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31.
目的:探讨腺苷对心肌梗死再灌注无复流的保护作用,以及这种保护作用与腺苷对一氧化氮(NO)和内皮素(ET)影响的关系。方法:制作离体兔心急性心肌梗死模型。30只兔心随机分为三组:A:假手术组,B:心肌梗死再灌注组,C:腺苷+心肌梗死再灌注组。分别取灌流开始5min和再灌流90min时冠脉流出液2ml,测定乳酸脱氢酶(LDH)、肌酸激酶(CK)、内皮素(ET)及一氧化氮(NO)含量。灌流结束时,测量无复流区域面积的百分比,并在光镜下观察心肌细胞的变化情况。结果:(1)A组灌流开始与结束时和B组、C组开始灌流时冠脉流出液中LDH、CK含量相比无显著性差异;(2)B组各项化验指标分别进行组内比较其结果有统计学差异。(3)灌流结束时,B组与C组相比NO的降低和ET的升高有统计学差异。(4)C组和B组比较无复流区域面积百分比明显缩小,有统计学差异。结论:腺苷对心肌梗死再灌注无复流具有明显的保护作用。腺苷升高NO和降低ET的作用可能是其发挥保护作用的原因之一。 相似文献
32.
冠心康对实验性家兔动脉粥样硬化血清NO及血浆ET-1的影响 总被引:6,自引:0,他引:6
目的 :观察中药冠心康预防用药对食饵性动脉粥样硬化 (AS)家兔血清一氧化氮 (NO)及血浆内皮素 1(ET 1)的影响。方法 :采用猪油加胆固醇饲料造模法 ,建立家兔动脉粥样硬化模型。随机分为正常对照组 (N组 ) ,模型组 (M组 ) ,冠心康治疗组 (T组 ) ,复方丹参片治疗对照组 (C组 ) ,各组在实验开始 ,第 6周末 ,第 12周末取血标本测定上述参数。结果 :与M组比较 ,T组、C组均可显著提高血清NO含量 ,降低血浆ET 1含量 (P <0 .0 5 ) ,且T组与C组比较有显著差异(P <0 .0 5 )。结论 :冠心康有维持内源性ET 1和NO的平衡而保护正常内皮功能的作用 ,这可能是其防治冠状动脉粥样硬化和抗心肌缺血作用的机制之一 相似文献
33.
Catherine Van Renterghem Michel Lazdunski 《Pflügers Archiv : European journal of physiology》1992,420(5-6):417-423
A small conductance K+ channel was identified in smooth muscle cells of the rat aortic cell line A7r5 and also in rat aortic smooth muscle cells in primary culture, using conventional single-channel recording techniques. The single-channel conductance shows no rectification, either in the range –70 to +40 mV under asymmetrical conditions (9.1 pS), or in the range –100 to +50 mV in symmetrical 150 mM K+ (37 pS). Channel activity is reversibly inhibited by extracellular application of charybdotoxin, with a concentration of 8 nM producing half-maximal inhibition. It is unaffected by apamin or scyllatoxin. Channel activity depends on the presence of free Ca2+ on the cytosolic face of the membrane, with an activation zone between 0.1 and 1 M. This small-conductance, charybdotoxin-sensitive, Ca2+-regulated K+ channel is activated by vasoconstrictors such as vasopressin and endothelin. 相似文献
34.
