二氧化硫气体对小鼠肺组织细胞周期的影响

目的:检测小鼠吸入二氧化硫(SO2)慢性中毒后肺组织细胞周期的变化.方法:以昆明种小鼠作为实验对象,设计SO2 3个剂量组和1个阴性对照组,通过动式吸入染毒,于染毒6个月后,采用流式细胞仪检测肺组织的细胞周期.结果:各剂量组细胞周期中G0/G1(%)随染毒剂量的增加而减少,且各组间G0/G1(%)有显著性差异(P＜0.05),其中高剂量组与阴性对照组比较,有显著性差异(P＜0.01);各剂量组S(%)随染毒剂量的增加而增高,各剂量组S%有显著性差异(P＜0.01),其中高、中剂量组与阴性对照组比较,均有显著性差异(P＜0.01);G2/M(%)随染毒剂量的增加而增高,且各组间G2/M(%)有显著性差异,其中高剂量组与阴性对照组比较,有显著性差异(P＜0.01).结论:小鼠吸入SO2慢性中毒后,在细胞周期中,G0/G1期细胞所占百分数随染毒剂量的增加而减少,而S期与G2/M期细胞所占百分数增高,提示小鼠肺组织细胞处于较高的增殖状态.     

喉癌病变过程中细胞周期调控因子的流式检测及意义

目的：探讨细胞周期调控基因cyclin E、cyclin D1、p21、p16在喉癌发生、发展中的作用。方法：选取23例声带息肉、69例喉癌前病变、33例喉癌石蜡包埋组织为研究对象,切片脱蜡后制备成细胞悬液,采用流式细胞术检测cyclin E、cyclin D1、p21、p16基因的荧光表达情况。结果：在声带息肉、喉癌前病变、喉癌组织中,cyclin E的阳性表达率分别为13.04%、20.29%和42.42%;cyclin D1的阳性表达率分别为26.09%、43.48%和93.94%;p16的阳性表达率分别为61.90%、40.98%和14.28%;p21的阳性表达率分别为47.62%、23.81%和26.23%。提示cyclin D1、cyclin E的阳性率表达形成声带息肉→喉癌前病变→喉癌组织阳性强度逐渐增强的梯度现象,而p21、p16的阳性表达强度则呈逐渐减弱的现象。结论：①细胞周期调控因子的异常表达是喉癌发生的分子事件;②正性调控因子cyclin D1、cyclin E高表达,负性调控因子p16、p21低表达,即多个与细胞周期相关的正负调控因子失衡在喉癌的发生中可能起着重要作用。     

Localization of the glutamine transporter SNAT1 in rat cerebral cortex and neighboring structures, with a note on its localization in human cortex

SNAT1 mediates glutamine (Gln) influx into neurons and is believed to replenish the transmitters pools of glutamate (Glu) and gamma-aminobutyric acid (GABA). We investigated its distribution and cellular localization in the cerebral cortex and neighboring regions of rats and humans using light and electron microscopic immunocytochemical methods with specific antibodies. In the first somatic sensory cortex of rats and in areas 9, 10, 21 and 46 of the human cortex, numerous SNAT1-positive (+) cells were present in the cortical parenchyma and in the white matter; >95% of SNAT1+ cells were neurons, but some were astrocytes. Most SNAT1+ cells were pyramidal neurons, but numerous non-pyramidal neurons were also observed: SNAT1/GABA double-labeling studies showed that SNAT1 is expressed in all GABA+ neurons. SNAT1/synaptophysin studies showed that <0.1% of all synaptophysin+ puncta coexpressed SNAT1. SNAT1 immunoreactivity (ir) was also in leptomeninges, ependymal cells and choroid plexus. Electron microscopic studies showed that neuronal SNAT1 ir was almost exclusively observed in perikarya and dendritic profiles. SNAT1 ir was also in distal astrocytic processes, including end feet profiles, and in leptomeninges. These findings suggest that the major function of SNAT1 is not to replenish the transmitter pools of Glu and GABA.     

