去酰基化胃饥饿素对高脂饮食诱导C57BL/6J小鼠的抗疲劳作用

目的探究去酰基化胃饥饿素(DAG)对高脂饮食(HFD)诱导的肥胖小鼠的抗疲劳作用。方法36只6周龄C57BL/6小鼠随机分为对照(NC)组、高脂(HFD)组、HFD+DAG组(每组各12只),分别予以标准饲料(NC组)、高脂饲料(HFD组、HFD+DAG组)喂养,并给予每日腹腔注射生理盐水(NC组,HFD组)和DAG(HFD+DAG组)。喂养16 w后行力竭测试和负重游泳实验,随后处死小鼠取血检测血清乳酸(LA)、乳酸脱氢酶(LDH)、尿素氮(BUN)水平;留取肝脏和股四头肌测定肝糖原和肌糖原水平。结果与NC组相比,HFD组体重上升,腓肠肌和股四头肌指数下降,力竭运动距离和游泳力竭时间均缩短,血清BUN、LA水平升高,血清LDH、肝糖原、肌糖原含量下降,差异有统计学意义(P<0.05,P<0.01)。与HFD组相比,HFD+DAG组力竭运动距离和游泳力竭时间延长,血清BUN、LA水平下降,血清LDH、肝糖原、肌糖原含量升高,差异有统计学意义(P<0.05,P<0.01)。结论长期应用DAG有助于提高肥胖小鼠的运动耐力与抗疲劳能力。     

黄芪精口服液对大鼠抗疲劳作用研究

目的:观察黄芪精口服液对小鼠抗疲劳能力的影响。方法:选用单一雄性昆明种小鼠36只,随机分为空白对照组(生理盐水)、黄芪精口服液低剂量组(0.05g·10g-1)、高剂量组(0.1g·10g-1),每天按0.07ml·10g-1,0.14ml·10g-1灌胃一次,连续7d,记录小鼠游泳时间,并用分光光度计法检测小鼠剧烈运动后血尿素氮(BUN)含量、超氧化物歧化酶(SOD)活性。以小鼠游泳时间为指标研究其抗疲劳作用。结果:与对照组比较,高剂量黄芪精口服液可显著提高小鼠负重游泳时间,增加血浆SOD活性,降低BUN含量(P0.05)。结论:黄芪精口服液具有提高小鼠抗疲劳能力的作用。     

哈蟆油抗疲劳药理活性研究

目的研究哈蟆油的抗疲劳作用。方法选用雄性BALB/C小鼠,设高、中、低3个剂量组和空白对照组,给不同剂量的哈蟆油30d后。观察小鼠负重游泳时间、血清尿素氮含量、肝糖原含量以及游泳前后血乳酸含量的变化。结果与对照组比较,实验组小鼠负重游泳时间的延长差异有统计学意义(P﹤0.05),肝糖原含量的增加差异有统计学意义(P﹤0.05和P﹤0.01),血乳酸曲线下面积的减小差异有统计学意义(P﹤0.01),血清尿素氮含量的降低差异无统计学意义(P﹥0.05)。结论哈蟆油具有明显的抗疲劳作用。     

黑加仑提取物抗疲劳作用与GAT-2、Scd-2基因的关系

目的探讨黑加仑提取物对疲劳小鼠脑组织中γ-氨基丁酸转运体2(GAT-2)、硬脂酰基-辅酶A去饱和酶2(stearoyl-CoA desaturas-2,Scd-2)基因表达的影响。方法选择健康BALB/c雄性小鼠随机分为运动+服药组、运动组、安静对照组,建立运动性疲劳模型后,运用RT-PCR技术对小鼠脑组织中的基因表达进行分析。结果运动+服药组脑组织中GAT-2、Scd-2基因的光亮度较运动组和安静对照组高(P0.05)。结论在本试验条件下,黑加仑提取物可消除大强度训练过程中γ-氨基丁酸(GABA)诱发的疲劳作用,其作用亦与脑组织中的Scd-2基因表达有关联。     

Drug release behavior of chitosan-montmorillonite nanocomposite hydrogels following electrostimulation

Nanocomposites hydrogel (nanohydrogel) composed of chitosan (CS) and montmorillonite (MMT) were prepared and systematically studied for drug release behavior following electrostimulation. The deterioration of the responsiveness and reversibility of CS upon repeated on-off electrostimulation switching operations are major limitations for clinical applications, as it suffers from too much structural instability for the precise control of the release of drug upon cyclic electrostimulation. To overcome these limitations, an inorganic phase, MMT, was incorporated in the CS matrix to enhance the anti-fatigue property and corresponding long-term stable release kinetics. X-ray diffraction analysis and time-dependent optical absorbance showed that the MMT incorporated into the nanohydrogel exhibited an exfoliated nanostructure. The exfoliated silica nanosheets are able to act as cross-linkers to form a network structure between the CS and MMT, and this difference in the cross-linking density strongly affects the release of vitamin B(12) under electrostimulation. With a lower MMT concentration (1 wt.%), the release kinetics of vitamin B(12) from the nanohydrogel shows a pseudo-zero-order release, and the release mechanism was changed from a diffusion-controlled mode to a swelling-controlled mode under electrostimulation. Further increasing the MMT content reduced both the diffusion exponent n and the responsiveness of the nanohydrogel to electrostimulation. In addition, a consecutively repeated "on" and "off" operation shows that the electroresponsiveness of the nanohydrogel with higher MMT concentrations was reduced, but its anti-fatigue behavior was considerably improved. In this work, the nanohydrogel with 2 wt.% MMT achieved a mechanically reliable and practically desirable pulsatile release profile and excellent anti-fatigue behavior, compared with that of the pure CS.     

