Ⅳ型胶原和透明质酸测定早期肝纤维化的临床价值

肝纤维化是一切恶性肝病的共同病理学基础,是肝硬变的初级阶段,并与肝癌的发生有一定关系,肝纤维化是细胞外基质,合成和降解失衡的结果,在进入肝硬变之前尚有逆转的可能。Ⅳ型胶原和透明质酸是细胞外基质的主要成分,作为检测肝纤维化的指标[1]。我科通过检测56例健康人和108例肝病患者血清HA和IV-C的含量,并探讨其临床价值。     

大鼠脂肪干细胞复合胶原-壳聚糖-硫酸软骨素三维支架构建组织工程软骨

Objective To evaluate the character of the collagen-chitosan-chondroitin sulfate scaffold seeded with rat adipose tissue-derived stromal cells. Methods A dipose tissue were harvested from 6 weeks old Wistar rats and the stromal cells were harvested by type Ⅰ collagenase and then cultured in vitro. Type Ⅰ collagen was fully mixed with chitosan, freeze-dried and cross-linked with chondroitin sulfate, then freeze-dried again and sterilized by ethylene oxide. The pore diameter, water content, porosity of the scaffold were tested. The adipose tissue-derived stromal cells were digested, seeded into the plates, scaffold, and cen-trifuged into pellet, and then induced into cartilage. MTT detection for cell proliferation was done. After 3 weeks, the cell morphology, and cell proliferation and adhesion were observed, and chondrngenic differenti-ation was also analyzed. Results The pore diameter, water content, porosity tested for the scaffold showed an appropriate form. Cell proliferation showed faster in the scaffold and pellet culture system after 5 day, there was still cell proliferation in the scaffold system after 14 days but no obvious changes in the pellet cul-ture system; ceils on the scaffold proliferated densely showed by histological staining, but there was a scaf-fold structure residues in the inner layer. The finding of type Ⅱ immunohistochemistry stain showed that cells express strong positive for type Ⅱ collagen in the scaffold and pellet culture system whereas it was weakly positive in the plate culture system; the specific mRNA for cartilage, type Ⅱ collagen, aggrecan and SOX-9 were expressed in all three systems showed by RT-PCR, but type X collagen was expressed continu-ously in the plate culture system and expressed after 21 days in the pellet culture system, whereas it was not detected in the collagen-chitosan-chondroitin sulfate scaffold system. Conclusion The parameters of the collagen-chitosan-chondroitin sulfate scaffold were suitable in our study. The results suggested that it can promote the adipose tissue-derived stromal cells proliferation and chondrogenic differentiation better than the plate and pellet culture systems and maintain the phenotype of chondrocytes well; it is the optimal choice for cartilage tissue engineering in the future.     

氧氟沙星壳聚糖胶囊的稳定性研究

氧氟沙星壳聚糖胶囊是以氧氟沙星、壳聚糖、赤石脂等为原料,按现代药剂学工艺制备而成的胶囊剂.为了考察光照、高温、高湿度、低温、室温留样、加速实验等因素对氧氟沙星壳聚糖胶囊的影响,按新药审批办法中有关规定对其稳定性进行了试验研究[1],报道如下.     

粉防己碱和氯丙嗪对人胚肺成纤维细胞胶原和透明质酸合成的影响

研究粉防己碱和氯丙嗪胚肺成纤维细胞胶原与透明质酸合成的影响，为应用钙拮抗剂治器官纤维化提供依据。采用［＾３Ｈ］脯氨酸掺入和放射免疫法分别测定胶原与ＨＡ合成。结果：Ｔｅｔ５－８０μｍｏｌＬ＾－１和Ｃｈｌ１０－４０μｍｏｌＬ＾－１均以浓度依赖方式抑制胶原与ＨＡ合成。Ｔｅｔ５－２０μｍｏｌＬ＾－１对细胞无明显毒性却显著抑制胶厚与ＨＡ合成。     

