针刺治疗慢性荨麻疹50例及其对血清补体C3的影响

目的 :探讨有效提高慢性荨麻疹疗效的方法及其免疫机理。方法 :92例慢性荨麻疹患者随机分为观察组 50例和对照组 42例 ,观察组采用针刺合谷、血海、足三里、三阴交穴联合抗组胺药仙特敏治疗 ,对照组单纯使用仙特敏 ,观察疗效及治疗前后血清补体C3 的变化。结果 :观察组有效率92 0 % ,对照组有效率 73 6 % ,差异有显著性意义 (P <0 0 5) ;慢性荨麻疹患者血清补体C3 治疗前平均水平较健康成人低 ;观察组补体C3 水平治疗前后差异有显著性意义 (P <0 0 5)。结论 :针刺联合抗组胺药仙特敏疗效优于单纯仙特敏 ,且能相应提高患者的补体C3 水平     

《内经》和《中藏经》中言语与语言异常的比较

<内经>和<中藏经>中对言语和语言异常的记载散见于各篇.在言语和语言异常的描述方式上,<内经>较<中藏经>多样化.在临床价值上,言语和语言异常在<中藏经>中较少被用于指导临床诊断治疗,而在<内经>中应用较多,如用于阐明体质人格和临床误治等.     

邻苯二甲酸二丁酯诱导尿道下裂大鼠模型的建立及其作用机制

目的用邻苯二甲酸二丁酯(DBP)诱导建立尿道下裂大鼠模型并在分子水平研究其导致尿道下裂的作用机制。方法将孕鼠在怀孕第12～19天用DBP(500mg/kg体重)连续每天灌胃建立仔代雄鼠尿道下裂模型，并将孕鼠在第19天行破宫产取仔代雄鼠的睾丸组织，用逆转录一聚合酶链反应(RT-PCR)的方法检测睾酮合成过程中限速酶细胞色素P450胆固醇侧链裂解酶(P450scc)、3p羟基类固醇脱氢酶(3p-HSD)和细胞色素P45017α-羟化酶(P450c17)的mRNA含量水平，与正常对照组进行比较。结果DBP诱导的尿道下裂组胎鼠睾丸组织中P450scc、3β-HSD和P450c17的基因表达水平较正常对照组均有不同程度的下降，两组间差异比较均有统计学意义(P＜0．05)。结论DBP通过在性发育期干扰仔代雄鼠睾丸组织中睾酮的生物合成途径，降低了睾酮水平，导致大鼠出生后尿道下裂的出现。     

青地汤防治肺癌放疗反应效果观察

①目的　探讨青地汤防治肺癌放疗反应的效果。②方法　 1 56例肺癌病人分为两组 ,青地汤组 84例连续服用青地汤 2 0剂以上 ,对照组 72例未服药。比较两组放疗反应的发生率及 1、2年生存率。③结果　青地汤组放射性气管炎、放射性肺炎、放射性肺纤维化及因严重放疗反应而被迫中断放疗的发生率分别为 36 .9%、7.1 %、2 5 .0 %、1 0 .7% ,对照组分别为 54 .2 %、1 9.4%、45 .8%、2 7.8% ,两组比较差异有显著性 (χ2 =4.670～ 7.459,P均 <0 .0 5)。青地汤组放射性食管炎发生率 ,治疗中抗生素及糖皮质激素的应用率 ,病人的 1、2年生存率分别为57.1 %、41 .7%、2 6 .2 %、37.8%、2 6 .5 % ,对照组分别为 65 .3 %、54 .2 %、40 .3 %、35 .5 %、2 0 .7% ,两组比较差异无显著性 (χ2 =0 .0 65～ 3 .496 ,P均 >0 .0 5)。④结论　青地汤能有效防治肺癌病人的放疗反应     

脑源性神经营养因子及神经干细胞移植治疗新生大鼠缺氧缺血性脑损伤的实验研究

目的 观察脑源性神经营养因子(BDNF)及神经干细胞(NSCs)联合移植对缺氧缺血性脑损伤(HIBD)的治疗效果.方法 取新生24 h的Wistar大鼠海马NSCs进行培养、鉴定.选7日龄Wistar大鼠制备HIBD动物模型,模型制备后7 d行损伤侧侧脑室移植.将实验大鼠随机分为6组:正常组、模型组、假移植组、NSCs移植组、BDNF组、BDNF+NSCs移植组(联合移植组).移植后4周进行功能实验,取脑组织进行免疫组化免疫荧光检测.结果 从新生大鼠海马可成功培养出NSCs.Y迷宫实验结果:NSCs移植组达到学会的次数及正确记忆次数(163±11.60,5.00±1.13)n;BDNF组(150.00±8.94,5.17±1.47)n;BDNF+NSCs移植组(111.25±13.56,6.23±1.60)n.悬吊实验结果:NSCs移植组平均(30.10±11.8)s;BDNF组(36.25±10.98)s;BDNF+NSCs移植组(43.6±10.56)s.斜坡试验结果:NSCs移植组(20.3±8.25)s;BDNF组(14.33±2.88)s;BDNF+NSCs移植组(11.17±2.48)s.表明联合移植组大鼠学习记忆能力及神经功能与NSCs移植组比较明显改善(P＜0.05),而NSCs移植组大鼠学习记忆能力及神经功能与HIBD组比较,差异无统计学意义(P＞0.05).联合移植组损伤侧额叶皮层、海马存活的NSCs数量(9.31±0.71个,10.10±1.65个)与NSCs移植组(3.33±0.24个,4.22±0.33个)比较差异具有统计学意义(P＜0.01),且NSCs分化为神经元的比例明显增多,与NSCs移植组比较,差异有统计学意义(P＜0.05).结论 BDNF与NSCs联合移植较单独NSCs移植对HIBD有更好的疗效.     

