新型过氧乙酸溶液应用于消化内镜消毒50例

目的:探讨新型过氧乙酸溶液应用于消化内镜消毒的临床效果。方法:消化内镜100例,随机分成对照组与观察组,每组50例。对照组应用2%戊二醛溶液,观察组应用新型过氧乙酸溶液。结果:观察组总合格率100%与对照组96.00%相比,差异无统计学意义(P>0.05)。结论:新型过氧乙酸溶液应用于消化内镜消毒效果比较可靠,且对内镜无损伤,具有良好的安全性。     

牛颈静脉带瓣管道的抗钙化研究进展

应用带瓣管道重建右心室流出道是治疗复杂先天性心脏病的一项重要手段。近年来,戊二醛处理的牛颈静脉带瓣管道被广泛应用于临床,但术后管道钙化、衰败问题一直未得到解决。目前抗钙化处理方法有去细胞、染料介导光氧化、环氧化合物、组织工程等,但单一方法往往不能取得令人满意的效果,多种方法联合处理将是组织工程技术发展的方向。     

Meniscal material properties are minimally affected by matrix stabilization using glutaraldehyde and glycation with ribose.

Knee meniscus replacement holds promise, but current allografts are susceptible to biodegradation. Matrix stabilization with glutaraldehyde, a crosslinking agent used clinically to fabricate cardiovascular bioprostheses, or with glycation, a process of crosslinking collagen with sugars such as ribose, is a potential means of rendering tissue resistant to such degradation. However, stabilization should not significantly alter meniscal material properties, which could disturb normal function in the knee. Our objective was to evaluate the effects of glutaraldehyde- and glycation-induced matrix stabilization on the material properties of porcine meniscus. Normal untreated meniscus specimens were tested in confined compression at one of three applied stresses (0.069, 0.208, 0.347 MPa), subjected to either a glutaraldehyde or glycation stabilization treatment, and then re-tested to measure changes in tissue aggregate modulus, permeability, and compressive strain at equilibrium. Changes in these properties significantly increased with glutaraldehyde concentration and exposure time to ribose. One glutaraldehyde and three glycation treatments did not alter aggregate modulus or compressive strain at equilibrium compared to controls (p > 0.10). However, all treatments increased permeability by at least 108% compared to controls (p < 0.001). This study reveals a dose-dependent relationship between meniscal material properties and certain stabilization conditions and identifies treatments that minimally affect these properties. Further research is necessary to determine whether these treatments prevent enzymatic degradation before and after surgical implantation in the knee.     

表面活性剂对戊二醛消毒液稳定性的研究

目的：研究寻找一种使2%戊二醛消毒液既无腐蚀性又稳定的试剂。方法：用0.25%非离子型表面活性剂苄泽35或OP-10替代3%NaHCO3。对取代后的2%戊二醛消毒液采用恒温法在40℃，60℃及80℃进行稳定性试验，留样观察及其对医疗器械的腐蚀性考察。含量测定方法按照《中国医院制剂规范》第1版的方法进行测定。结果：用苄泽35取代后的2%戊二醛消毒液在稳定性试验及留样观察中，消耗H2SO4滴定液（0.05000mol/L）的毫升数仍在限度：3.6-4.4ml之间；浸包5个月后的医疗器械光滑，无斑点。结论：取代后的2%戊二醛消毒液的稳定性高，对医疗器械无腐蚀。     

口腔正畸科、修复科交叉感染的预防研究

因印模等直接与病人唾液、血液、食物残渣接触,成为重要疾病传染源之一。本实验研究紫外灯照射模型、2％戊二醛浸泡印模和(牙合)蜡的消毒效果,结果提示两者均能快速有效地杀灭细菌,切断通过印模等传播疾病的途径。     

Banana peel wastes as a source of tyrosinase useful in the production of l-DOPA

Tyrosinase from the banana peel (TBp) served as a low-cost immobilized biocatalyst for the production of 3, 4-di hydroxyphenyl-l-alanine (l-DOPA). l-DOPA, a dopamine precursor, useful in the management of Parkinson's disease, was produced in improvised reactors. l-DOPA production by the immobilized TBp was optimized via response surface methodology (RSM). l-DOPA productivities obtained at equimolar substrate concentrations and 500 mg of TBp for the packed bed and batch process (BP) were 34.0 ± 2.1 and 15.4 ± 0.9 mg/L/h respectively. The resulting l-DOPA productivity in the BP after the RSM model validation was 63 ± 3.0 mg/L/h. There was a good fit with the model obtained based on the closeness of the predicted and experimental values. After four successive batch processes, about 35% loss in l-DOPA productivity was observed. In conclusion, banana peel served as a relatively cheap and easily available source of tyrosinase for the production of l-DOPA in both batch and packed bed reactors.     

