利用多种指标对哈尔滨市人群生命质量的评价

采用多种指标综合分析 ,对哈尔滨市区的人群生命质量进行了评价     

磁极化生物平衡离子吸附疗法治疗老年人心脑血管病危险因素的临床研究

目的 探讨磁极化生物平衡离子吸附降脂降粘疗法(也称之为洗血疗法)对老年心脑血管病危险因素的临床疗效。方法 应用磁极化生物平衡离子吸附疗法对22例老年患有心脑血管病危险因素的患者进行治疗,并对临床各项指标进行对照比较。结果 22例患者治疗前后的血流变学指标、血脂等均有明显改善。治疗前患者的TCHO(胆固醇)、TG(甘油三酯)分别为(6.21±1.14)mmol/L和(6.78±5.13)mmol/L;治疗后分别为(4.36±0.99)mmol/L和(2.41±2.10)mmol/L,P<0.05,均显著降低。治疗前患者的HCT(红细胞压积)、PFC(纤维蛋白)、NP(血浆粘度)、NR(全血粘度)、VAI(细胞聚集指数)分别为(0.441±0.042)V、(4.11±1.78)g/L、(3.86±1.98)mpa.s、(5.68±0.79)mpa.s和0.89土0.23;治疗后分别为(0.344±0,061)V、(2.21±1.12)g/L、(1.49±0.40)mpa.s、(3.21±0.56)mpa.s和0.40±0.15,治疗前后对照比较,P<0.02。结论本方法对老年人心脑血管病危险因素的治疗是有效的。     

鼠和兔中流行性感冒病毒抗体的制备及测定

报道了流行性感冒病毒抗体的制备及其抗体价的检测方法。利用鼠、兔对流行性感冒(IFV)抗原的免疫反应制备抗体；酶联免疫法(ELISA)测定抗体价。结果显示：Freund佐剂与抗原的混合乳液，具有流行性感冒病毒的抗原特异性，且能够增强抗原的免疫原性，并改变宿主的免疫反应原性。     

10Kg以下法乐氏四联症的急诊手术治疗

目的 总结低体重法乐氏四联症急诊或亚急诊手术的治疗经验。方法 15例体重小于10kg的法乐四联症患儿因合并有严重缺氧发作、感染性心内膜炎、心衰等，经积极的心内科治疗后转行急诊或亚急诊根治手术。结果 死亡1例，病死率6．7％；存活14例，但术后有较多、较重的并发症，其中1例的术前脑缺氧损害后遗表现仍然明显。结论 急诊或亚急诊根治术是救治合并有危急并发症低体重法乐氏四联症患儿的有效方法。     

颞骨岩部胆脂瘤

目的：探讨岩部胆脂瘤的临床特征及手术方法。方法：经迷路前上径路手术治疗1例，经迷路-耳蜗径路手术治疗8例。结果：术后8例随访2-9年，局部无胆脂瘤复发，其中2例面神经全程减压，术后未能恢复；1例术后2个月出现面肌运动，7个月后恢复正常，1例术后仅2个月，有待随访。结论：根据病变部位及侵犯范围选择适当的手术途径；术腔内填塞脂肪，封闭外耳道可防止脑脊液外漏。     

内耳畸形患儿的人工耳蜗植入

目的：探讨几种内耳畸形患儿的人工耳蜗植入效果。方法：对10例内耳结构异常的感音性耳聋患儿进行了人工耳蜗植入。结果：术后随访0．5～4．0年，10例基本达到了较满意的听觉言语恢复效果。结论：内耳畸形息儿行人工耳蜗植入，应严格进行术前听力学与影像学的评估，严格掌握手术的适应证，可以达到较满意效果。     

原发性高血压肝阳上亢证证治规律探讨

肝阳上亢证是原发性高血压的常见证型 ,与胰岛素抵抗增强、酪氨酸羟化酶基因过度表达、血小板α-颗粒膜蛋白分泌异常、血管内皮细胞损伤、红细胞变形能力降低、微循环障碍和垂体 -性腺激素分泌异常密切相关 ,其病机演变有内在规律 ,证候表现有自身特点 ,治疗以平肝潜阳为法。从而为平肝潜阳法治疗原发性高血压提供了理论依据     

Loss of regulation of circulating 1,25-dihydroxyvitamin D with paradoxically decreased serum phosphate levels in individuals with recurrent kidney stones

A low serum phosphate concentration is characteristic in individuals in whom kidney stones form, this being related to serum 1,25-dihydroxyvitamin D, parathyroid hormone and urinary phosphate excretion. In order to determine whether these parameters are related to recurrence of stone formation, they were analyzed in single and recurrent stone formers as well as controls. An inverse correlation between serum levels of phosphate and 1,25-dihydroxyvitamin D was observed in control subjects, indicating that a drop in serum phosphate results in upregulated circulating 1,25-dihydroxyvitamin D level in controls. While the circulating low phosphate level upregulated the 1,25-dihydroxyvitamin D level in single stone formers, the elevation was less than expected from the drop in serum phosphate in recurrent stone formers. The results thus suggest that loss of upregulation of 1,25-dihydroxyvitamin D by serum levels of phosphate might be important for stone formation. The possibility of deregulation of 1,25-dihydroxyvitamin D to maintain physiological requirements in stone formers and prevent further nephrolithiasis therefore warrants attention. Received: 27 January 1999 / Accepted: 25 June 1999     

Assessing equine sperm-membrane integrity

The swelling of cells in a hypo-osmotic medium has been described as an important criterion for assessing the functional integrity of the sperm plasma membrane. The resistance of equine spermatozoa to osmolarity changes was studied by extending 98 semen samples collected from nine stallions in media at five osmolarities (300, 200, 150, 100, and 50 mOsmol l(-1)). The response of the cells was measured by the spermatocrit technique and eosin staining. Spermatocrit determines the increase on spermatozoal volume under hypo-osmotic conditions, a sign of functional integrity of sperm plasma membrane, whereas the eosin staining evaluates the viability of spermatozoa. A significant positive correlation (P<0.01) was observed between spermatocrit values and percentage of eosin-unstained cells. Spermatocrit measurements and eosin staining proved to be useful methods to evaluate the integrity of sperm plasma membrane under hypo-osmotic conditions and could be used as an additional criterion to predict semen preservation ability.     

豚鼠柯替氏器支持细胞的分离和鉴定

目的：建立豚鼠柯替氏器支持细胞的分离方法，并观察活性支持细胞的形态特征。方法：对豚鼠基底膜用Ⅳ型胶原酶(1mg/ml)消化和微机械分离法获得柯替氏器单离支持细胞，在倒置显微镜下观察细胞的活性和形态特征并记数。结果：不同支持细胞在倒置显微镜下各有其形态特征，第一、二排Deiters细胞为逗点状，第三排为弓状：Hensen细胞为长圆形，半透明的胞质内含有数个脂质样颗粒；外柱细胞呈哑铃状，两端对称性圆球形，中间可见清晰的微管；内柱细胞呈“长勺形”，底部为圆球状，顶部逐渐向外侧弯曲成杯口样结构。每个豚鼠耳蜗可分离出单离Dieters细胞1～4个，Hensen细胞10～20个，内柱细胞和外柱细胞分别约80～100个。结论：Ⅳ型胶原酶消化和微机械分离法可分离出豚鼠柯替氏器的Deiters细胞、Hensen细胞和内、外柱细胞，可为在细胞和分子水平对单离活性支持细胞进行形态和功能变化的研究提供一种简便实用的方法。