垂体腺瘤PTTG、bFGF mRNA表达与侵袭性的关系

目的：检测垂体腺瘤组织中垂体肿瘤转化基因(PTTG）、bFGF mRNA的表达，分析其与垂体腺瘤侵袭性的关系及临床意义。方法：应用逆转录PCR(RT—PCR)方法检测30例垂体腺瘤组织标本中PTTG、bFGF mRNA的表达，分析二者与垂体腺瘤侵袭性的关系。结果：侵袭性垂体腺瘤组的PTTG、mRNA表达、bFGF mRNA表达均较非侵袭性腺瘤组增高，其差异具有统计学意义。结论：垂体腺瘤侵袭性的发生与垂体肿瘤转化基因(PTTG)表达增高及其上调血管生成正调节因子bFGF并表达、促进血管生成有关，     

浅谈针刺手法的基本功锻炼

针刺手法的运用是针灸临床治疗的一个关键环节。针刺手法的基本功锻炼是提高针刺手法操作技术的重要途径。本文详细介绍了针刺手法基本功锻炼的步骤和方法。     

Clotrimazole, an Imidazole Antimycotic, Is a Potent Inhibitor of Angiogenesis

Clotrimazole, an imidazole antimycotic, interferes with the rise in cytosolic Ca²⁺ and inhibits cell proliferation in a reversible manner. Here we describe the effect of clotrimazole on vascular endothelial cells (ECs). Clotrimazole inhibited the proliferation of ECs stimulated with typical angiogenic growth factors; vascular endothelial growth factor and basic fibroblast growth factor (bFGF). This inhibitory effect of clotrimazole was dose-dependent and the maximal inhibition was observed at a concentration of 10 m M . We did not observe any increase in ⁵¹Cr release from ECs during treatment with 10 μ M . clotrimazole. Moreover, clotrimazole inhibited the basal and bFGF-stimulated migration of ECs. As clotrimazole inhibited two principle components of angiogenesis; the proliferation and migration of ECs, we examined whether clotrimazole inhibited angiogenesis. Tube formation by ECs in type 1 collagen gel was investigated, and clotrimazole was found to be significantly inhibitory. The inhibitory effect of clotrimazole on angiogenesis was further confirmed in an in vivo angiogenesis model of murine Matrigel plug assay. These results demonstrate that clotrimazole is a potent inhibitor of angiogenesis.     

慢性乙型肝炎舌下脉络曲张与辨证分型的关系

通过对242例慢性乙型肝炎患者舌下脉络的观察，据中医辨证分型，发现慢性乙型肝炎不同证型间舌下脉络曲张程度各有差异，肝郁脾虚、脾肾阳虚型舌下脉络迂曲扩张程度均低于瘀血阻络型（经统计学处理，均P＜0．01）。说明舌下脉络迂曲扩张可作为血瘀证辨证的主要指标之一。     

电针在脊髓损伤修复中的应用基础研究概况

本文就电针对脊髓损伤修复作用的应用基础研究进展作一概述,共综合32篇文献的内容,从电针治疗脊髓损伤的方法、取穴部位、应用基础研究现状、电针治疗的作用机制等方面做了归纳总结,为临床应用电针治疗脊髓损伤提供实验依据。     

急性白血病患者血清bFGF与TSGF水平的研究

目的探讨急性白血病(AL)患者血清碱性成纤维细胞生长因子(bFGF)及恶性肿瘤相关物质群(TSGF)的水平及其临床意义。方法应用酶联免疫法(ELISA)及化学显色法分别对47例AL初治(AL初治组)、8例AL复发(AL复发组)患者化疗前后血清bFGF及TSGF的水平进行检测,并与正常人对照组比较;47例AL初治患者化疗后,根据疗效观察,其中11例CR(完全缓解组,CR组)、13例NR(未缓解组,NR组),对CR组与NR组患者血清bFGF及TSGF的水平进行对比分析。结果AL初治组及AL复发组血清bFGF水平均明显高于正常人对照组水平,有非常显著性差异(t=6.71、6.18,P均<0.001);AL初治组及AL复发组血清TSGF水平均明显高于正常人对照组水平,差异非常显著(t=6.847、5.009,P均<0.001);CR组血清bFGF水平及TSGF水平均明显下降,与正常人对照组相比较,均无显著性差异(t=1.11、1.482,P均>0.05);ALLNR组化疗前血清bFGF水平明显高于ALLCR组化疗前的水平,具有显著性差异(t=2.43,P<0.05),AMLNR组与AMLCR组化疗前血清bFGF水平无显著性差异(t=0.76,P>0.05);ALL与AMLNR组化疗前血清TSGF水平均高于CR组化疗前的水平,且均具有显著性差异(t=3.29,2.13;P<0.01,0.05);血清bFGF水平与TSGF水平呈正相关(γ=0.5263,P<0.01)。结论联合检测血清bFGF及TSGF水平对了解AL的发生、发展     

辨证掌握穴位注射方法提高穴位注射的疗效

目的:提高中西结合治疗疾病的疗效.方法:在中医辨证的基础上,在腧穴上进行针剂注射.结果:通过针刺和药液渗透,将穴位疗效和药理作用结合在一起,充分发挥其综合效能.结论:用中医辨证理论,穴位注射治疗能疏通经络,调补阴阳.针药结合,提高疗效.     

