痢疾杆菌耐药情况与质粒谱分析研究

目的 了解红河地区痢疾杆菌耐药及质粒谱分布情况.方法 对分离自医院的66株痢疾杆菌流行株应用纸片扩散法测定细菌对13种抗生素敏感性,并采用Kado法进行质粒抽提.结果 红河地区痢疾杆菌耐药情况严重(100%),多重耐药普遍(9545%),耐药谱型分散,计有36种耐药谱型;共检出8种不同分子量的质粒,350Md和120Md单质粒检出率较高,分别为4242%和4545%;质粒谱型有12种,但以350Md和120Md单质粒谱型为主,合计占6818%.结论 将耐药谱型与质粒谱型进行比较,具有相同质粒谱型的菌株,其耐药谱型可相同,亦可不同,两者关系有待进一步探讨.结合文献报道,估计120Md是痢疾菌毒力质粒,并推测350Md是否为本地区的R质粒     

重型肝炎患者苏云金杆菌的耐药机制研究

目的 研究重型肝炎患者苏云金杆菌(Bt)的耐药机制.方法 应用美国BD公司的Phoenix NMIC/ID-55鉴定/药敏板进行鉴定与细菌药敏试验,应用PCR法检测Bt的耐药基因,并经测序及同源性分析证实其分布.结果 该株病原菌经16S rRNA测序及同源性分析(GenBank注册号为FJ932761)证实为Bt;对氨苄西林、万古霉素、替考拉宁、环丙沙星、呋喃妥因、四环素、夫西地酸、利奈唑胺敏感,对青霉素、苯唑西林、红霉素、克林霉素、喹奴普汀/达福普汀、庆大霉素、妥布霉素、阿米卡星、磺胺甲噁唑/甲氧苄啶、头孢西丁、莫匹罗星等药耐药;PCR扩增1种AMEs耐药基因阳性,经测序和同源性分析证实为ant(3")-Ⅰ,于GenBank注册号为FJ644661.结论 Bt对氨基糖苷类耐药机制主要与ant(3")-Ⅰ基因有关.     

马尾近端SPR的临床应用解剖学

目的：为限制性椎板切除的ＳＰＲ提供解剖学依据。方法：在２０例成人防腐灌注红色乳胶的脊柱标本上，对近端马尾神经进行巨—微解剖学观察。结果：马尾近端，Ｌ１前后根之间有最下一对齿状韧带，前根位于相应后根的前内侧。各节段后根在马尾近端呈冠状位的内外平排，从内向外依次为Ｓ２～Ｌ１的后根；各节段后根进脊髓处，Ｌ５和Ｓ１后根之间有明显分界线，相邻节段后根之间，均有恒定小静脉分隔。以终丝起点为定点，各节段后根与终丝之间的距离和成角从Ｓ２到Ｌ１逐渐增加。结论：在限制性椎板切除的ＳＰＲ术中，前、后根的鉴别主要依靠脊髓的外侧索和Ｌ１前后根之间的最下一对齿状韧带；后根节段的鉴别主要依靠后根连脊髓处，Ｌ５和Ｓ１之间有明显分界、相邻节段后根间有恒定小静脉来定性鉴别。也可根据终丝起点水平，各节段后根与终丝之间的距离和成角来鉴别后根节段     

Strategies for the analysis of osteitic bone defects at the diaphysis of long bones

Septic diseases of the bone and the immediate surrounding soft tissue, i.e., osteitis, belong to the most alarming findings in recent traumatology and orthopedic surgery. The paramount goal of this therapy is to preserve the stable weight-bearing bones while maintaining a correct axis and proper working muscles and joints, in order to avoid permanent disability in the patient. “State-of-the-art” therapy of osteitis/osteomyelitis therapy has two priorities: eradication of the infection and reconstruction of bone and soft tissue. Surgical treatment of the affected bone segments and soft tissue, followed by reconstructive methods, continues to be the main basic therapy. It is often extremely difficult to decide whether the affected bone segment has to be resected, or whether bone continuity can be preserved. The following paper provides strategies and guidance to help guide decisions in this complex and challenging area.     

