胃肠道肿瘤病人围手术期细胞因子及蛋白质代谢的变化

目的 :探讨胃肠道肿瘤病人手术后肿瘤坏死因子 α (TNF α)、白介素 6 (IL 6 )、白介素 10 (IL 10 )的变化以及与蛋白质代谢的关系。　方法 :用酶联免疫法 (ELISA)检测 19例胃肠道肿瘤根治术病人术前和术后 1、3、5天血清TNF α、IL 6和IL 10水平 ,同时检测蛋白质分解代谢相关指标、2 4h尿中尿素和肌酐排泄量。　结果 :胃肠道肿瘤根治术后病人TNF α呈下降趋势 ,术后第 1天较术前明显下降 (P <0 .0 5 )。IL 6和IL 10术后升高 (P <0 .0 1) ,尤以术后第 1天升高明显。术后 2 4h尿中尿素和肌酐排泄量明显升高。　结论 :胃肠道肿瘤根治术后病人IL 6和IL 10的升高支持手术应激导致的细胞因子释放变化 ,但TNF α呈下降趋势 ,可能与胃肠道肿瘤病人术前TNF α的高表达有关。术后蛋白质分解代谢增加 ,可能与细胞因子IL 6和IL 10的升高有关。     

Glucocorticoid regulation of eicosanoid production by glial cells under basal and stimulated conditions

We measured the production of two eicosanoids, prostaglandin E₂ and thromboxane-B₂, by rat glial cell cultures under basal conditions, following stimulation with phorbol-12-myristate-13-acetate and the bacterial endotoxin lipopolysaccharide, and following treatment with synthetic glucocorticoids. Stimulation of rat glial cells in culture with either phorbol-12-myristate-13-acetate or lipopolysaccharide caused a 1.5–5.0-fold increase in prostaglandin E₂ production, but did not affect thromboxane production. Pretreatment of the cultures with dexamethasone markedly inhibited the stimulated production of prostaglandin E₂ but had only a modest effect on basal production. Dexamethasone did not affect the activity of the enzyme protein kinase C, a putative regulator of eicosanoid synthesis. Our findings show that glucocorticoids have the potential to modulate central nervous system eicosanoid production particularly under conditions of stimulated production, such as inflammatory and demyelinating disorders. This mechanism may explain, at least in part, the therapeutic benefit of glucocorticoids in patients with multiple sclerosis.     

Fentanyl and the interleukin-6 response to surgery

It has been suggested that large doses of opioids may suppress the interleukin-6 response to surgery. We examined the effects of the supplementation of inhalational anaesthesia with either 3 or 15 μg.kg⁻¹ fentanyl on the circulating interleukin-6, interleukin-8, C-reactive protein, cortisol and glucose concentrations in 16 patients undergoing pelvic surgery. In both groups, surgery evoked the expected glucose, cortisol and interleukin-6 response but no increase in interleukin-8 was detected. There were no significant differences between the two groups. We conclude that the supplementation of inhalational anaesthesia with conventional doses of opioids does not modify the cytokine response to surgery.     

油菜和向日葵花粉的成分分析及其蛋白质营养评价

<正> 花粉在古代就曾作为强身保健食品。美、英两国的科学家曾分析了花粉的成分,发现花粉由96种元素组成,其中有维生素、矿物质、酶、蛋白质和某些其它物质。美国明尼苏达大学曾做过花粉对动物的增重实验。当41～150日龄小猪饲料中添加2～3％的玉米花粉时,体重增长明显快于对照组,     

