Place de l’épreuve d’effort chez le sportif

Is the stress test still part of the useful examinations in the detection of risk heart disease in sports subjects? Should we continue to use it as a surveillance examination in coronary sports patients? Meta-analysis, considering a ST subshift of more than 1 mm as a criterion for positivity, underline the low prevalence of abnormal examinations and a significant rate of false-positives. But for the diagnosis of coronary artery disease, it is mainly the evolution of the ST segment to effort or recovery and the occurrence of stress arrhythmias that detect true positivity. When coronary risk is more important (presence of risk factors, resumption of sport, intense and prolonged efforts), it seems lawful to recommend such a review on a regular basis among men over 40 years of age with two risk factors but Also among veterans taking up sport after a period of prolonged inactivity. For the asymptomatic coronary athlete the stress test remains at the centre of the final decision for the resumption of a sport in competition irrespective of the method of revascularization. Of course the stress test, even inevitable, remains flawed in the detection of coronary artery disease in the asymptomatic athlete. However, it provides additional information on the condition of being maximal, and attaching importance to arrhythmias, the tensional profile and the maximum power developed in addition to the ST segment's only study.     

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??Objective    To evaluate the cytotoxicity?? biocompatibility and biological safety of PGMA pre-impregnated quartz fiber in vitro?? providing scientific basis for clinical application. Methods    According to standard of GBT16886.5-2003 and YYT0268-2008 documents?? the cellular cultivation and cytotoxicity test in vitro were conducted to evaluate the target materials on the morphology?? growth and proliferation of cultured cells ??L929 and GE1??. Results        The range of cell relative growth rate ??RGR?? of 50% and 100% extraction of non-polymerized composite were 44.59%-65.66% ??L929?? and 52.06%-62.86% ??GE1???? and the cytotoxicity was grade 2-3. The range of cell relative growth rate ??RGR?? of 50% and 100% extraction of polymerized composite were 90.56%-100.89% ??L929?? and 89.99%-101.21% ??GE1???? and the cytotoxicity was grade 0-1. Conclusion    Polymerized PGMA pre-impregnated quartz fiber is safe and fit for the clinical application.     

Murine Model for Contact Sensitization

A method combining murine ear swelling and lymph node cell proliferation was evaluated as a quantitative measurement of delayed-contact sensitization. The method involves one and/or four occluded, abdominal induction applications; use of pertussis toxin as an adjuvant; topical challenge to the ear; baseline and postchallenge ear measurements; and measurement of ¹²⁵I incorporation in axillary lymph nodes after injection of 5-[¹²⁵I]iodo-2'-deoxyuridine ([¹²⁵I]UdR). The inclusion of vehicle-induced/test chemical-challenged control groups permits objective recognition of positive responses by statistical analysis. This model—intended for use in screening for the sensitization potential of topical therapeutics—was evaluated with the known sensitizers tetracaine, naloxone, and oxazolone and with the irritant sodium dodecyl sulfate. Axillary lymph node cell proliferation measured by ¹²⁵I incorporation was a more sensitive measurement of contact sensitization than ear swelling. It was essential to perform both one and four inductions because tetracaine gave the greatest lymph node response after one induction, whereas naloxone produced a sensitization response only after four inductions. Ear swelling response to tetracaine increased after four inductions; however, ear swelling response was not observed after either one or four inductions of naloxone. After one or four inductions of the strong sensitizer, oxazolone, lymph node and ear swelling responses were observed. As expected, no significant ear swelling or lymph node response to sodium dodecyl sulfate was observed after one or four inductions. This method allows quantitative evaluation of contact sensitization by two separate measurements, thereby increasing the potential of identifying sensitizers missed by other methods.     

聚丁二酸丁二醇酯浸提液对人外周血单核细胞的体外遗传毒性

目的： 研究生物可降解材料聚丁二酸丁二醇酯[poly(butylene succinate)，PBS]浸提液对人外周血单核细胞(peripheral blood mononuclear cells，PBMCs)的体外遗传毒性。方法：参照GB/T 16886-2005标准制备PBS浸提液。采用MTT试验评价不同浓度(200、100、50、25、12.5、6.25、3.125 mg/mL) PBS浸提液对PBMCs的细胞毒性；并进行染色体畸变试验和微核试验评价其体外遗传毒性。同时设溶剂RPMI-1640和医用级左旋聚乳酸(L-polylactide，L-PLA)对照组。结果：PBS浸提液对PBMCs的细胞毒性与溶剂对照和L-PLA比较差异均无统计学意义(P＞0.05)；200 mg/mL PBS浸提液在代谢活化和非代谢活化条件下与PBMCs共培养48 h后染色体畸变均为阴性；200 mg/mL PBS浸提液诱发的微核细胞数与溶剂对照组和L-PLA组相比差异均无统计学意义(P＞0.05)。结论：在本试验条件下，生物可降解材料PBS浸提液在体外对PBMCs无遗传毒性，其影响与医用级L-PLA相当。     

