超声波提取快速测定复混肥料中的水溶性磷

研究超声波提取复混肥料中水溶性磷的方法及提取条件对测定结果的影响,该方法与国家标准GB150632001《复混肥料》中规定的方法比较,操作简单、省时,准确度较高,RSD值小于0.5%。     

A rapid solid-phase extraction and HPLC/DAD procedure for the simultaneous determination and quantification of different benzodiazepines in serum,blood and post-mortem blood

Summary A rapid and quantitative method for the determination of benzodiazepines using high-performance liquid chromatography (HPLC) with diode-array detection (DAD) is reported. The drugs were extracted from serum, blood or post-mortem blood using C₁₈ extraction columns. Brotizolam was used as internal standard. Experiments with spiked serum/blood samples resulted in recoveries between 75% and 94% for all investigated benzodiazepines. Excellent linearity was obtained over the concentration range 5–1500 ng benzodiazepine/ml. The limit of detection was approximately 2 ng/ml. The detection of low therapeutic serum levels of highly potent benzodiazepines is also possible.      

CRITERIA ADOPTED BY DENTISTS TO INDICATE THE EXTRACTION OF PERIODONTALLY INVOLVED TEETH

When dealing with patients with periodontal disease of variable severities, dentists must often choose between treating and restoring the involved tooth or indicating its extraction. Different criteria have been adopted in this decision-making process. The purpose of this study was to evaluate the criteria adopted by dentists to indicate the extraction of teeth with periodontitis. Dentists were interviewed at their private practices in three cities of the state of Rio Grande do Sul, Brazil. The evaluated criteria included severity of attachment loss, tooth mobility, furcation involvement, prosthetic planning, periodontal-endodontic lesion, possible systemic involvement due to the presence of periodontitis, referral to a periodontist for evaluation, radiographic bone loss greater than 50%, presence of extensive caries, socio-economic and cultural status of the patient, among others. The most often adopted criteria to indicate the extraction of periodontally affected teeth were the presence of mobility (37.5%), severity of attachment loss (24.3%) and radiographic bone loss greater than 50% (21.2%). The results of the present study demonstrated the difficulties faced by dentists to indicate the extraction of teeth with severe attachment loss, in addition to the establishment of an adequate prognosis. Aspects associated with the past disease were still the most often reported to indicate the extraction of teeth for periodontal reasons.     

削梨法在小瞳孔白内障超声乳化术中的应用

目的：探讨小瞳孔状态下应用削梨法超声乳化的手术技巧和注意事项。方法：应用削梨法超声乳化技术对69例(78只眼)小瞳孔白内障行超声乳化。结果：无虹膜后粘连63只眼，术后全部瞳孔恢复原状；15只眼分离粘连机化膜后应用削梨法进行超声乳化，术后13只眼基本恢复圆瞳孔，2只眼瞳孔不规则。术后3个月裸眼视力≥05者43只眼(55．13％)．矫正视力≥05者61只眼(78．21％)。结论：削梨法受瞳孔大小的限制较小，大大降低了手术操作的难度，减少了手术的危险性，不失为小瞳孔白内障超声乳化的好办法。     

微波辅助提取茯苓中茯苓多糖的研究

目的将微波辅助提取新技术应用于茯苓水溶性多糖的提取，寻求最佳提取工艺。方法采用均匀优化设计试验条件，以苯酚-硫酸法测定样品中多糖含量。对超声辅助提取和传统水提法比较，并对水解前后的微波提取多糖衍生物用毛细管电泳检测。结果最佳提取条件：时间为18min；固液比为1：50；微波占空比42％，此时提取率为2．792％。优于传统。结论微波辅助提取速度更快、提取效率更高；毛细管电泳检测表明微波提取茯苓多糖过程合理，能得到较理想的提取物。     

转基因食品的快速PCR鉴定

用十六烷基-二甲基-乙基-溴化铵(CTAB)法快速高效地从样品中提取了可供分析的基因组DNA,经PCR扩增,鉴定了含有35S启动子和NOS终止子的转基因样品.该方法的灵敏度可达0.1%,并且具有很好的稳定性.     

粘连性小瞳孔白内障超声乳化吸除及人工晶状体植入术

目的探讨粘连性小瞳孔白内障超声乳化吸除手术方法及技巧。方法对36例(42眼)粘连性小瞳孔白内障行超声乳化吸除及人工晶状体植入术,术中不分离虹膜后粘连,利用拦截劈裂法乳化晶状体核,通过4mm大小瞳孔完成手术。结果所有病例视力有不同程度提高,获得生理性圆或近圆瞳孔,主要并发症包括角膜内皮水肿,前房纤维素渗出。结论采用本方法治疗粘连性小瞳孔白内障操作相对简单,术后反应轻,并发症少。     

Astigmatism and visual recovery after phacoemulsification and conventional extracapsular cataract extraction

In this study we wanted to investigate the post-operative astigmatism and visual acuity after phacoemulsification and conventional extracapsular cataract surgery. Patients operated between April and June 1993 (n=150) were retrospectively analyzed. The patients were examined prior to surgery and at day 1, at day 10, and in week 6 post-operatively. The difference between the post-operative log mean visual acuity in the Phaco group and in the CECCE group was significant after 1 and 10 days, however it was not significant (p=0.191) after 6 weeks. The mean astigmatism was significantly less in the Phaco group than in the CECCE group during the whole post-operative check-up period. This study suggests that Phaco results in a lower post-operative astigmatism and an earlier visual rehabilitation compared to the CECCE technique.Abbreviations Phaco Phacoemulsification - CECCE Conventional extracapsular cataract extraction     

Comparison between postoperative astigmatism after classic extracapsular lens extraction and after phacoemulsification with implantation of a Pearce tripod or Pearce vaulted Y-loop intraocular lens

We compared the surgically induced astigmatism after standard extracapsular cataract extraction (ECCE) with the astigmatism following cataract extraction by phacoemulsification. The surgically induced corneal astigmatism was assessed on several occasions, ranging from one day to one year postoperatively. After phacoemulsification, this astigmatism was considerably slighter than after ECCE on day 1 postoperatively and after two and six weeks. However, one year postoperatively, this difference was less clear. We then measured slight against-the-rule astigmatism for both surgical techniques.     

离子对提取比色法测定复方制剂中氨基比林的含量

复方制剂中氨基比林与溴甲酚绿形成稳定的离于对，用氯仿萃取后在４１６．８ｎｍ处测定吸收度．回收率为９９．８０％，相对标准偏差为０．９２％（ｎ＝５）。方法简便快速，结果准确可靠。