乳腺癌组织P21WAF1基因多态性与蛋白表达的关系

目的 :探讨乳腺癌组织p2 1WAF1DNA多态性与蛋白表达的关系。方法 :分别采用聚合酶链反应单链构象多态性技术 (PCR -SSCP)和免疫组化S -P法检测 10 0例乳腺癌石蜡包埋组织和 4 0例乳腺良性病变组织p2 1WAF1DNA多态性和蛋白表达。结果 :18% (18/ 10 0 )的乳腺癌组织和 5 % (2 / 4 0 )对照组组织分别出现两种p2 1WAF1DNA多态性 ,前者明显高于后者 ,组间有显著差异 (χ2 =3 94 ,P <0 0 5 ) ;5 0 % (5 0 / 10 0 )的乳腺癌组织和 12 5 % (5 / 4 0 )对照组组织表达p2 1WAF1蛋白 ,前者明显高于后者 ,组间有显著差异 (χ2 =16 84 ,P <0 0 1) ;10 0 % (18/ 18例 )的具有p2 1WAF1DNA多态性的乳腺癌组织p2 1WAF1蛋白表达阳性 ,39% (32 / 82 )的无p2 1WAF1DNA多态性乳腺癌组织蛋白表达阳性 ,p2 1WAF1DNA多态性组明显高于无多态性组 ,组间有显著差异 (χ2 =2 1 95 ,P <0 0 1) ;p2 1WAF1DNA多态性与p2 1WAF1蛋白表达呈正相关 (r =0 5 76 ,P <0 0 1)。结论 :乳腺癌组织p2 1WAF1DNA多态性可能产生不同的转录本并生成相应的蛋白质分子。     

Wnt-frizzled信号通路与心血管疾病关系的研究进展

Many researches have focused on the wnt- frizzled cascade in the recent years, while much work has been done in neoplastic diseases and embryology, the role of the wnt- frizzled signal transduction pathway in cardiovascular diseases has only recently begun to be explored. It plays a very important role in many physiological and pathophysiological processes, such as its transduction pathway, the healing after myocardial infarction, the proliferation, differentiation and orientation of cardiomyocytes, angiogenesis/neovascularization, cardiac hypertrophy and heart failure, the deposition of the extracellular matrix and so on. This article is aimed at its relation with myocardial infarction and the role of this pathway in cardiovascular diseases.     

三氧化二砷对人脐静脉内皮细胞中蛋白激酶C活性和白细胞介素-15分泌、表达的影响及其临床意义

目的 通过研究三氧化二砷(As2O3)对人脐静脉内皮细胞(HUVEC)中蛋白激酶C(PKC)活性和白细胞介素(IL)-15表达和分泌的影响,探讨内皮细胞在As2O3抑制白血病或肿瘤中的作用。方法用不同浓度的As2O3作用于培养的HUVEC细胞,测定其生长情况、胞膜和胞浆PKC活性以及对IL-15基因表达和分泌的影响。结果0.25 μmol／L~50 μmol／L的.As2O3均抑制HUVEC细胞的增殖(P<0.01),随着药物浓度或作用时间的增加,细胞逐渐死亡。在As2O3作用细胞4 h测定胞浆和胞膜的PKC活性,两者在不同浓度作用下活性水平均得到提升(P<0.01),但胞膜的活性上升幅度更大。在As2O3的测定浓度范围内,PKC的活性水平与As2O3的浓度呈正相关。1、5、16 μmol／L的As2O3导致IL-15基因表达和分泌水平的增加(P<0.01),并呈浓度依赖性关系。结论As2O3可能是通过激活内皮细胞的PKC活性,促进IL-15或其他细胞因子的分泌,来抑制白血病或肿瘤发展的。     

Total and free tissue factor pathway inhibitor in pregnancy hypertension.

OBJECTIVE: To clarify the role played by tissue factor pathway inhibitor (TFPI) in pregnancy hypertension. METHODS: Using enzyme-linked immunosorbent assays, hemostatic measurements were obtained for women with pre-eclampsia (n=51), nonproteinuric hypertension of pregnancy (n=62), postpartum pre-eclampsia 24 h after childbirth (n=31), and no hypertension (healthy pregnant controls, n=100). RESULTS: There was a significant increase in circulating free TFPI levels in women with pre-eclampsia (9.7+/-6.2 ng/mL) or nonproteinuric hypertension of pregnancy (8.3+/-5.3 ng/mL) compared with healthy controls (5.3+/-2.1 ng/mL). In women with pre-eclampsia the levels remained elevated after placental delivery (10.6+/-4.0 ng/mL). Free protein S levels were significantly higher in women with pre-eclampsia (40.0%+/-10.7%), nonproteinuric hypertension of pregnancy (37.1%+/-12.5%), or postpartum pre-eclampsia (39.3%+/-9.1%) than in healthy pregnant controls (32.2%+/-8.5%). CONCLUSION: Increased levels of the physiologically active free forms of TFPI and free protein S, 2 coagulation inhibitors, may protect women with pregnancy-induced hypertension from the risks of hemostatic activation.     

