蜂蜜与蜂王浆敷料结合封闭式负压引流治疗压疮的效果观察及护理

目的观察蜂蜜与蜂王浆敷料结合封闭式负压引流治疗坏死溃疡期压疮的效果,并总结护理要点。方法选择2005年8月-2010年7月本院收治的60例坏死溃疡期压疮患者,按照入院的先后顺序分为观察组34例(36处压疮)和对照组26例(27处压疮)。对照组采用传统方法清创、换药和局部引流治疗,观察组在清创后应用蜂蜜与蜂王浆混合自制敷料结合封闭式负压引流治疗压疮,比较两组患者的疗效、肉芽组织生长时间、清创换药次数及治愈时间。结果观察组患者治疗总有效率高于对照组,清创换药次数少于对照组,肉芽组织生长时间及治愈时间短于对照组(均P<0.05)。结论蜂蜜与蜂王浆混合自制敷料结合负压封闭引流治疗坏死溃疡期压疮,具有疗效好、愈合时间短的优点,且价格便宜,值得临床推广应用。     

2009-2011年邹城市城区凉皮、凉粉、面筋中亚硝酸盐含量调查

[目的]了解2009～2011年邹城市城区凉皮、凉粉、面筋中亚硝酸盐含量。[方法]采用自制发明定性试纸条的制备方法,现场定性后实验室按GB／T5009．33—2008食品中亚硝酸盐与硝酸盐的测定定量。[结果]2009～2011年共检测了77份凉皮、53份面筋、20份凉粉中的亚硝酸盐含量,总的合格率为31．33％,其中2009年的合格率为24．53％;2010年的合格率为30．61％;2011年的合格率为39：58％。3年测定凉皮的亚硝酸盐含量平均值分别为36．10、30．80、19．70mg／kg,合格率分别为14．81％、19．23％、33．33％;面筋的平均值分别为25．30、22．60、9．20mg／kg,合格率分别为33．33％、41．18％、44．44％;凉粉的平均值分别为7．50、7．80、6．50mg／kg,合格率分别为3／8、3／6、3／6。凉皮平均值最高,合格率最低,面筋次之,但都逐年下降,合格率逐年升高。2009和2010年凉皮、面筋、凉粉进行比较,总的平均值差异无统计学意义（P〉0．05）,面筋、凉粉的合格率差异有统计学意义（P〈0．05）。2009和2010年与2011年凉皮、面筋、凉粉进行比较,凉皮、面筋平均值、合格率进行比较均差异有统计学意义（P〈0．01）;凉粉的平均值、合格率进行比较差异无统计学意义（P〉0．05）。[结论]邹城市城区凉皮、凉粉中应用亚硝酸盐很普遍,凉皮、凉粉合格率低,虽经宣传后合格率有所上升,但还是杜绝不了凉皮、凉粉中加含亚硝酸盐的明胶。     

不同消化方式对脐带华通胶间充质干细胞提取的影响

目的探讨使用不同酶浓度、消化不同时间及不同浓度胎牛血清的培养基稀释对消化脐带华通胶后提取培养间充质干细胞的影响。方法无菌条件下取出正常剖腹产胎儿脐带,剔除动脉、静脉及外膜,取其之间的胶状物,剪成1 mm3及更小的块状,分别加入0.1%和0.2%的Ⅱ型胶原酶,分为A、B两组,37℃水浴消化。每一组消化时间分别为4、81、2、16、202、4、28、323、6、40 h共10个消化时间点,每一个消化时间点又分为两组,分别为A1、A2组和B1、B2组。消化后的黏稠细胞悬液用完全DMEM稀释,并加入特级胎牛血清至浓度为10%(A1、B1)和20%(A2、B2),充分混匀。悬液移至75 cm2培养瓶,15 ml/瓶,37℃、5%CO2、饱和湿度培养,3 d换液。通过细胞计数观察原代细胞贴壁生长的最早时间和80%细胞铺满瓶底的时间和生长活力。结果 0.2%的Ⅱ型胶原酶消化脐带华通胶在消化时间为20h,且稀释血清浓度为20%时,获得的细胞悬液在培养36 h即有细胞贴壁,培养6 d细胞贴壁达到80%,其余各组细胞生长情况各不相等,均弱于此组。其中消化时间在8 h以内和32 h以上时不论使用多少的酶浓度和稀释时使用多少的血清浓度,细胞均不生长。结论在使用胶原酶消化脐带华通胶提取间充质干细胞的过程中,使用0.2%Ⅱ型胶原酶、消化16～24 h、稀释培养时加入至20%的血清对干细胞的提取效果最佳。     