Biochemical differences between dystrophic calcification of cross-linked collagen implants and mineralization during bone induction

Summary Ectopic calcification of diseased tissues or around prosthetic implants can lead to serious disability. Therefore, calcification of implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen was compared with mineralization induced by demineralized bone matrix (DBM). Whereas implants of DBM accumulated large amounts of calcium and a bone-specific γ-carboxyglutamic acid protein (BGP or osteocalcin) following implantation in both young and older rats, implants of cross-linked pericardium calcified with only traces of BGP. Glutaraldehyde-cross-linked DBM failed to calcify after implantation in 8-month-old rats for 2–16 weeks. Implants of cross-linked type I collagen exhibited small calcific deposits 2 weeks postimplantation but calcium content eventually dropped to levels equal to those of soft tissues as the implants were resorbed. The calcium content of DBM implanted in 1- and 8-month-old rats reached comparable levels after 4 weeks, but the BGP content was approximately twice as high in the younger animals than in the older ones. Glutaraldehyde-cross-linked implants of DBM, tendon, and cartilage calcified significantly in young but not in old animals. This form of dystrophic calcification was associated with only trace amounts of BGP. Alkaline phosphatase activity was high in implants of DBM and undetectable in implants of cross-linked collagenous tissues. These results show that implants of glutaraldehyde-cross-linked collagenous tissues and reconstituted collagen calcify to different extents depending upon their origin and the age of the host, and that the mechanism of dystrophic calcification differs significantly from the process of mineralization associated with bone induction as reflected by alkaline phosphatase activity and BGP accumulation.     

Effect of 0.5% glutaraldehyde disinfection on surface wettability of elastomeric impression materials

Purpose

The purpose of this in-vitro study was to evaluate the effect of 0.5% glutaraldehyde spray disinfection on surface wettability of vinyl polysiloxane and polyether impression materials.

含氟烷人血白蛋白靶向超声造影剂的制备研究

目的为靶向超声造影剂的制备进行基础实验研究。方法将含氟烷人血白蛋白超声造影剂与ICAM-1抗体混合,加入0.1%戊二醛,4℃孵育2h,促使抗体与微泡外壳充分交联。普通光镜评估靶向微泡的大小、形态。免疫荧光法进行靶向微泡的鉴定。结果普通光镜下可见携ICAM-1抗体的靶向微泡大小、形态与普通白蛋白微泡对照无明显改变。荧光显微镜证实微泡与ICAM-1抗体整合成功。结论戊二醛交联法适用于白蛋白靶向超声造影剂的制备,方法简便,成本低,重复性好。     

邻苯二甲醛与戊二醛在消化内镜消毒中的比较研究：系统评价

目的：对邻苯二甲醛在消化内镜消毒应用中的消毒效果、经济学指标、安全性进行系统评价。方法：计算机检索中国生物医学文献服务系统、中国知网资源总库（CNKI）、万方数据知识服务平台、维普期刊资源整合服务平台(VIP),纳入国内邻苯二甲醛对比戊二醛在消化内镜消毒效果、经济学指标、安全性三方面的对照研究,检索时间截至2016年7月。对研究结果进行定性分析。结果：最终纳入14项对照研究,但存在较大的异质性。定性分析结果显示：12项研究的消毒效果对比结果显示邻苯二甲醛浸泡5分钟与戊二醛浸泡≥10分钟无差异,均可达到高水平消毒。9项研究的经济学指标对比结果显示邻苯二甲醛耐用性更好,消毒效率更高,经济效益更佳。并且邻苯二甲醛的安全性优于戊二醛。结论：邻苯二甲醛可替代戊二醛成为消毒消化内镜的首选高水平消毒剂。