《黄帝内经》医学心理学思想的理论价值

《黄帝内经》中有丰富的对人心理现象的描述,其医学心理学思想示后人以规矩,对于认识生命现象、分析病因病机、规范诊断原则、指导疾病治疗、用以养生防病等都具有重要的理论价值。     

Species differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) in several organs of mice, rats, and marmosets

To clarify species differences in the metabolism of di(2-ethylhexyl) phthalate (DEHP) we measured the activity of four DEHP-metabolizing enzymes (lipase, UDP-glucuronyltransferase (UGT), alcohol dehydrogenase (ADH), and aldehyde dehydrogenase (ALDH)) in several organs (the liver, lungs, kidneys, and small intestine) of mice (CD-1), rats (Sprague–Dawley), and marmosets (Callithrix jacchus). Lipase activity, measured by the rate of formation of mono(2-ethylhexyl) phthalate (MEHP) from DEHP, differed by 27- to 357-fold among species; the activity was highest in the small intestines of mice and lowest in the lungs of marmosets. This might be because of the significant differences between Vmax/Km values of lipase for DEHP among the species. UGT activity for MEHP in the liver microsomes was highest in mice, followed by rats and marmosets. These differences, however, were only marginal compared with those for lipase activity. ADH and ALDH activity also differed among species; the activity of the former in the livers of marmosets was 1.6–3.9 times greater than in those of rats or mice; the activity of the latter was higher in rats and marmosets (2–14 times) than in mice. These results were quite different from those for lipase or UGT activity. Because MEHP is considered to be the more potent ligand to peroxisome proliferator-activated receptor involved in different toxic processes, a possibly major difference in MEHP-formation capacity could be also considered on extrapolation from rodents to humans.     

小儿进行性脊髓性肌萎缩83例临床分析

目的总结小儿进行性脊髓性肌萎缩(SMA)各类型的临床表现、神经电生理及肌肉病理特点,提高对本病的认识水平并探讨基因诊断及产前诊断的临床意义。方法83例各型SMA患儿,男55例,女28例,年龄1d∽14岁,平均23．7个月,对本组病例进行临床特点、神经电生理、肌肉病理及基因分析。结果83例SMA临床分为3型,其中SMA-1型60例,SMA-2型19例,SMA-3型4例,3型SMA各有特点,但临床均表现为近端肌肉无力,肌张力低下。本病为单纯运动神经元受累,故患儿电生理表现均为神经源性损害而无感觉神经受累及明显的运动神经传导速度减慢;2例行肌活检显示大组萎缩肌纤维;13例行运动神经元生存基因(SMN)检测,11例外显子7和8联合缺失,1例仅第7外显子缺失,1例仅第8外显子缺失。结论根据临床特点,电生理,肌肉病理及基因诊断可与其他松软婴综合征鉴别,而能确诊SMA。基因诊断可为产前诊断提供依据,达到预防本病发生的目的。     

In vivo optical imaging of tone-evoked activity in the dorsal cochlear nucleus with a voltage sensitive dye

We investigated the use of optical imaging for observing the spatial patterns of neural activation in the dorsal cochlear nucleus (DCN) of hamsters during tonal stimulation. The patterns of activation were studied in the DCN, in vivo, following application of a voltage sensitive dye, Di-2-ANEPEQ, to the DCN surface. Beginning 60-90 min following dye application, tones were presented to the ipsilateral ear. Electrophysiological recordings after dye application revealed no significant toxicity of Di-2-ANEPEQ that affected the frequency-tuning properties of DCN neurons. We examined areas of activation in response to each of a series of test stimuli consisting of pure tones ranging in frequency from 2 to 20 kHz. For each stimulus condition, images were collected over a stimulus interval of 400 msec and averaged over 32 stimulus repetitions. These images revealed areas of activation with definable epicenters. The epicenters shifted from lateral to more medial locations on the DCN surface with increases in stimulus frequency. Comparison with electrophysiological data indicated a close parallel between the tonotopic gradient defined by optical imaging and that defined by the distribution of characteristic frequencies. The principal temporal and spatial features of these optical responses are described.     

Anesthesia management for the child with Sanjad-Sakati syndrome

Sanjad-Sakati syndrome (SSS) is a rare genetic disorder characterized by congenital hypoparathyroidism, hypocalcemia and hyperphosphatemia, seizures, severe intrauterine and postnatal growth failure, dwarfism, mental retardation, dysmorphic features including retromicrognathia and abnormal dentition and increased susceptibility to infection. It is mainly confined to children in the Middle-East countries. We report the anesthesia management of a 12-year-old boy with SSS for dental treatment, and discuss the anesthesia implications of this disorder.