RP-HPLC法测定马来酸桂哌齐特片中有关物质的含量

目的:建立以反相高效液相色谱法测定马来酸桂哌齐特片中有关物质含量的方法。方法:色谱柱为Hypersil ODS2,流动相为0.05mol·L-1磷酸氢二钠溶液(用磷酸调pH至4.5)-乙腈(75∶25),检测波长为230nm,流速为1.0mL·min-1,进样量为20μL。结果:有关物质与主成分分离良好,3批样品中有关物质含量均为0.1%。结论:本方法操作简单、专属性强,可用于该制剂中有关物质的含量测定。     

HPLC测定复方新斯的明眼用凝胶中马来酸氯苯那敏的含量和有关物质

目的建立马来酸氯苯那敏及有关物质的含量测定方法。方法采用固相萃取-高效液相色谱法,EclipseXDB-C18色谱柱（4.6mm×150mm,5μm）。流动相：甲醇-醋酸三乙胺溶液（45∶55）。检测波长：261nm。结果线性范围为1.02～16.32μg·mL^-1,r=0.9999。低、中、高3个浓度的平均回收率为（103.05±1.24）%,（100.87±1.45）%,（99.63±1.03）%。结论本方法简便快速、专属性好、重复性好、易操作。     

HPLC-ELSD测定威灵仙中齐墩果酸的含量

目的建立威灵仙中齐墩果酸的高效液相色谱-蒸发光散射法测定方法。方法采用SumFire ^TMC18（4.6mm×250mm,5μm）色谱柱,柱温40℃;乙腈-水（85∶15）为流动相,流速1.0mL·min^-1;蒸发光散射检测器漂移管温度85℃,空气流速2.3L·min^-1。结果齐墩果酸在0.214～1.92μg内线性关系良好,r=0.9999,平均回收率为98.8%,RSD为0.9%（n=6）。结论该方法简便,快速,准确,可作为威灵仙的质量控制。     

Effect of the drug-matrix on the stability of enalapril maleate in tablet formulations

The chemical stability of enalapril maleate in tablet dosage forms consisting of different formulation excipients has been studied in this work. The influence of various parameters such as heat, moisture, light and the drug-matrix was investigated. The degradation of enalapril maleate has been followed by using an HPLC method, which was demonstrated to be specific, stability indicating, accurate and precise. The degradation kinetics of enalalpril maleate in phosphate buffer solutions of pH values in the range of 2.2–10.5 were observed to be psuedo first order throughout the whole pH range studied. Enalapril maleate alone showed high stability for temperature under dry and humid conditions, however it became unstable when mixed with the drug-matrix in its tablet formulations and exposed to the same conditions. The pathway of degradation of enalapril maleate was found to be pH dependent. The extent of degradation of two different enalapril maleate tablet formulations (product A of a basic drug-matrix and product B of an acidic drug-matrix) has been investigated. The degree of degradation of the product with acidic matrix was significantly less than that of the basic matrix under same temperature and humidity conditions. Infact, diketopiperazine and enalaprilat degradants were mainly associated with the degradation of the product with the acidic matrix and that with the basic matrix, respectively. Dry enalapril maleate powder showed some photolysis, which was more significant with daylight (3.3%) compared with that under UV light (0.2%). Although the product with the acidic matrix showed some photolysis but the effect was not pronounced and the % recovery of enalapril was almost complete and within the acceptable experimental errors. However, the product with the basic matrix showed almost no response for photolysis.     

反相高效液相色谱法测定甲麻芩苷那敏片中马来酸氯苯那敏含量

目的用反相高效液相色谱(RP-HPLC)法测定甲麻芩苷那敏片的马来酸氯苯那敏含量。方法色谱柱为岛津C18柱(250mm×4.6mm,5μm),流动相为0.05mol/L磷酸二氢钾(含1%三乙胺,用磷酸调节pH=3)-0.005mol/L己烷磺酸钠甲醇溶液(1.2∶1),流速为1mL/min,紫外检测波长为223nm,柱温为30℃,进样量为20μL。结果马来酸氯苯那敏质量浓度在5.49～71.32μg/mL范围内与峰面积呈良好的线性关系,平均回收率为99.07%,RSD为1.64%。结论RP-HPLC法简便、准确,可作为甲麻芩苷那敏片的含量测定方法。     

