Antimicrobial effect of radiation of ionized plasma

Department of Experimental Surgery, Interfaculty Laboratory Complex, and Department of Clinical Laboratory Diagnosis, Postgraduate Medical Faculty, N. I. Pirogov Second Moscow Medical Institute. (Presented by Academician of the Academy of Medical Sciences of the USSR V. V. Kupriyanov.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 110, No. 10, pp. 413–145, October, 1990.     

使用瞳孔孔径动态变化测量分析装置定量检测正常人瞳孔对光反射

目的：定量观察正常人瞳孔对光反射。方法：以瞳孔孔径动态变化测量分析装置连续摄录并且分析127名正常人瞳孔直径，分析不同年龄的瞳孔对光反射的变化；结果：不同年龄组的瞳孔对光反射的瞳孔收缩速率和收缩时间不同，年龄越大，瞳孔收缩速率越慢。年龄越小，瞳孔收缩时问越短。结论：瞳孔孔径动态变化测量分析装置能较好地定量检测人瞳孔对光反射。     

Sleep and daytime sleepiness in retinitis pigmentosa patients

OBJECTIVE: We examined sleep, daytime sleepiness and the ability to stay awake during the day in patients affected with retinitis pigmentosa (RP), to further delineate the role of photoreceptors in the circadian cycle. METHODS: Twelve individuals diagnosed with RP (40 +/- 8 years) And 12 normally sighted healthy individuals (39 +/- 7 years) matched for age, body mass index (BMI) and sex were selected for the study. Participants had their sleep recorded on two consecutive nights and were monitored on the two following days. On the first day, their ability to stay awake and on the second, their sleep propensity were assessed using the Maintenance of Wakefulness Test (MWT) and the Multiple Sleep Latency Test (MSLT), respectively. Self-report measures were obtained using the Pittsburgh Sleep Quality Index (PSQI), the Epworth Sleepiness Scale (ESS), and the Toronto Hospital Alertness Test (THAT). RESULTS: Subjective daytime sleepiness (ESS: 9 +/- 5 vs. 6 +/- 4, P=0.053) and objectively measured sleep propensity (MSLT: 10 +/- 5 vs. 17 +/- 3 min, P < 0.000) were significantly higher in RP patients than controls, whilst their alertness (THAT: 29 +/- 9 vs. 38 +/- 7, P=0.016) and ability to stay awake (MWT: 21 +/- 9 vs. 29 +/- 2 min, P=0.006) were significantly reduced. Retinitis pigmentosa participants had more disturbed nighttime sleep, with significantly more awakenings (arousal index: 14 +/- 8 vs. 8 +/- 6 h, P=0.039), and tended to have less rapid eye movement (REM) sleep (19 +/- 5 vs. 22 +/- 3%, P=0.094). CONCLUSION: Patients with RP have increased daytime sleepiness, reduced alertness and more disturbed nighttime sleep of poorer quality than their normally sighted counterparts, suggesting an influence of photoreceptor degeneration on the circadian cycle.     

He-Ne激光、微波、红外线和TDP灯照射对血粘度与红细胞影响的比较研究

目的比较研究He-Ne激光、微波、红外线和TDP灯照射对高血粘的影响,探索能否利用He-Ne激光、微波、红外线和TDP灯照射降低血液粘度,改善血循环.方法每份血样等分5份,1份作对照,对另外4份分别作He-Ne激光、微波、红外线和TDP灯照射处理.结果高血粘血样经He-Ne激光、微波、红外线和TDP灯照射作用后,全血表观粘度η降低(P＜0.01)、红细胞电泳率EPM加快(P＜0.01),血沉速度ESR提高(P＜0.01),一氧化氮、全血黏度、全血还原黏度降低(P＜0.01).红细胞变形指数降低(P＜0.01),红细胞聚集时间增加(P＜0.01),红细胞压积降低(P＜0.01),纤维蛋白降低(P＜0.01).结论He-Ne激光、微波、红外线和TDP灯照射能改善高血粘血样的流变特性.而且激光效果最好,依次是TDP灯、微波和红外线.进一步了解物理与临床治疗的密切关系.     

黑光灯对银屑病血液流变性的影响与疗效

黑光灯对银屑病血液流变性的影响与疗效刘夕茹１李海生２于霖２银屑病是一种常见、易复发的慢性炎症性皮肤病，人们曾对其发病机制以及治疗有过大量探索。本文通过使用黑光灯对其治疗前后的血液流变学指标的检测和综合分析，对该病作进一步探讨。１资料与方法１．１病例选...     