Ss-A/Ro核蛋白60 ku亚单位及其变异体在耐药胃癌细胞中的表达

目的:克隆Ss-A／Ro 60 ku亚单位编码基因,检测Ss-A／Ro 60 ku亚单位及其变异体在耐药胃癌细胞中的表达情况．方法:应用RT-PCR克隆Ss-A／Ro 60 ku亚单位编码基因,应用半定量RT-PCR检测Ss-A／Ro 60 ku亚单位及其变异体在胃癌细胞中的表达情况．结果:成功克隆了Ss-A／Ro 60 ku亚单位编码基因,并发现了他的两种变异体,长度分别为52 bp和41 bp．半定量RT- PCR结果显示,Ss-A／Ro 60 ku亚单位在SGC7901／VCR中的表达强度高于SGC7901细胞(P=0．0001),Ss-A／Ro 60 ku亚单位的两种变异体在SGC7901／VCR中的表达强度也高于 SGC7901细胞(P=0．001)．结论:Ss-A／Ro 60 ku亚单位及其两种变异体为胃癌多药耐药相关分子．     

胰腺节段性切除治疗胰腺头颈部疾病的评价

目的 探讨胰腺节段性切除术治疗胰腺头颈部良性占位性病变的可行性和实用性。方法对2004年-2008年收治的15例胰腺头预部良性占位性病变的患者，实施了节段性胰腺切除，对该部位病变手术方式选择、手术步骤、手术要点、术后并发症和手术对患者胰腺内外分泌功能的影响进行分析。结果15例患者被实施了不同侧重选择的节段性胰腺切除术，其中胰腺颈部和部分胰头切除术6例，胰腺颈部切除术5例，胰颈和部分胰体部切除术4例。肿瘤病理分别是粘液性囊腺瘤、浆液性囊腺瘤、单纯囊肿、神经内分泌肿瘤和导管内乳头状粘液瘤。手术并发症发生率为20％，无手术死产，患者术后1周，血糖值与术前比较差异无统计学意义，术后检查粪便无脂肪微粒。结论节段性胰腺切除是胰腺头预部良性占位性病变较好的手术方式。     

复方玄驹口服液对性腺内分泌系统抗衰老生物效应的临床研究

对100例中老年性功能低下者,350例阳萎、早泄患者及30例健康成年男子服用复方玄驹口服液前后的血清性激素水平、脂质过氧化产物及红细胞超氧化物歧化酶活性进行测定。结果表明,服用以野生大黑蚂蚁(玄驹)为主药,佐以滋阴壮阳中草药制成的复方玄驹口服液1个月后,被试者精力、体力、睡眠等健康状况明显改善,对中老年性功能低下与性功能障碍的有效率分别达78％与71.14％,且使血清睾酮与SOD活性提高,使LH、E_2/T及血清MDA水平降低。故认为复方蚁制剂可通过调节性腺内分泌系统及抗脂质过氧化反应而起到抗衰老及治疗肾虚型性功能障碍的作用,并认为其药理作用与蚂蚁体内富含微量元素Mn、Zn、Se及维生素E有关。     

Exérèse des nævus congénitaux géants : jusqu’où aller avec la chirurgie ?

Surgical excision of giant congenital nevi is recommended by principle for dermatological reasons. Malignant potential is real but its incidence remains widely discussed. Their excision represents a surgical challenge but is also a real assault course for the child and his family. The sequelae and the psychological effects can be important. Can an incomplete excision to limit these aesthetic after-effects and relieve the surgical treatment be acceptable? We present the case of a child affected by a giant congenital nevi of the cephalic extremity where the excision was partial. A review of the literature on the degenerative risk of the giant congenital nevi allowed us of noticed that this one tends to be overestimated. The advantages and the disadvantages to practise a preventive, premature excision and complete of the giant congenital nevi are approached. We discuss the possibility to resort to a partial excision in certain cases delicate of reconstruction under the cover of a strict and moved closer dermatological surveillance.     

Terminology of Peters’ anomaly variants: Summary of histopathological findings in 6 corneas and detailed clinicopathological correlation in 2 cases

Peters’ anomaly accounts for the highest type of Anterior Segment Dysgenesis (ASD). The main features of Peters’ anomaly are: congenital corneal opacity centrally, defect in the posterior stroma and absence of Descemet’s membrane and the endothelium. However, this condition has wide clinical and histopathological variations in appearance, associations and severity. In this case series, we summarize 6 corneas in 5 Saudi cases of Peters’ anomaly (and describe 2 in detail) with unique histopathological findings that are additional to the typical known ones, shedding some light on the nomenclature of these variants according to the reported cases in the English-written literature. This will widen the spectrum of findings known to ophthalmic pathologists and ophthalmologists about this anomaly. This is also of importance in the assessment of the congenital glaucoma cases commonly seen in Saudi Arabia that often happens in association with ASD.