射波刀验收检测方法及思考

介绍新一代四维放射外科治疗设备射波刀（Cyberknife）的组成、主要参数及检测方法。射波刀以其亚毫米级的治疗精度,机械臂6个方向的运动维度,较常规放疗设备具有较大优势。笔者通过对射波刀实际的验收检测,总结其验收方法,并结合实际情况引发对射波刀验收检测工作的思考。     

佛山市禅城区中小学生肺结核感染和患病现况

目的了解佛山市禅城区中小学生结核菌感染和肺结核患病现况,为主管部门提供学校结核病防治工作决策依据。方法整群抽取佛山市禅城区中小学新生19 685名进行PPD试验,检查结果采用SPSS 16.0统计分析。结果佛山市中小学生PPD总体阳性率为52.0%,强阳性率3.6%,发现5例活动性肺结核病人,患病率为28.76/105。初中学生PPD阳性4 737例(51.5%),强阳性307例(3.3%);小学生阳性3 689例(45.1%),强阳性311例(3.8%);初中生阳性率低于小学生(χ2=70.36,P<0.01)。受检男生中,阳性4459例(46.1%),强阳性255例(2.6%);女生中,阳性3 967例(51.4%),强阳性363例(4.7%),女生阳性率高于男生(χ2=124.39,P<0.01)。有肺结核患者的学校PPD阳性和强阳性检出率高于无肺结核患者的学校(χ2=31.76,P<0.01),患者所在班级PPD总体阳性率高于无患者的班级(χ2=9.28,P=0.01)。结论佛山市中小学学生结核菌感染率和活动性肺结核患病率较高,初中生、女生易感,患者所在班级和学校结核菌感染率较高。因此,要针对重点人群,加强佛山市中小学生结核病防治工作。     

平板运动试验阳性的女性患者冠脉造影结果分析

目的 探讨女性平板运动试验(TET)假阳性的原因及颈动脉斑块检查对冠心病无创性诊断的意义.方法 对96例TET阳性的女性患者进行冠脉造影(CAG)及颈动脉斑块检查,对三种检查结果进行比较,回顾性分析女性患者出现平板运动试验假阳性的原因.结果 CAG证实为冠心病(CHD)58例(占60.4%),非CHD 38例(占39.6%);颈动脉斑块检查阳性者71.2%被证实为CHD,而阴性者仅36.7%被证实为CHD;非CHD 38例中临床最后诊断:心血管神经症20例,高血压病4例,单纯血脂异常4例,糖尿病3例,X综合征2例,高血压合并糖尿病2例,冠状动脉心肌桥2例,心尖部肥厚型心肌病1例.结论 平板运动试验假阳性与心血管神经症、X综合征、冠状动脉肌桥、高血压病、糖尿病等因素有关,心血管神经症为最常见病因.TET结合颈动脉斑块检查可显著提高冠心病无创性检查的诊断率.     

CAT和HAD评价慢性阻塞性肺疾病稳定期患者焦虑、抑郁状态相关的比较

目的分析稳定期慢阻肺疾病患者的HAD和CAT相关性。方法从740例稳定期慢阻肺患者中,通过问卷形式,统计患者HAD和CAT评分,对于符合HAD》11分,同时满足排除标准的患者在进行统计相关性分析。结果共有74人符合本次研究的规定,其焦虑、抑郁发病率为10%。当中,男性56人,年龄(67士12)岁,焦虑、抑郁发病率:9.6%;女性18人,年龄(75士10)岁,焦虑、抑郁发病率:11.25%。焦虑和抑郁评分作为因变量,通过Pearson分析:其中CAT组相关系数r分别为0.76、0.61,P0.01,提示CAT和HAD呈强正相关,年龄组r=0.243,P=0.062;r=0.156、P=0.189,和HAD无统计学意义;FEV_1组相关系数r=-0.3、P0.05;r=-0.25、P0.05,提示和HAD呈弱负相关。结论 CAT评分和HAD评分有着密切的关联,既可以通过它反应患者的焦虑、抑郁等心理状态,又能反应慢阻肺患者健康相关生活质量,临床上操作简单,同时患者接受度高。     

Occurrence of Flavonol Sulphates in Alchornea laxiflora

For the first time, quercetin-3',4'-disulphate (1) and another novel flavonol sulphate quercetin-7,4'-disulphate (2) were isolated from an ethyl acetate-soluble fraction of the crude methanol extract of Alchornea laxiflora. Purification of these compounds was carried out by column chromatography utilising Sephadex LH 20 with 30% toluene in ethanol as eluent. Structural elucidation was by UV, IR, 1 H-NMR and 13 C-NMR spectroscopy as well as by FAB-MS.