盐酸利多卡因凝胶在老年患者置胃管中的应用研究

目的探讨盐酸利多卡因凝胶在老年患者置胃管中应用的效果.方法按随机数字表法将2008年10月至2010年9月老年消化内科85例留置胃管患者分为试验组(使用盐酸利多卡因凝胶)45例和对照组(使用液体石蜡油)40例,比较两组患者在胃管留置过程中的自觉疼痛程度、恶心及呕吐的情况.结果两组患者在胃管留置过程中的自觉疼痛程度、恶心...     

Different Etio-Pathogenesis in Intrauterine Growth Retardation in Developing Country: Histomorphometric Evidence

The present study was conducted to compare histomorphometric parameters of umbilical cord and its vessels in intrauterine growth retarded fetuses and normally grown fetuses. The study was conducted on umbilical cords taken from thirty intrauterine growth retarded (IUGR) fetuses and thirty normally grown fetuses (NGA). Histomorphometric parameters were measured with the help of planimeter. In the present study it was found that in cross section the total umbilical cord area in IUGR group is 59.00+3.67 mm² and in the control group is 75.91+4.77mm² (p<0.05). Jelly area in IUGR group is 47.90+3.11 mm² and in control group is 60.26+4.55 mm2 (p<0.05). Total vessel area in control group is 15.45+1.73 mm² and in IUGR group is 10.95±1.20mm² (p <0.05). Total umbilical cord area, umbilical cord jelly area and total vessel area are significantly lower in the IUGR group but no significant difference was observed in total luminal area, total area of blood vessels and wall thickness of umbilical blood vessels. So in the study it was found that histomorphometric parameters of umbilical cord of IUGR fetuses are significantly lower than that of normally grown fetuses of similar gestational age.     

Greek-origin royal jelly improves the lipid profile of postmenopausal women

Aim: Menopause transition is associated with chronic conditions such as osteoporosis and cardiovascular disease. Concerns about the long-term safety of menopausal hormone therapy make alternative natural methods an appealing approach to management. The aim of this study was to examine the effect of royal jelly (RJ) on cardiovascular and bone turnover markers in clinically healthy postmenopausal women.

Methods: A total of 36 postmenopausal healthy women were studied in a prospective follow-up study. Participants received 150?mg of RJ daily for three months. Circulating cardiovascular risk markers [lipid profile, antithrombin-III (ATIII), Protein C, Protein S, Plasminogen Activator Inhibitor-1 (PAI-1)] and bone turnover parameters [Total calcium, phosphate (P), parathormone (PTH), total type-1 Procollagen N-terminal (P1NP), Osteocalcin and serum collagen type 1 cross-linked C-telopeptide (CTX)] were compared between the baseline and the three-month visit.

Results: The RJ used in this study was particularly rich in medium chain fatty acids, compounds with hypolipidemic properties, which comprised 63% of the dry weight fatty content. RJ treatment resulted in a significant increase in high density lipoprotein – cholesterol (HDL-C 60.2?mg/dL?±?12.3 versus 64.7?mg/dL?±?13.9, 7.7% increase, p?=?0.0003), as well as in a significant decrease in low density lipoprotein – cholesterol (LDL-C, 143.9?±?37.5 versus 136.2?±?32, 4.1% decrease, p?=?0.011) and in total cholesterol (224.4?±?38.6 to 216.1?±?36.5, 3.09% decrease, p?=?0.018). No statistical significant changes were found in the remaining cardiovascular or the bone turnover parameters.

Conclusions: The intake of RJ 150?mg for three months is associated with significant improvements of the lipid profile of postmenopausal women. RJ supplementation may offer an alternative method of controlling the menopause – associated dyslipidemia.