绿健运动饮料对运动员某些生理指标的影响

目的:了解绿健运动饮料在大运动训练条件下对拳击、跆拳道运动员某些生理指标的影响,探讨其是否具有提高人体体能和抗疲劳功能.方法:将拳击、跆拳道运动员随机等分为实验组和对照组,实验组和对照组每天分别补充绿健运动饮料和对照饮料,实验前、2及4周时对受试对象的血液生化指标进行测试.结果:实验组与对照组比较,血清睾酮水平显著升高(P＜0.05),肌酸激酶显著下降(P＜0.05),血红蛋白含量相对稳定.结论:补充绿健运动饮料有利于拳击、跆拳道运动员在大强度训练时机体功能的提高和疲劳的恢复.     

哈蟆油抗疲劳药理活性研究

目的研究哈蟆油的抗疲劳作用。方法选用雄性BALB/C小鼠,设高、中、低3个剂量组和空白对照组,给不同剂量的哈蟆油30d后。观察小鼠负重游泳时间、血清尿素氮含量、肝糖原含量以及游泳前后血乳酸含量的变化。结果与对照组比较,实验组小鼠负重游泳时间的延长差异有统计学意义(P﹤0.05),肝糖原含量的增加差异有统计学意义(P﹤0.05和P﹤0.01),血乳酸曲线下面积的减小差异有统计学意义(P﹤0.01),血清尿素氮含量的降低差异无统计学意义(P﹥0.05)。结论哈蟆油具有明显的抗疲劳作用。     

藏药雪茶提取物抗疲劳作用的实验研究

探讨雪茶提取物[Extractive of Thamnolia vermicularis（ACH）Asahina]的抗疲劳作用效果。方法：取小鼠,随机分组,实验设高、低剂量组分、空白组、阳性药物组。雪茶提取物应用灌胃给药方法,通过各组小鼠负重游泳实验,对雪茶的抗疲劳作用进行物理性研究;通过测试小鼠游泳后和静置一定时间后血液中血糖变化,血乳酸变化,肝糖原含量及血尿素含量,对雪茶的抗疲劳作用进行相关生理指标研究。结果：雪茶提取物显著增加了小鼠的游泳时间,血液和肝脏中各个指标的变化具有良性效果。结论：雪茶提取物有提高小鼠的抗疲劳作用的效果,并在一定浓度范围内呈剂量依赖性显著改善小鼠抗疲劳能力。     

养血生乳口服液的药效学研究

目的探讨养血生乳口服液的药理作用。方法观察养血生乳口服液对产后大鼠及急性失血小鼠Hb和RBC的影响,及对小鼠产后乳汁分泌量和游泳时间的影响。结果产后大鼠及急性失血小鼠Hb和RBC明显降低,经养血生乳口服液治疗后,动物Hb和RBC显著升高,母鼠在产后第4天和第10天的泌乳量明显升高,小鼠游泳时间明显延长。结论养血生乳口服液有明显的补血、抗疲劳和促进产后乳汁分泌作用。     

红景天含片缓解小鼠体力疲劳作用的研究

[目的]研究红景天含片的抗疲劳保健功能。[方法]给小鼠灌胃该含片低、中、高3个剂量组,同时设立对照组,观察小鼠体重、负重游泳时间、肝糖原含量、血乳酸等指标的变化。[结果]中、高剂量组含片的小鼠负重游泳时间(s)分别为635±212、660±247,与对照组377±100比较明显延长;小鼠肝糖原含量中剂量组为4.54±0.83(g/100 g肝组织),与对照组3.27±0.77(g/100 g肝组织)比较有显著增加;高剂量组小鼠运动前后血乳酸曲线下面积为85.3±7.5,与对照组112.9±13.3比较差异有统计学意义(P<0.01)。[结论]该含片能明显延长小鼠负重游泳时间,显著增加小鼠肝糖原含量,减少小鼠运动前后血乳酸曲线下面积,具有缓解体力疲劳功能。     

龙胆草提取物抗炎、镇痛、耐缺氧及抗疲劳作用的研究

目的：探讨龙胆草提取物抗炎、镇痛、耐缺氧及抗疲劳作用。方法：160只小鼠随机分成4组：对照组，龙胆草大、中、小剂量组，龙胆草提取物灌胃给药，对照组以等体积生理盐水灌胃。采用二甲苯致小鼠耳壳肿胀的方法，测定耳片的重量差；用冰醋酸致小鼠的扭体次数，测定小鼠的扭体反应次数：采用常压耐氧法记录小鼠耐缺氧时间；采用比色分析法测定游泳45min后小鼠肝糖原及血清乳酸含量。结果：龙胆草提取物对二甲苯所致小鼠耳壳肿胀有抑制作用，能减少冰醋酸所致小鼠的扭体次数，延长缺氧情况下的存活时间，小鼠游泳45min后肝糖原的含量明显增加、乳酸含量明显减少，与对照组比较均有显著性差异。结论：龙胆草提取物具有一定的抗炎、镇痛、耐缺氧及抗疲劳作用。     

复方熊胆制剂抗疲劳作用研究

目的 探讨复方熊胆制剂的抗疲劳作用,为临床用药提供可靠的实验室依据.方法 以小鼠为实验对象,给予不同剂量的复方熊胆制剂灌胃饲养,观察复方熊胆制剂对小鼠游泳时间、爬杆时间及游泳后肝糖元、血清尿素氮、血乳酸、乳酸脱氢酶等生化指标.结果 复方熊胆制剂能明显延长小鼠负重游泳时间和爬杆时间,提高小鼠运动后肝糖元含量和血乳酸脱氢酶活力,降低血清尿素氮和血乳酸含量.结论 复方熊胆制剂具有抗疲劳作用.     