HPLC法测定氨咖黄敏胶囊中对乙酰氨基酚、咖啡因、马来酸氯苯那敏的含量

目的:建立HPLC法测定氨咖黄敏胶囊中对乙酰氨基酚、咖啡因和马来酸氯苯那敏的含量;方法:色谱柱:Kromasil苯基柱(250 mm×4.6 mm,5μm);流动相:乙腈-水-三乙氨(20:79:1)(磷酸调pH至3.1),检测波长为260 nm;结果:线性范围:对乙酰氨基酚15.13～35.30μg,r=0.9999;咖啡因0.911～2.11μg,r=1.0000;马来酸氯苯那敏0.06～0.15μg,r= 0.9998;平均回收率:对乙酰氨基酚99.5%,RSD%=0.5%;咖啡因100.0%,RSD%=0.3%;马来酸氯苯那敏99.6%,RSD%=0.9%。结论:本方法简便、准确、灵敏度高、重现性好,可更好地控制本品质量。     

HPLC法测定甲麻芩苷那敏片中三组分含量

建立反相离子对HPLC法测定甲麻芩苷那敏片中三组分的含量。采用Diamonsil C18色谱柱,以[0.2%辛烷磺酸钠溶液-三乙胺(100∶0.2,用磷酸调节pH值为4.3)]-甲醇(50∶50)和(40∶60)为流动相,分别测定盐酸甲麻黄碱、黄芩苷及马来酸氯苯那敏,检测波长为215nm,流速为1.0 mL.min-1。盐酸甲麻黄碱、黄芩苷及马来酸氯苯那敏的线性范围分别为16~125μg.mL-1(r=0.9999)、11~85μg.mL-1(r=1.000)及26~102μg.mL-1(r=0.9991),平均回收率分别为99.7%(RSD=1.1%)、99.4%(RSD=1.1%)及100.2%(RSD=1.6%)。本方法简单、迅速、准确,可用于甲麻芩苷那敏片中三组分的含量测定。     

HPLC法测定复方岩白菜素片中马来酸氯苯那敏的含量

采用高效液相色谱法测定复方岩白菜素片中马来酸氯苯那敏的含量。色谱柱为DiamonsilC18,流动相:乙腈-0·3%十二烷基硫酸钠-磷酸(60∶40∶0·02),用三乙胺调pH值为4·5,流速1·0mL·min-1,检测波长:210nm,马来酸氯苯那敏在0·949~7·592μg·mL-1范围内呈良好线性关系(r=0·9999),回收率为99·01%(n=9),RSD为0·20%。     

马来酸三甲氧苯丁氨酯的镇痛作用与单胺类递质的关系

探讨马来酸三甲氧苯丁氨酯 (TM )镇痛作用与单胺类递质的关系 .小鼠扭体法及热板法实验的结果表明 ,利血平和赛庚啶能够减弱TM的镇痛作用 ,L 色氨酸则增强其镇痛作用 .酚妥拉明、普萘洛尔、二乙基二硫代氨基甲酸钠、阿朴吗啡和氟哌啶醇对其镇痛作用无明显影响 .应用荧光法测定小鼠脑内单胺类递质含量 ,结果表明腹腔注射TM 70mg/kg可提高小鼠脑内 5 HT含量 ,对NE和DA含量无明显影响 .说明TM的镇痛作用与中枢 5 HT系统有关     

HPLC法测定复方氨肽素片中马来酸氯苯那敏的含量及含量均匀度

目的:建立HPLC法测定复方氨肽素片中马来酸氯苯那敏的含量及含量均匀度。方法:色谱柱为Welch Material XB-C18(4.6 mm×150 mm,5μm);流动相为0.05 mol.L-1磷酸二氢钠溶液(用三乙胺调节pH值至3.0±0.1)-甲醇(60∶40);检测波长265 nm。结果:马来酸氯苯那敏进样量在0.107 7～0.646 2μg范围内与峰面积线性关系良好(r=1.000 0);平均回收率为99.06%,98.83%,99.14%;RSD为0.93%,0.57%,0.46%(n=3);精密度、重现性良好。结论:所建方法快速准确,灵敏度高,结果可靠,为本品质量标准的修订和提高提供了基础。