Chlamydia trachomatis serology in ankylosing spondylitis

Summary Demonstration of chlamydial antibodies in patients with ankylosing spondylitis (AS) could show an etiological roel of Chlamydia trachomatis in this condition. We studied serum specimens from 50 HLA-B27 positive patients with AS (Group I), 34 HLA-B27 positive patients with other rheumatic diseases (Group II), 67 HLA-B27 positive healthy blood donors (Group III) and 37 healthy untyped blood donors. (Group IV). Measured by an immunoperoxidase assay (IPA) chlamydial IgA (titre 120) was more prevalent in the HLA-B27 positive persons than in the healthy controls not selected for HLA-group (Groups I+II+III vs IV: p<0.02). Chlamydia trachomatis IgA-IPA containing sera also had specific IgG-IPA antibodies (180) in 29 (96%) out of 30 sera from HLA-B27 positive individuals and controls. Conversely, 45% of specific IgG-positive (180) AS sera, 27.7% sera in Group II, 39.4% Group III sera vs. 11.1% of sera in Group IV had concomitant chlamydial IgA (120). The differences in the prevalence of specific IgA were statistically significant: Group I vs. IV: p<0.01; Group III vs. IV: p<0.05 and Gr. I+II+III vs. IV: p<0.05. Our data suggest an enhanced antibody production against Chlamydia trachomatis among the HLA-B27 positive individuals whether they have AS or are healthy.     

阿奇霉素配伍干扰素治疗女性生殖道支原体衣原体感染的疗效观察

目的 探讨女性生殖道支原体、衣原体感染治疗的最佳方法.方法 将120例患者随机分为治疗组(56例)和对照组(64例).治疗组每天静脉点滴乳酸阿奇霉素,7天为1个疗程,同时干扰素栓阴道给药,隔日1次,7次为1个疗程.对照组静脉用药同治疗组.结果 对照组有效率75.0%,治疗组有效率达89.3%,两组比较有显著性差异(P＜0.05).结论 阿奇霉素配伍干扰素栓治疗女性生殖器道支原体、衣原体感染优于单一静脉用药.     

沙眼衣原体感染对精子数量及质量的影响

目的探讨男性生殖道沙眼衣原体(CT)感染对精液分析各参数产生的影响。方法CT采用抗原测试方式进行检测,精液分析采用计算机辅助精子分析系统进行测试。对测试结果进行统计学分析。结果精子密度感染组明显低于对照组(P<0.01);精子活动率指标a级精子率、b级精子率、c级精子率、d级精子率对照组明显好于感染组(P<0.05),精子活动力评估项目曲线速度、直线速度、平均路径速度、平均移动角度对照组优于感染组(P<0.01)。结论CT感染可引起精子数量减少、运动质量和能力减弱,进一步证实了生殖道CT感染是男性不育的重要原因。     

荧光定量聚合酶链反应检测女性生殖道分泌物沙眼衣原体等病原体

目的:检测女性生殖道分泌物中淋病奈瑟菌(NG)、沙眼衣原体(CT)、解脲脲原体(UU)的数量,以了解本地区上述3种病原体感染现状。方法:用荧光定量聚合酶链反应(FQ—PCR)检测了121份标本的NG—DNA和149份标本的CT—DNA与UU—DNA。结果:NG阳性率为49.6％(60/121),其阳性标本的平均拷贝数为3.61×10~5拷贝/ml;CT阳性率为23.5％(35/149),其阳性标本的平均拷贝数为9.59×10~4拷贝/ml;UU阳性率为34.3％(51/149),其阳性标本的平均拷贝数为4.11×10~5拷贝/ml。结论:FQ—PCR具有简便、快速、准确的优点,是目前快速诊断淋菌、非淋菌性阴道炎可靠准确的方法。     

1593例妇科病人沙眼衣原体、支原体和淋球菌的检测报告

对１５９３例阴道炎、宫颈炎、盆腔炎等妇科疾患者进行衣原体，支原体，淋球菌检测。结果衣原体８３例，解脲脲原体７４２例，人型支原体７６例，淋球菌２００例，共１１０１例，占被检人数的６９．１％。其中，两种及两种以上病原体混合感染１９２例，占感染病例中的１７．４％。结果表明：４种病原体在女性患者中的感染率相当高，应引起极大关注，采取有效措施，控制其传播。     