地塞米松对内毒素作用下人脐静脉内皮细胞表达组织因子、凝血酶调节蛋白和蛋白S的影响

目的观察地塞米松(DEX)对内毒素(LPS)作用于人脐静脉内皮细胞(HUVEC)后表达组织因子(TF)、凝血酶调节蛋白(TM)和蛋白S(PS)的影响.方法24孔板培养的第1～5代HUVEC,在含不同浓度LPS和DEX的无血清培养基中培养一定时间后裂解细胞,应用酶联免疫吸附法(ELISA)测定裂解液中的TF、TM和PS含量.结果在LPS刺激下,HUVEC表达TF的量呈剂量依赖性升高,在LPS0.1μg/ml下TF的表达量为对照组的4倍(P＜0.01),同时加入0.5 μg/ml和1.0 μg/ml DEX则可使TF的表达量由128.3±25.7 pg/105细胞分别降至94.9±19.4 pg/105细胞和98.8±7.8 pg/105细胞(P＜0.05);LPS(0.01～10μg/ml)可抑制HUVEC表达TM,在LPS 10 μg/ml下,TM表达量降至对照组的60%(P＜0.05).DEX可拮抗LPS抑制HUVEC表达TM的效应,0.1 μg/ml、0.5 μg/ml和1.0 μg/ml DEX可使LPS(10 μg/ml)作用下TM的表达量由0.282±0.014 ng/105细胞分别增至0.409±0.009、0.462±0.017和0.362±0.019 ng/105细胞(P＜0.05);LPS(0～10 μg/ml)可抑制HUVEC表达PS,在LPS浓度1.0 μg/ml及10 μg/ml时,PS的表达量分别为对照组的43%和38%(P＜0.01).DEX则拮抗LPS抑制HUVEC表达PS的效应,0.5 μg/ml DEX可使LPS刺激下PS的表达量由13.1±4.8%/2×105细胞增至48.5±10.2%/2×105细胞(P＜0.01).结论LPS促进HUVEC表达TF,抑制HUVEC表达TM和PS.DEX能部分拮抗上述作用,提示DEX可能纠正内毒素血症时的高凝状态.     

Protein carbonyls are acutely elevated following single set anaerobic exercise in resistance trained men

The purpose of this investigation was to determine if a single set of strenuous squat exercise would result in an acute oxidative stress, as demonstrated previously by a single sprint. Thirteen resistance trained men performed one set of 15 repetitions of barbell squats using 70% of one repetition maximum and a 30 s maximal cycle sprint on two different occasions. The total work performed was calculated for each exercise bout. Heart rate, perceived exertion, blood lactate, protein carbonyls, 8-hydroxydeoxyguanosine, and malondialdehyde were measured before and within 1 min following exercise. No differences were noted between the squat and sprint tests for total work, heart rate or perceived exertion. An exercise test by time interaction was evident for blood lactate with values greater following sprinting compared to squatting (P = 0.0005). Postexercise protein carbonyls were not different between exercise tests but were elevated above rest (P = 0.04) by 111% and 74% following sprinting and squatting, respectively, while 8-hydroxydeoxyguanosine and malondialdehyde were relatively unaffected by either exercise test. These data indicate that a single bout of strenuous squatting and sprinting performed by resistance trained men results in elevated protein carbonyls, while having little impact on 8-hydroxydeoxyguanosine or malondialdehyde during the immediate postexercise period.