阿尔泰锦鸡儿不同药用部位主要黄酮苷的含量测定

目的 测定采集于新疆阿勒泰地区的10批次阿尔泰锦鸡儿根、茎、叶、花与果实药材中芦丁和山奈酚-3-O-芸香糖苷的含量。方法 色谱条件：Inertsil ODS-3色谱柱（250 mm×4.6 mm,5 μm）,检测波长254 nm,流速1.0 mL·min^-1,进样量5 μL,柱温25℃,流动相为0.2%磷酸（A）-乙腈（B）,线性梯度洗脱（0~5 min,10%→14% B;5~10 min,14%→17% B;10~16 min,17%→20% B;16~21 min,20%→22% B;21~30 min,22%→25% B;30~40 min,25% B）。结果 10批次阿尔泰锦鸡儿叶、花与果实药材中芦丁的平均含量为：果实6.83 mg·g^-1,叶6.16 mg·g^-1,花3.58 mg·g^-1;山奈酚-3-O-芸香糖苷的平均含量为：果实1.48 mg·g^-1,叶2.44 mg·g^-1,花1.01 mg·g^-1。根与茎供试品溶液中均未检测到这2种黄酮苷成分。结论 建立的方法简便、稳定性、重复性良好,为阿尔泰锦鸡儿药材黄酮类成分的研究提供了实验基础,亦为建立该药材的质量标准提供了重要的实验数据。     

天胡荽有效部位HS抗大鼠免疫型肝纤维化的实验研究

目的：研究天胡荽有效部位HS抗肝纤维化的作用。方法：复制猪血清所致的大鼠肝纤维化模型,设立空白对照组,模型对照组,γ干扰素组,HS高剂量组（1.5g/kg）与低剂量组（0.5g/kg）,观察HS对丙氨酸氨基转移酶（ALT）,门冬氨酸氨基转移酶（AST）,白蛋白（Alb）,总蛋白,羟脯氨酸（Hyp）的含量以及对肝脏病理形态学的影响。结果：HS显著降低大鼠血清中的ALT,AST水平以及肝组织中的Hyp水平;组织病理学结果显示：HS高,低剂量组大鼠肝组织纤维化程度都有一定改善。结论：HS具有良好的抗免疫型肝纤维化作用。     

飞扬草混淆品通乳草的鉴别

本文对飞扬草混淆品通乳草进行了性状和显微鉴别,并与飞扬草进行了比较,为飞扬草的鉴别提供依据。     

金雀根中两种有效成分的分离鉴定及其生理活性测定

目的 从豆科植物锦鸡儿Caragana sinica（Buc＇hoz）Rehd的根（中药金雀根）中分离有效成分并研究其生理活性.方法 用柱层析和薄层层析将有效成分从乙醇浸膏中分离,光谱分析鉴定其结构.用MTT法测定化合物Ⅰ的生理活性.结果 分离得到两种有效成分,分别鉴定为kobophenol A（Ⅰ）和（-）-ampelopsin F（Ⅱ）,化合物Ⅰ具有刺激成骨细胞增殖的生理活性.结论 kobophenol A（Ⅰ）和（-）-ampelopsin F（Ⅱ）是金雀根中的两种有效成分,前者具有刺激成骨细胞增殖的活性.     

皂角提取物对心肌缺血犬心肌梗死的保护作用

目的观察皂角提取物对犬心肌梗死的保护作用,并评价其对犬心肌缺血的药效。方法将20只实验犬随机分为皂角提取物组、地奥心血康组、银杏叶组和模型组;手术结扎犬冠状动脉左前降支(LAD)造成急性心肌梗死模型,观察各组犬血流动力学和心肌氧代谢参数及心肌梗死面积、血清酶学变化。结果皂角提取物可显著地降低心肌缺血犬的心肌耗氧量,缩小心肌梗死范围,降低血清磷酸肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性。结论皂角提取物能显著减少心肌耗氧量,对缺血心肌具有保护作用。     

金雀根二苯乙烯低聚体成分的研究

从豆科植物锦鸡儿Caragana sinica(Buc‘hoz) Rehd的根（中药金雀根）中分离出4个二苯乙烯低聚体。根据理化性质和光谱分析,分别鉴定为（＋）－α－viniferin(1)、miyabenol C(2)、pallidol(3)、kobophenol A(4)。其中化合物3为首次从该植物中分得。     

