检测禽流感病毒的压电免疫芯片两种抗体固定方法的研究

目的探讨构建检测禽流感病毒压电免疫芯片的2种抗体固定方法。方法以AT切型、基频10 MHz的石英晶体为材料,分别采用聚乙烯亚胺-戊二醛交联法(简称戊二醛法)、葡萄球菌A蛋白(SPA)法将禽流感病毒H5亚型单克隆抗体固定在石英晶体的金膜电极表面,构建检测禽流感病毒压电免疫芯片。结果戊二醛法构建的压电免疫芯片检测H5抗原的线性范围为稀释度1∶2~1∶40,Y=75.814-13.748X,r2=0.978 9,与H9抗原不存在交叉反应,与鸡胚分离法的符合率达到91.7%。SPA法若先固定抗体,则无法检测到抗原;如先进行抗原抗体反应再与SPA结合,可以达到定性检测的效果。结论戊二醛法适合于构建禽流感病毒压电免疫芯片,其检测线性满足检测要求;SPA法在检测抗原时存在局限性,尚不能直接检测病毒抗原。     

长效反定型红细胞试剂的研究

目的：通过研究实验探索最佳的醛化剂浓度及醛化时间对红细胞进行醛化处理,以获得保存效期长、凝集效果佳的反定型红细胞试剂。方法：分别以0.5%、0.1%、0.05%的戊二醛对洗涤后的红细胞进行1分钟、3分钟、5分钟、10分钟的醛化,配制成5-10%的醛化反定型红细胞试剂,分别与购买的商品化反定型红细胞试剂,单克隆抗A、抗B正定型试剂进行对比试验,同时在2-8℃冰箱放置进行稳定性试验。结果：用0.1%的戊二醛醛化红细胞可以获得最佳的稳定性与凝集效果的平衡,在12个月的保存试验期内试剂稳定,与商品化的反定型红细胞试剂及正定型的对比实验中,二者的结果完全相符。结论：0.1%的戊二醛醛化反定型红细胞试剂具有良好的稳定性及凝集效果,可以用于ABO血型的反定型,同时用醛化的方法制备稳定而又凝集效果佳红细胞试剂也是一条值得探索的途径。     

酸性氧化电位水消毒消化道内镜效果的观察

目的：观察酸性氧化电位水消毒消化内镜的效果。方法：设立酸性氧化电位水组与2％戊二醛两组,采用5槽消毒法。分别消毒3min、5min、10min,采样消毒前后的内镜标本进行细菌计数。结果：酸性氧化电位水消毒5min和10min时菌落数均为0．优于戊二醛组。结论：酸性氧化电位水可以提高消化道内镜的消毒效果。     

戊二醛及其强化制剂对枯草杆菌黑色变种芽孢杀灭效果的实验研究

①目的 探讨不同类型戊二醛消毒剂的杀芽孢效果。②方法 采用定量杀菌实验，计算出其杀菌率。③结果 强化中性戊二醛、强化碱性戊二醛、强化酸性戊二醛消毒剂都含有强化剂，2％的上述3种消毒剂对枯草杆菌黑色变种芽孢作用4小时，对枯草杆菌黑色变种芽孢的杀灭率达100％，而2％普通戊二醛对枯草杆菌黑色变种芽孢的杀灭作用相对较弱，尤其在有机物存在的条件下，普通戊二醛对枯草杆菌黑色变种芽孢的杀灭作用较强化的戊二醛明显偏弱。④结论 强化戊二醛的杀灭效果强于普通戊二醛。     

微软酸化水对胃镜消毒的临床研究

目的:探讨超微软酸化水四槽法和2%戊二醛四槽法对胃镜消毒效果、消毒时间及其安全性。方法:选取2000年6月至2003年7月使用戊二醛与2003年8月至2007年6月使用超微软酸化水的情况进行总结比较。结果:胃镜用超微软酸化水浸泡消毒3min,无细菌生长,可杀灭乙肝表面病毒,而用2%戊二醛浸泡消毒则需10min,两种消毒剂的消毒效果相同。结论:胃镜消毒时使用超微软酸化水浸泡消毒3min可达到内镜消毒标准。超微软酸化水用于胃镜消毒具有良好的杀菌效果,其具有消毒时间短,效果好,无刺激性等特点。     

Triamcinolone-loaded glutaraldehyde cross-linked chitosan microspheres: Prolonged release approach for the treatment of rheumatoid arthritis

