转基因抗黄瓜花叶病毒蕃茄安全性评价研究

对转基因抗黄瓜花叶病毒 (CMV)蕃茄进行了安全性评价。结果 :CMV蕃茄急性毒性半数致死量LD50 >1 0 g/kg ,属实际无急性毒性物质。经微核试验、精子畸变试验及Ames试验均未发现有致突变作用 ;3 0d喂养试验也未发现引起异常改变。提示 :在本实验范围内 (相当于人口服 5 0 0 g/d)食用CMV蕃茄是安全的     

Spectrophotometric determination of the tannin contents of various Turkish black tea,beer and wine samples

This study reports tannin contents of various tea (19), beer (6) and wine (6) samples, produced or sold in Turkey under different brand names. Determinations were carried out by employing a previously reported UV-vis spectrophotometric method. The standard addition procedure was also compared to the direct determination procedure and found to be more reliable. The tannin contents of hot water extracts of tea, tea bag and herbal tea samples ranged between 6.20-8.33, 8.03-6.59 and 2.76-6.54 percent (w/w) respectively. Tannin contents of beer and wine samples determined without extraction were found ranging between 66.36-77.26 and 67.18-107.62 μ g/mL respectively. Iron contents of analysed wine samples were also found ranging between 0.36 and 10.33 ppm by AAS and no relation is found between tannin and iron contents.     

河套灌区覆膜沟灌对加工番茄生长效应与品质的影响

针对传统灌溉中存在不足,在内蒙古河套灌区研究了沟灌覆膜和无覆膜形式对加工番茄植株生育指标、品质、水分生产率、地上植株干物质积累量和番茄产量以及经济效益的影响。结果表明:覆膜处理对沟灌措施下的番茄植株的株高相比无覆膜处理增加达43%,茎粗增加达45%,番茄红素分别增加6.5%～25.4%;水分生产率及地上植株干物质的积累也分别提高了56%和22%,植株产量增长了1848.15～2768.89 kg·667m^-2,农民也得到了692.75～1592.75元·667m^-2的增产收入。因此在沟灌加工番茄生产中采取覆膜种植能有效促进生长并能取得良好的经济效益。     

Effects of Aqueous Extract of Lycopersicum esculentum L. var. “Camone” Tomato on Blood Pressure,Behavior and Brain Susceptibility to Oxidative Stress in Spontaneously Hypertensive Rats

Behavioral disorders affect millions of people worldwide. Hypertension contributes to both the development and progression of brain damage and cognitive dysfunction and could represent the most powerful modifiable risk factor for cerebral vessel dysfunction and consequent behavioral impairment. Tomato contains antioxidants and bioactive molecules that might play an important role in the prevention of cardiovascular and brain diseases. The effects of the combined gel and serum from Lycopersicum esculentum L. var. “Camone” tomatoes and those of purified tomato glycoalkaloids (tomatine) and an antihypertensive drug (captopril) were investigated in male spontaneously hypertensive rats (SHRs) and compared with normotensive Wistar Kyoto (WKY) rats. Body weight, systolic blood pressure, behavioral parameters, as well as brain susceptibility to oxidative stress and brain cytokine contents, were assessed. Treating hypertensive rats with tomato gel/serum or captopril for four weeks caused a significant reduction in blood pressure, decreased locomotor activity and increased grooming behavior; the last two parameters were also significantly affected by tomatine treatment. Brain slices obtained from hypertensive rats treated with tomato gel/serum were more resistant to oxidative stress and contained lower levels of inflammatory cytokines than vehicle-treated ones. In contrast, tomatine treatment had no effect. In conclusion, the tomato-derived gel/serum can be considered a dietary supplement able to drive in vivo blood pressure towards healthier values and also control some central effects such as behavior and brain oxidative stress.     

艾灸神阙穴对不同程度力竭运动大鼠海马区单胺类神经递质的影响

目的:探讨艾灸神阙穴对不同程度力竭运动大鼠海马区单胺类神经递质的影响。方法:将72只SD雄性大鼠按随机数字表法分为正常组8只、模型组和艾灸组各32只,根据造模或治疗时间将模型组和艾灸组随机分为1、4、7、10次亚组,每亚组8只,采用游泳实验建立力竭模型。艾灸各亚组大鼠造模后即刻温和灸神阙穴15min,隔日1次,各亚组大鼠完成相应力竭运动后24 h检测海马组织5-HT、DA、NE的含量。结果:与正常组比较,模型各亚组5-HT含量均明显升高,模型1、4、7次亚组NE含量均明显升高,模型1次亚组DA含量明显升高,模型10次亚组却明显降低。与相应模型亚组比较,艾灸4次亚组NE含量明显升高,艾灸7、10次亚组5-HT含量显著降低,DA、NE含量明显升高。结论:艾灸神阙穴可以通过调节反复力竭运动大鼠海马区神经递质,促进神经细胞的功能恢复,改善机体疲劳状态,为今后临床应用提供一定的实验基础。     

不同萃取方法对番茄籽油理化性质与脂肪酸含量的影响

分别采用超临界ＣＯ２萃取技术和传统溶剂法从番茄籽中萃取脂肪油，经过甲酯化处理后，用气相色谱法分析出亚油酸等５种脂肪酸占总脂肪酸的９８．５％．同时对油的其它理化指标进行了测定。对测定结果进行了比较分析，说明超临界ＣＯ２萃取所得番茄籽油品质更好。并对番茄籽油的营养学机理作了讨论。     

碘酸钾极谱体系测定西红柿中的微量硒

目的试验选择了碘酸钾极谱体系测定食品中微量硒的方法。方法采用微分电位溶出仪(MP-1型),进行了包括实验温度、反应时间以及试剂用量等选择试验。结果用于番茄样品微量硒的测定,所得线性回归方程为Y=12810X+8.7024,R2=0.9996。硒的最小检出量为0.08μg.L-1,相对标准偏差为1.3%,加标回收率为93%～99%。结论该方法测定食品中微量硒结果满意;该法具有灵敏度高,准确度好,仪器简单,操作简便等特点。