全文获取类型
收费全文 | 339篇 |
免费 | 45篇 |
国内免费 | 13篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 13篇 |
妇产科学 | 1篇 |
基础医学 | 24篇 |
口腔科学 | 6篇 |
临床医学 | 21篇 |
内科学 | 32篇 |
皮肤病学 | 23篇 |
神经病学 | 31篇 |
特种医学 | 5篇 |
外科学 | 17篇 |
综合类 | 47篇 |
预防医学 | 25篇 |
眼科学 | 5篇 |
药学 | 95篇 |
中国医学 | 36篇 |
肿瘤学 | 14篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 10篇 |
2020年 | 10篇 |
2019年 | 13篇 |
2018年 | 7篇 |
2017年 | 14篇 |
2016年 | 19篇 |
2015年 | 16篇 |
2014年 | 27篇 |
2013年 | 26篇 |
2012年 | 19篇 |
2011年 | 31篇 |
2010年 | 20篇 |
2009年 | 15篇 |
2008年 | 10篇 |
2007年 | 13篇 |
2006年 | 11篇 |
2005年 | 7篇 |
2004年 | 13篇 |
2003年 | 19篇 |
2002年 | 9篇 |
2001年 | 17篇 |
2000年 | 4篇 |
1999年 | 11篇 |
1998年 | 7篇 |
1997年 | 11篇 |
1996年 | 3篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1992年 | 4篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 3篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1982年 | 3篇 |
1981年 | 1篇 |
1980年 | 1篇 |
1978年 | 2篇 |
1975年 | 2篇 |
排序方式: 共有397条查询结果,搜索用时 0 毫秒
1.
应用细胞原位杂交技术,观察经重组小鼠白细胞介素-19(IL-1β)处理后的体外培养的新生1d大鼠中脑黑质神经元c-jun基因的表达.结果显示,培养的黑质细胞多为酪氨酸羟化酶阳性神经元,IL-1β可诱导体外培养的黑质神经元c-junmRNA表达,高水平的表达出现在IL-1β处理后2~4h。说明IL-1β有兴奋黑质神经元的作用,并提示黑质神经元上可能存在IL-1β受体. 相似文献
2.
Frank P. Castronovo Jr. Ph.D. H. William Strauss M.D. Kenneth A. McKusick M.D. Majic S. Potsaid M.D. 《Skeletal radiology》1982,7(4):233-237
A skeletal seeking radiopharmaceutical labeled with a long-lived radionuclide was developed to evaluate regional bone formation and its subsequent resorption. The agent is [phosphonate (phenylmethylene hydroxy) bis]-I-125 or I-125 PA. Tissue distribution studies in mice (N=16) showed approximately 40% of the administered dose to be retained by the skeleton up to 336 hours post IV injection. The percentage of the dose accumulated by the thyroid gland remained at less than 0.5%, indicating minimal deiodination of the I-125 PA. Whole body retention studies in the same species revealed a triexponential release pattern with the longest component comprising 33% of the dose with a biologic half-life of 962 days. A fractured rat tibia model was studied with I-125 PA and Tc-99m MDP. Chronic loss of the I-125 PA relative to normal tibia was quantitated: five days (62.8%); 30 days (47.4%). Concomitant increased uptake of the Tc-99m MDP was observed at the fracture site relative to normal: five days (186%); 30 days (1,041%). The above data suggest that I-125 PA can be utilized to measure acute bone formation and chronic resorption. 相似文献
3.
目的:观察艾司西酞普兰治疗抑郁症的疗效及安全性。方法:40例住院的抑郁症患者随机分为艾司西酞普兰组和氟西汀组,疗程为6周,以Hamilton抑郁量表(HAMD)评定疗效,TESS评定不良反应。结果:艾司西酞普兰疗效优于氟西汀(P〈0.05),且不良反应较少(P〈0.05)。结论:艾司西酞普兰治疗抑郁症疗效确切且安全性高,值得临床上进一步推广。 相似文献
4.
