固定化酵母细胞生产1，6－二磷酸果糖的动力学研究

筛选获得一株１,６－二磷酸果糖（ＦＤＰ）的高产酵母菌株,改变通透性后的酵母细胞,在发酵及固定化生产ＦＤＰ过程中发现Ｆｅ２＋和甘油能大量提高ＦＤＰ的积累。将戊二醛交联后的酵母细胞,用卡拉胶固定化后,装入酶反应柱,连续生产ＦＰＤ,柱反应可稳定在１０天以上,转化率维持在２０％以上。     

1,6二磷酸果糖佐治小儿肺炎合并心力衰竭临床观察

目的：观察1，6二磷酸果糖（FDP）佐治小儿肺炎合并心力衰竭的临床疗效。方法：选择太原市太航医院儿科肺炎合并心力衰竭诊断明确的住院患儿76例，按年龄、性别、临床表现随机分为治疗组和对照组各38例，对照组给予常规的综合治疗和西地兰纠正心力衰竭，治疗组在对照组基础上加用FDP静滴，进行疗效比较。结果：治疗组疗效明显优于对照组，经统计学处理两组差异有统计学意义（P〈0．01）。结论：FDP佐治小儿肺炎合并心力衰竭疗效显著，值得推广。     

An investigation of two multivariate permutation methods for controlling the false discovery proportion

Identifying genes that are differentially expressed between classes of samples is an important objective of many microarray experiments. Because of the thousands of genes typically considered, there is a tension between identifying as many of the truly differentially expressed genes as possible, but not too many genes that are not really differentially expressed (false discoveries). Controlling the proportion of identified genes that are false discoveries, the false discovery proportion (FDP), is a goal of interest. In this paper, two multivariate permutation methods are investigated for controlling the FDP. One is based on a multivariate permutation testing (MPT) method that probabilistically controls the number of false discoveries, and the other is based on the Significance Analysis of Microarrays (SAM) procedure that provides an estimate of the FDP. Both methods account for the correlations among the genes. We find the ability of the methods to control the proportion of false discoveries varies substantially depending on the implementation characteristics. For example, for both methods one can proceed from the most significant gene to the least significant gene until the estimated FDP is just above the targeted level ('top-down' approach), or from the least significant gene to the most significant gene until the estimated FDP is just below the targeted level ('bottom-up' approach). We find that the top-down MPT-based method probabilistically controls the FDP, whereas our implementation of the top-down SAM-based method does not. Bottom-up MPT-based or SAM-based methods can result in poor control of the FDP.     

Thrombin generation in patients with thrombotic thrombocytopenic purpura

Thrombotic thrombocytopenic purpura (TTP) is thought to be caused primarily by endothelial cell injury or primary platelet agglutination. A coagulation screen usually shows normal or minimal changes, but a modest elevation of fibrinogen/fibrin degradation products (FDP) is observed in many patients with TTP. To assess the thrombin generation in vivo in TTP, plasma levels of thrombin-antithrombin III complex (TAT) were measured together with plasmin-alpha 2-antiplasmin complex (PAP) in ten patients with acute TTP. Plasma TAT [mean 6.7 +/- (SD) 3.7 micrograms/liter] as well as PAP (2.1 +/- 1.2 mg/liter) were elevated in patients with TTP as compared with healthy subjects (TAT of 1.7 +/- 0.3 microgram/liter and PAP of 0.2 +/- 0.1 mg/liter; n = 10). These findings indicate that considerable amounts of thrombin and plasmin are actually generated in TTP, although the majority of patients do not show signs of consumption coagulopathy.     

1，6－二磷酸果糖对培养心肌细胞膜缺氧－再灌注损伤的保护作用

用培养的原代心肌细胞建立缺氧－再给氧方法，对缺氧６０分钟、１２０分钟及缺氧后再给氧３０及６０分钟时心肌细胞搏动、ＬＤＨ活性、（５１）￣Ｃｒ释放率、苔盼蓝摄取率、扫描电镜观察及１，６－二磷酸果糖对其影响进行了研究。结果显示：缺氧２小时可引起心肌细胞搏动频率减慢，再给氧后搏动频率加快；ＬＤＨ活性、（５１）￣Ｃｒ释放率和苔盼蓝摄取率增加，电镜显示心肌细胞膜受损。１，６－二磷酸果糖对心肌细胞膜具有保护作用。     