Anti-fatigue activity of polysaccharides from the fruits of four Tibetan plateau indigenous medicinal plants

Ethnopharmacological relevance

The fruits of Hippophae rhamnoides L., Lycium barbarum L., Lycium ruthenicum Murr. and Nitraria tangutorum Bobr. are traditional medicinal food of Tibetans and used to alleviate fatigue caused by oxygen deficiency for thousands of years. The present study focused on exploiting natural polysaccharides with remarkable anti-fatigue activity from the four Qinghai-Tibet plateau characteristic berries.

Materials and methods

The fruits of Hippophae rhamnoides, Lycium barbarum, Lycium ruthenicum and Nitraria tangutorum were collected from Haixi national municipality of Mongol and Tibetan (N 36.32°, E98.11°; altitude: 3100 m), Qinghai, China. Their polysaccharides (HRWP, LBWP, LRWP and NTWP) were isolated by hot-water extraction, and purified by DEAE-Cellulose ion-exchange chromatography. The total carbohydrate, uronic acid, protein and starch contents of polysaccharides were determined by a spectrophotometric method. The molecular weight distributions of polysaccharides were determined by gel filtration chromatography. Their monosaccharide composition analysis was performed by the method of 1-phenyl-3-methyl-5-pyrazolone (PMP) pre-column derivatization and RP-HPLC analysis. HRWP, LBWP, LRWP and NTWP (50, 100 and 200 mg/kg) were orally administrated to mice once daily for 15 days, respectively. Anti-fatigue activity was assessed using the forced swim test (FST), and serum biochemical parameters were determined by an autoanalyzer and commercially available kits; the body and organs were also weighted.

Result

LBWP, LRWP and NTWP were mainly composed of glucans and some RG-I pectins, and HRWP was mainly composed of HG-type pectin and some glucans. All the four polysaccharides decreased immobility in the FST, and the effects of LBWP and NTWP were demonstrated in lower doses compared with HRWP and LRWP. There was no significant difference in liver and heart indices between non-treated and polysaccharide-treated mice, but the spleen indices were increased in LBWP and NTWP (200 mg/kg) group. Moreover, the FST-induced reduction in glucose (Glc), superoxide dismutase (SOD) and glutathione peroxidase (GPx) and increase in creatine phosphokinase (CK), lactic dehydrogenase (LDH), blood urea nitrogen (BUN), triglyceride (TG) and malondialdehyde (MDA) levels, all indicators of fatigue, were inhibited by HRWP, LBWP, LRWP and NTWP to a certain extent while the effects of LBWP and NTWP were much better than that of HRWP and LRWP at the same dosage.

Conclusion

Water-soluble polysaccharides HRWP, LBWP, LRWP and NTWP, from the fruits of four Tibetan plateau indigenous berry plants, significantly exhibited anti-fatigue activities for the first time, through triglyceride (TG) (or fat) mobilization during exercise and protecting corpuscular membrane by prevention of lipid oxidation via modifying several enzyme activities. Moreover, it is demonstrated that LBWP and NTWP are more potent than HRWP and LRWP, which were proposed to be applied in functional foods for anti-fatigue and antioxidant potential.     

复方红景天苷胶囊抗疲劳效果的研究

目的研究复方红景天苷胶囊消除疲劳的效果。方法选取年龄19～24岁军校学员20名,口服复方红景天苷胶囊20 d,采用自身对照的方法,测定体成分、联合功能试验及进行血液生化检查。结果试验前后用体成分分析仪（OLYMPI-A）3.5测定的结果均在正常范围内。联合功能试验包括全蹲起、15 s快跑、3 min高抬腿。服用复方红景天胶囊20 d后,脉搏数减2次,收缩压降低1 mm Hg,差异无统计学意义;血常规、肝肾功能试验前后均为正常;血清乳酸有所降低（P〈0.05）;超氧化物歧化酸（SOD）活力试验后升高（P〈0.01）;丙二醛（MDA）含量试验后降低（P〈0.05）。结论试验采用的复方红景天胶囊剂量是安全的。复方红景天胶囊有明显的消除疲劳的作用,可以提高机体免疫功能和运动能力。     

肉苁蓉与淫羊藿提取物对小鼠抗疲劳作用研究

目的　研究肉苁蓉淫羊藿提取物的抗疲劳作用.方法　采用小鼠转轮停留时间、游泳时间比较肉苁蓉淫羊藿提取物与人参水煎液的抗疲劳作用.结果　空白对照组、人参水煎液组、药物Ⅰ组、药物Ⅱ组的转轮停留时间分别为(71.778±10.587)s、(103.111±8.195)s 、(86.667±6.206)s、(93.889±8.011)s,游泳时间分别为(252.889±20.301)s、(327.111±17.344)s、(304.333±21.147)s、(312.444±16.828)s.结论　肉苁蓉淫羊藿提取物具有显著的抗疲劳作用.