Identification of a major metabolite of danshensu in rat urine and simultaneous determination of danshensu and its metabolite in plasma: application to a pharmacokinetic study in rats 下载免费PDF全文
Xiangyang Wang Wei Li Xiaohui Ma Kaijing Yan Yang Chu Min Han Shuming Li Hongchao Zhang Shuiping Zhou Yonghong Zhu He Sun Changxiao Liu 《Drug testing and analysis》2015,7(8):727-736
Danshensu, as the effective component of Salvia miltiorrhiza (Danshen), has been widely used in clinical studies for treatment of cardiovascular diseases in China. A new metabolite, 4‐hydroxy‐3‐methoxyphenyllactic acid was isolated from the urine of rats, and its chemical structure was identified by ultraviolet (UV), Infrared Spectroscopy (IR), mass spectrometry (MS) and nuclear magnetic resonance (NMR). Furthermore, a selective and sensitive high performance liquid chromatography‐tandem mass spectrometric (HPLC‐MS/MS) method was developed for the simultaneous quantification of danshensu and its major metabolite, 4‐hydroxy‐3‐methoxyphenyllactic acid, in rat plasma after oral and intravenous administration of danshensu. The separation was performed on a Hypersil Gold C18 column (150 × 2.1 mm i.d., 3.0 µm, Thermo, San jose CA, USA) with gradient elution using a mobile phase composed of methanol and water (containing 0.1% formic acid) at a flow rate of 0.2 mL/min. Linear detection responses were obtained for danshensu and 4‐hydroxy‐3‐methoxyphenyllactic acid ranging from 5 to 10000 ng/mL and 5 to 4000 ng/mL, respectively. The lower limits of quantification (LLOQs) for the two compounds were both 5 ng/mL. The intra‐and inter‐day precision (R.S.D %) were within 5.61% for the two analytes. The average recoveries of the analytes were greater than 72.43%. The method was proved to be stable during all sample storage, preparation and analytic procedures. This method was successfully applied to the pharmacokinetic studies of danshensu and 4‐hydroxy‐3‐methoxyphenyllactic acid after oral and intravenous administration of danshensu in rats.Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
5.
Souberbielle JC Prié D Courbebaisse M Friedlander G Houillier P Maruani G Cavalier E Cormier C 《Annales d'endocrinologie》2008,69(6):501-510
Knowledge about vitamin D has greatly improved during the last few years. Vitamin D cannot any more be considered as exclusively necessary to prevent ricket/osteomalacia. Its role in the prevention of some osteoporotic fractures in the elderly (in association with calcium nutrition) is now well demonstrated and many epidemiologic and laboratory data argue for a role in the prevention of several diseases or anomalies (cancer, auto-immune diseases, cardiovascular events, sarcopenia...). A few intervention studies confirming some of these effects also exist. Vitamin D status can easily be assessed by measuring serum 25 hydroxy vitamin D (25OHD) level. However, many experts have claimed that the population-based reference values for 25OHD are too low and that the cut-off value below which vitamin D insufficiency can be present is somewhere between 20 and 40 ng/mL with a clear tendency to target values above 30 ng/mL (75 nmol/L). The main consequences are that vitamin D insufficiency is highly frequent whereas the currently recommended supplementation doses are not sufficient. 相似文献
6.
Correlations between Serum P2X7, Vitamin A, 25‐hydroxy Vitamin D,and Mycoplasma Pneumoniae Pneumonia
Fanjun Meng Ping Chen Xiaolong Guo Xiaoru Li Yuexuan Wu Wenen Liu Feng Jiang Huan Liu Lixin Wang 《Journal of clinical laboratory analysis》2021,35(5)
BackgroundIdentifying new molecular diagnostic markers for Mycoplasma Pneumoniae Pneumonia (MPP) has always been an essential topic since MPP cases have increased every year, especially among children. Here, we examined the correlation between serum level of Purinergic receptor P2X7, vitamin A, and 25‐hydroxy vitamin D (25(OH)D) and the severity of MPP, aiming to identify molecules that have the potential to become diagnostic markers.MethodsThis study was conducted on 186 cases aged 1–14 (136 MPP and 50 non‐MPP patients). Serum levels of Purinergic receptor P2X7, vitamin A, 25(OH)D, and multiple inflammatory and immune factors were measured, compared, and tested for statistical significance.ResultsSerum P2X7, tumor necrosis factor‐α (TNF‐α), and interleukin‐1β (IL‐1β) levels were significantly increased in severe MPP patients, while serum vitamin A, 25(OH)D, IgA, and IgG levels were significantly decreased.ConclusionOur results demonstrated a positive correlation between serum P2X7 level and the severity of MPP, and negative correlations between serum levels of vitamin A and 25(OH)D and the severity of MPP, suggesting that high serum levels of P2X7 and low serum levels of vitamin A and 25(OH)D may indicate relatively severer MPP. 相似文献
7.
《Journal of biomaterials science. Polymer edition》2013,24(5):381-395
in the first part of this article calorimetric studies on poly (hydroxy ethyl methacrylate) (PHEMA) are presented. In the past the irregular melting curves in this type of experiment have been interpreted as evidence for the existence of different types of water in these gels. The studies presented here demonstrate that the occurrence of a glass transition in the freezing hydrogels may be responsible for this irregular melting behavior, and that this behavior is not (necessarily) an indication for the existence of different types of water. In the second part results are shown of measurements of the mobility of water in hydrogels, made by relaxation NMR. These results indicate that very rapid interchange occurs between the water molecules, and they support the conclusion that the calorimetric data mentioned above are not indicative for the existence of different classes of water in hydrogels. These results are compared with data from other fields of science, especially from fundamental freeze drying studies, which support the alternative interpretation of the calorimetric measurements. 相似文献
8.
