中药内外结合穴位埋线治疗黄褐斑67例

目的:观察中药内外结合穴位埋线治疗黄褐斑的临床疗效。方法:将67例门诊病人采用中药辨证口服结合中药玉容散加减外敷及穴位埋线治疗。结果:治疗3个疗程后,基本治愈45例,占67.2%,总有效率97.0%,各证型间疗效比较,差异无显著性意义(P>0.05)。结论:中药内外用结合穴位埋线治疗黄褐斑疗效是满意的,临床值得推广应用。     

DNA and RNA Isolated from tissues processed by microwave-accelerated apparatus MFX-800-3 are suitable for subsequent PCR and Q-RT-PCR amplification

Over the past decade, methods of molecular biology have appeared in diagnostic pathology and are routinely applied on formalin-fixed, paraffin-embedded histological samples, processed via conventional embedding methods. Due to its reagent- and cost-effectiveness, embedding techniques that utilize microwave acceleration in one or more steps of histoprocessing are increasingly used by numerous laboratories. The demand arises that tissues processed this way should also be suitable for the requirements of molecular pathology. In this study, both conventionally embedded and MFX-800-3 machine-processed tissue samples from the same source were used for isolation of DNA and RNA and for performing PCR and real-time PCR. PCR amplification of the β-globin gene, as well as the real-time PCR amplification of the ABL mRNA was successful in all cases. Our conclusion is that samples processed by the vacuum assisted automatic microwave histoprocessor MFX-800-3 are perfectly applicable for DNA and RNA isolation and provide appropriate templates for further PCR and realtime PCR studies.     

河南省三甲医院硕士学位护士科研能力对职业嵌入的影响:自我效能的中介作用

目的 调查硕士学位护士自我效能在科研能力与职业嵌入中的中介作用。方法 选择河南省8所三级甲等医院的397名硕士学位护士为研究对象,采用一般资料调查表、护理人员科研能力自评量表、一般自我效能量表以及职业嵌入量表对其进行调查。结果 硕士学位护士科研能力得分为(82.14±19.68)分,处于高水平。科研能力、自我效能与职业嵌入均呈正相关(r=0.329,P<0.001;r=0.448,P＜0.001),科研能力总分与自我效能总分呈正相关(r=0.472,P<0.001)。自我效能在科研能力与职业嵌入之间起部分中介效应,中介效应值为0.111,占总效应的52.11%。结论 自我效能在科研能力和职业嵌入之间具有部分中介作用,医院管理者在制定硕士学位护士职业嵌入干预措施时,可以通过提升科研能力和自我效能的方式来提高其职业嵌入水平,稳定高层次护理人才队伍。     

穿刺小标本改良石蜡块制作方法及评价

目的: 改良现有琼脂预包埋石蜡包埋法,评价该包埋法对空芯针穿刺活检小标本组织完整性、组织学形态、蛋白质和DNA检测的影响。方法: 取10例口腔黏膜鳞癌空芯针穿刺活检标本,分别进行成型包埋模具的改良琼脂预包埋石蜡包埋法及常规石蜡包埋法,前者缩短脱水时间至3.5 h,后者为12 h。样本处理后,分别行H-E染色、组织学形态、免疫组织化学（IHC）和DNA荧光原位杂交（FISH）等检测,并对检测结果进行对比分析。采用GraphPad Prism 9软件包对数据进行组间t检验。结果: 改良琼脂预包埋法较琼脂预包埋法降低了操作难度,易于推广。与常规石蜡包埋法相比,组织脱水时间显著缩短（P<0.001）,对镜下组织学形态及后续IHC和FISH检测无影响。结论: 改良琼脂预包埋石蜡包埋法满足临床病理诊断对组织处理的要求,是值得推广的空芯针穿刺活检标本组织包埋法。     

PPDO可吸收线双平面皮下埋置在面部年轻化治疗中的应用效果

探究PPDO可吸收线双平面皮下埋置应用于面部年轻化治疗中的效果。方法 选取2022年1月-12月我院收治的60例面部皮肤松弛患者为研究对象,随机分为对照组和观察组,每组30例。对照组采用PPDO锯齿线单平面皮下埋置,观察组采用PPDO锯齿线与PPDO螺旋线相结合双平面皮下埋置,比较两组面部松弛改善效果及不良反应发生情况。结果 观察组面部松弛改善优良率为96.67%,高于对照组的80.00%（P ＜0.05）;观察组不良反应发生率为3.33%,低于对照组的6.67%,但差异无统计学意义（P ＞0.05）。结论 PPDO可吸收线双平面皮下埋置在面部年轻化治疗中的应用效果确切,可有效改善面部松弛情况,提升面部肌肤紧致度,进而有利于提高面部美观度,实现面部年轻化。     

