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41.
Danuta Wasserman Christer HellstrÖm Jerzy Wasserman Olof Beck Eva Andersson Marie Åsberg 《Archives of Suicide Research》1997,3(3):153-169
The aim of this study was to investigate markers of serotonin and immune function in suicidal patients. Cytotoxic activity of natural killer cells (NK) and CD16 lymphocytes were studied in 28 suicide attempters and 26 healthy controls, and related in patients to 5-hydroxyindoleacetic acid (5-HIAA) in cerebrospinal fluid (CSF). Patients with CSF 5-HIAA below the median had significantly lower NK cell activity than other patients. CD16 cell frequency was significantly lower in patients than in controls, and patients also tended to have lower NK cell cytotoxicity than healthy controls. There were no statistically significant correlations between 4-hydroxy-3methoxyphenyl glycol (HMPG), homovanillic acid (HVA), CSF cortisol and NK cell activity. The results support the hypothesis of compromised immune function in suicidal patients with evidence of disordered serotonin function. 相似文献
42.
43.
30例新鲜胃癌标本,采用多聚酶链反应技术检测人类乳头瘤病毒16、18感染、并采用多聚酶链反应-单链构象多态性技术对p53基因突变进行检测。结果:HPV15阳性率为40.0%,未发现HPV18感染:P53基因突变率为50.0%,HPV16感染和P53基因突变同时存在者为16.7%。 相似文献
44.
血清CA125检测对子宫内膜异位症的临床诊断价值 总被引:2,自引:0,他引:2
巫云 《中国交通医学杂志》2003,17(5):535-536
目的 :探讨血清CA12 5测定对术前子宫内膜异位症的诊断价值。方法 :99例子宫内膜异位症经腹手术前测定CA12 5水平 ,并与 90例作对照比较。结果 :子宫内膜异位症CA12 5阳性率 >3 5U /ml,为 5 7.5 7% ,对照组为 8.88% (P <0 .0 1)。内膜异位囊肿高于卵巢囊肿 (P <0 .0 1) ,子宫腺肌病高于子宫肌瘤 (P <0 .0 5 )。结论 :血清CA12 5测定对子宫内膜异位症有较理想的诊断价值 ,对异位囊肿与卵巢囊肿 ,子宫腺肌病与子宫肌瘤有鉴别意义 相似文献
45.
幕上星形细胞肿瘤MRI表现与VEGF、P16、Ki-67相关性研究 总被引:1,自引:0,他引:1
探讨星形细胞肿瘤MRI表现与P16、Ki—67、血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的相关性。材料和方法:收集我院术前经MRI诊断并经手术病理证实的幕上星形细胞肿瘤53例,行常规平扫描后静脉注射Gd—DTPA增强成像;同时采用HE染色及链菌素生物素—过氧化酶连接法进行VEGF、Ki—67、P16的免疫组织化学染色,测定它们的表达指数。结果:星形细胞肿瘤的信号不均匀性、坏死、水肿、占位效应、强化程度与VEGF、Ki—67、P16的表达相关,且随着星形细胞肿瘤恶性程度的增高VEGF、Ki—67的表达增加;P16的表达率降低。结论:星形细胞肿瘤的MRI表现与VEGF、Ki—67、P16的表达密切相关,MRI可以间接判断星形细胞肿瘤的某些生物学行为。 相似文献
46.
82例高血压患者16项人格特质测验 总被引:1,自引:0,他引:1
研究目的应用卡特尔16项人格特质测验探讨原发性高血压患者的人格特征。研究背景高血压病是危害人类健康的常见病之一,发病率逐年上升,但其病因至今尚未阐明,故有必要从心理、人格方面来探讨高血压的发病原因,以利于高血压病的防治。研究方法本文对82例均由2名以上内科医师确诊为原发性高血压、且现在高压仍保持在20/12.7kPa以上的患者进行卡特尔16项人格特质测验,并与78例人群对照分析。结果高血压患者在C-稳定性、E-峙强性、Q-紧张性以及在双重人格因素类型中的适应与焦虑方面与正常对照组有显著差异(P<0.05)。结论心理-社会因素是促使高血压发生的重要因素,它对高血压病因的阐明有重要意义,同时也使我们越来越重视运用心理、行为疗法,如生物反馈、各种松驰疗法等去防治高血压。 相似文献
47.
