温针配合中西药治疗膝关节滑膜炎68例

膝关节滑膜炎是临床常见病之一,多因急性损伤或慢性劳损等因素引起,并因肿胀而影响患者的活动功能,行走不便。过去治疗本病多采用穿刺、输液、针灸、理疗等方法,但治疗效果多不持久,易反复发生。笔者运用温针配合中西药外用治疗膝关节滑膜炎68例,取得了较为满意的疗效,现报告如下。     

温针灸治愈中风偏瘫之肩痛1例

中风偏瘫初期的功能锻炼若未重视患侧肩关节的保护及锻炼,就可出现该侧肩关节较剧烈的疼痛、甚至脱位,阻碍进一步的功能锻炼。此种情况在临床常常碰到,但单纯的给予保护     

非程序性冷冻仪冻存人胚胎干细胞的方法

在人胚胎干细胞（human embryonic stem，hES）的研究中，有效的冻存方法非常重要。目前，冷冻Es细胞主要有两种方法：慢速冷冻一快速复温和超快速玻璃化法。慢冻法需要昂贵的程序性冷冻仪，而玻璃化法步骤繁琐且只能冻存小量细胞．不能满足临床应用。因此，本研究旨在寻找非程序性冷冻仪冻存大量hES细胞的方法。     

兔肢体爆炸伤合并海水浸泡后在不同复温速率及维持浅低体温下机体炎症反应的特征

Objective To investigate effects of different rewarming rates and maintenance of light hypothermia on inflammatory response in rabbits after limb blast injury, coupled with seawater immersion. Methods First, the model of limb blast injury coupled with seawater immersion was reproduced [the animals were immersed to low body temperature of (31.0±0.5℃)]. Then, 24 adult rabbits were randomly divided into group Ⅰ [the rapid rewarming group, n=6, rewarmed to (38±0.5)℃ at a rate of (8.94±0.93)℃/h], group Ⅱ [the slow rewarming group, n=6, rewarmed to (38±0.5)℃ at a rate of (3.88±0.22)℃/h], group Ⅲ [another slow rewarming group, n=6, rewarmed to (38±0.5)℃ at a rate of (2.18±0.12)℃/h], and the H group [the hypothermia group, n =6, rewarmed to (34 - 35)℃ at a rate of (4.49±0.66)℃/h and kept at that temperature till termination of the experiment]. Regulation of ambient temperature and warm transfusion were used to restore body temperature to target levels and maintained there for 6 hours. Blood samples were taken at 5 different times, I.e. Pre-injury time(T0), post-immersion time (T1), the time when rewarming started (T2), 3 h after rewarming (T3), and 6 h after rewarming (T4). Tissue samples from heart, liver, intestinum, lung and kidney were also collected. Levels of TNF-α (tumor necrosis factor-α), IL-1β (interleukin-1β) and IL-6 (interleukin-6) in plasma and MPO (myeloperoxidase) in homogenate were detected. Results Following rewarming, TNF-α, IL-1β, IL-6 concentrations in the plasma of the animals in group Ⅰ and group H were significantly higher when compared with those of the animals in group Ⅱ and group Ⅲ (P<0.05, P<0.01), and MPO activity in homogenate was significantly higher when compared with that of the animals in group Ⅱ and group Ⅲ(P<0.01, P<0.05), and no statistical difference could be seen between group Ⅱ and Ⅲ (P>0.05). Conclusions Rapid rewarming and maintenance of light hypothermia could obviously elevate TNF-α, IL-1β, IL-6 concentrations in plasma and MPO activity in homogenate, following limb blast injury coupled with hypothermia induced by seawater immersion, while slow rewarming (with a rewarming rate of 2-4℃/h) could significantly inhibit TNF-α, IL-1β, IL-6 levels and PMN activity.     

小儿肠套叠行温生理盐水灌肠复位的护理

小儿肠套叠是婴幼儿常见的急腹症之一，多见于2岁以内肥胖健壮的婴幼儿，以4～10个月龄最多见。典型临床表现为突然发病，阵发性哭吵、腹痛、呕吐、血便(黏液果酱样大便)，腹部肿块。以往对小儿肠套叠非手术治疗均采用X线下行空气灌肠复位治疗。2001年2月-2004年2月，我院开展实施超声监视下行温生理盐水灌肠复位治疗该类患儿60例，取得了良好效果，现将临床护理情况报道如下。     

温针灸为主治疗肩周炎50例疗效观察

目的 观察温针灸、推拿配合功能锻炼治疗肩周炎的临床效果。方法 运用温针灸、推拿配合功能锻炼治疗肩周炎患者50例并同针刺组48例比较。结果 观察组总有效率为98．0％，对照组总有效率为83.33％，观察组疗效明显优于对照组。结论 温针灸、推拿配合功能锻炼治疗肩周炎有良好的疗效。     

几种手性药物对映体的HPLC分离与生物样品分析

使用ＣＨＩＲＡＬ－ＡＧＰ柱，通过优化色谱条件，以ＨＰＬＣ法对３种手性药物ＳＦＺ－４７〔（±）－３Ｈ－１，２－二氢－２－（４－甲基苯胺基）－甲基－１－吡咯里嗪酮〕，ＫＭＢＺ－００９〔（±）－反式－４－苯基－５－（邻氯苄基）－吡咯烷酮－２〕和地丙苯酮〔（±）－２′－（２－羟基－３－（特戊胺基）丙氧基）－３－苯基苯丙基酮〕进行了对映体的直接分离，达到良好的分离效果．在此基础上测试了动物血浆样品中ＳＦＺ－４７的对映体，对其药物动力学的对映体选择性进行了初步研究．     

柱凝集血型检测技术的临床应用

自1988年柱凝集法血型检测技术问世以来,以其操作简单,灵敏度高、特异性强,受到输血界同仁重视。我们使用柱凝集技术进行血型检测3600例,其中博唯优玻璃珠柱400例,达亚美凝胶3200例。准确率在98.6％以上,改变了过去的传统玻片法,为临床安全输血奠定了基础。1 材料与方法1.1 试剂 达亚美血型卡(凝胶法),博唯优血型卡(玻璃珠     

温缺血时间与同种心脏瓣膜细胞活性的关系

以１０具脑外伤死亡的健康男性的心脏的６０个同种主动脉、肺动脉瓣叶分为６组进行实验。经不同的温缺血时间灭菌并经过３个月的液氮深低温保存后，通过流式细胞计数测定细胞活性，同时行组织块贴壁培养及电镜观察。结果表明：温缺血时间在１２小时以内，细胞活性为９３．４９±２．３５％，较对照组无明显下降。灭菌２４小时，细胞活性为８９．８２±３．５２％，较对照组明显下降，Ｐ＜０．０１，并且可见形态上改变；冷冻后细胞活性进一步下降，但下降幅度减低，冻存３年后活细胞仍占８０％。结论为：温缺血时间对细胞活性有影响，时间延长与活性的下降成正相关。因此温缺血时间应控制在１２小时以内。