充血性心力衰竭治疗前后内皮素,肿瘤坏死因子和血管紧张素转化酶含量比较 总被引:12,自引:9,他引:3
目的探讨充血性心力衰竭(CHF)患儿不同心功能级数及分组治疗前后血浆ET、TNF和ACE含量的变化及其意义。方法用放免法和比色法分组测定充血性心力衰竭(CHF)80例患儿血中ET、TNF和ACE含量的变化。结果80例CHF患儿中血中ET、TNF和ACE含量分别为(141.33±8.56)ng/L、(168.25±35.14)ng/L和(429.45±61.33)IU,与正常对照组比较均明显升高(P<0.05);心功能Ⅱ级与心功能Ⅲ级、心功能Ⅳ级TNF、ET含量间比较均有显著性差异(P<0.05~0.01),相关有显著性意义;不同心功能级数CHF患儿血浆TNF、ET和ACE含量治疗后较治疗前明显降低,均有显著性差异(P<0.05~0.01),而且心功能越差,血浆TNF、ET水平恢复越慢,洛汀新组心功能改善较常规组明显,两者含量间比较有显著性差异(P<0.05)。结论血浆ET、TNP和ACE含量的测定有助于判断心力衰竭的程度,洛汀新组的疗效明显优于常规组。 相似文献
35.
乌拉地尔对重症高血压患者血浆降钙素基因相关肽、内皮素、神经肽Y含量的影响 总被引:4,自引:2,他引:2
目的:观察乌拉地尔(Ura)对重症高血压(CH)患者血浆降钙素基因相关肽(CGRP)、内皮素(ET)、神经肽Y(NPY)含量的影响。方法:用放射免疫分析方法测定30例CH患者血浆CGRP、ET、NPY水平,并以40例健康者作对照。观察CH患者静脉注射Ura(0.4-0.6mg/kg)后诸指标变化。结果:CH患者血浆CGRP水平显著低于健康对照组(P<0.01),ET和NPY明显高于健康对照组(均P<0.01)。经Ura治疗后CH患者CGRP水平明显升高,ET、NPY水平明显下降(均P<0.01)。结论:CGRP,ET和NPY是参与CH发病的重要体液因素,Ura可明显提高CH患者体内CGRP水平,有效拮抗ET和NPY的缩血管作用,对改善CH患者血流动力学和心肌氧供需失平衡有重要作用。 相似文献
36.
游泳训练大鼠血浆生物活性肽含量的变化及意义 总被引:5,自引:0,他引:5
目的研究运动性心肌肥大形成中血浆生物活性肽的含量及意义.方法wistar大鼠分为运动训练组和对照组,NPY、CGRP、ET和ANP用放射免疫方法测定,血压和心率用八道生理记录仪记录.结果(1)运动组大鼠游泳训练8周后,心脏系数比对照组增加36.8%(P<0.05),而收缩压、舒张压、平均动脉压和心率与对照组无差异(P>0.05);(2)运动组心肌肥大大鼠血浆NPY均比照组降低88%(P<0.01);(3)运动组心肌肥大大鼠血浆CGRP比对照组升高31%(P<0.01);(4)运动组血浆ET和ANP浓度与对照组无差异.结论运动性心肌肥大形成中血浆的CGRP、NPY含量变化不同,提示CGRP和NPY对运动性心肌肥大的形成可能有重要作用. 相似文献
37.
化湿汤对冠心病患者血浆心钠素、内皮素及 血脂的影响及临床观察 总被引:2,自引:0,他引:2
应用化湿汤治疗50例痰浊型冠心病(CHD)患,并与冠心Ⅱ号方治疗血瘀型冠心病患50例作对照。结果表明:两组皆取得较好的疗效。用药后不仅心绞痛等症状明显缓解,而且能改善心电图,降低血浆心钠素(ANP),内皮素(ET)和血脂(TC、TG)等临床客观指标。其作用机制可能与阻滞细胞Ca^ 内流有关。 相似文献
38.
