氩激光治疗视网膜静脉阻塞新生血管的临床研究

为探讨、分析氩激光治疗视网膜阻塞新生血管的疗效。氩激光治疗视网膜静脉塞新生血管５５例,中央静脉塞行全视网膜光凝。分支静脉阻塞区域播散性光凝,光斑２００－５００μｍ,时间０．１－０．２ｓ,能量０．１２－０．４ｗ,Ⅱ级光斑,蓝绿色光波,黄斑格栅光凝光斑１００μｍ,时间０．１ｓ,能量视病情而定,以Ⅰ级反应为度,绿色光波。结果：５５例中４４例有效,新生儿血管消嫁总有效率８０％,无效１１例（其中视盘有新生血     

视网膜光化学损伤大鼠视紫红质基因mRNA表达水平的原位杂交技术检测

目的：研究视网膜光化学损伤状况下视紫红质基因mRNA表达水平的变化规律及其与形态学改变的关系。 方法：应用原位杂交和电镜技术对光化学损伤大鼠视网膜视紫红质基因mRNA表达情况及视网膜形态学损伤性改变进行动态观察。 结果：视紫红质mRNA原位杂交信号主要分布于视网膜光感受细胞层,尤其是其内段和外段;随连续光照时间的延续,其表达迅速下降,且先于视网膜形态学的损伤性改变;同一视网膜中,上方及后极部杂交信号的减弱较下方和周边部更为明显。 结论：视紫红质基因mRNA表达水平降低可能为光化学性视网膜损伤的早期信号。 （中华眼底病杂志,1997,13：228-230）     

Effects of prolonged uniocular dark adaptation on the direct-current electroretinogram of pigmented and albino rabbits

The direct-current electroretinogram of seven pigmented and seven albino rabbits was recorded from both eyes for almost 4 h in response to repeated identical light stimuli. Stimulus duration was 10 s, light intensity was 6.8 × 10² lux, and the interval between the beginning of succeeding light stimuli was 3 min. The dark-adaptation period preceding light stimulation was 30 min for one of the eyes (unoccluded eye) and 150 min for the contralateral eye (occluded eye), which was patched during the first part (117 min) of the experiment. In pigmented animals, the b- and c-wave amplitudes of the unoccluded eye slowly increased during the first part of the experiment but not significantly during the second. The a-wave amplitude was not significantly changed. After removal of the cover, the a- and b-wave amplitudes of the occluded eye immediately attained but did not exceed the level of those in the unoccluded eye, irrespective of the light adaptation induced by the stimulus flashes previously presented to the unoccluded eye. (Control experiments on six pigmented rabbits confirmed that stimuli identical to those used in the main part of the study caused a light adaptation, since a decrease in a- and b-wave amplitudes occurred after the first light stimulus following an initial dark-adaptation period of 2 h for both eyes.) In albino rabbits, electroretinogram responses were clearly discernible in the occluded eye also during the first part of the experiment, probably because of transillumination of the head. In other respects, the results were essentially similar to those of pigmented animals. The observation that occluded eyes did not dark adapt better, as judged by the electroretinogram responses, than contralateral eyes given repeated light adaptive stimuli may indicate the presence of a mechanism for transfer of adaptation information between the eyes.     

A cyclic adenosine monophosphate agonist elevates the b- and c-waves of the rabbit direct-current electroretinogram

The effects of the stable cyclic adenosine monophosphate analogue adenosine 3, 5-cyclic monophosphorothioate Sp-isomer (Sp-cAMPS) on the direct-current electroretinogram and the standing potential of the eye were studied. Corneal recordings were obtained from unilaterally vitrectomized albino rabbit eyes during alternating intravitreal perfusions with Sp-cAMPS and a control solution (Pharmacia eye irrigating solution). The contralateral eye was used as a control. To evaluate further the effects on the c-wave,in vivo intraretinal microelectrode measurements were made during simultaneous intravitreal perfusion of Sp-cAMPS and irrigating solution, respectively. Sp-cAMPS in concentrations of 1, 10 and 100µM was tested by corneal direct-current electroretinography. There was no significant effect on the a-wave amplitude. The b-wave amplitude was reversibly elevated at an Sp-cAMPS concentration of 100µM (p<0.01, n=7). The c-wave amplitude was reversibly elevated at a concentration of 10µM (p<0.001, n=8), and this effect was more pronounced at 100µM (p<0.001, n=7). The SP increased reversibly at a concentration of 100µM (p<0.001, n=7). Microelectrode recordings were performed with Sp-cAMPS at a concentration of 100µM. The recordings showed significant increases in both the transepithelial potential (p<0.01, n=3) and the slow PIII (p<0.01, n=3). The effects of Sp-cAMPS on the b-wave as well as on the two components of the c-wave suggest influences on both the inner retina and the retinal pigment epithelium of the rabbit eye.Abbreviations PHS Pharmacia eye irrigating solution - AMP adenosine monophosphate - Sp-cAMPS adenosine 3, 5 - cyclic monophosphorothioate Sp-isomer     