运用PDCA循环改进居家养老护理文件书写质量

目的运用PDCA循环的质量管理方法,改进居家养老护理文件书写质量。方法对现存的护理质量不合格项目进行原因分析、制定改进计划、组织实施、效果评价。结果改进后的居家养老护理文件书写合格率达到98%。结论通过应用PDCA循环质量管理工具进行护理质量管理,使该中心居家养老护理文件书写水平得到大幅度提高,同时促进了该中心护理管理质量的提升。     

靛玉红甲肟对奈达铂抗人食管癌EC-1细胞的化疗增效作用

 目的 探讨靛玉红甲肟(Indirubin-3′-monoxime,IRO)与奈达铂联合应用对人食管癌细胞株EC-1的影响及其作用机制。方法 经不同浓度的IRO和(或)奈达铂处理EC-1细胞后,用MTT法测定细胞增殖抑制效应;流式细胞术观察细胞周期变化;免疫组织化学法观察凋亡相关基因表达变化。结果与IRO或奈达铂单药作用相比,IRO与奈达铂联合应用可显著增强人食管癌细胞增殖抑制作用,联合用药后细胞周期分布发生改变,G₀/G₁期细胞比例下降,G₂/M期细胞比例上升,癌细胞Bcl-2基因表达下降,Bax表达增强。结论 IRO与奈达铂联合应用具有协同抗食管癌作用,其机制可能与细胞周期调控及凋亡相关基因表达调控有关。     

CDK1 and Cyclin A Expression is Linked to Cell Proliferation and Associated with Prognosis in Non-Hodgkin's Lymphomas

Cellular proliferation is regulated by several kinasic complexes associating cyclins and their catalytic subunits cyclin-dependent kinases (CDKs). In order to gain insight into the mechanisms underlying proliferation in non-Hodgkin's lymphoma (NHL), we examined the expression of certain cell cycle regulatory proteins normally expressed in lymphoid cells, cyclins A, B, D3 and E and cdkl, 2, 4, and 6. In 70 patients presenting a previously untreated lymphoma, cyclins and CDKs were studied by Western blotting and quantified by densitomerry. Flow cytometry study of DNA content was canied out for all patients in order to study cell proliferation and level of ploidy. The results were analysed according to the histological types, the immunological phenotypes of the lymphomas and the outcome of the patients.

Cdk1 and cyclin A were correlated with the percentage of cells in S and S+G2/M phases, and significantly different according to the grade of malignancy, with the lowest expression in low-, and the highest in high-grade NHL according to the Working Formulation. In B-NHLs, cdkl, cyclin A, as well as cdk2, cyclin D3 and E expression was higher in the aneuploid than in the euploid group. Our results point to some particularities of cell cycle regulation in two lymphoma sub-types: I) a low expression of cyclin D3 and cdk6 in mantle cell lymphomas and 2) a discrepancy between the high proliferative activity and the level of protein expression in Burkitt's lymphomas. CDKl and cyclin A showed a significant prognostic value for achievement of complete remission (Cdk 1) and for both disease free (cyclin A) and overall survival (cyclin A and cdkl): low protein level was associated with the best prognosis in B-NHLs.

Our results show that differential cell cycle regulating protein expression may be associated with different biological and clinical behaviour of NHLs and confirm the usefulness of the study of cell cycle regulation as a tool for understanding lymphoid malignancies.     

蓝萼香茶菜乙素对Rb细胞增殖和细胞周期的影响

目的探讨蓝萼香茶菜乙素对体外培养的人视网膜母细胞瘤(retinoblastoma,Rb)细胞的增殖及细胞周期的影响。方法 MTT法测定不同浓度(2.5μmol.L-1、5.0μmol.L-1、10.0μmol.L-1、20.0μmol.L-1和40.0μmol.L-1)的蓝萼香茶菜乙素作用24h及48h后对人Rb细胞株的影响;碘化丙啶染色、流式细胞术观察蓝萼香茶菜乙素对Rb细胞周期及细胞凋亡的影响。结果蓝萼香茶菜乙素不同剂量组对Rb细胞均有显著细胞毒作用。蓝萼香茶菜乙素处理24h和48h后对Rb细胞株的IC50分别为79.15μmol.L-1和10.88μmol.L-1,剂量-效应关系较为明显;对照组和不同药物浓度组(2.5μmol.L-1组、5.0μmol.L-1组、10.0μmol.L-1组、20.0μmol.L-1组、40.0μmol.L-1组)作用Rb细胞24h后,细胞凋亡率分别为:(2.01±0.03)%、(6.21±0.91)%、(10.21±2.06)%、(16.83±2.61)%、(25.17±2.11)%和(35.29±1.04)%;作用48h后,对应细胞凋亡率分别为:(2.41±0.73)%、(7.94±3.01)%、(12.08±2.43)%、(20.11±2.37)%、(35.18±3.48)%和(51.20±2.93)%。这一结果也显示:随着药物浓度增加,Rb细胞凋亡率也随之升高。结论蓝萼香茶菜乙素通过诱导Rb细胞株凋亡发挥抑制人Rb增殖的作用。     

