高密度多孔聚乙烯人工泪管植入治疗外伤性泪道阻塞的疗效分析

目的观察内窥镜指导下高密度多孔聚乙烯（high-density Porous polyethylene,HDPP）人工泪管植入治疗外伤性泪道阻塞的疗效。方法外伤性泪小管离断患者12例（12只眼）,行高密度多孔聚乙烯人工泪管植入术,术后随访6-24个月,平均随访15个月。结果术后患者溢泪症状消失或明显改善,所有人工泪管均冲洗通畅,术后并发症包括人工泪管结膜侧管口中度肉芽增殖1例（8.3%）,管口周围结膜组织肥厚1例（8.3%）,局部不适感2例（16.6%）。结论 HDPP人工泪管植入手术能明显改善外伤性泪道阻塞患者的溢泪症状,术后无严重并发症,该手术安全有效。     

何首乌水提物对大鼠肝脏CYP1A2,CYP2E1酶活性及mRNA表达抑制作用研究

大鼠连续灌胃不同剂量的何首乌水提物(1,10g·kg-1)7 d,取肝脏制备肝微粒体,分别用Cocktail体外孵育法和实时荧光定量PCR技术观察何首乌水提物对大鼠肝脏主要CYP450酶活性及mRNA表达的影响.与空白对照组相比,1,10g·kg-1何首乌水提物组大鼠肝脏CYP2E1酶活性和mRNA的表达均受到明显抑制(CYP2E1酶活性,P＜0.01;CYP2E1的mRNA表达1 g·kg-1组P＜0.05,10 g·kg-1组P＜0.01),CYP3A1酶活性虽显示明显升高(P＜0.01),但mRNA的表达没有显著变化;10 g·kg-1何首乌水提物组大鼠肝脏CYP1A2酶活性和mRNA的表达受到明显抑制(P＜0.01).     

Evaluation of protective effects of Chi-Zhi-Huang decoction on Phase I drug metabolism of liver injured rats by cocktail probe drugs

AIM OF THE STUDY: Chi-Zhi-Huang decoction (PGR) is one of the traditional Chinese medicine (TCM) preparations with unique effect on withdrawing jaundice and has been used to treat icteric patients in China for many years. In this research, we aim at to evaluate the potential activity of PGR in restoring hepatic drug metabolism in a damaged liver. MATERIALS AND METHODS: A cocktail approach with caffeine (10mg/kg), dapsone (10mg/kg) and chlorzoxazone (20mg/kg) respectively as probe drug of cytochrome P450 (CYP) isoform of CYP 1A2, 3A4 and 2E1 was used to evaluate its possible effects on Phase I oxidative metabolism. Pretreated with three dosages of PGR water extract (0.75, 1.5 and 3g/kg, po) for 5 days, male Wistar rats (220-240 g) were intoxicated by phenylisothiocyanate (PITC, 100mg/kg, po) 24h before probes intravenous injection. The pharmacokinetics of the probes in the blood was determined simultaneously by HPLC, and their non-compartmental parameters were used to evaluate the metabolic difference among the groups. Moreover, the levels of liver enzymes (ALT, AST, ALP) and bilirubins were also measured for insight of liver function. RESULTS: The findings in this study suggest that PGR induces CYP 3A4, does not have much effect on CYP 2E1, and inhibits CYP 1A2 at high dosage. CONCLUSION: The current pharmacokinetic approach allowed the protective effects of PGR on oxidative drug metabolism in damaged liver to be systemically examined and will certainly help in the explanation of synergistic effect of the composites formula.     

泪道激光探通联合泪道植管术治疗泪道阻塞的疗效观察

目的：探讨泪道激光探通联合泪道植管术治疗泪道阻塞的治疗效果。方法选择2012年3月至2014年3月期间我院眼科收治的泪道阻塞患者80例(140眼)作为研究对象,根据手术方法将其分为研究组(行泪道激光探通联合泪道植管术治疗)40例(70眼)与对照组(行单纯行泪道激光探通术治疗)40例(70眼)。随访6个月,观察两组患者的临床治疗效果,以及术后并发症情况。结果研究组治疗的总有效率为95.71%,显著高于对照组的82.86%, P<0.05。两组并发症发生率对比差异无统计学意义(P>0.05)。结论泪道激光探通联合泪道植管术治疗泪道阻塞疗效确切,操作简单,并发症少,适于临床推广与应用。     

Applicability Assessment of Different Materials for Standards Ensuring Comparability of Optical and Tactile Coordinate Measurements

Multisensor CMMs are systems with an established position on the market, but their popularity still grows, as they provide access to the advantages offered by tactile and contactless measurement methods. Yet there are still questions of the comparability of results obtained using the optical and tactile operation modes of multisensor system. This phenomenon can be assessed by measuring appropriate gauges, most often reference rings or spheres. Due to the completely different nature of probing processes for tactile and contactless measurements, the material from which reference object is made may significantly affect measurement results. In order to assess the influence of this factor on measurement accuracy, three reference spheres made from different materials were measured on optical multisensor CMMs. Measurements involved tactile measurements as well as optical measurements made using different probing systems: a video probe and white light sensor. Results obtained from performed experiments show large differences depending on the material used for spherical standard production. On the basis of obtained results, it can be stated that the best material for a reference object that can be used for comparability tests of tactile and optical measurements is a composite of alumina with at least one oxidic additive.     

