Interaction of immunoglobulin with actin

Actin can form specific, direct associations with immunoglobulin resulting in soluble complexes or cross-linked matrices. This interaction can be detected by four in vitro assays using purified components: (1) actin enhances the cytophilic activity of guinea pig IgG2; (2) in solutions of low ionic strength, actin and IgG2 co-precipitate: (3) soluble complexes exist in 0.1 M KCl as revealed by the displacement of actin from its expected sedimentation pattern in a gradient of sucrose when in the presence of IgG 1, IgG2, or IgM; (4) immunoglobulin (IgG1, IgG2, BGG)‡: increases the viscosity of F-actin solutions, presumably by crosslinking F-actin filaments. These data suggest that direct interaction of a cytoskeletal protein with a cell surface receptor is possible.     

β Chain deficiency in three patients with dysfunctional C8 molecules

Structural and functional studies were performed on a dysfunctional C8 molecule present in the serum of two siblings and an unrelated individual. The C8 in these three sera exhibited a pattern of partial immunologic identity with C8 in normal serum but was devoid of functional activity. The C8 was immunoprecipitated from the three sera and from a control serum with an antihuman C8 antiserum and analyzed by SDS-PAGE using highly purified human C8 as a reference. A selective absence of a band of 62,000 mol. wt was observed in the immunoprecipitates from the sera containing dysfunctional C8. Experiments performed with the purified α-γ and γ subunits showed that the hemolytic activity of the C8 deficient sera could be reconstituted by the addition of the β chain but not the α-γ dimer. Binding of the dysfunctional C8 to C$\text{567}$ was excluded by the following observations: (1) EAC 1–7 treated with the C8 deficient sera and then washed could not be lysed after the addition of the β subunit and C9; and (2) the abnormal molecules did not interfere with the consumption of normal C8 by the soluble complex SC5b-7.     

Ultracytochemical studies of the blood-meningeal barrier (BMB) in rat spinal cord

Summary Alkaline phosphatase(AP),5-nucleotidase(5N) and nucleoside diphosphatase (NDPase) activities were studied by cytochemical methods applied to light and electron microscopy in the microvasculature of spinal cord leptomeningeal strips of normal and protamine sulfate (PS) treated rats. The increased permeability to intravenously injected horseradish peroxidase was observed in some segments of microvessels of PS treated rats. Enhanced formation of plasmalemmal pits and deep invaginations, formation of numerous pinocytic vesicles and the appearance of channel-like structures in the cytoplasm of endothelial cells were the most striking ultrastructural evidence of increased permeability of the affected microvessels. All of these structures also showed activity of AP, and to lesser extent, of NDPase; 5N activity was mainly associated with the delimiting membranes of pinocytic vesicles. Our data present evidence that a shift of enzymatic activity from luminal to abluminal surface of affected endothelial cells results from membrane flow accompanying increased transport activity via formation of pinocytic vesicles and channel-like structures.Supported in part by a grant from NINCDS No. 17271-01Visiting scientist from the Neurological Institute of the University of Vienna, Vienna, Austria     

罗通定中降解物巴马汀限量检测方法

目的：研究解决罗通定及硫酸罗通定注射液中降解物巴马汀的检测方法。方法：用薄层色谱法和紫外分光光度法限量检查样品中的巴马汀。结果：与对照品斑点大小、荧光强度比较和用吸收度限值控制均可对样品中的巴马汀进行限量检查，结果与原标准一致。结论：两方法均灵敏，专属，简便，快速。     

安宝治疗早产的临床研究

目的　探讨 β2 -肾上腺素能受体兴奋剂安宝抑制子宫收缩 ,治疗早产的临床效果。方法　对 91例先兆早产孕妇随机分为两组 ,分别静脉滴注安宝或硫酸镁。采用达到有效宫缩的最低浓度 ,适时停药 ,病情反复则间歇多次应用。结果　安宝首次用药达有效抑制宫缩的显效时间为 2h 40min ;延长孕期 2 9.3 4d ;足月分娩率 71.74% ;均极显著优于硫酸镁组 (P分别 <0 .0 0 1　 0 .0 1　 0 .0 1)。尚未发现严重毒副反应。结论　安宝抑制宫缩作用强显效快 ,治疗早产安全有效。     

