三味中药配伍应用对改善烫伤小鼠受损免疫功能的作用

目的　观察对烫伤小鼠受损免疫功能具有调理作用的单味中草药 ,经过配伍应用后的协同作用。方法　采用 1 .0 3 4× 1 0 5 Pa的蒸气烫伤 1 0s ,烫伤面积 1 6% ,深度为Ⅲ度的致伤模型烫伤动物。通过观察MΦ的吞噬功能、枯否细胞清除能力、MΦ抗原提呈功能、血清TNF量、淋巴细胞增殖反应及IL 2产生能力、小鼠抗体产生能力、PMNH2 O2 释放量及PMN溶菌酶释放量、血清补体的趋化作用和聚集作用等指标 ,籍以了解单味中药与配伍中药对烧 (烫 )伤小鼠受损免疫功能的调理作用。结果　单味中药三七(PP) ,金银花 (LJ)和虎杖 (PC)对小鼠受损免疫功能有一定的改善作用。PC +LJ、PP +PC、PP +LJ等二味配伍的中药 ,除少数指标外 ,均比单味药的作用好。而PP +LJ+PC三味配伍药的调理作用 ,比单味药和二味配伍药的调理作用更佳。结论　单味中药经配伍应用后对烧 (烫 )伤小鼠严重受损的免疫系统功能 ,具有比单味药更好的协同调理作用。     

中药复方962对老龄大鼠皮质胆碱乙酰转移酶和乙酰胆碱酯酶活性的影响

为研究中药复方 962胶囊对老龄大鼠皮质胆碱乙酰转移酶 (ChAT)和乙酰胆碱酯酶 (AchE)活性的影响 ,将2 2月龄和 2 4月龄Wistar大鼠分为青年对照组、老年模型组、阳性对照脑复康组 ( 0 .56g/kg)、962中剂量组 ( 0 .9g/kg)和 962大剂量组 ( 1 .8g/kg) 5组 ,分别灌胃给药 1～ 2个月后处死大鼠。用Fonnun法和羟胺比色法分别测定 5组大鼠皮质的ChAT和AchE的活性。结果 :中药复方 962大剂量 ( 1 .8g/kg)对老龄大鼠皮质ChAT活性有提高作用 (P <0 .0 5) ,但对AchE无显著影响。提示 :中药复方 962可能通过影响老龄大鼠中枢胆碱能神经系统而改善其衰老的变化     

一氧化氮内皮素-1与早期糖尿病肾病及中医药防治

近年发现一氧化氮(NO)和内皮素-1(ET-1)很可能构成一对具有拮抗作用的血管活性物质,在糖尿病肾病1期肾小球高滤过中起重要的调控作用。而中药对糖尿病早期异常肾血流动力学的改善作用的研究报告很少,且目前有关研究似显肤浅。     

中药肛滴在治疗消化道恶性肿瘤中的应用

探讨中药肛滴在治疗消化道恶性肿瘤中的应用。通过对６例典型病例的回顾分析,观察中药肛滴在缓解癌性粘连性肠梗阻、缓解消化道恶性肿瘤患者症状、提高晚期消化道恶性肿瘤患者生存质量的作用。应用中医理论“通则不痛”之说,对消化道恶性肿瘤患者进行中药肛滴治疗．认为有较好的临床效果。     

Novel stop-gain RNF170 variation detected in a Chinese family with adolescent-onset hereditary spastic paraplegia

Hereditary spastic paraplegia (HSP) is a heterogeneous group of inherited neurodegenerative disease characterized by progressive lower limb spasticity. Recent studies revealed that biallelic variants in RNF170 gene cause autosomal recessive complicated HSP with infancy onset. Here, we report an adolescent-onset HSP patient from a consanguineous Chinese family, with lower extremity stiffness, spastic gait, and unstable straight-line walking as the main manifestations. Whole-exome sequencing identifies a novel RNF170 mutation c.190C>T (p.R64*), which co-segregates with the disease in this pedigree. Functional analysis, including quantitative real-time PCR (RT-qPCR) and Western blot, indicates that both the mRNA and protein levels of mutant RNF170 are significantly reduced, which confirms the loss-of-function mechanism. Our study expands the spectrum of RNF170-associated HSP, while the RNF170 protein-involved degradation of the inositol 1,4,5-trisphosphate receptor in neurodegenerative motor neuron disorders deserves further investigation.     

中药材“地区习惯用药”渐趋分化论

论述了中药材“地区习惯用药”的由来与含义,近现代和历史上的“地区习惯用药”的实例以及产生“地区习惯用药”的原因,随品种性质判明而渐趋分化等。最后提出应该积极研究,促其分化,按其本质分类处理的建议。     

Modulation of agonist binding by guanine nucleotides in CHO cells expressing muscarinic m1 – m5 receptors

In membranes prepared from CHO-m2 cells, inhibition of [³H]-N-methylscopolamine ([³H]NMS) binding by several muscarinic agonists resulted in competition curves with Hill slopes significantly different from unity. Addition of 5-guanylylimidodiphosphate (Gpp(NH)p) led to an increase in the IC₅₀ value of the agonists with significant steepening of the inhibition curves. The shift in potency induced by Gpp(NH)p differed among the agonists with a rank order of oxotremorine-M = carbachol > oxotremorine > McN-A-343 = pilocarpine. In CHO-m4 membranes, Gpp(NH)p was less efficacious than in CHO-m2 membranes whereas no effect of the guanine nucleotide was found in membranes prepared from CHO-m1, -m3, and-m5 cells. No major differences in the effect of Gpp(NH)p among agonists were found in CHO-m4 cells. Atropine binding was not affected by the guanine nucleotide. Together, these results indicate that coupling of G-proteins to muscarinic receptors linked to inhibition of cyclic adenosine monophosphate (cAMP) (m2 and m4) but not of those linked to phosphoinositol turnover (m1, m3 and m5) can be perturbed by Gpp(NH)p. The differential effects observed with Gpp(NH)p between agonist binding to m2 and m4 receptors appear to be receptor-specific and may reflect differences in the G proteins activated by these receptors in CHO cells.     

预防医学在医院管理中的应用

本文针对目前一些医院在发挥预防功能方面存在“预防为主”意识不强、预防专业人员匮乏、管理不力和投入少等不足之处,进而从增强“预防为主”的意识、健全预防管理组织、搞好三级预防等方面提出了自已的做法和建议。