高原低氧对大鼠学习记忆影响的实验研究

目的：研究高原低氧环境对大鼠学习记忆的影响。方法：利用新生大鼠低氧模型和胎鼠低氧模型，采用爬杆实验和Morris水迷宫实验进行检测.结果：爬杆实验中习得的速度减慢，消退速度加快。Morris水迷宫实验中逃避潜伏期延长，撤除平台后跨越平台次数减少。结论：高原低氧环境下大鼠的学习记忆能力受到抑制，可能与长期低氧后海马NMDA受体数量和功能的下降有关。     

5种真空采血促凝管在生化检验中的应用及评价

目的 评价五种真空采血促凝管对常规生化检测项目的影响。方法 采集健康体检者及自愿者血液标本100例,选用5个不同厂家生产的真空采血促凝管,与美国BD公司生产的无添加剂的血清管进行采血质量和生化检测对比评价。结果 5个厂家生产的真空采血促凝管与BD公司生产的无添加剂血清管在多数生化检验项目上无明显差异(P>O.05),但有3个国内厂家生产的真空采血促凝管在标本采集质量、分离效果上存在明显不足,且在血清K、LDH、HBD、CK、CHOL、TG、HDL-c、LDL-c、APOA、APOB等检测上与对照组间存在着显著差异(P<0.05且r相似文献   

股骨干骨折扩髓腔髓内钉固定对凝血机能和肺换气功能的影响

【目的】探讨股骨干骨折扩髓与不扩髓髓内钉固定对凝血机能和肺换气功能的影响。【方法】 38例股骨干骨折分别采用扩髓 (RFN)和不扩髓 (UFN)髓内钉固定 ,在手术开始前至术后 4 8h内 7个不同时间段监测血小板、纤维蛋白原、凝血酶原时间 (PT)和部分凝血活酶激活时间 (APTT)的变化 ,并动脉血气分析 ,计算肺泡 动脉血氧分压递差 [P(A aDO2 ) ]。【结果】RFN组 ,扩髓后血小板开始减少 ,P(A aDO2 )升高 (P<0 0 5 ) ;髓钉插入后 30min纤维蛋白原减少 ,PT、APTT延长 (P <0 0 5 ) ;UFN组表现出同样的趋势 ;两组中各项指标均在 2 4～ 4 8h内恢复正常 ;动脉血气分析各时间段和组间差异无显著性 (P >0 0 5 )。【结论】扩大髓腔的髓内钉固定 ,和不扩髓相比 ,并不影响肺换气功能 ,对凝血机能的影响是一个自限过程 ,单纯股骨干骨折 ,可耐受扩髓固定手术。     

Behavioral models in mice. implication of the alpha noradrenergic system

1. The mechanism of action of drugs might change according to the test used. Several noradrenergic drugs were tested in order to understand their implication in the mobility tests.

2. It was found that clonidine, an Alpha 2 agonist, acted differently according to the tast used. It provoked sedation in spontaneous activity test, and anti-immobility effects in the other tests.

3. Tall suspension test is able to show the double acting of clonidine.

4. Idazoxan might act either as an alpha 2 antagonist or as partial alpha 2 agonist. TST shown the unexpected partial alpha agonist effect of the molecule.

5. Forced swimming test is more specific for predicting antidepressant activity than tail suspension test which is close to a spontaneous activity model.     

出国务工妇女细菌性阴道病调查结果分析

目的:了解细菌性阴道病在延边地区出国务工妇女中的流行情况。方法:用改良多胺法对延边地区586名出国务工妇女进行细菌性阴道病(BV)检测,同时用常规方法进行滴虫、霉菌检查。结果:586份阴道分泌物标本中,检出BV(+)275例,总阳性率为46.9%,其中单纯BV(+)242例(41.3%)、BV合并滴虫(+)8例(1.3%)、BV合并霉菌(+)25例(4.3%);按年龄分组,33～35岁组BV阳性率最高,为60.3%,≤20岁组最低,为20.0%。结论:延边地区出国务工妇女细菌性阴道病患病率较高,居该人群各种阴道感染的首位,无民族差异(χ2=0.22,P>0.05),但年龄差异显著(χ2=28.72,P<0.05)。因此,建议在出国体检中常规开展BV检查,以便及时发现和治疗,控制疾病传播范围。     

JX-1对雄性小鼠生殖细胞致突变作用的研究

本文采用小鼠精子试验和小鼠睾丸DNA合成抑制试验,检测了JX-1对雄性生殖细胞的致突变作用。实验结果表明JX-1对小鼠精子总数、精子活动率及精子畸形率均无统计学意义的增加,与小鼠睾丸DNA合成抑制试验阴性反应一致。作者认为JX-1对雄性生殖细胞为一种非诱变剂。     

乳腺积乳囊肿的诊断与治疗

本文报告乳腺积乳囊肿43例。讨论了发病原因及病理,特别提出局部肿块波动感试验阳性,A超检查显示液平段,试穿抽出乳汁,对诊断有肯定价值;治疗以手术切除为主,仅有7例穿刺抽吸乳汁得以治愈。     

Conditional estimation of sensitivity and specificity from a phase 2 biomarker study allowing early termination for futility

Development of a disease screening biomarker involves several phases. In phase 2 its sensitivity and specificity is compared with established thresholds for minimally acceptable performance. Since we anticipate that most candidate markers will not prove to be useful and availability of specimens and funding is limited, early termination of a study is appropriate, if accumulating data indicate that the marker is inadequate. Yet, for markers that complete phase 2, we seek estimates of sensitivity and specificity to proceed with the design of subsequent phase 3 studies. We suggest early stopping criteria and estimation procedures that adjust for bias caused by the early termination option. An important aspect of our approach is to focus on properties of estimates conditional on reaching full study enrollment. We propose the conditional‐UMVUE and contrast it with other estimates, including naïve estimators, the well‐studied unconditional‐UMVUE and the mean and median Whitehead‐adjusted estimators. The conditional‐UMVUE appears to be a very good choice. Copyright © 2008 John Wiley & Sons, Ltd.     

Successful Liver and Kidney Transplantation From Cadaveric Donors With Left-Sided Bacterial Endocarditis

Bacterial infections are frequent in cadaveric organ donors and can be transmitted to the transplantation recipient, which could have devastating consequences for the recipients if adequate preventive measures are not adopted.
From the 355 consecutive brain dead cadaveric organ donors procured at our center in the last four years, 2000–2003, four of them (1.1%) had bacterial endocarditis as cause of death. The bacteria responsible for the endocarditis were Staphylococcus epidermidis, coagulase-negative Staphylococcus , Staphylococcus hominis and Streptococcus viridans , respectively. We performed five kidney and two liver transplantations on seven recipients. All donors and recipients received antibiotic treatment against the germ causing the respective endocarditis.
Infection by the bacteria responsible for the endocarditis in the respective donors was not transmitted to any of the recipients. Six of the seven recipients were alive with normal-functioning grafts after between 13 and 24 months' follow-up. Transplantectomy was performed on one kidney recipient due to thrombosis of the renal vein of the graft not related to the endocarditis.
Liver and kidney transplantation from donors dying from bacterial endocarditis can be performed without causing the transmission of infection to the recipient or the dysfunction of the graft.