Natural rubber latex and chestnut allergy: cross‐reactivity or co‐sensitization?

BACKGROUND: Chestnut and natural rubber latex (NRL) allergy are often associated in the latex-fruit syndrome. AIM OF THE STUDY: To establish whether the concurrent NRL and chestnut IgE antibody reactivity are the results of co-sensitization or cross-reactivity. METHODS: Sera from 19 patients with chestnut- and NRL-specific IgE were selected and tested for reactivity with recombinant (r) latex allergens. Cross-reactivity was explored by IgE-inhibition experiments using chestnut or NRL allergens as solid phase on ImmunoCAP. RESULTS: IgE-antibodies were detected to rHev b 6.01 (prohevein) in 58% of the sera, to rHev b 5 in 32%, to rHev b 12 in four of 13 sera, to rHev b 7.02 and rHev b 11 in four, and to rHev b 1 in two of 19 sera. rHev b 8-IgE antibodies were found in nine sera (47%), whereas six displayed mono-sensitization to rHev b 8 with regard to our test panel. Three of 16 sera showed IgE to cross-reactive carbohydrate determinants. In most sera recognizing rHev b 5 and/or rHev b 6.01 as major allergens the IgE-reactivity to NRL remained unaffected by chestnut extract and chestnut-IgE remained unaffected by NRL extract. Conversely, in sera with rHev b 8 as dominant allergen IgE-binding to NRL was nearly completely inhibited by chestnut and vice versa. IgE-binding to rHev b 8 was abolished by chestnut extract. CONCLUSIONS: Although patients have concomitant IgE antibody reactivity to chestnut and NRL, cross-reactivity could be demonstrated mainly in those patients with IgE to Hev b 8 (profilin) from NRL.     

针刺加叩刺治疗女性2型糖尿病阴虚夹瘀型患者临床观察

目的:观察针刺加扣刺治疗女性2型糖尿病阴虚夹瘀型患者的临床疗效。方法:92例女性2型糖尿病阴虚夹瘀型患者依据证候轻重特征将阴虚夹瘀患者分为瘀证为主的阴虚夹瘀型(简称组1)和阴虚为主的阴虚夹瘀型(简称组2)两组各46例。采用针刺加叩刺治疗,隔日治疗1次,持续治疗3个月。观察两组患者治疗前后空腹血糖(FPG)、空腹瘦素(FLP)、空腹胰岛素(FINS)、胰岛素敏感性指数(ISI)、胰岛素抵抗指数(Homa-IR)及总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-c)、高密度脂蛋白胆固醇(HDL-c)水平。取41例正常女性作为对照组,与两组患者治疗前后各项指标项比较。结果:治疗前两组FPG、FLP、FINS、Homa-IR、TC、TG、LDL均明显高于正常组水平;HDLC,ISI低于正常组水平。两组患者治疗后FPG、FLP、FINS、Homa-IR、TC、TG、LDL均较治疗前明显下降(P<0.01),ISI、HDL明显升高(P<0.01)。治疗后组1 TG、Homa-IR的改善优于组2(P<0.05)。组1总有效率为91.3%,组2总有效率为76.09%,两组比较差异无统计学意义(P>0.05)。结论:针刺加扣刺治疗女性2型糖尿病阴虚夹瘀型患者糖、脂质代谢及胰岛素抵抗具有良性调整作用,且以瘀证为主的阴虚夹瘀型在降低胰岛素、改善胰岛抵抗指数相关参数方面的作用优于以阴虚为主的阴虚夹瘀型患者。     

Inner Shell of the Chestnut (Castanea crenatta) Suppresses Inflammatory Responses in Ovalbumin-Induced Allergic Asthma Mouse Model