Fabrication of Large-Area Mullite–Cordierite Composite Substrates for Semiconductor Probe Cards and Enhancement of Their Reliability

This work aims to fabricate a large-area ceramic substrate for the application of probe cards. Mullite (M) and cordierite (C), which both have a low thermal expansion coefficient, excellent resistance to thermal shock, and high durability, were selected as starting powders. The mullite–cordierite composites were produced through different composition ratios of starting powders (M:C = 100:0, M:C = 90:10, M:C = 70:30, M:C = 50:50, M:C = 30:70, and M:C = 0:100). The effects of composition ratio and sintering temperature on the density, porosity, thermal expansion coefficient, and flexural strength of the mullite–cordierite composite pellets were investigated. The results showed that the mullite–cordierite composite pellet containing 70 wt% mullite and 30 wt% cordierite sintered at 1350 °C performed exceptionally well. Based on these findings, a large-area mullite–cordierite composite substrate with a diameter of 320 mm for use in semiconductor probe cards was successfully fabricated. Additionally, the changes in sheet resistance and flexural strength were measured to determine the effect of the environmental tests on the large-area substrate such as damp heat and thermal shock. The results indicated that the mullite–cordierite composite substrate was extremely reliable and durable.     

纳米金标记DNA的生物传感器

目的利用纳米金标记DNA以提高传感器的检测灵敏度,为建立方便、快速、准确地检测食品中李斯特菌的方法提供依据。方法利用自组装法,将5′端巯基修饰的单链DNA固定在金电极上,然后与纳米金标记的探针进行杂交。结果传感器与标记有胶体金的探针杂交频率减少了207 Hz,与未标记有胶体金探针杂交频率(减少了52 Hz)相比提高了3倍。结论采用纳米金标记的探针可提高传感器的检测灵敏度,在食品安全检测中具有重要意义。     

应用多重连接探针扩增技术行脊髓性肌萎缩症产前诊断的结果分析与遗传咨询指导

目的:对脊髓性肌萎缩症(spinal muscular atrophy, SMA)家系进行产前诊断,为SMA产前分子诊断提供遗传咨询指导意见。方法:纳入2016年至2019年在本院产前诊断中心就诊的21个家系开展研究,应用多重连接探针扩增(multiplex ligation-dependent probe ampli...     

一种无RNA纯化步骤TaqMan RT-PCR方法用于快速检测登革病毒

目的 建立一种简便、快速的登革病毒核酸检测方法.方法 设计合成LNA探针,替代MGB探针,建立一步法TaqMan RT-PCR方法,并用已知的登革病毒标准毒株进行方法的优化;用商品化的纯化试剂盒和简单的热释放法获取登革病毒细胞培养液和模拟含登革病毒血清中RNA,以优化的TaqMan RT-PCR方法进行检测,比较有或无RNA纯化步骤检测的敏感性和重复性.结果 LNA探针TaqMan RT-PCR有较好的PCR扩增效率(104%)、较广的线性范围(10 0～10-7样品稀释度)、较高的敏感性(0.003TCID50/反应)和较好的重复性(CV值小于4.53%),新方法检测经简单热释放RNA的细胞培养液和模拟含登革病毒血清中登革病毒也有较高的敏感性(0.03TCID50/反应)和较好的重复性(CV值小于6.36%).结论 新型LNA探针可替代MGB探针用于TaqMan RT-PCR反应,简便的热释放RNA方法结合高敏感性的TaqMan RT-PCR可满足登革病毒快速检测的需要.     

经皮肾镜钬激光碎石与双导管超声碎石的比较研究

目的：比较经皮肾镜钬激光碎石与双导管超声碎石的疗效及安全性。方法回顾性分析2012年10月~2014年12月我院泌尿外科采用经皮肾镜碎石术治疗136例肾结石患者的临床资料,按碎石取石方式不同分为钬激光碎石组(A组66例)和双导管超声碎石组(B组70例),比较两组患者手术时间、失血量、术后感染发生率、Ⅰ期清石率的差异。结果A组手术时间为(85.36±21.52)皂蚤灶,术中出血量为(154.18±33.25)皂蕴,术后感染发生率为13.64豫,Ⅰ期清石率为90.91豫;月组手术时间为(76.15±20.36)皂蚤灶,术中出血量为(85.66±27.68)皂蕴,术后感染发生率为2.86%,Ⅰ期清石率为92.86%。两组手术时间、失血量、术后感染发生率比较差异有统计学意义(孕<0.05),Ⅰ期清石率比较差异无统计学意义(孕>0.05)。结论经皮肾镜双导管超声碎石术治疗肾结石手术时间短,术后感染、出血并发症少,疗效及安全性优于经皮肾镜钬激光碎石术。