推拿按揉法加背俞穴埋线治疗变应性鼻炎临床研究

目的:探讨推拿按揉法加背俞穴埋线治疗变应性鼻炎的临床效果。方法:选取2017年6月~2019年5月收治的变应性鼻炎患者88例,随机分为对照组和观察组各44例,均给予服用枸地氯雷他定,观察组在此基础上给予推拿按揉法加双侧背俞穴埋线治疗,比较两组临床疗效、治疗前后鼻部症状和体征积分,IgE、TNF-α、IL-4、IL-12水平及临床复发率。结果:观察组总有效率为93.18%,高于对照组的79.55%(P<0.05);观察组鼻部症状体征积分改善程度优于对照组(P<0.05);观察组IgE、TNF-α、IL-4、IL-12水平改善程度优于对照组(P<0.05);观察组复发率为4.70%,低于对照组的41.19%(P<0.05)。结论:推拿按揉法加背俞穴埋线治疗变应性鼻炎可显著减轻临床症状和体征,改善患者炎性因子水平,并有助于降低复发率。     

荧光标记法比较4种表面处理方式种植体骨矿化沉积率的研究

目的荧光标记法观察比较4种表面处理方式种植体周围骨矿化沉积率的差异。方法微创拔除4只Beagle犬双侧下颌4颗前磨牙,待拔牙创愈合3个月后,植入奥齿泰种植体40颗,其中机械形态表面(ma-chined morphology,MM)组4颗,喷砂加酸蚀(sand blasted with alumina and acid etched,SA)组、可吸收性研磨介质(resorbable blasting media,RBM)组和生物化学喷砂酸蚀组(biochemistry sand blasted with alumina and acid etched,Bio-SA)组各12颗,愈合3个月,处死前13、14 d和3、4 d分别注射盐酸四环素和钙黄绿素,制取标本并置于70%酒精固定,采用塑料包埋技术制作骨磨片,荧光显微镜下观察,测量骨矿化沉积率。结果螺纹间及远离螺纹区域新骨矿化沉积率在4组间的差异均没有统计学意义(P>0.05);单独比较每组种植体螺纹间及远离区域骨矿化沉积率的差异性,RBM组及SA组组内差异没有统计学意义(P>0.05),而MM组和Bio-SA组组内差异具有统计学意义(P<0.05)。结论采用塑料包埋技术,通过荧光标记法能成功观察到新骨矿化沉积的情况;种植体植入3个月后,4种不同粗糙程度的种植体螺纹间及远离螺纹区域骨矿化沉积率没有差异性,而经机械形态表面处理的种植体和生物化学喷砂酸蚀处理的种植体,螺纹间骨矿化沉积比远离螺纹区域快。     

Book Reviews

Abstract

In order to simultaneously and specifically stain mast cells and eosinophils in a study involving large numbers of paraffin-embedded intestinal samples from horses, a modified Congo red-acidified toluidine blue (CTB) technique was devised. This stain combined convenience of preparation with excellent staining of the two cell types. Mast cells appeared a dark bluish-purple, while eosinophils appeared a bright yellow-orange. Individual granules in both cell types were easily appreciated.     

Histologists,Microtomy, Chronic Repetitive Trauma,and Techniques to Avoid Injury: II. A Physical and Rehabilitation Medicine Physician's Perspective

Abstract

In the first of this series of articles, a national survey of histotechnologists revealed an unexpectedly high incidence of cumulative trauma disorders (CTD) or carpal tunnel syndrome (CTS). Previous reports for CTS have shown incidences ranging from 125 to 515 per 100,000 population in random surveys. Using a focused survey of only American Society of Clinical Pathologist registered technologists, an estimated population incidence of 2,200 per 100,000 had medically treated CTS, 6,300 per 100,000 had medically treated CTD and 14,800 per 100,000 had chronic pain symptomatology.

In this article, members of the Department of Physical and Rehabilitation Medicine discuss the etiology and potential remedies for reducing the incidence of CTD or CTS. ( The J Histotechnol 18:327, 1995).