The inner shell of the chestnut (Castanea crenata) contains various polyphenols, which exert beneficial biological effects. Hence, we assessed the anti-inflammatory efficacy of a chestnut inner shell extract (CIE) in ovalbumin (OVA)-induced allergic asthma. We intraperitoneally injected 20 μg of OVA with 2 mg of aluminum hydroxide on days 0 and 14. On test days 21, 22, and 23, the mice were treated with aerosolized 1% (w/v) OVA in saline. CIE was administered orally at 100 and 300 mg/kg on days 18–23. CIE significantly reduced inflammatory cytokines and cells and immunoglobulin-E increased by OVA. Anti-inflammatory efficacy was revealed by reduction of inflammatory cell migration and mucus secretion in lung tissue. Further, CIE suppressed the OVA-induced nuclear factor kappa B (NF-κB) phosphorylation. Accordingly, the expression of cyclooxygenase (COX-2), inducible nitric oxide synthase (iNOS), and matrix metalloproteinase-9 (MMP-9) were decreased sequentially in lung tissues. CIE alleviated OVA-induced airway inflammation by restraining phosphorylation of NF-κB and the sequentially reduced expression of iNOS, COX-2, leading to reduced MMP-9 expression. These results indicate that CIE has potential as a candidate for alleviating asthma.     

Identification of novel saponins from edible seeds of Japanese horse chestnut (Aesculus turbinata Blume) after treatment with wooden ashes and their nutraceutical activity

Natural seeds of Japanese horse chestnut (Aesculus turbinata Blume) contain large amounts of mixed triterpenoidal saponins called escins. Recent studies have shown that escins have several biological activities including anti-inflammatory action and inhibitory effects on the absorption of ethanol and glucose. For the edible utilization of the seeds, natural seeds are usually treated with wooden ashes to remove harshness. Here, we found the novel compounds derived from escins in the edible seeds after the food processing with wooden ashes. The instrumental analyses revealed the chemical structures of escins and the derivatives. These compounds are identified as four types of deacetylescins Ia, IIa, Ib, and IIb as well as two types of desacylescins I and II. To determine their biological activity, the purified compounds were tested for their potential nutraceutical activity. The oral glucose tolerance test in mice revealed that a single oral administration of the isolated components of deacetylescins at a dose of 100 mg/kg was clearly effective in attenuating the elevation of blood glucose levels. The inhibitory effects of escins and their derivatives were in the order of escins > deacetylescins > desacylescins. Moreover, we found the inhibitory activity of those compounds on pancreatic lipase. Escins were the most potent in inhibiting the enzyme activity, and followed by desacylescins and then deacetylescins. Taken together, our results suggest the potential usefulness of novel saponins including deacetylescins and desacylescins from edible seeds as novel sources for nutraceutical foods with anti-obese effects.     

角膜栗刺异物56例临床分析

目的:探讨角膜栗刺异物的发生原因、分类、及不同治疗方法,为栗刺的诊治工作提供依据。方法:对收治的56例角膜栗刺异物患者的发病、诊断及治疗情况进行回顾性总结,分析发病时间、视力、手术方法及手术疗效。结果:男性患者多于女性,多数患者年龄<50岁。未发现复合外伤性白内障患者。82%患者可直接在门诊裂隙灯下取出栗刺。18%患者需要住院在显微镜下取出栗刺。治疗后视力好于治疗前,差异有显著意义(P<0.05)。结论:角膜栗刺外伤表现多样,不同类型的栗刺采用不同的方法可提高疗效,大大降低患者住院率和治疗费用。     

神经电生理对马栗种子提取物治疗特发性面神经麻痹的疗效分析

目的 通过神经电生理检查来观察、评定马栗种子提取物治疗特发性面神经麻痹的疗效.方法 将66例患者分成对照组和治疗组,分别给予常规治疗和常规加马栗种子提取物治疗,通过神经电生理检查神经传导速度测定评定两组疗效.结果 马栗种子提取物组分别于治疗0.5个月和1.0个月时神经传导速度与对照组比较(P＜0.05)有统计学意义,马栗种子提取物组疗效好于对照组.结论 马栗种子提取物结合常规方法治疗特发性面神经麻痹疗效显著.     