Gregory L. Krauss Emilio Perucca Elinor Ben‐Menachem Patrick Kwan Jerry J. Shih David Squillacote Haichen Yang Michelle Gee Jin Zhu Antonio Laurenza 《Epilepsia》2013,54(1):126-134
Purpose: To evaluate safety, tolerability, and seizure outcome data during long‐term treatment with once‐daily adjunctive perampanel (up to 12 mg/day) in patients with refractory partial‐onset seizures. Methods: Study 307 was an extension study for patients completing the double‐blind phase of three pivotal phase III trials (studies 304, 305, and 306). The study consisted of two phases: an open‐label treatment phase (including a 16‐week blinded conversion period and a planned 256‐week maintenance period) and a 4‐week follow‐up phase. Patients were blindly titrated during the conversion period to their individual maximum tolerated dose (maximum 12 mg/day). Adverse events (AEs) were monitored throughout the study and seizure frequency recorded. The interim data cutoff date for analyses was December 1, 2010. Key Findings: In total, 1,218 patients were enrolled in the study. At the interim cutoff date, 1,186 patients were in the safety analysis set; 1,089 (91.8%) patients had >16 weeks of exposure to perampanel, 580 (48.9%) patients had >1 year of exposure, and 19 (1.6%) patients had >2 years of exposure. At the interim analysis, 840 (70.8%) patients remained on perampanel treatment. The large majority of patients (n = 1,084 [91%]) were titrated to 10 mg or 12 mg/day. Median (range) duration of exposure was 51.4 (1.1–128.1) weeks. Treatment‐emergent AEs were reported in 87.4% of patients. The most frequent were dizziness (43.9%), somnolence (20.2%), headache (16.7%), and fatigue (12.1%). Serious AEs were reported in 13.2% of patients. In the intent‐to‐treat analysis set (n = 1,207), the frequency of all seizures decreased over the first 26 weeks of perampanel treatment in patients with at least 26 weeks of exposure to perampanel (n = 1,006 [83.3%]); this reduction was maintained in patients with at least 1 year of exposure (n = 588 [48.7%]). The overall median percent changes in seizure frequency in patients included in each 13‐week interval of perampanel treatment were ?39.2% for weeks 14–26 (n = 1,114), ?46.5% for weeks 40–52 (n = 731), and ?58.1% for weeks 92–104 (n = 59). Overall responder rates in patients included in each 13‐week interval of perampanel treatment were 41.4% for weeks 14–26 (n = 1,114), 46.9% for weeks 40–52 (n = 731), and 62.7% for weeks 92–104 (n = 59). During the blinded conversion period, the reduction in seizure frequency in patients previously randomized to placebo (?42.4%, n = 369) was similar to that in patients previously randomized to perampanel (?41.5%, n = 817). Significance: Consistent with pivotal phase III trials, these interim results demonstrated that perampanel had a favorable tolerability profile in patients with refractory partial‐onset seizures over the longer term. The decrease in seizure frequency was consistent and maintained in those patients over at least 1 year of perampanel exposure. 相似文献
9.
支气管哮喘患儿血清25-羟维生素D3水平及其与肺功能、呼出气一氧化氮的关系 总被引:1,自引:0,他引:1
目的:了解支气管哮喘(简称哮喘)患儿血清25-羟维生素D3[25(OH)D3]水平及其与肺功能、呼出气一氧化氮( FeNO)的关系,为哮喘的治疗提供新的策略。方法选取门诊6~14岁初诊哮喘患儿112例为观察组,选取同期在儿童保健科体检的6~14岁健康儿童78例为对照组,通过电化学发光法检测两组患儿血清中25( OH)D3的含量,检测哮喘患儿呼气峰流速( PEF)、第1秒用力呼气容积( FEV1)、呼气峰流速占预计值百分比( PEF% pred )、第1秒用力呼气容积占预计值百分比( FEV1% pred),对观察组患儿进行FeNO检测。对25( OH)D3与PEF% pred、FEV1% pred、FeNO的相关性进行分析。结果观察组的维生素 D 缺乏、不足比例均高于对照组(χ2=7.78,P ﹤0.01);PEF% pred、FEV1% pred的值随血清25(OH)D3水平的下降而降低(F=28.12、29.56,P均﹤0.05), FeNO值随血清25(OH)D3水平的下降反而升高(F=15.65,P﹤0.05)。结论维生素D缺乏或不足与儿童哮喘相关,与哮喘患儿肺功能下降有关,且可增加哮喘患儿气道炎症水平。 相似文献
10.
Yuan Tian Yang Hong Samuel J. Bonacorsi Aaron Balog Sharon Gong 《Journal of labelled compounds & radiopharmaceuticals》2014,57(1):1-11
For the first time, [3α‐3H] 17α‐hydroxy pregnenolone (1) was synthesized through a multiple step sequence. The presence of [3β‐3H] isomer in RP‐HPLC purified product was identified by tritium NMR. The [3β‐3H] isomer was then separated from [3α‐3H] 17α‐hydroxy pregnenolone with chiralPAK AD‐H column. [3α‐3H] pregnenolone (2) was synthesized from commercial available 5‐pregnen‐3,20‐dione in one step with an improved procedure. 相似文献