The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves

Moringa oleifera is used in Thai traditional medicine as cardiotonic. Recent studies demonstrated its hypocholesterolaemic effect. However, to be clinically useful, more scientific data are needed. AIM OF THE STUDY: We investigated the antioxidant, hypolipidaemic and antiatherosclerotic activities of Moringa oleifera leaf extract. MATERIALS AND METHODS: Scavenging activity of the extract on 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH), and the inhibitory effect on Cu(2+)-induced low-density lipoprotein (LDL) oxidation were determined in in vitro experiment. The effects of the extract on cholesterol levels, conjugated diene (CD) and thiobarbituric acid reactive substances (TBARS) and plaque formations in cholesterol-fed rabbits were investigated. RESULTS: We found that in scavenging DPPH radicals the extract and Trolox had IC(50) of 78.15+/-0.92 and 2.14+/-0.12microg/ml, respectively. The extract significantly (P<0.05) prolonged the lag-time of CD formation and inhibited TBARS formation in both in vitro and ex vivo experiments in a dose-dependent manner. In hypercholesterol-fed rabbits, at 12 weeks of treatment, it significantly (P<0.05) lowered the cholesterol levels and reduced the atherosclerotic plaque formation to about 50 and 86%, respectively. These effects were at degrees comparable to those of simvastatin. CONCLUSIONS: The results indicate that this plant possesses antioxidant, hypolipidaemic and antiatherosclerotic activities and has therapeutic potential for the prevention of cardiovascular diseases.     

Inhibition effect of Caragana sinica root extracts on Osteoarthritis through MAPKs,NF-κB signaling pathway

Osteoarthritis (OA) is a common joint disease characterized by degradation and inflammation of cartilage extracellular matrix. We aimed to evaluate the protective effect of Caragana sinica root (CSR) on interleukin (IL)-1β-stimulated rat chondrocytes and a monosodium iodoacetate (MIA)-induced model of OA. In vitro, cell viability of CSR-treated chondrocytes was measured by MTT assay. The mRNA expression of Matrix metallopeptidases (MMPs), a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs) and extracellular matrix (ECM) were analyzed by quantitative real-time PCR (qRT-PCR). Moreover, the protein expression of MAPK (phosphorylation of EKR, JNK, p38), inhibitory kappa B (IκBα) and nuclear factor-kappa B (NF-κB p65) was detected by western blot analysis. In vivo, the production of nitric oxide (NO) was detected by Griess reagent, while those of inflammatory mediators, MMPs and ECM were detected by ELISA. The degree of OA was evaluated by histopathological analyses, Osteoarthritis Research Society International (OARSI) score and micro-CT analysis. CSR significantly inhibited the expression of MMPs, ADAMTSs and the degradation of ECM in IL-1β-stimulated chondrocytes. Furthermore, CSR significantly suppressed IL-1β-stimulated of MAPKs, NF-κB signaling pathway. In vivo, CSR and Indomethacin inhibited the production of inflammatory mediators, MMPs and degradation of ECM in MIA-induced model of OA. In addition, CSR improved the severity of OA. Taken together, these results suggest CSR is a potential therapeutic active agent in the treatment of OA.     

基于聚类分析和主成分分析的不同产地龙船花全草质量评价＊

目的 分析测定和对比不同产地龙船花全草中京尼平苷酸、绿原酸、东莨菪内酯、槲皮素和山奈酚5个成分含量，建立快速测定其含量的方法。方法 采用高效液相色谱法（High performance liquid chromatography，HPLC）测定5个成分含量，色谱柱为Diamonsil-C₁₈柱（4.6 mm × 250 mm，5 μm），流动相为0.05%甲酸水溶液（A）-乙腈（B），梯度洗脱，流速1 mL·min^-1，柱温25℃，检测波长为268 nm（0-12 min）、344 nm（12-37 min）、350 nm（37-41 min）、254 nm（41-50 min）；并以检测出的含量为指标，使用SPSS 20.0软件对18批样品进行聚类分析和主成分分析。结果 京尼平苷酸、绿原酸、东莨菪内酯、槲皮素和山奈酚色谱峰分离良好；5种成分在各自范围内线性关系良好（r ≥ 0.9991），平均加样回收率93.76%-99.91%，RSD为0.66%-1.47%。聚类分析结果显示18批样品可分为3大类：S7-S12聚为一类，S1-S6聚为一类，S13-S18聚为一类。经主成分分析，主成分1、主成分2、主成分3是影响药材样品质量评价的主要因子，3个主成分的累积方差贡献率为93.916%，以S14的主成分综合得分最高。结论 所建立的龙船花全草HPLC含量测定方法可用于龙船花的质量控制，聚类分析方法和主成分分析法也可为龙船花种植、栽培和质量评价提供依据。     

芳香新塔花化学成分的分离与鉴定

目的为进一步开发利用芳香新塔花(Ziziphora clinopodioides Lam.)植物资源提供依据。方法采用反复硅胶柱色谱、葡聚糖凝胶LH-20柱色谱、聚酰胺柱色谱等方法分离纯化,并通过理化常数测定与波谱分析鉴定化学结构。结果从芳香新塔花的乙醇提取物中分离鉴定了7个化合物,分别为槲皮素(quercetin,1),槲皮素3-O-β-D-吡喃葡萄糖苷(quercetin3-O-β-D-glucopyranoside,2)、迷迭香酸甲酯(methyl rosmarinate,3),迷迭香酸(rosmarinic acid,4),3-(3′,4′-二羟基苯基)-乳酸甲酯(3-(3′,4′-dihydroxyphenyl)methyl lactate,5),原儿茶酸(protocatechuic acid,6),半乳糖醇(galactitol,7)。结论化合物2~7为首次从新塔花属植物中分离得到。