Idziorek Khalife Billaut-Mulot Hermann Aumercier Mouton Capron Bahr 《Clinical and experimental immunology》1998,112(1):84-91
The chemoattractant cytokine IL-16 has been reported to suppress lymphocyte activation and to inhibit HIV-1 replication in acutely infected T cells. We have cloned and expressed human IL-16 in Escherichia coli and investigated whether the recombinant protein could regulate the level of lymphocyte apoptosis from HIV-1-infected subjects. After purification and refolding, only 2–10% of the recombinant cytokine was present in a biologically active homotetrameric form. This could explain the need for high concentrations of the bacterially derived IL-16 to induce significant inhibition of HIV-1 replication. Addition of IL-16 to unstimulated peripheral blood mononuclear cell (PBMC) cultures from HIV-1-infected subjects did not modify the observed level of spontaneous lymphocyte apoptosis. In contrast, IL-16 added to PBMC cultures stimulated with anti-CD3, anti-CD95 or dexamethasone reduced significantly the percentage of lymphocytes undergoing AICD. This effect was found to correlate with the ability of the cytokine to decrease CD95 expression on activated CD4+ T cells. Comparative studies on PBMC from healthy individuals indicated that the regulation of apoptosis levels by IL-16 is a complex phenomenon and could depend on the nature of the activator used and/or the immune status of lymphocytes tested. The outcome of CD4 cross-linking on T cells by various ligands is discussed in the context of the observed beneficial activities of IL-16 and its potential role in the treatment of HIV disease. 相似文献
48.
用PCR—RFLP和16SrDNA指纹图法分析幽门螺杆菌基因型 总被引:1,自引:1,他引:0
本文建立了PCR-RFLP和16srDNA指纹图法.对19株幽门螺杆菌(HP)进行基因型分析:HP尿素酶C基因的PCR扩增产物.分别用HindⅢ、HaeⅢ、AluⅠ酶切,结果显示:每个酶均将19株HP分为3种RFLP图谱.综合HindⅢ,HaeⅢ和AluⅠ酶切结果,19株HP分为10个酶切带型;PCR扩增HP标准株16SrRNA基因,地高辛标记制备550bp探针,19株HPDNA分别经HaeⅢ和EcoRⅠ酶切、电泳后,通过Southern杂交获16SrDNA指纹图,结果显示:HaeⅢ酶切分为14个杂交带型,EcoRⅠ酶切19株HP杂交带型均不同。本实验表明:上述两种方法重复性好,分群力高,可准确有效地对HP作出鉴定并将其分型。19株HP株间存在基因型差异。 相似文献
49.
Association analysis of CA repeat polymorphism of the endothelial nitric oxide synthase gene with essential hypertension in Japanese 总被引:7,自引:0,他引:7
T. Nakayama M. Soma Y. Takahashi Y. Izumi K. Kanmatsuse M. Esumi 《Clinical genetics》1997,51(1):26-30
The nitric oxide synthase (NOS) gene is thought to be associated with essential hypertension (EH), because NO is implicated in endothelium-mediated vasodilation. We investigated the possible association between the alleles of simple tandem repeat DNA polymorphism of the endothelial constitutive NOS (cNOS) gene and EH in Japanese subjects. In all, 100 patients with EH and 123 subjects with normal blood pressure were studied. Polymerase chain reaction was used to amplify the CA repeat site in the endothelial cNOS gene and alleles based on the CA repeat number were determined. The allele frequencies in the hypertensive group and normotensive group were then compared. Twenty-three alleles were identified in this study of Japanese subjects. The overall distributions of allele frequencies in the two groups were not significantly different. However, comparing the allele frequencies in the EH group without left ventricular hypertrophy (LVH) and the normotensive group, the overall distributions were significantly different (p = 0.019). The 33-repeat allele was found more frequently in the EH group without LVH than in the normotensive group (p = 0.000047, Odds ratio = 3.71). In conclusion, the 33-repeat allele of the endothelial cNOS gene is associated with EH without LVH, and may be a genetic marker of EH in Japanese subjects. 相似文献
50.
Helene Z. Hill Maimu Ohanian George J. Hill Richard Winne 《Journal of surgical oncology》1987,34(4):264-267
Intraoperative radiation therapy (IORT) research is limited by the lack of small-animal models. We have implanted B16 melanoma into mouse kidneys, which we subsequently operated upon and irradiated with beta rays from a 90Sr ophthalmic applicator. The IORT has effectively prolonged survival and produced some cures. The strategy should be applicable to other murine tumors and to other internal implantation sites. 相似文献