核因子-κΒ与EGF在脑膜瘤组织中表达的相关性研究

目的探讨核因子-κΒ(nuclear factor kappa binding,NF-κB)与表皮生长因子(epidermal growth factor,EGF)在脑膜瘤组织中表达的相关性.方法采用免疫组化SP法测定50例脑膜瘤组织、10例正常硬脑膜组织中NF-κΒ p65、EGF、表皮生长因子受体(epidermal growth factor receptor,EGFR)的表达水平.结果 NF-κB р65、EGF、EGFR在脑膜瘤组织中和正常硬脑膜组织中的表达差异均存在显著性(P＜0.005),NF-κΒ p65与EGF表达具有相关性(r＝0.4524,P＝0.001＜0.005),与EGFR的表达无相关性(r＝-0.2506,P＝0.079＞0.05).结论 NF-κB与EGF在脑膜瘤组织中的表达呈正相关,提示NF-κB可直接调控EGF基因转录,从而促进脑膜瘤细胞的增殖.     

重组2型腺相关病毒-转化生长因子β3对实验性肝纤维化大鼠肝脏组织病理学和Ⅰ型胶原表达的影响

Objective To investgate the effects of TGF β 3 on rat hepatic fibrosis. Methods The TGF β 3 cDNA was cloned into rAAV2 vector. Rats were randomly divided into four groups: normal control group, model group, negative control group and TGF β 3 group. Hepatic fibrosis was induced by hypodermic injection of 40% CCI4. Recombinant AAV2-TGF β 3 viral particles were injected via the vena caudalis one week before CCh treatment. Rats were executed 8 weeks after CCI4 treatment, global histological change was observed after HE staining, the distribution of the collagen fibers was observed after masson staining, his-tochemistry was done to observe the expression of collagen Ⅰ; The positive area rate of the collagen fibers and the average optical rate of collagen Ⅰ were quantified. Results HE staining indicated that collagen fibers were reduced in the TGF β 3 group. Masson staining shown that the collagen fibers were distributed around the blood vessel, in the portal area and disse space. Compared to the model group (13.2%±2.2%) and negative control group (12.3%±1.5%), the collagen fibers in liver tissues of TGF β3 group (7.7% ± 1.5%) were significantly decreased (q = 9.456, P < 0.01; q = 8.217, P < 0.01). Histochemistry indicated that the collagen fibers of liver tissues of TGF 15 3 group (0.185±0.033) were significantly higher than those in the model group (0.252±0.042) and the negative control group (0.230±0.029), (q = 6.228, P < 0.01; q = 4.346, P < 0.01). Conclusion TGF β 3 alleviates the damage to hepatic cell and the level offibrosis in CCI4 treated rats and inhibits the expression of collagen Ⅰ.     

环形电刀切除术治疗宫颈病变的近期观察

由于人民对自身健康的重视以及电子阴道镜在临床上的广泛应用,宫颈上皮内瘤样病变（cIN）早期发现逐渐增加.近年来,随着对高频电波刀的性能的进一步了解,宫颈电圈切除（the loope lectrosurgical excision procedure,LEEP）在治疗宫颈病变的应用上日益普遍.现将我院从2004年3月至2005年2月对128例各种宫颈病变试用LEEP治疗情况报告如下.     

Effect of Short—Term Exposure to Mercuric Chloride on the Air—Breathing Catfish,Heteropneustes fossilis

Alterations in the respiratory epithelium of gills of a catfish,Heteropneustes fossilis by the sublethal and lethal concentrations of HgCl2 have been observed using scanning electron microscope,Fish exposed to 0.1ppm shows a thin coat of mucus,but the continuous layer of mucus is not retained in 0.3ppm after 24 h of exposure.Epithelial cells of the gill filament are hyperplastic.Other changes in the respiratory epithelium are contraction of epithelium,formation of interlamellar bridge and diminishing number of microridges.Massive destruction of the respiratory epithelium by deep lesions and sloughing of epithelial layer in 0.5 and 1.0ppm solutions after 6-12 h of exposure apparently causes death to the fish.SEM observations are well in conformity wit the findings made at light microscopic level.     

VEGF-D在乳腺癌中的表达及作用

目的探讨VEGF-D在乳腺癌淋巴结转移中的作用。分析乳腺癌组织内部淋巴管生长因子的不同表达与腋窝淋巴结转移之间的关系。方法采用免疫组化Envision二步法对80例人类乳腺浸润性导管癌组织的石蜡切片分别进行检测,观察VEGF-D的表达状态。结果80例乳腺癌组织中62例VEGF-D呈阳性表达,阳性率为77.5%（62/80）;其中有淋巴结转移的阳性率78.8%（41/52）。结论乳腺癌细胞中存在VEGF-D,其表达状态与乳腺癌淋巴结转移关系密切。VEGF-D可能是原发灶肿瘤细胞和周围淋巴管内皮细胞之间相互旁分泌关系的重要调节因素,在淋巴管转移的发生中可能起重要的作用。     

胰岛素样生长因子家族与糖尿病肾病

胰岛素样生长因子家族包含多个成员 ,在糖尿病肾病中存在异常表达 ,IGFs家族的组成、生理特性及在糖尿病肾病发病机制中的作用日益受到关注。     

子宫颈上皮内瘤变及子宫颈鳞癌中KGF、p16表达及作用

<正>子宫颈鳞癌是常见的妇科恶性肿瘤之一,发病率在女性恶性肿瘤中居第2位。随着子宫颈上皮从单纯增生→不典型增生→原位癌→浸润癌的进展,癌变的危险依次升高。而上皮细胞的不典型增生,被认为是     

卵巢浆液性癌中VEGF的表达与有丝分裂活性及FIGO分期相关

为探讨腹腔镜行双侧盆腔和主动脉旁淋巴结切除术在肥胖患者中的应用价值,对Oklahoma大学1996年1月8日～2001年1月14日期间诊断为子宫内膜癌且肥胖指数(QI)≥28的患者进行回顾性研究。腹腔镜行双侧盆腔、主动脉旁淋巴结切除者为1组(55例);开腹行淋巴结切除者为2     

平阳霉素治疗上皮样血管瘤1例

1临床资料患者女，48岁，因左颞部出现多个暗红色丘疹，结节，近2年，反复治疗无效，诊断不明，于2003年3月就诊，患者自述，2000年10月左颞部起一绿豆大丘疹，无自觉症状，丘疹渐多并出现多个大小不等的皮下结节。于2001年5月在外院行手术切除，术中出血较多，术后一周便复发，再次彻底切除，