Hans-Reinhard Zerkowski Andrea Broede Klaus Kunde Steffen Hillemann Ellen Schäfer Magdalene Vogelsang Martin C. Michel Otto-Erich Brodde 《Naunyn-Schmiedeberg's archives of pharmacology》1993,347(4):347-352
Summary The receptor systems through which serotonin (5-HT), histamine, angiotensin II and endothelin increase the force of contraction were studied in isolated right atria from patients without apparent heart failure.All agonists increased the atrial force of contraction in a concentration-dependent manner; maximal effects, however, were significantly less than those evoked by isoprenaline or Ca2+. 5-HT and histamine, but not angiotensin II and endothelin, activated adenylate cyclase, whereas endothelin and angiotensin II stimulated inositol phosphate generation. Experiments with subtype-selective antagonists revealed that histamine effects were mediated by H2-receptors (sensitive to ranitidine), 5-HT-effects by 5-HT4-receptors (sensitive to SDZ 205-557) and angiotensin II effects by AT1-receptors (sensitive to losartan).We conclude that in human right atria the force of contraction can be increased by cyclic AMP-dependent (histamine, 5-HT) and -independent (angiotensin II, endothelin) pathways. Compared to -adrenoceptors, however, all other receptor systems increase the force of contraction only submaximally indicating that the -adrenoceptor pathway is the most important physiological mechanism to regulate force of contraction and/or heart rate in the human heart.Correspondence to O. E. Brodde at the above address 相似文献
39.
Xie-Nan Huang Tetsuhiro Hisayama Issei Takayanagi 《Naunyn-Schmiedeberg's archives of pharmacology》1990,341(1-2):80-87
Summary The modes by which Endothelin-1 (ET) induces Ca2+-influx and the relative functional importance of the different sources of Ca2+ for ET-induced contraction were studied using fura 2-loaded and unloaded rat aortic strips. ET caused an increase in the cytosolic free Ca2+ level ([Ca2+]i) followed by a tonic contraction in Ca2+-containing solution, and produced a transient elevation of [Ca2+]i followed by a small sustained contraction in Ca2+-free medium. ET also stimulated 45Ca influx into La2+-inaccessible fraction significantly. With the same change of [Ca2+]i, ET caused a larger tension than that induced by high K. ET-induced contraction and [Ca2+]i elevation were not significantly inhibited by 0.1–0.3 M nicardipine which nearly abolished the contraction and [Ca+]i elevation produced by high K. During treatment of the strips with high K, addition of ET induced further increases in [Ca2+]i and muscle tension, and vice versa. In Ca2+-free medium, ET-induced contraction was influenced neither by ryanodine-treatment nor by high K-treatment, although the former attenuated and the latter potentiated the [Ca2+]i transient induced by ET. Further, the ET-induced sustained contraction under Ca2+-free conditions began to develop after the [Ca2+]i level returned to the baseline. Thus, it seems that the Ca2+ released from the ryanodine-sensitive and -insensitive Ca2+ stores by ET may provide only a minor or indirect contribution, if any, to the tension development. ET might cause a contraction mainly by stimulating Ca2+-influx through Ca2+ channel(s) other than voltage-dependent Ca2+ channels in character, and by increasing the sensitivity of the contractile filaments to Ca2+ or activating them Ca2+-independently.Visiting from Zun Yi Medical College, China
Send offprint requests to I. Takayanagi at the above address 相似文献
40.
川芎嗪对肾小球疾病患者血浆内皮素和降钙素基因相关肽的影响 总被引:2,自引:0,他引:2
目的:通过研究川芎嗪(tetramethyl pyrazine,TMP)对肾泪示疾病血浆内皮素(endothelin-1,ET-1)和降钙素基因相关肽(calcitonin gene related peptide,CGRP)的影响,探讨川芎嗪改善肾脏血液循环,保护肾功能的机理。方法:对肾小球疾病病例在同等治疗基础上,治疗组加用川芎嗪,对照组不用川芎嗪,分别检测两组病人治疗前和治疗后血浆中ET-1和CGRP含量。结果:治疗组治疗后与治疗前比较血浆中的ET-1下降和CGRP上升,均有特别显著性差异;对照组治疗前后比较,血浆中ET-1下降和CGRP上升,显著性差异。结论:川芎嗪治疗肾小球疾病可能是通过减少内皮细胞ET-1的产生和促进CGRP的生成,改善肾脏血液循环,对肾功能起保护作用。 相似文献