Detection of Chlamydia trachomatis in Papanicolaou-stained cervical smears: control study by in situ hybridization

Ninety-one Papanicolaou-stained vaginopancervical smears were destained and subjected to in situ hybridization with Chlamydia trachomatis DNA probe. At cytologic examination (Pap test), 71 smears showed changes suggestive of chlamydial infection, while remaining 20 were negative. At the control by in situ hybridization, the results of Pap test were confirmed in 85 out of 91 cases, two false-positive and four false-negative cases being detected. The sensitivity and specificity of Pap test, compared with in situ hybridization, were 95% and 89%, respectively. Like some recent reports, the present study confirms the reliability of Pap test in the detection of Chlamydia trachomatis and its possible relevant role in reducing the diffusion of the infection, when properly applied to mass-screening program.     

生殖道沙眼衣原体感染66例分析

应用标记荧光素的抗沙眼衣原体（Ct）单克隆抗体，以直接免疫荧光法（DFA）检测66例生殖道标本，Ct感染率为12．12％，淋菌感染率为1．5％。结果显示西宁存在生殖道的Ct感染，故应引起高度重视。     

The diagnosis of chlamydial infection in a cytology laboratory: ten months' experience using immunofluorescence with and without previous cytologic prediction

A Chlamydia diagnostic service has been established as part of a cytology laboratory, combining prediction of possible infection by routine cytology with definitive testing by immunofluorescence. The first 10 mo of operation have had good clinician acceptance with 527 specimens submitted and 128 reported as positive (24.3%). Routine cytology was more accurate in predicting chlamydial infection than was clinical evaluation (32.8% of cytologically suspected cases were positive versus 20.2% of clinically suspected cases); however, both failed to identify many infected women. The patient selection for testing must be improved since only about 22% of infected women in our population were tested. Nevertheless, the service identified a large number of patients infected with an important sexually transmitted pathogen. Chlamydial diagnosis has become a major component of the laboratory's workload and has been cost-effective.     

The nature of urogenital involvements in female uro-arthritis,with special reference to chlamydial infection

Summary To study the nature of urogenital involvements in female uro-arthritis 73 consecutive patients with arthritis concomitant with any type of urogenital involvement were examined. The controls were 281 females interviewed only and an additional 83 also gynaecologically examined. A history of cervicitis, salpingitis, dysuria and pyelocystitis/-nephritis occurred significantly more often in patients than in controls. Clinical gynaecological examination revealed cervicitis in 26,8 % (19/71) of the patients and 15.7 % (13/83) of the controls (p<0.05). Aseptic pyuria was definitely more frequent in patients (19/73) than in controls (0/63). The isolation of Chlamydia trachomatis was positive in 14.7 % (10/68) of the patients and 3.7 % (3/81) of the controls (p<0.025). Serological evidence (titre 64) for chlamydial infection was obtained in 53.4 % (39/73) of the patients and 18.2 % (14/77) of the controls (p< 0.00025). The results indicate the importance of urogenital history and findings in females with rheumatic attacks. The most prominent and persistent urogenital involvements were cervicitis, salpingitis, pyuria and dysuria. Chlamydial infection appears in any case to be responsible for part of these involvements (42/73).     

支原体和衣原体生殖道感染认知水平的现状调查与对策

目的：了解人群对支原体和衣原体生殖道感染的认知程度,为设计规范的健康教育内容提供依据。方法;采用自行设计的“支原体和衣原体的生殖道感染相关知识问卷调查表”对192例来我院妇计科门诊就诊的患者进行了问卷调查。结果;调查对象对支原体和衣原体生殖道感染的传播途径知晓率较低,对“推迟首次性交年龄能预防支原体感染”和“感染后的严重并发症有宫外孕”持否定态度的占绝大多数,对“感染后临床表现重”持肯定态度的占大多数。结论;大多数调查对象缺乏足够的支原体和衣原体生殖道感染知识,建立规范的健康教育很有必要。