Culture-Expanded Autologous Adipose-Derived Mesenchymal Stem Cell Treatment for Osteonecrosis of the Femoral Head

BackgroudOutcomes of traditional treatment for osteonecrosis of the femoral head (ONFH) are not always satisfactory. Hence, cell-supplementation therapy has been attempted to facilitate necrotic-tissue regeneration. Adipose-derived mesenchymal stem cell (ADMSC) transplantation is potentially advantageous over bone marrow-derived MSC implantation, but its outcomes for ONFH remain unclear. The aim of this study was to determine 2-year radiological and clinical outcomes of culture-expanded autologous ADMSC implantation for ONFH.MethodsEighteen hips with necrotic lesions involving ≥ 30% of the femoral head were included. ADMSCs were harvested by liposuction and culture expanded for 3 passages over 3 weeks. With a 6-mm single drilling, ADMSCs were implanted into the necrotic zone. All patients underwent magnetic resonance imaging (MRI), single-photon emission computed tomography/computed tomography (SPECT/CT) at screening and 6 months, 12 months, and 24 months postoperatively. The primary outcome was the change in the size of necrotic area on MRI. Secondary outcomes were changes in clinical scores and radioisotope uptake on SPECT/CT. Conversion total hip arthroplasty (THA) was defined as the endpoint.ResultsPreoperatively, the necrotic lesion extent was 63.0% (38.4%–96.7%) of the femoral head. The mean Harris hip score was 89.2, the University of California at Los Angeles (UCLA) score was 5.6, and Western Ontario and McMaster Universities Arthritis index (WOMAC) was 79.4. Three patients underwent THA and 1 patient died in an accident. Finally, 11 patients (14 hips) were available for ≥ 2-year follow-up. At the last follow-up, no surgery-related complications occurred, and 14 of 17 hips (82%) were able to perform daily activities without THA requirement. There was no significant decrease in lesion size between any 2 intervals on MRI. However, widening of high signal intensity bands on T2-weighted images inside the necrotic lesion was observed in 9 of 14 hips (64%); 11 of 14 hips (79%) showed increased vascularity on SPECT/CT at 2 years postoperatively. No significant differences were observed between preoperative and 24-month mean Harris hip score (89.2 vs. 88.6), WOMAC (79.4 vs. 75.7), and UCLA score (5.6 vs. 6.2).ConclusionsOur outcomes suggest that culture-expanded ADMSC implantation is a viable option for ONFH treatment without adverse events.     

Behavioral and autonomic responses to intermittent social stress: differential protection by clonidine and metoprolol

The present study investigated physiological and pharmacological characteristics of socially stressed animals. Specifically, we examined (1) to what degree autonomic and behavioral stress reactions during intermittent confrontations between an intruder male adult Long-Evans rat with an aggressive resident undergo habituation, and (2) to what extent the defeat-experienced animal can be protected against these stress reactions with clonidine or metoprolol, two adrenergic agents with clinical anxiolytic effects. We developed an acute social stress situation that consisted of initially placing an experimental rat as an intruder into the homecage of a resident while the resident was not present, thereafter permitting brief physical agonistic interactions with the reintroduced resident until the intruder was forced into a submissive supine posture and emitted ultrasonic vocalizations (USV), and eventually exposing the intruder to the resident's threats for one hour, while being shielded from potentially injurious attacks (threat encounter). Over the course of the initial 4-weekly threat encounters the acute tachycardia but not the hyperthermic stress responses decreased in magnitude. Following the first three threat encounters core temperature (T_c) was significantly elevated for at least 3 h. The T_c was already elevated when the repeatedly defeated intruder was confronted with the olfactory cues of the resident's cage. This conditioned anticipatory hyperthermia developed in the course of the first three confrontations and was paralleled by a decrease in exploratory and motor behavior and by an increase in defensive behaviors and in both types of USV emitted in the low (20–30 kHz) and the high (31–70 kHz) frequency range. Clonidine (0.01–0.1 mg/kg, IP), an ₂-adrenergic agonist and metoprolol, a -adrenergic blocker (1.0–10.0 mg/kg, IP), dose-dependently prevented the tachycardic response to stress. Only clonidine, but not metoprolol, also attenuated the rise in T₀ during the 1-h agonistic interaction. Clonidine decreased those aspects of motor behavior (e.g. rearing, walking) that are of lesser cost for the individual but maintained high levels of defensive reactions and increased the duration of low USV. The high doses of clonidine (0.06, 0.1 mg/kg) attenuated the homeostatic regulation and sedated the intruder while exposed to threats during a social confrontation. The absence of attenuation of the high level of defensive behavior and the prolonged low USV suggest a stress intensification by the higher doses of clonidine. In conclusion, after the fourth encounter, the autonomic, behavioral and vocal response pattern prior to and during repeated weekly confrontations show no evidence for habituation for the following 6 weeks. Moreover, adrenergic therapeutic agents that are applied to treat symptoms of anxiety block the tachycardic response but may actually intensify defensive behavior and certain stress vocalizations.     