Comparison of cystoscopic and histological findings in patients with suspected interstitial cystitis

PURPOSE: Although the exact etiology of interstitial cystitis remains elusive, bladder inflammation appears to be common in many patients. The National Institutes of Health (NIH) have established diagnostic criteria for interstitial cystitis based on the presence of irritative voiding symptoms in the absence of other identifiable pathology. Cystoscopic examination with hydrodistention performed in patients under anesthesia is part of the NIH diagnostic criteria. We determine if the severity of cystoscopic findings correlated with histological evidence of inflammation in patients with suspected interstitial cystitis. MATERIALS AND METHODS: A total of 69 patients who met NIH symptom criteria for interstitial cystitis and underwent cystoscopy, hydrodistention and bladder biopsy under anesthesia were reviewed. There were 2 investigators blinded to the histological data who independently reviewed operative reports. A urological pathologist blinded to the clinical data reviewed biopsies for inflammation severity. Cystoscopic and histological findings were then converted to a numeric scale. Numeric data were analyzed using the Pearson correlation coefficient. RESULTS: Cystoscopic examination revealed no evidence of interstitial cystitis in 6 patients (9%), mild changes in 27 (39%), moderate changes in 23 (33%) and severe changes in 13 (19%). Histological examination revealed no inflammation in 21 patients (30%), mild inflammation in 28 (41%), moderate inflammation in 11 (16%) and severe inflammation in 9 (13%). Histological scores correlated poorly with total and scaled cystoscopic severity scores (r = 0.295 and 0.349, respectively). CONCLUSIONS: Severity of cystoscopic findings observed during hydrodistention with anesthesia does not appear to correlate with the degree of inflammation identified histologically in patients with suspected interstitial cystitis.     

Scoring the short form ICSmaleSF questionnaire. International Continence Society

PURPOSE: The International Continence Society (ICS) ICSmale questionnaire was devised to provide a thorough evaluation of the occurrence and bothersomeness of lower urinary tract symptoms and their impact on the lives of men with benign prostatic disease. This report completes the developmental work on the questionnaire, producing the concise short form instrument, ICSmaleSF, with a valid, reliable and scientifically justified scoring system. MATERIALS AND METHODS: Two data sets were used. The short form version of the questionnaire was devised and initially evaluated using data on men with uncomplicated lower urinary tract symptoms who were involved in the CLasP randomized controlled trial comparing laser therapy with transurethral prostatic resection and conservative management or monitoring without active intervention. External validation of the scoring system was undertaken using data from phase II of the ICS benign prostatic hyperplasia (BPH) study, an observational study of outcome in men with lower urinary tract symptoms related to benign prostatic enlargement. All patients completed the developmental version of the ICSmale questionnaire. Parallel analysis on the CLasP data set identified items that were responsive to change or highly problematic, allowing other redundant and overlapping items to be eliminated. Factor analysis and Cronbach's alpha coefficients were used to examine the clustering of items. Regression models were used to investigate the validity of followup scores within and across treatment groups in the CLasP and ICS/BPH studies. RESULTS: The questionnaire, which originally comprised 22 items, was shortened to 11 items in the 2 distinct factors of voiding (ICSmaleVS) and incontinence (ICSmaleIS) symptoms. Cronbach's alpha coefficients were high at 0.76 for ICSmaleVS and 0.78 for ICSmaleIS. A simple additive score was calculated by adding the 5 items in ICSmaleVS and 6 in ICSmaleIS. ICSmaleVS and ICSmaleIS detected expected improvement in the laser therapy and transurethral prostatic resection groups, and stability in the conservative management group within CLasP. Similarly each subscore but particularly ICSmaleVS was sensitive to differences in the outcome of the range of treatments in the ICS/BPH study. While frequency and nocturia were highly problematic and sensitive to change individually, they did not load into the other main factors or correlate with each other. It is suggested that these symptoms should be evaluated separately with the additional inclusion of a single item measure of the impact of lower urinary tract symptoms on daily life. CONCLUSIONS: The ICSmaleSF represents a comprehensive, concise, valid and reliable instrument for evaluating men with lower urinary tract symptoms. Unlike other questionnaires in the field it contains subscores for the domains of voiding and incontinent symptoms as well as the separate consideration of frequency, nocturia and impact on daily life. We hope that it will become the tool of choice for the comprehensive evaluation of treatment of men with lower urinary tract symptoms associated with benign prostatic disease.     

防范医疗纠纷 提高病案基础质量

防范医疗纠纷 ,必须提高病案基础质量和医疗工作者意识 ,认清病案基础质量存在的主要问题 ;对存在问题进行改进 ;保证病案基础质量 ,维护自己和医院合法权益。