodendrocytes in GLD, we transduced twitcher oligodendrocytes by stereotactically injecting recombinant retrovirus encoding GALC-myc-tag fusion gene into the forebrain subventricular zone of neonatal twitcher mouse. In vivo effects of exogenous GALC on twitcher oligodendrocytes were studied histologically by combined immunostaining for the myc-epitope and the oligodendroglial specific marker, pi form of glutathione-S-transferase, at around 40 days of age. We show here that GALC transduction led to dramatic morphological improvement of the twitcher oligodendrocytes comparing with those in untreated twitcher controls. This study provided direct in vivo evidence that GALC transduction could prevent or correct aberrant morphology of oligodendrocytes in GLD which may be closely related to the dysfunction and/or degeneration of oligodendrocytes and the demyelination in this disease. 相似文献
104.
Loeys B De Backer J Van Acker P Wettinck K Pals G Nuytinck L Coucke P De Paepe A 《Human mutation》2004,24(2):140-146
In order to estimate the contribution of mutations at the fibrillin-1 locus (FBN1) to classical Marfan syndrome (MFS) and to study possible phenotypic differences between patients with an FBN1 mutation vs. without, a comprehensive molecular study of the FBN1 gene in a cohort of 93 MFS patients fulfilling the clinical diagnosis of MFS according to the Ghent nosology was performed. The initial mutation screening by CSGE/SSCP allowed identification of an FBN1-mutation in 73 patients. Next, sequencing of all FBN1-exons was performed in 11 mutation-negative patients, while in nine others, DHPLC was used. This allowed identification of seven and five additional mutations, respectively. Southern blot analysis revealed an abnormal hybridization pattern in one more patient. A total of 23 out of the 85 mutations identified here are reported for the first time. Phenotypic comparison of MFS patients with cysteine-involving mutations vs. premature termination mutations revealed significant differences in ocular and skeletal involvement. The phenotype of the eight patients without proven FBN1 mutation did not differ from the others with respect to the presence of major cardiac, ocular, and skeletal manifestations or positive familial history. Most likely, a portion of FBN1-mutations remains undetected because of technical limitations. In conclusion, the involvement of the FBN1-gene could be demonstrated in at least 91% of all MFS patients (85/93), which strongly suggests that this gene is the predominant, if not the sole, locus for MFS. 相似文献
105.
Single nucleotide polymorphisms in the protamine-1 and -2 genes of fertile and infertile human male populations 总被引:6,自引:0,他引:6
Tanaka H Miyagawa Y Tsujimura A Matsumiya K Okuyama A Nishimune Y 《Molecular human reproduction》2003,9(2):69-73
Although various genetic factors have been implicated in human male infertility, the causative genes for the different types of idiopathic male infertility have not been elucidated. Protamines, which are the major DNA-binding proteins in the sperm nucleus, package the DNA into the sperm head. Analysis of the human protamine-1 (PRM1) and -2 (PRM2) gene sequences in 226 sterile male patients and in 270 proven-fertile male volunteers revealed four single nucleotide polymorphisms (SNPs) in the PRM1 coding region, which did not cause any amino acid substitutions, and one SNP in the PRM2 gene, which produced translation termination. We also observed one SNP in the 3' non-coding region of the PRM1 gene, and two SNPs within the intron of the PRM2 gene. The prevalence of these SNPs was similar in both infertile patients and in proven-fertile volunteers, except that the c248t alteration in the PRM2 gene induced a nonsense codon under conditions of heterozygosity in one infertile patient. Although the PRM1 and PRM2 genes are highly conserved, the single SNP in the PRM2 gene that induces translation termination may result in male infertility due to haploinsufficiency of PRM2. 相似文献
106.