Profil psychopharmacologique d'un nouvel agoniste Dopaminergique,le RU 24213

The psychopharmacological properties of RU 24213 were compared to those of other dopaminergic agonists (apomorphine, dexamphetamine, bromocriptine and l-dopa) in various behavioural tests. In naive mice the drug reduced the locomotor hyperactivity in the primary exploratory phase and produced stimulation in the subsequent stabilized activity period. In rats it provoked dose-related stereotypies, specially gnawing and sniffing. It delayed the cataleptic state induced by prochlorperazine without affecting its intensity. In animals unilaterally lesioned with 6-OHDA in the nigro-striatal pathway, RU 24213 caused contralateral turning. It exhibited relatively weak emetic and anorexic effects in dogs. Core temperature recordings in rats revealed a biphasic hypo- and hyperthermic activity. In drug interaction studies it was observed that stereotypies and rotations induced by RU 24213 were blocked by haloperidol and decreased by MT. Reserpine respectively decreased stereotypies and increased ipsilateral rotations in rats with unilateral electrolytical striatal lesion.The results obtained suggest that RU 24213 stimulates dopamine receptors both directly and indirectly. In this respect it could be compared to bromocriptine but unlike this latter compound it has an immediate effect which is of shorter duration.

     

人才建设是构建军队医院核心竞争力的根本保障

核心竞争力属于知识范畴,而人才是知识的载体.文章在对12所军队医院及其特色科室人才情况调研的基础上,提出人才建设是军队医院构建核心竞争力的根本保障,必须优化人才结构,建立合理人才梯队,创新人才机制,如科主任管理机制、人才使用机制、人才培养机制和人才组合机制等.     

知识型县级医院核心人才素质的立体模糊评估

在构建知识型县级医院核心人才素质评价指标体系的基础上。综合运用模糊数学、概率论、空间两点距离等理论进行核心人才素质立体模糊量化评估．帮助县级医院有效鉴别核心人才，促进县级医院人力资源管理工作和县级医院发展。     

生物医学核心期刊《中华医院感染学杂志》5年相关文献信息统计分析和质量评价

目的探索生物医学核心期刊《中华医院感染学杂志》5年相关文献信息质量本质。方法选择该杂志2000~2004年48期2 612篇论文作为调查统计源,分析评价相关文献信息质量。结果5年载文信息量平均指数为0.57;作者来源以医科大学和附属医院、省、地(市)和部队医疗卫生及科研单位为主;论文以研究类、论著类和经验总结类为主;5年基金课题共160项;5年平均每篇论文引文6.0条,中文占68.6%,外文占31.4%,期刊自引占34.2%;5年论文出版时滞平均为273.5 d。结论上述结果的6个方面在全国生物医学期刊中都具有相当的优势,凸显了该刊“核心期刊”的质量内涵。     

医学院校法学课程教学方法探讨

现行医学院校法学课程教学方法存在许多不足,应该建立以课堂理论讲授为基础,采用多种教学手段传授学生法律知识的教学方法体系,其中具有基础性地位和方法论意义的是课堂讲授教学法和课堂讨论教学法。     

髓芯减压加缝匠肌骨瓣移植术在治疗股骨头坏死中的价值

目的:观察髓芯减压加缝匠肌骨瓣移植术治疗股骨头坏死的疗效。方法:对65例股骨头坏死患者,采用髓芯减压加缝匠肌骨瓣移植术治疗。结果:经28个月(10-56个月)随访,疗效评价,优38髋,良21髋,可6髋,差0髋。优良率>90%。结论:髓芯减压加缝匠肌骨瓣移植术治疗股骨头坏死,效果良好。     

“大学生野外生存生活训练“课程实验研究分析

本文通过对课题实验数据分析,结果表明大学生野外生存生活训练作为新兴的体育课程,对提高大学生生存生活能力,提高环境适应能力,提高学生身心素质,培养学生终生体育意识,激发学生学习动机,培养学生团队合作精神,挖掘学生潜能,发展学生个性,拓展高校体育课的时间和空间,深化体育教学改革,具有良好的教学效果和社会效益,具有普及和推广的价值。