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31.
目的建立测定复方愈酚麻黄糖浆中盐酸麻黄碱、愈创甘油醚、马来酸氯苯那敏、双氯芬酸含量的HPLC方法。方法采用Insteril ODS-3C18色谱柱,梯度洗脱,流速:1.0mL·min^-1,柱温:30℃,检测波长:257nm。结果盐酸麻黄碱、愈创甘油醚、马来酸氯苯那敏、双氯芬酸分别在0.4018~6.4287μg(r=0.9999)、3.1836~50.9379μg(r=0.9999)、0.1610~2.5762μg(r=1.0000)、0.1485~2.3759μg(r=0.9999)范围内线性关系良好,平均回收率分别为99.589/6(RSD=0.39%)、99.58%(RSD=0.30%)、99.36%(RSD=0.29%)、99.40%(RSD=0.46%)(n=9)。结论本方法经方法学验证可用于复方愈酚麻黄糖浆中盐酸麻黄碱、愈创甘油醚、马来酸氯苯那敏、双氯芬酸的含量检测。 相似文献
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A proteomic evaluation of the effects of the pharmaceuticals diclofenac and gemfibrozil on marine mussels (Mytilus spp.): evidence for chronic sublethal effects on stress‐response proteins 下载免费PDF全文
Wiebke Schmidt Louis‐Charles Rainville Gillian McEneff David Sheehan Brian Quinn 《Drug testing and analysis》2014,6(3):210-219
Human pharmaceuticals (e.g. the lipid regulator gemfibrozil and the non‐steroidal anti‐inflammatory drug diclofenac) are an emerging environmental threat in the aquatic environment. This study aimed to evaluate sublethal effects of these two commonly found pharmaceuticals on the protein profiles of marine mussels (Mytilus spp.). Mytilus spp. was exposed to environmentally relevant and elevated concentrations (1 and 1000 µg/l respectively) of both drugs for 14 days. In addition, mussels were maintained for seven days post treatment to examine the potential of blue mussels to recover from such an exposure. Differential protein expression signatures (PES) in the digestive gland of mussels were obtained using two‐dimensional gel electrophoresis after 7, 14, and 21 days of exposure. Twelve spots were significantly increased or decreased by gemfibrozil and/or diclofenac, seven of which were successfully identified by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) analysis. These proteins were involved in energy metabolism, oxidative stress response, protein folding, and immune responses. Changes in the PES over time suggested that mussels were still experiencing oxidative stress for up to seven days post exposure. In addition, a suite of biomarkers comprising glutathione transferase, lipid peroxidation, and DNA damage were studied. An oxidative stress response was confirmed by biomarker responses. To our knowledge, this is the first investigation using proteomics to assess the potential effects of human pharmaceuticals on a non‐target species in an environmentally‐relevant model. The successful application of this proteomic approach supports its potential use in pollution biomonitoring and highlights its ability to aid in the discovery of new biomarkers. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
33.
目的:探讨双氯芬酸钠/盐酸利多卡因治疗中重度偏头痛的临床疗效.方法:将53例偏头痛患者分为两组:双氯芬酸钠/盐酸利多卡因(奥尔芬)治疗组,阿司匹林治疗组.于治疗前后采用视觉模拟疼痛评分(VAS)测定患者疼痛程度进行疗效比较.结果:双氯芬酸钠/盐酸利多卡因治疗组疗效优于阿司匹林治疗组(p<0.01).结论:双氯芬酸钠/盐... 相似文献
34.
目的:观察双氯芬酸钠组与盐酸哌替啶对烧伤植皮患者术后血浆炎症因子水平的影响.方法:临床纳入烧伤植皮患者70例,根据术后镇痛药物的不同分为研究组与对照组.研究组给予双氯芬酸钠,对照组给予盐酸哌替啶.采用疼痛视觉模拟量表(VAS)对两组患者镇痛前后进行疼痛评分.观察两组患者镇痛前后血浆白细胞介素-6、8(IL-6、IL-8)以及肿瘤坏死因子(tumor necrosis factor-α,TNF-α)的水平以及两组患者镇痛期间不良反应发生情况.结果:镇痛前,两组患者疼痛VAS评分无统计学差异(P>0.05).给药1、2、4h后,研究组疼痛VAS评分均明显低于对照组,差异有统计学意义(P<0.05).研究组给药后12 h IL-6、TNF-α水平明显低于对照组(P<0.05);研究组给药后8、12、24 h IL-8水平明显低于对照组(P<0.05).镇痛期间,研究组恶心呕吐以及嗜睡的发生率高于对照组,差异有统计学意义(P<0.05).结论:双氯芬酸钠组用于烧伤植皮患者术后镇痛,起效时间快,能够改善患者血浆炎症因子水平,值得推广. 相似文献
35.
目的:采用差示分光光度法测定双氯灭痛片剂的含量,方法:在波长290nm处,以双氯灭痛在0.01mol/L氢氧化钠溶液为参比液,测其在乙醇中的差示吸收值,结果:双氯灭痛浓度在11~50μg/ml范围内△A与溶液浓度呈良好线性关系(r=0.9996)平均回收率为100.6%,RSD为0.64%,结论:该法简便,快速,准确。 相似文献
36.
Chamomile (Matricaria chamomilla L., Asteraceae) is a medicinal plant widely used as remedy for pain and gastric disorders. The association of non-steroidal anti-inflammatory drugs (NSAIDs) with medicinal plant extracts may increase its antinociceptive activity, permit the use of lower doses and limit side effects. The aim was to isolate and identify the main chemical constituents of Matricaria chamomilla ethanolic extract (MCE) as well as to explore their activity as cyclooxygenase (COX) inhibitors in silico; besides, to examine the interaction between MCE and diclofenac on nociception in the formalin test by isobolographic analysis, and to determine the level of gastric injury in rats. Three terpenoids, α-bisabolol, bisabolol oxide A, and guaiazulene, were isolated and identified by 1H NMR. Docking simulation predicted COX inhibitory activity for those terpenoids. Diclofenac, MCE, or their combinations produced an antinociceptive effect. The sole administration of diclofenac and the highest combined dose diclofenac-MCE produced significant a gastric damage, but that effect was not seen with MCE alone. An isobologram was constructed and the derived theoretical ED35 for the antinociceptive effect was significantly different from the experimental ED35; hence, the interaction between diclofenac and MCE that mediates the antinociceptive effect is synergist. The MCE contains three major terpenoids with plausible COX inhibitory activity in silico, but α-bisabolol showed the highest affinity. Data suggest that the diclofenac-MCE combination can interact at the systemic level in a synergic manner and may have therapeutic advantages for the clinical treatment of inflammatory pain. 相似文献
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目的:探讨双氯芬酸钠栓和布洛芬口服液在儿科临床的退热效果和安全性。方法:100例感染性发热患儿随机分为双氯芬酸栓组和布洛芬组。结果:双氯芬酸钠栓组的起效时间(41.63±3.71)min,短于布洛芬组(56.28±5.23)min,维持正常体温时间(7.31±1.62)h长于布洛芬组(6.29±1.34)h,差异均有统计学意义(P<0.01)。结论:双氯芬酸钠栓对高热患儿具有退热作用快,维持时间长,使用方便、安全,无明显胃肠道反应等优点。 相似文献