石门穴临床应用举隅

介绍石门穴的局部解剖、针灸方法、主治功效及其对尿路感染、小儿遗尿、妇女避孕等验案四则。     

食管癌中CD44 V5和nm23-H1基因表达及意义

目的 :探讨CD4 4V5,nm2 3 H1 的基因产物表达与食管癌临床病理及预后的关系。方法 :应用免疫组织化学S P法对 85例食管癌标本进行CD4 4V5和Nm2 3 H1 的基因产物测定 ,并对其中 50例患者术后随访 3年。结果 :①CD4 4V5在食管癌中的阳性表达率为 61 .2 % ,Nm2 3 H1 在食管癌中的阳性表达率为 57.6 %。②在食管癌中CD4 4V5的过表达和Nm2 3 H1 的低表达与食管癌的浸润深度、分化程度、淋巴结转移、临床TNM分期及预后相关 (P<0 .0 5) ,而与食管癌患者的性别、年龄、肿瘤大小、病理类型均无相关性 (P >0 .0 5)。③在食管癌中 ,CD4 4V5和Nm2 3 H1 的表达呈负相关 (r=- 0 439,P <0 .0 1 ) ,且CD4 4V5的过表达伴有Nm2 3 H1 低表达者发生淋巴结转移的可能性大 (P <0 .0 1 )。结论 :CD4 4V5和Nm2 3 H1 在食管癌中的不同表达与食管癌的淋巴结转移及预后显著相关。CD4 4V5、Nm2 3 H1 的不同表达在食管癌淋巴结转移中可能起协同作用 ,可为临床预测食管癌淋巴结转移及评估预后提供重要的参考指标     

5-羟基-1H-吲哚-3-羧酸乙酯类化合物的合成及其抗流感病毒活性

目的设计合成5-羟基-1H-吲哚-3-羧酸乙酯类化合物,评价其抗流感病毒和抗呼吸道合胞病毒活性.方法经IR、1H-NMR和MS确证目标物结构,并经体外抗病毒试验进行活性筛选.结果与结论合成了9个5-羟基-1H-吲哚-3-羧酸乙酯类化合物,初步活性试验表明,具有一定的抑制流感病毒和呼吸道合胞病毒作用,其中,化合物Ⅷ1、Ⅷ2、Ⅷ5的抗病毒活性与利巴韦林和阿比朵尔相当.     

太子健对哮喘豚鼠白细胞介素-5 P-选择素的影响

目的　观察太子健 对反复诱发哮喘的哮喘豚鼠血中白细胞介素 (IL) - 5、黏附分子 P-选择素的影响。方法　将豚鼠随机分为正常组、模型组、地塞米松组、太子健 高剂量和低剂量组 5组 ,后 4组分别用卵蛋白致敏 15 d后 ,再用卵蛋白反复诱发哮喘 ,正常组用生理盐水代替 ,同时 ,正常组和模型组灌服生理盐水 ,其余 3组分别灌服地塞米松、高剂量和低剂量太子健 ,予以治疗 ,连续 15 d。结果　太子健 可减少血中 IL- 5、黏附分子 P-选择素的含量。结论　太子健 可以影响 IL- 5、P-选择素对炎症细胞的调节作用 ,从而减轻气道慢性变应性炎症 ,具有一定的抗哮喘复发的作用。     

5,7双羟色胺对不同功能运动神经元群调质CGRP配布式？…

目的 研究５,７双羟色胺（５,７－ＤＨＴ）对不同功能运动神经元（Ｍｎ）群调质ＣＧＲＰ配布式样的影响。方法 利用霍艉毒素Ｂ亚单位结合胶体取长补短地标记神经元复合化学的非荧光双标记方法。结果 大鼠比目鱼肌（慢肌）Ｍｎ（ＳＯＬ－Ｍｎ）群和趾长伸肌（快ｎ（ＥＤＬ－Ｍｎ）群ＣＧＲＰ配布式样不同,即ＳＯＬ－Ｍｎ群含较高比率（ＣＧＲＰ－ＬＩ）阴性和弱阳性Ｍｎ,ＥＤＬ－Ｍｎ群含较高比率ＣＧＲＰ－ＬＩ强阳性Ｍｎ,于     