The use of glucocorticoids in the treatment of rheumatoid arthritis has been widely employed, but, owing to their systemic side-effects and also their susceptibility to the first pass metabolism, their use is being discouraged. To circumvent this, triamcinolone (TA) were encapsulated in chitosan microspheres with glutaraldehyde as the cross-linking agent to achieve a prolonged drug release. The percentage of drug loading, encapsulation efficiency, and surface morphology by Scanning electron microscopy (SEM), Phase transition by Differential scanning colorimetry (DSC), as well as Fourier transform infrared spectroscopy (FTIR) studies was carried out to characterize the chitosan microspheres. In-vitro and in-vivo release studies revealed that microspheres were able to control the release of TA with a uniform release pattern up to a period of 36 days and thereafter an extended release up to 63 days. The clinical parameters were investigated for changes in paw volume, hematological parameters like Erythrocyte sedimentation rate (ESR), Paced cell volume (PCV), Total leucocyte count (TLC), Hb, and Differential cell count (DCC) in Fruend’s complete adjuvant induced arthritic rats. Histopathological findings as well as radiology (X-ray) further confirmed the effectiveness of TA encapsulated microspheres in mitigating the rat arthritic model.     

Do collagen cross-linkers improve dentin’s bonding receptiveness?

Objectives

Dentin biomodification using collagen cross-linkers has been proposed as one of the strategies to improve bond durability of adhesives to dentin. However, literature is not very consistent regarding their benefit, in particular when cross-linkers are applied in clinically realistic application times. This study investigated the effect of three cross-linkers on the mini-interfacial fracture toughness (mini-iFT) of four adhesives bonded to dentin following either etch&rinse (E&R) or self-etch (SE) modes.

0.5%戊二醛溶液含量测定影响因素的考察

目的：考察测定0.5%戊二醛溶液含量的影响因素。方法：采用酸碱滴定法检测0.5%戊二醛溶液不同取样量、不同稀释度及不同pH值对戊二醛测定准确度的影响。结果：不同取样量、稀释度对测定结果均有不同程度的影响,而不同pH值对测定结果有显著性影响。结论：测定5%戊二醛溶液含量取量0.100～0.200g较适宜;溶液在pH〉8时,溶液稳定性极差。     

不耐高温手术器械两种灭菌方法的效果比较

目的探讨不耐高温手术器械寿命的灭菌方法。方法对过氧化氢等离子低温灭菌法和戊二醛气体熏蒸灭菌箱消毒不耐高温手术器械进行比较。结果两种灭菌方法均达到灭菌要求;过氧化氢等离子低温灭菌法比戊二醛气体熏蒸灭菌箱灭菌法省时155min;灭菌相关器械损耗率分别为戊二醛气体熏蒸灭菌箱0．18％,过氧化氢等离子低温灭菌法为0,差异有统计学意义（P〈0．01）;灭菌剂毒性反应发生率分别为戊二醛气体熏蒸灭菌箱灭菌法3．24％,过氧化氢等离子低温灭菌法为0,差异有统计学意义（P〈0．05）。结论使用过氧化氢等离子低温灭菌器消毒不耐高温手术器械,灭菌周期短,质量可靠,对器械损伤小,对人和环境无害,是不耐高温手术器械首选的灭菌方法。     

Inhibition of endogenous human dentin MMPs by Gluma

ObjectiveThe objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight.MethodsDentin beams of 2 mm × 1 mm × 6 mm were obtained from extracted human third molars coronal dentin. To measure the influence of Gluma treatment time on total MMP activity of dentin, beams were dipped in 37% phosphoric acid (PA) for 15 s and rinsed in water. The acid-etched beams were then dipped in Gluma for 5, 15, 30 or 60 s, rinsed in water and incubated into SensoLyte generic MMP substrate (AnaSpec, Inc.) for 60 min. Controls were dipped in water for 60 s. Additional beams of 1 mm × 1 mm × 6 mm were completely demineralized in 37% PA for 18 h, rinsed and used to evaluate changes on the dry weight and modulus of elasticity (E) after 60 s of Gluma treatment followed by incubation in simulated body fluid buffer for 0, 1 or 4 weeks. E was measured by 3-pt flexure.ResultsGluma treatment inhibited total MMP activity of acid-etched dentin by 44, 50, 84, 86% after 5, 15, 30 or 60 s of exposure, respectively. All completely demineralized dentin beams lost stiffness after 1 and 4 weeks, with no significant differences between the control and Gluma-treated dentin. Gluma treatment for 60 s yielded significantly less dry mass loss than the control after 4 weeks.SignificanceThe use of Gluma may contribute to the preservation of adhesive interfaces by its cross-linking and inhibitory properties of endogenous dentin MMPs.