Use of monoclonal antibodies against myosin light chains 1 to detect the corresponding antigen in blood of patients with myocardial infarction

Research Institute of Human Genetics, All-Union Medical Genetics Research Center, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. D. Ado.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 112, No. 10, pp. 416–417, October, 1991.     

Changes of energy metabolism,myosin light chain composition,lactate dehydrogenase isozyme pattern and fibre type distribution of denervated fast-twitch muscle from rabbit after low frequency stimulation

The influence of low frequency (8–10 Hz) electrical stimulation on denervated fast-twitch muscle from rabbit was investigated. Prolonged direct stimulation of denervated muscle resulted in higher oxidative enzyme activities. Furthermore, single fibre analyses for succinate dehydrogenase showed a more uniform distribution of activity in stimulated-denervated muscle when compared to normal muscle. As was also the case following stimulation of innervated muscle, glycolytic enzymes were decreased in activity and the LDH-isozyme pattern also shifted towards heart type. No change of the myosin light chain pattern could be observed after 56 days of stimulation.This study was supported by the Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 138, and by grant NI 204-2-2. H. R. is grateful to Dr. Dirk Pette for his guidance and encouragement during this work, which was done in Dr. Pette's laboratory at the University of Konstanz     

Immunohistochemical localization of substance P and substance P receptor (NK1) in the olivary pretectal nucleus of the rat

The olivary pretectal nucleus (OPN) is the first central nucleus in the pupillary light reflex arc (PLR). Substance P (SP) is a neuropeptide present in the OPN. The present immunohistochemical study, performed at the ultrastructural level, aimed to determine the synaptic localization of SP and SP receptor in the OPN. Three types of SP-positive terminals were found. The most abundant type was of retinal origin, characterized by electron-lucent mitochondria and round vesicles, organized in glomerular structures, making asymmetric synaptic contacts with dendrites, and profiles containing pleomorphic vesicles, also making synaptic contacts with dendrites. The second type of SP-immunoreactive terminal contained electron-dense mitochondria and pleomorphic vesicles. This type made symmetric synaptic contacts and may originate from the ventral part of the lateral geniculate nucleus. The third type of SP-immunoreactive terminals contained electron-dense mitochondria, clear round vesicles, and made an asymmetric synaptic contact. This type originates from the contralateral OPN. SP receptors of the NK1 subtype were revealed to be on dendrites and were part of the glomerular-like arrangement. On account of the present observations, it can be concluded that retinal projections to the OPN use SP as a neuromodulator and synapse on NK1 receptor-containing dendrites of large neurons projecting to the Edinger-Westphal nucleus. Since SP also modulates the parasympathetic component of the PLR, we postulate that SP plays a modulating role in all components of the PLR.     

Clonorchis sinensis: molecular cloning and localization of myosin regulatory light chain

One cDNA clone was purified from an adult Clonorchis sinensis cDNA library, and its deduced polypeptide sequence was found to be homologous with myosin regulatory light chain (MRLC) of invertebrates and vertebrates. Two amino-acid residues, Thr and Ser, were conserved at the phosphorylation sites that regulate the function of MRLCs. Recombinant C. sinensis MRLC (rCsMRLC) protein was produced and purified from Escherichia coli, and mouse anti-CsMRLC immune sera recognized a protein of molecular weight 24 kDa from a soluble protein preparation of C. sinensis. The CsMRLC protein was immunohistochemically localized to the muscle fibers of the subtegumental muscle layer and to the muscles of oral and ventral suckers. However, the rCsMRLC protein proved to be less useful antigen for the serodiagnosis of human clonorchiasis.The nucleotide sequence reported herein was submitted to GenBank and assigned accession number AY519356.     

Favourable associations between the myosin heavy-chain and light-chain isoforms in human skeletal muscle

Histochemical and biochemical analyses were performed in order to examine the relationship between myosin light-chain (LC) isoforms and fibre-type distributions in whole human skeletal muscle. Muscle biopsies were obtained from the vastus lateralis muscle in six healthy men, and analysed for the relative area occupied by each fibre type (percentage of fibre type area) and the molar ratio of each LC isoform. The percentage of type I fibre area was positively correlated with the molar ratio of slow LC (LC1s and LC2s) to total LC. The regression line was located below the line of unity. Also, the ratio of percentage of type II fibre area to that of type II fibre area was positively correlated with the molar ratio of the fast alkali LC LC1f to fast alkali LCs LC1f and LC3f. These results support previous study, having shown that in human skeletal muscle some type I fibres express various amounts of fast LC in addition to slow LC and suggest that fast myosin heavy-chain HCII a is favourably associated with LC1f, whereas HCIIb is favourably associated with LC3f.