Fiona Campbell MD MRCPath John M Geraghty MBBS MRCPath Mark A.C Appleton MBChB MRCPath E.Dillwyn Williams MD FRCP FRCPath Geraint T Williams MD FRCP FRCPath 《Human pathology》1998,29(12):1531-1535
Colorectal tumorigenesis in familial adenomatous polyposis (FAP) results from somatic mutation of either the normal APC allele or another growth control gene in epithelial cells bearing a germline APC defect. The rate at which tumors develop is therefore dependent on the somatic mutation frequency; it is not known whether this is normal or elevated in FAP. We aimed to quantify stem cell somatic mutation in FAP, comparing it with hereditary nonpolyposis colorectal cancer (HNPCC) and Crohn's disease (CD). Stem cell somatic mutation frequency was studied in 47 FAP patients, 5 HNPCC patients, and 13 CD patients, all younger than 49 years, by quantifying crypt-restricted loss of O-acetyltransferase activity in sections of morphologically normal colonic mucosa from individuals heterozygous for this monogenically inherited polymorphism. Median stem cell somatic mutation frequency was significantly higher in FAP than HNPCC (4.2 × 10−4v 1.4 × 10−4, Mann-Whitney U, P < .02). The level in CD (4.0 × 10−4) was similar to FAR Mutated crypts occurred in groups more frequently in FAP (22%) than HNPCC (12%) or CD (10%), suggesting an increase in stem cell division associated with crypt fission in FAP. We conclude that stem cell somatic mutation frequency is raised in non-neoplastic colorectal mucosa in FAR This is probably related to increased stem cell proliferation and contributes to the high rate of tumor formation in this condition. 相似文献
107.
经丝裂霉素C体外处理后,将IL-6基因转染的、高分泌的IL-6的B16黑色素瘤细胞制成瘤苗。结果发现,体内注射IL-6基因转染的瘤苗后,小鼠脾脏CTL活性、NK活性及IL-2诱导的LAK活性显著升高。经IL-6基因转染瘤苗体内治疗后,荷瘤小鼠的皮下肿瘤生长显著减慢、肺转移结节数显著降低、存活期显著延长,若同时合用低剂量IL-2,则上述治疗效果更好。可见IL-6基因转染的瘤苗能有效地通过诱导机体抗肿瘤免疫功能而发挥抗肿瘤作用,与低剂量IL-2合用后,IL-6基因转染的瘤苗的抗肿瘤效果更佳。 相似文献
108.
Summary From a gene bank ofS. pombe DNA, a 5.6 kb clone was isolated which complemented mutants defective in glutamine synthetase (GS) activity. Sub-cloning
fragments of this 5.6 kb clone showed that the complementing activity was localised in a 1.6 kb HindIII-Aval fragment and
a partial DNA sequence revealed an open reading frame preceded by TATA sequences and a TGACTA sequence. Plasmid constructs
carrying up to 3.4 kb of DNA used to transformgln
− strains gave transformants which showed a wide range of GS activity, in some cases 100 times the wild-type level. These constructs
identify DNA sequences lying downstream from the putative coding sequence which have effects on the total amount of enzyme
activity, but do not affect the control imposed by the nitrogen source on which the cells are grown. 相似文献
109.
Class switch recombination (CSR), somatic hypermutation, and gene conversion are immunoglobulin diversification mechanisms that are strictly dependent on the activity of the activation-induced cytidine deaminase (AID). The precise role and substrate(s) of AID in these processes remain to be well defined. The closest homologue of AID is APOBEC-1, a bona fide mRNA-editing enzyme, which shares with AID the ability to deaminate cytidines within single-stranded DNA in vitro and in prokaryotic cells. To determine whether APOBEC-1 can therefore substitute for AID in activated B cells, we expressed human AID, a catalytic mutant thereof, and rat APOBEC-1 in AID-deficient murine B cells. Whereas AID rescued CSR, neither the inactive mutant nor APOBEC-1 could complement AID deficiency. This indicates that cytidine deaminase activity is necessary but not sufficient to initiate CSR, and suggests that AID is specifically targeted to its cognate substrate, the immunoglobulin genes or a distinct mRNA, by an as-yet-unknown mechanism. 相似文献
110.
本文采用基因探针检测孕妇静脉血及新生儿脐静脉血白细胞中CMVDNA,同时应用ELISA检测其血清中IgG、IgM.结果:10例孕妇CMV-DNA阳性,占8.7%(10/115),这10例孕妇血IgG均阳性,IgM均阴性;17例新生儿CMV—DNA阳性.占14.3%(17/119),其中IgM阳性2例,占1.7%(2/119)。本次研究中配对者79例,母儿CMV—DNA均阳性者8例,新生儿CMV—DNA阳性而母亲阴性者4例。上述CMV-DNA阳性者均无临床症状。研究结果提示基因探针可监测CMV的潜伏感染,从而进一步研究CMV感染的母儿传播机制。 相似文献