抗Ⅲ型前胶原肽单克隆抗体的制备及初步应用

制备出两株分泌抗ＰⅢＰ（Ⅲ型前胶原肽）单克隆抗体的杂交瘤细胞株。竞争抑制ＥＬＩＳＡ试验表明：两株单抗与ＰＣＩ（Ⅰ型前胶原）及ＣⅢ（Ⅲ型胶原）无交叉反应。Ｄｏｔ－ＥＬＩＳＡ示两株单抗钧针对ＰⅢＰ构象决定簇。应用所制备的单抗对人肝细胞癌组织及大鼠、小鼠成纤维母细胞进行免疫定位。结果显示两种成纤维母细胞呈明显膜阳性；肝癌细胞浆内呈粗颗粒状染色，提示肝癌细胞具有合成Ⅲ型前胶原的能力。     

胶原植入与美容

胶原来源及成份本品从小牛皮肤提炼制成，是一种半透明的物质，含Ⅰ型胶原95～98％，其余是Ⅱ型胶原，1ml制剂中含胶原35mg，另有磷酸盐缓冲液和利多卡因。冷藏保存。由四川省中药研究所提供。临床资料一、观察对象：本组病例21人，女11人，男10人，年龄最大48岁，最小7岁，平均21岁。痤疮后遗疤痕14例，老年性皱纹2例，外伤后遗疤痕3例，脓疱疮，疖后遗疤痕2例。其中20例未作过任何治疗，一例因外伤后遗疤痕于1年前作过磨削术，疗效不满意。     

糖尿病肾脏胶原非酶糖化与氨基胍阿斯匹林的治疗作用

本实验系统观察了ＳＴＺ所致糖尿病鼠在１、３、６个月后肾脏组织胶原发生非酶糖化的情况，及氨基胍、阿斯匹林对肾脏胶原非酶糖化的阻断作用。结果表明：各病程糖尿病动物肾组织的胶原含量和非酶糖化早期产物（５－ＨＭＦ）及胶原相联荧光值均明显高于正常对照。氨基胍能够减弱糖尿病动物肾组织胶原含量的增加和荧光产物的生成，但对５－ＨＭＦ含量无影响。阿斯匹林未显示出对胶原非酶糖化的影响。     

壳聚糖与疫苗的黏膜递呈

具有良好生物相容性及生物降解性的壳聚糖已用于眼、口、鼻和生殖道黏膜的药物递呈以及DNA转染的研究。壳聚糖成本低，很容易制备成微粒，加栽容量大，具有与蛋白和DNA结合率高的特点，是疫苗黏膜递呈的首选对象。先进的微粒药物递呈系统的研究为壳聚糖用于抗原和药物的黏膜递呈开创了新思路，壳聚糖及其相关的最新研究在口腔防龋DNA疫苗和口腔药物递呈中有广阔的应用前景。本文就壳聚糖的特性、微粒的制备、黏膜递呈和免疫佐剂作用进行综述。     

慢性肝病患者血清TGFβ1,I—C,Ⅲ—C水平及其与肝纤维化的关系

应用酶免疫技术对１１８例慢性肝炎，肝硬化患者进行血清转化生长因子β１（ＴＧＦβ１），Ⅰ，Ⅲ型胶原（Ⅰ－Ｃ，Ⅲ－Ｃ）含量测定，分析三者与肝功能指标间相关性，结果表明，患者血清ＴＧＦβ１，Ⅰ，Ⅲ型胶原含量均高于正常对照组，且随慢迁肝→慢活肝→肝硬化逐渐递增，组间差异显著，血清ＴＧＦβ１，与Ｉ－Ｃ，Ⅲ－Ｃ谷丙转氨酶呈正相关，与白／球比值，血清总胆红素不相关，提示ＴＧＦβ１可能是一种很有前途的肝纤维化血清     

肺癌患者血清Ⅳ型胶原水平测定的临床意义

目的探讨肺癌患者血清Ⅳ型胶原水平的临床意义.方法用放射免疫法测25例及76例肺癌患者血清Ⅳ型胶原水平.结果血清Ⅳ型胶原水平Ⅰ期患者与正常对照组无显著性差异(P＞0.05),但Ⅳ期患者＞Ⅲ期＞Ⅱ期＞Ⅰ期(P＜0.05或P＜0.01).结论血清Ⅳ型胶原水平可作为判断肺癌病情发展和预后的良好指标.