黄连素抗疲劳作用相关因素分析

目的 研究黄连素在军事训练中的抗疲劳作用。方法 野外训练后第3天口服黄连素0．3g，3次／d，观察学员发病状况。结果 对照组1124人，出现上呼吸道感染125人，腹泻149人，踝关节扭伤后肿胀56人，发热18人，腰腿痛256人，奔袭中脑病12人。治疗组1125人，出现上呼吸道感染88人，腹泻12人，踝关节扭伤后肿胀52人，发热10人，腰腿痛126人，奔袭中脑病7人。结论 黄连素对预防上呼吸道感染、腹泻、发热、腰腿痛、奔袭中脑病有明显抗疲劳作用。     

三康胶囊抗疲劳作用与血液生化指标的改变

目的探讨三康胶囊(SKC)抗疲劳作用与血液生化指标改变的关系。方法灌胃给予高、低剂量的SKC,(1.5g/kg、0.5g/kg),采用小鼠疲劳爬杆实验评价药物的抗疲劳效果。同时,观察游泳疲劳状态下相关的代谢产物如血乳酸、血尿素氮含量的变化。结果与爬杆对照组相比,SKC高、低剂量组分别延长爬杆时间42.98%(P<0.05)和14.77%;SKC还可升高外周血红细胞数和血红蛋白含量。与游泳模型组相比,SKC高剂量组可增加疲劳状态下血浆中乳酸脱氢酶(LDH)活性(6.62±0.37vs6.08±0.38,P<0.05),减少血乳酸含量(9.45±1.38vs11.68±1.20,P<0.05),降低长时间运动后升高的血尿素氮(13.24±1.86vs16.51±2.14,P<0.05)。结论SKC具有抗疲劳的作用,其机制可能与血液生化指标的改善有关。     

龙胆草提取物抗炎、镇痛、耐缺氧及抗疲劳作用的研究

目的：探讨龙胆草提取物抗炎、镇痛、耐缺氧及抗疲劳作用。方法：160只小鼠随机分成4组：对照组，龙胆草大、中、小剂量组，龙胆草提取物灌胃给药，对照组以等体积生理盐水灌胃。采用二甲苯致小鼠耳壳肿胀的方法，测定耳片的重量差；用冰醋酸致小鼠的扭体次数，测定小鼠的扭体反应次数：采用常压耐氧法记录小鼠耐缺氧时间；采用比色分析法测定游泳45min后小鼠肝糖原及血清乳酸含量。结果：龙胆草提取物对二甲苯所致小鼠耳壳肿胀有抑制作用，能减少冰醋酸所致小鼠的扭体次数，延长缺氧情况下的存活时间，小鼠游泳45min后肝糖原的含量明显增加、乳酸含量明显减少，与对照组比较均有显著性差异。结论：龙胆草提取物具有一定的抗炎、镇痛、耐缺氧及抗疲劳作用。     

Anti-fatigue effects of salidroside in mice

Objective： To study the anti-fatigue effects of salidroside in mice. Methods： Totally 120 normal male Kunming mice were randomized into 5 groups （4 salidroside intervention groups and the control group） based on body weight. The control group was given distilled water and the 4 intervention groups were given various doses of salidroside （60, 180, 360, 720 mg/kg） for 15 consecutive days, respectively. The levels of lactate, serum urea nitrogen, muscle and liver glycogen, the longest swimming time and hemoglobin were determined before and after swimming test. Results： Different doses of salidroside significantly lengthened the swimming time and increased the contents of hemoglobin and muscle and liver glycogen, while reducing that of lactate in blood significantly compared with control group, especially in the 180 mg/kg salidroside group. Conclusion： Salidroside has noticeable anti-fatigue effect on mice. These effects were dose-dependent, and the strongest effect on most biomarkers was seen with an intermediate dose.     

TPSH保健品抗疲劳作用的初步实验研究

目的初步探讨以红景天、刺五加为主要成份的TPSH保健品的抗疲劳作用。方法将雄性KM小鼠按随机分为4组：即空白对照组和0．12、0．24、0．72 g/kg·bw3个给药剂量组。在灌胃给予受试物30 d后测定各组小鼠负重游泳的持续时间、血清尿素水平、肝糖原含量及血乳酸水平的变化。结果0．72 g/kg·bw剂量能延长小鼠的负重游泳时间、显著降低小鼠运动后血清尿素水平和血乳酸曲线下面积(P＜0．05),肝糖原储备量无明显变化。结论TP- SH保健品具有抗疲劳作用。