Developmental pattern of 3-oxo-Δ4 bile acids in neonatal bile acid metabolism

AIMS—To investigate whether a fetal pathway of bile acid synthesis persists in neonates and infants.
METHODS—3-oxo-Δ⁴ bile acids were determined qualitatively and quantitatively in the urine, meconium, and faeces of healthy neonates and infants, using gas chromatography-mass spectrometry.
RESULTS—The mean percentage of 3-oxo-Δ⁴ bile acids in total bile acids in urine at birth was significantly higher than that at 3 or 7 days, and at 1 or 3 months of age. The concentration of this component in meconium was significantly higher than that in faeces at 7 days and at 1 or 3 months of age.
CONCLUSIONS—The presence of large amounts of urinary 3-oxo-Δ⁴ bile acids may indicate immaturity in the activity of hepatic 3-oxo-Δ⁴-steroid 5β-reductase in the first week of postnatal life. Large amounts of this component in meconium may be due to the ingestion of amniotic fluid by the fetus during pregnancy.

     

Correlation between augmentative effects of leucovorin on 5-fluorouracil and thymidylate synthase inhibition

Background We studied the relationship between the augmentative effects of leucovorin (LV) on 5-fluorouracil (5-FU) and the inhibition rate of thymidylate synthase (TS) activity in Colon 26 murine colon carcinoma cells and L1210 murine leukemia cells. Methods In cytotoxic and TS inhibition studies, cells were exposed for 24 hours to varied concentrations of 5-FU alone or in combination with 1 or 10 μmol/L LV. Cytotoxicity was determined by colony forming efficiency or trypan blue dye exclusion, and TS inhibition rate was determined by [6-³H]-5-FdUMP binding assay. A growth inhibition curve was constructed for longer exposures. Results For tumor cell growth inhibition and cytotoxicity, the augmentative effect was observed when the lower 5-FU concentrations (0.1 μg/mL) were used. There was no difference in the effects between the low (1 μmol/L) and high (10 μmol/L) LV doses. Normally, TS enzyme levels rise acutely when cells are exposed to 5-FU. Both cell lines displayed an increase in TS after exposure to 5-FU. This exposure to 5-FU resulted in the maintenance of free enzyme. LV was able to increase the ternary complex and TS inhibition rate with little induction of TS, however, the inhibition rate was not dependent on doses of LV. Conclusions It was concluded that the augmentative effect of LV at a concentration of 0.1 μg/mL 5-FU occurred at the clinically achievable levels of 1 μmol/L of LV, and there was no difference in the effect between the low and high doses. TS was inhibited effectively by 5-FU and the addition of LV, without a marked induction of TS.     

手术径路对贲门癌远期生存的影响(附353例随访结果分析)

了解手术径路对贲门癌术后远期生存率的影响。方法：对本院353例贲门癌分左胸、上腹及胸腹联合切口三种径路进行手术，术后逐年随访，了解手术径路对贲门癌术后远期生存的影响。结果：全组总1、3、5、10年生存率分别为86．1％、56．7％、49．9％、20．9％。左胸组、上腹组及胸腹联合切口组5年生存率分别为37．6％，46．25％，57．6％。三组间各TNM分期的5年生存率经用加权卡方检验差异并无显著性（P＞0．05）。而肿瘤的浸润深度与淋巴结的转移状况对其预后有显著影响（P＜0．01）。     

Modulation of 5-fluorouracil with methotrexate and low-dose N-(phosphon-acetyl)-L-aspartate (PALA) is inactive in advanced pancreatic carcinoma

Purpose: To evaluate the effect of biochemical modulation by PALA and methotrexate on the therapeutic activity of 5-fluorouracil (5-FU) in patients with advanced pancreatic adenocarcinoma.Patients and methods: The treatment protocol consisted of phosphonacetyl-L-aspartate (PALA) 250 mg/m² i.v. 15-minute infusion followed by methotrexate 200 mg/m² i.v. 30-minute infusion on day 1 and 5-FU 600 mg/m² i.v. push on day 2. Folinic acid was given at 15 mg/m² p.o. every six hours for eight doses, starting 24 hours after methotrexate infusion. Cycles were repeated every two weeks.Results: Thirty patients with advanced chemotherapy-naive pancreatic cancer were included; 26 had measurable disease. Median age 56 years (27–72); median PS 1 (0–2). One PR (3.9%) was achieved; nine patients had stable disease. Median time to progression was 91 days. Median survival was 177 days and one year survival was 13.3% (4 of 30 patients). Treatment was well tolerated; diarrhea WHO grade 2 or 3 occurred in six patients; stomatitis WHO grade 2 and 3 in nine patients.Conclusions: Modulation of 5-FU by PALA and MTX given in this dose and schedule appears to be ineffective in patients with advanced pancreatic adenocarcinoma.