射频导入参棘软膏联合口服氨甲环酸、谷胱甘肽治疗黄褐斑疗效观察

目的: 观察射频导入参棘软膏联合口服氨甲环酸、谷胱甘肽治疗黄褐斑的疗效。方法: 门诊女性黄
褐斑64 例,根据随机原则,分为治疗组和对照组,均为32 例。对照组病人口服氨甲环酸250 mg /次,2 次/d; 谷胱
甘肽400 mg /次,3 次/d,服用16wk。治疗组在对照组基础上采用舒敏专家射频导入参棘软膏,1 次/wk,共治疗
16 次。取两组病人治疗时间点第4、8、12、16wk 进行疗效标准判定及治疗前、后黄褐斑皮损面积和严重度指数
( MASI) 评分。同时记录起效时间、复发情况及不良反应,治疗疗程结束后以问卷方式调查病人满意度。结果:
治疗组和对照组治疗各观察时间点第4、8、12、16wk 总有效率分别为15．63%、46．88%、59．38%、84．38%和12．50%、
21．88%、44．00%、62．50%。治疗组治疗疗效与对照组比较差异有显著性( P＜ 0．05) ; 治疗组治疗16wk 后平均
MASI 评分明显下降至6．15±3．25分,与对照组比较,其差异具有显著性( P＜0．05) 。结论: 射频导入参棘软膏联合
口服氨甲环酸、谷胱甘肽治疗黄褐斑的临床疗效良好,方法简单,病人耐受性好,适合临床应用。     

Effects of butanol fraction of Ziziphus mucronata root ethanol extract on glucose homeostasis,serum insulin and other diabetes-related parameters in a murine model for type 2 diabetes

Context: Ziziphus mucronata Willd (Rhamnaceae) is currently used in Nigerian traditional treatment of diabetes mellitus. However, detailed information on the antidiabetic potential of the plant parts is presently unknown.

Objectives: The present study investigated the antidiabetic effects of the butanol fraction of Z. mucronata root (ZMBF) in a type 2 diabetes (T2D) model of rats.

Materials and methods: T2D was induced in rats by feeding a 10% fructose solution ad libitum for two weeks followed by an intraperitoneal injection of streptozotocin (40?mg/kg bw) and the animals were orally treated with ZMBF 150 or 300?mg/kg bw for five days a week for four weeks. Food and fluid intake, body weight changes and blood glucose levels were monitored during the experiment while other blood and organ specific diabetes-associated parameters were measured at the end of the experiment.

Results: After four-week treatment, significantly (p?p?>?0.05) affected by the treatment of ZMBF.

Conclusion: Results of this study suggest that ZMBF treatment, at 300?mg/kg bw, possess antidiabetic activity, but could not ameliorate some diabetes-related parameters in type 2 diabetic rats.     

大椎穴合谷刺为主治疗颈源性头痛160例临床观察

目的：观察大椎穴合谷刺为主对颈源性头痛的治疗效果。方法：160例病人随机进行针刺治疗,穴位取大椎,凤池等穴。结果：针刺能够很好的缓解颈源性头痛,该方法治疗简单,方便。     

刺络拔罐为主治疗周围性面神经麻痹(肝胆湿热型)急性期60例临床观察

目的:观察刺络拔罐加针刺治疗肝胆湿热型周围性面神经麻痹急性期的临床疗效。方法:将患者随机分为观察组、对照组各60例,分别采用刺络拔罐加针刺治疗(观察组)及单纯针刺治疗(对照组)的方法,共治疗3个疗程。结果:治疗1个月后观察组症状、体征评分及疗效均优于对照组(P0.05)。结论:刺络拔罐加针刺治疗优于针刺治疗,对肝胆湿热型面神经麻痹急性期有较好的临床疗效。