Laser Doppler velocimetry in retinal arteries of infants

The present study was conducted to assess the feasibility of laser Doppler velocimetry in young infants, as a prelude to ultimately undertaking such measurements in premature infants. A portable, unidirectional laser Doppler velocimeter was developed based on a Kowa RC-2 hand-held fundus camera. Six infants between 1 and 21 weeks of age were studied. Relative red blood cell velocity (f_max) at the centre of retinal arteries was measured over approximately 10 heart cycles. A pulsatility parameter (P=1–f_max.dia/f_max.sys), a summary index of vascular status, was determined from the average diastolic and systolic values of f_max. Velocity waveforms were obtained in four of the six infants. Arterial pulsatility for the group was 0.63±0.13. Precise non-invasive measurement of arterial red blood cell velocity waveforms in young infants was achieved. The high signal-to-noise ratio and temporal resolution of this data suggest that relative measurements of retinal blood flow may permit assessment of haemodynamic changes in premature infants.     

视细胞移植术后视网膜色素上皮的结构变化

获得纯化的由大肠杆菌表达的小鼠 Ｆａｓ配体（Ｆａｓ ｌｉｇａｎｄ,ＦａｓＬ）。方法：以质粒 ｐＥＴ－１５ｂ为载体,在大肠杆菌ＢＬ２１（ＤＥ３）中表达小鼠的ＦａｓＬ,用金属螫合亲和层析法纯化。结果：转化菌中,重组质粒是稳定的,异丙基－β－Ｄ硫代半乳糖苷可诱导小鼠ＦａｓＬ的表达 ,金属螫合亲和层析法可以初步纯化此蛋白。结论：在大肠杆菌中表达出小鼠的ＦａｓＬ,并得到初步纯化。     

图形视网膜电图检测原发性开角型青光眼视网膜功能的意义

目的 观察原发性开角型青光眼图形视网膜电图的改变及其特点,了解ＰＥＲＧ在检测ＰＯＡＧＰＹ视网膜功能方面的意义。方法 对３６例（５９只眼）ＰＯＡＧ及３２例（５９只眼）年龄相匹配的正常人进行ＰＥＢＧ检测。结果 ＰＯＡＧ患者ＰＥＲＧ的ＡＰ１、ＡＮ２、ＡＮ１、ＡＮ２／ＡＰ１下降;运用ＡＮ２＋ＡＰ１〈２．７及ＡＮ２／ＡＰ１下降;运用ＡＮ２＋ＡＰ１〈２．７及ＡＮ２／ＡＰ１〈０．７的方法,对已确认的ＰＯＡＧ进行     

视网膜脱离巩膜环扎术后眼前节结构的初步观察

目的 观察巩膜环扎术后眼前节结构的变化,探讨术后继发性闭角型青光眼的发病机制。方法 应用超声生物显微镜检查３０例（３３只眼）孔源视网膜脱离患者术前、后３－７天的活体奶前节形态结构,并分别进行定量测定,检查结果经统计学分析两组间配对ｔｓｗｃｗ,以Ｐ〈０．０５作为差异有显著性标准。结果 术前１只眼存在裂隙样睫状体脱离。术后１１只眼（３３．３％）发生睫状体脱离,睫状体增厚水肿;术前睫状体厚度为（８２１．     

硅油下视网膜复位术疗效探讨

目的 总结硅油填充术后视网膜再脱离行硅油正视网膜复位术的疗效,探讨该手术的适应证及手术技巧。方法 对１７例（１７只眼）硅油填充术后视网膜再脱离者,在保留硅油的条件下,做睫状体扁平部３切口,在导光纤维的导引下行剥膜、断膜、视网膜切开、内放液、内激光光凝、视网膜下全氟化碳液体取出、补充硅油等使视网膜得位。结果 １７只眼中,术后视网膜重位１４只眼,成功率为８２．４％。术中硅油误入前房１只眼,误入脉络膜上