梯度洗脱HPLC测定辛伐他汀片的含量和有关物质

目的建立辛伐他汀片含量测定及有关物质的检查方法。方法采用反相高效液相色谱法,色谱柱:SUPELCO C18柱(4.6mm×33mm,3μm),以乙腈-0.1%磷酸(50:50)为流动相A,0.1%磷酸乙腈溶液为流动相B,线性梯度洗脱,流速:3.0mL.min-1,检测波长238nm。结果辛伐他汀与洛伐他汀及强制破坏产生的降解产物均分离良好,辛伐他汀浓度在21.88～196.9μg.mL-1内,与峰面积呈良好的线性关系,回归方程A=65929ρ+4676,r=0.9996(n=7);日内精密度为0.54%(n=5);日间精密度为0.85%(n=5);平均回收率为99.55%(RSD=0.67%,n=9);样品溶液至少在3h内稳定;最低检测限为25.14ng.mL-1。结论该法专属性强,准确、灵敏,可用于辛伐他汀片的含量测定和有关物质检查。     

HPLC测定硫唑嘌呤片有关物质

目的采用高效液相色谱法测定硫唑嘌呤片有关物质。方法采用十八烷基硅烷键合硅胶色谱柱(200 mm×4.6 mm,5μm),以甲醇-0.05%醋酸钠溶液(18∶82)为流动相,流速为1.2 mL.min 1,检测波长为300 nm。结果硫唑嘌呤片中的2个典型杂质6-巯基嘌呤和5-氯-1-甲基-4-硝基咪唑的线性范围均为0.125~1.80μg.mL 1(r分别为0.999 7和0.999 9),检出限分别为0.787 ng和0.933 ng,平均回收率为100.3%(RSD=0.64%)和100.0%(RSD=0.14%)。结论高效液相色谱法测定硫唑嘌呤片有关物质简便、快速,测定结果准确、可靠。     

DRGs在我国医疗质量管理中的应用现状

疾病诊断相关分组(DRGs)实质是病例组合的一种,是按照临床过程同质资源消耗相近的原则,将不同病例分门别类的一种组合方法,最初应用于医疗保险支付,经过不断发展,逐渐运用于医院之间评估、医院内部质量控制、成本管理等方面.DRGs作为风险调整工具,具有客观、科学、全面性,相比传统的绩效评价更科学更客观,随着DRGs时代...     

加校正因子的主成分自身对照法同时测定非那雄胺片中2个杂质

目的：建立加校正因子的主成分自身对照法测定非那雄胺片中有关物质的含量。方法：CAPCELL PAK C₁₈色谱柱(4.6′250 mm,5 mm),流动相乙腈-水(45∶55),流速1.0 mL.min_-1,检测波长210 nm,进样量20 mL,柱温30℃。测定非那雄胺与杂质Ⅰ、Ⅱ的标准曲线方程,以斜率计算杂质相对于非那雄胺的校正因子,用相对保留时间确定各杂质的位置。结果：非那雄胺杂质Ⅰ、Ⅱ的相对保留时间分别为0.85与1.30,相对校正因子分别为2.40与0.69。结论：本方法可用于非那雄胺片中有关物质的定性及定量分析,采用加校正因子的主成分自身对照法可以更准确的反映其有关物质的含量。     

The prevalence and related factors for low health literacy in Xingtai: A cross-sectional survey in prefecture-level city

This study aimed to evaluate the low health literacy prevalence and its socio-demographic related factors in Xingtai.This study was a community-based, cross-sectional survey performed in Xingtai, with a sample size of 960. Participants’ socio-demographic characteristics were collected, and their health literacy status was evaluated by the questionnaire designed by the 2012 Chinese Resident Health Literacy Survey.There were 904 (94.2%) participants who provided valid questionnaires and they were included in the analyses. The mean health literacy score was 63.0 ± 16.6; for its subscales, the mean scores of health literacy of basic knowledge and concepts, lifestyle, and health-related skills were 31.6 ± 8.7, 17.1 ± 4.7, and 14.3 ± 3.9, respectively. Low total health literacy prevalence was 83.1%; as for its subscales, the prevalence of low health literacy of basic knowledge and concepts, lifestyle, and health-related skills was 72.5%, 87.8%, and 87.4%, respectively. Meanwhile, age, male and rural location were positively correlated, but education level and annual household income were negatively correlated with low health literacy risk. Further multivariate analysis revealed that lower education level was the only independent related factor for low total health literacy, and the most important independent related factor for low total health literacy of basic knowledge and concepts, lifestyle and health-related skills.Low health literacy prevalence is 83.1%, and lower education level is the most critical related factor for low health literacy in Xingtai.     

Evaluation of the Chinese Adult Strabismus-20 （CAS-20） questionnaire using Rasch analysis

ObjectiveTo further refine the Chinese Adult Strabismus-20 （CAS-20） health-related quality of life（HRQOL） questionnaire using Rasch analysis. MethodsIn this cross-sectional study, the CAS-20 questionnaire was independently completed by 246 adult strabismus patients. Based on the score for each item, Rasch analysis was performed on the original CAS-20 using the following aspects： dimensionality, response order, local dependence, infit and outfit analysis, differential item function, subject targeting and confirmatory dimensionality. ResultsThe original CAS-20 was divided into 4 subscales, which were labeled ″self-perception″, ″interaction″, ″reading function″, and ″general function″. Response ordering was appropriate for all of the 4 subscales. As a result of infit and outfit analysis, 3 items were removed, one each from ″self-perception″, ″reading function″, and ″general function″. No significant differential item function was found for sex or age. Compared with the ability of the patients and item difficulty, the 3 subscales matched well except for ″reading function″. ConclusionThe original CAS-20 was reduced to 17 items, and divided into 4 subscales, which were ″self-perception″, ″interaction″, ″reading function″, and ″general function″. Subject response choices saved 5 test options. The refined CAS-20 may prove to be more responsive to HRQOL changes in adult strabismus patients.