Tendon ruptures: mallet, flexor digitorum profundus

Mallet injuries are the most common closed tendon injury in the athlete. Flexor digitorum profundus ruptures are rare in baseball, but are common injuries in contact sports. The diagnosis for each condition is based on clinical examination, although radiographs should be evaluated for a possible bony component. Treatment for mallet injury depends on the athlete's goals of competition and understanding of the consequences of any treatment chosen. Gripping, throwing, and catching would be restricted or impossible with the injured finger immobilized. Treatment of FDP ruptures is almost always surgical and requires reattachment of the torn tendon to the distal phalanx.     

血液纤维蛋白(原)降解产物检测方法的比较研究

1　临床资料与方法1.1　一般资料随机选择在本院各科住院病人 2 0 0例 ,男 117例 ,女 83例 ;平均年龄 4 2岁 (2 0～ 79岁 )。随机选择正常人 2 6例 ,男 12例 ,女 14例 ;平均年龄 2 6岁(18～ 4 8岁 )。正常人 2周内未服用过任何药物 ;所有女性正常人均避开了月经期 ;既往无静脉血栓、心梗及肝功能障碍等疾病。1.2　方法采用乳胶凝集半定量法。以抗ＦＤＰ单克隆抗体及抗ＦＤＰ多克隆抗体标记的乳胶颗粒与待测样品混合 ,当标本中ＦＤＰ含量大于正常时 ,标记的乳胶颗粒凝集 ,判断为阳性反应。1.3　样品制备抽取静脉血分三管 ,即血清、血浆和ＥＡ…     

急性早幼粒细胞白血病患者FG、SFC、FDP及D-二聚体的检测

测定40例正常人和50例急性早幼粒细胞白血病患者血浆纤维蛋白原（Fg）、血清纤维蛋白降解产物（FDP），血浆可溶性纤维蛋白复合物（SF）及血浆D-二聚体的含量．结果：Fg为1．8±0．72g／L，FDP为464±94μg／L，SFC为482．5±167mg／L，D-二聚体为5524.94±4247．03μg／L．正常对照组Fg为3．2±0．87g／L．FDP为215±63μg／L，SFC为49．7±16．4mg／L，D-二聚体为177．1±43．9ug／L．两组差异显著．提示上述指标可作为显示体内出凝血像异常及纤溶亢进的有效证据．作为DIC的确诊可确切地反应疾病病变过程．     

Red cell pyruvate kinase deficiency: An optimised assay

As a result of using a variety of assay techniques, rather different values for the rate of the pyruvate kinase (PK) reaction were obtained. Kinetic measurements performed with red cell PK using the stopped-flow as well as the “classical” spectrophotometric method demonstrated that only the initiation of the reaction with ADP yields reproducible results in the standard assay. Moreover, the influence of substrate concentrations, activator concentrations, ionic strength, buffer system, temperature and duration of preincubation were thoroughly investigated and were shown to play an important role. As a consequence of our kinetic measurements, a modified optimised assay composition together with new normal values for erythrocyte PK activity in haemolysate are presented. For better characterisation and comparability of PK variants, we suggest the use of the optimised assay composition described and that the reaction be initiated with ADP.     

血浆D-二聚体和FDP检测在儿童过敏性紫癜中的临床意义

血浆中D-二聚体和纤维蛋白原降解物（FDP）浓度是准确判定机体血凝和纤溶状态的理想分子标记物,在儿童过敏性紫癜（HSP）的临床诊断和病情观察中具有重要意义.本研究测定了136例HSP患儿血浆D-二聚体和FDP浓度,结果发现血浆D-二聚体和FDP的阳性率分别为52.94%和51.47%,均显著高于对照组（P〈0.05）;同时血浆中D-二聚体和FDP的绝对浓度分别为1.46±0.41μg/mL和11.75±3.12μg/mL,也显著高于对照组（P〈0.05）.然而,在HSP不同临床表型组间,阳性率和绝对浓度两个指标均不存在显著差异（P〉0.05）.本研究结果支持HSP患儿具有显著升高的血浆D-二聚体和FDP阳性率和绝对浓度,可以用于疑似HSP患儿的临床诊断,也可监测血液凝溶功能的变化情况.