神经肽Y对成年大鼠睡眠的影响

目的 :探讨神经肽Y(NPY)与睡眠 -觉醒周期的关系。方法 :4 4只成年雌性SD大鼠 ,随机分为 6组 ,分别为侧脑室微量 (5 μl)注射NPY(1g/L)、α - 1受体阻断剂育亨宾 (30 μmol/L) ,育亨宾 +NPY ,α - 2受体阻断剂哌唑嗪 (30 μmol/L) ,哌唑嗪 +NPY及生理盐水 (对照组 )。采用多导描记技术观察大鼠在清醒状态下 ,统计给药后各组大鼠 6h的慢波睡眠 (SWS)、快眼动睡眠 (REMS)及总睡眠 (TS)时间。结果 :单独注射NPY可以促进大鼠的SWS ;先给予育亨宾再给予NPY ,大鼠SWS、REMS及TS时间较生理盐水对照组显著降低 (P <0 .0 1 )。单独给予育亨宾、哌唑嗪或哌唑嗪 +NPY ,对大鼠睡眠无明显影响。结论 :NPY具有一定的促眠作用 ,且与α- 2受体有关     

Skp2在三氧化二砷诱导的肝癌SMMC-7721细胞周期阻滞中的表达变化和意义

目的: 探讨三氧化二砷(As₂O₃)对肝癌SMMC-7721细胞周期的影响,及其与细胞周期素依赖性激酶抑制因子(CDKI)p27^kip1和p27^kip1相关蛋白S期激酶相关蛋白2(S-phase kinase-associated protein2,Skp2)的关系,为临床应用提供依据。 方法: 体外培养人肝癌细胞株SMMC-7721,用2μmol/LAs₂O₃处理72h,流式细胞仪检测细胞周期变化,采用核浆分离、Western Blot技术及细胞免疫荧光技术检测该过程中p27^kip1、Skp2在SMMC-7721细胞中的表达变化及亚细胞定位情况。 结果: 与对照组比较,As₂O₃使SMMC-7721细胞周期阻滞在G₂/M期。经As₂O₃作用的人肝癌细胞p27^kip1蛋白水平增加,Skp2蛋白水平降低,同时27^kip1发生从胞浆到胞核的易位。 结论: As₂O₃可下调Skp2的表达,从而促进SMMC-7721细胞中p27^kip1的积聚,干扰细胞周期的进程,抑制SMMC-7721细胞的增殖。     

红参含药血清对人乳腺癌MCF?鄄7细胞增殖和细胞周期的影响

[目的]研究红参含药血清对人乳腺癌MCF-7细胞增殖和细胞周期的影响.[方法]采用MTT法观察红参含药血清对人乳腺癌细胞MCF-7的增殖活性.流式细胞术检测人乳腺癌细胞MCF-7的细胞周期、细胞凋亡率.[结果]红参低剂量含药血清对MCF-7细胞有促增殖作用,24、48、72h时增殖率分别为105.9％、115.9％和121.5％.而高剂量对MCF-7细胞表现为抑制作用.红参低剂量组使MCF-7细胞S期DNA含量比例明显增多,高剂量组使细胞周期阻滞在G0/G1期,并能够诱导细胞凋亡,凋亡率达26.86％.[结论]红参含药血清在高剂量时抑制MCF-7细胞生长,低剂量时对细胞具有促增殖作用.