镁离子对脑保护作用的前瞻性临床实验研究

目的探讨分析镁制剂在重型颅脑损伤临床治疗中的实用价值.方法对76例重型颅脑损伤患者按随机方法分成实验组和对照组,对照组按常规方法治疗,实验组在常规治疗的基础上加用大剂量硫酸镁.治疗后从颅内压、NSE、CT、GCS评分和G0S评级等几个方面进行统计分析.结果实验组与对照组比较,颅内压和NSE水平明显下降,CT显示脑水肿程度减轻,而GCS和G0S则有明显提高,经统计学检验有显著性差异(P＜0.01和P＜0.05).结论镁制剂对重型颅脑损伤治疗确有实用价值,且经济、安全,值得推广.     

高频振荡通气及联合硫酸镁治疗新生猪重症胎粪吸入综合征的实验研究

目的　探讨高频振荡通气 (HFOV)及联合硫酸镁 (Mg SO4 )治疗合并持续肺动脉高压(PPH)的重症胎粪吸入综合征 (MAS)模型氧合、循环功能 ,血镁浓度及肺组织病理改变。　方法　以2 0 %胎粪混悬液制备重症 MAS模型 ,健康新生猪随机分为 3组 ,即模型 HFOV治疗组 (HFOV组 ,n= 6 ) ,HFOV+Mg SO4 治疗组 (HFOV+Mg SO4 组 ,n=7) ,HFOV对照组 (对照组 ,n=5 ) ,HFOV+Mg SO4 组同时静脉持续泵入 Mg SO4 。监测生命体征、血气、血镁浓度。　结果　 (1) HFOV和 HFOV+Mg SO4 治疗均使 MAS模型动脉血氧分压 (Pa O2 )、动脉血氧 /肺泡血氧分压比 (a/ APO2 )增加 ,肺泡-动脉血氧分压差 (A- a DO2 )、肺内分流 (Qs/ Qt)降低 ,治疗 30 min与治疗前比差异有非常显著性 (P<0 .0 1)。HFOV组各时间点 Pa O2 、a/ APO2 低于对照组 ,A- a DO2 、Qs/ Qt高于对照组 (P<0 .0 5 )。HFOV+Mg SO4 组治疗 12 0 m in后上述指标与对照组差异无显著性 (P>0 .0 5 )。 (2 )尽管氧合功能改善 ,单独 HFOV对重症 MAS的 PPH无降低作用 ,联合 Mg SO4 治疗 30 min即可有效降低 PPH(P<0 .0 5 ) ,并保持疗效。(3) HFOV组较 HFOV+Mg SO4 组有明显肺出血 ,出血沿肺段、小叶分布 ,两组病理评分差异有显著性 (P<0 .0 5 )。 (4) HFOV+Mg SO4 组血镁浓度较治疗     

Transport and uptake characteristics of a new derivative of berberine(CPU-86017) by human intestinal epithelial cell line: Caco-2

AIM: The characteristics of transepithelial transport and uptake of CPU-86017 {[7-(4-chlorbenzyl)-7,8,13,13α-tetrahydroberberine chloride, CTHB]}, a new antiarrhythmia agent and a new derivative of berberine, were investigated on epithelial cell line (Caco-2) to further understand the absorption mechanism of berberine and its derivatives. METHODS: Caco-2 cell was used. RESULTS: 1) The permeability coefficient from the apical (AP) to basolateral (BL) of CPU-86017 was approximately 5 times higher than that from BL-to-AP transport. The effects of a P-glycoprotein (P-gp) inhibitor-cyclosporin A, some surfactants, and lower pH on the transepithelial transport of CPU-86017 were also observed. Cyclosporine A at 7.5 mg/L had no effect on the transepithelial electrical resistance (TEER); an about 4-fold enhancement on the transepithlial transport of CPU-86017 was observed. Some surfactants (sodium citrate, sodium deoxycholate, and sodium dodecyl sulfate) at 100 μmol/L and low pH (pH=6.0) induced a reversible d