使用先锋铋后饮酒中毒5例报告

先锋铋系第三代头孢菌素，对革兰氏阳性菌及革兰氏阴性菌均有较强抗菌活性。在基层医院它是一种常用抗生素，如使用后短期内（〈12h）饮酒易致中毒，现报告如下。     

不同微生物诱导家蝇幼虫表达抗菌肽的特性

研究不同微生物诱导家蝇幼虫表达的抗菌肽特性.用3种不同的病源菌通过针刺感染的方法诱导家蝇幼虫表达抗菌肽,通过Sephadex G25分离,用Hult mark改进法和抑菌圈测定法作抑菌试验,用毛细管电泳（CE）分析不同微生物诱导得到的抗菌肽样品差异,检测抗菌肽的热稳定性和酸碱耐受性.发现不同微生物诱导产生的家蝇抗菌肽具有广谱抑菌性,但不同样品对不同病源菌抑菌活性有差异,不同测定抑菌效果的方法对抑菌结果有影响,各种抗菌肽样品CE蛋白谱具有明显不同.抗菌肽样品都具有热稳定性和酸碱耐受性.说明不同微生物诱导产生的家蝇抗菌肽类型以及抗菌肽含量与诱导源有关,抗菌肽为家蝇幼虫体内固有成分,诱导增加了抗菌肽的表达量同时刺激新抗菌肽的产生.用志贺氏菌、金黄色葡萄球菌、鼠伤寒沙门氏菌诱导家蝇幼虫可产生较多含量高活性好的抗菌肽.     

从单纯高血压患者体内分离出的外周单核细胞中组织因子表达和活性并不升高

背景:系统性高血压是动脉粥样硬化血栓形成的主要危险因素之一。研究发现组织因子(TF)位于血管外膜和粥样斑块的脂质核心,并特异地表达于单核细胞和巨噬细胞膜表面。TF在凝血生理中起到重要作用,同时也参与促炎症过程和粥样硬化斑块失稳定。目的:在本研究中,对从具有动脉粥样硬     

NK／LAK细胞对人口腔癌耐细胞药株杀伤活性的观察

OBJECTIVE: To understand the relation between cytotoxic activity of immunologic effector cells and multidrug resistance of the tumor cells. METHODS: Continuous observation of the morphological changes and MTT colorimetry were employed to evaluate the cytotoxic activity of lymphokine-activated killer (LAK) cells and natural killer (NK) cells against multidrug-resistant (MDR) human oral carcinoma cell line-KBV200 (before and after reversal of MDR) and parental drug-sensitive cell line KB. The morphologic changes of LAK cells and the 3 target cell lines were observed continuously under inverted microscope 3 h after co-culture of LAK cells with one of three target cell lines respectively. The lysis rates of three tumor cell lines in response to co-culture with LAK or NK cells were determined using MTT colorimetry. RESULTS: In comparison with the parental drug-sensitive cell line KB, both KBV200 and its reserved cell line by verapamil (KBVV) showed earlier adherence and greater number of cells lysed by LAK. In MTT colorimetry assay, the cytotoxicity of both LAK and NK cells against the 3 cell lines was associated with the effector-to-target (E/T) cell ratio; the lysis rates of KBV200 and reversed KBV200 cells by verapamil in response to LAK and NK cells were higher than that of KB cells (P<0.05), but KBV200 and KBVV did not significantly differ (P>0.05). At the same E/T ratio, LAK cells possessed stronger cytotoxicity than NK cells against all the tumor cell lines (P<0.05). CONCLUSIONS: Immunologic effector cells possess strong cytotoxic activity against multidrug-resistant cell line KBV200. Modulation of MDR does not decrease the cytotoxic activity of the immunologic effector cells. The results of this study suggest that adoptive cell immunotherapy with immunologic effector cells may be of value in controlling the progress of MDR tumors.     

高血压病的几种相关因素

高血压病是我国最常见的心血管疾病，其发病机制非常复杂。近年来，大量的试验研究发现，某些血管活性物质、高胰岛素血症、脂酯代谢异常以及不良习惯、心理因素等，在高血压病的发生发展过程中起着促进与协同作用。     

甲型肝炎

甲型肝炎病毒PlB、P2A、P3AB和P3D蛋白在原核系统中的表达和抗原活性分析——宁尚勇等（北京中国疾病预防控制中心病毒病预防控制所肝炎室100052）；《中华实验和临床病毒学杂志》，2307，21（1）：50-52[目的：研究甲型肝炎病毒P1B、P2A、P3AB和P3D在原核系统中的表达，并探讨这些蛋白的抗原活性和作为诊断抗原的应用价值。     

现代医学200词 199．白三烯受体

白三烯（1eukotriene，LT）是由花生四烯酸在5-脂肪氧合酶的作用下由生成的LTA。构成的生理活性脂类，可以分为LTB4和半胱氨酰LT（CysLTs：LTC4，LTD4，LTE4）。     

清洁验证中化学残留物检测分析方法验证

本文以苯巴比妥作为残留物为例说明清洁验证中残留物检测分析方法的验证。